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Abstrak :
Indonesia memiliki potensi yang besar dalam memproduksi enzim untuk industri bioteknologi. Sayangnya, dengan sumber daya alam yang melimpah, enzim untuk industri bioteknologi masih diimpor. Perkembangan produksi enzim di Indonesia harus didukung untuk menurunkan tingkat impor enzim. Industri biodiesel merupakan salah satu industri bioteknologi yang memanfaatkan enzim untuk produknya. Lipase adalah enzim utama dan berperan sebagai biokatalis untuk produksi biodiesel. Produksi enzim lipase dari Rhizopus oryzae dikembangkan dengan menggunakan metode fermentasi solid state dan submerged untuk menghasilkan enzim lipase dalam jumlah tinggi dengan limbah pertanian yang dimanfaatkan sebagai substrat untuk produksi lipase. Kondisi optimum untuk produksi lipase ditemukan dengan berbagai waktu, konsentrasi substrat dan konsentrasi induser dari fermentasi. Waktu fermentasi divariasikan menjadi 1, 3, 5, dan 7 hari dengan variasi konsentrasi substrat 0.5, 1, 1.5, 2, and 2.5, substrat yang digunakan adalah dedak gandum. Dan variasi induser adalah 2, 4, 6, dan 8. Ekstraksi akan dilakukan melalui kain muslin, sentrifugasi dan disaring dengan kertas saring. Lipase yang dihasilkan adalah enzim lipase basah dan kering. Titrasi digunakan sebagai uji enzimatik untuk aktivitas lipase. Dengan kondisi optimum dari konsentrasi inducer 6,9, konsentrasi substrat 1,9 dan 3,5 hari periode fermentasi. Aktivitas unit yang dihasilkan dari lipase 62,67 U/ml dan 50 U/ml untuk Submerged Fermentasi dan Solid-State Fermentation masing-masing. Dengan hasil sintesis biodiesel sebesar 38,11 melalui rute non-alkohol. ......Indonesia has huge potentials on producing enzymes for biotechnology industries. Unfortunately, with abundant natural resources, the enzymes for biotechnology industries were still imported. The development of enzyme production in Indonesia should be supported in order to reduce the import level of enzymes. Biodiesel industry is one of the biotechnology industries that utilizes enzyme for their product. Lipase is the main enzyme and act as the biocatalyst for the production of biodiesel. The production of lipase enzyme from Rhizopus oryzae are developed using the Solid State fermentation and Submerged fermentation method in order to yield high amount of lipase enzyme with the agricultural waste is utilize as the substrate for the lipase production. The optimum condition for the production of lipase is discovered by varying time, substrate concentration and inducer concentration of the fermentation. The time of fermentation is 1, 3, 5, and 7 days with substrate concentration variation of 0.5, 1, 1.5, 2, and 2.5, the substrate used is Wheat Bran. And the variation of the inducer is 2, 4, 6, and 8. The extraction will be done by squeezing the suspension through muslin cloth, centrifugation and filtered by filter paper. Titration is used as the enzymatic assay for the lipase activity. Under optimum condition of 6.9 inducer concentration, 1.9 substrate concentration and 3.5 day of fermentation period. Resulting unit activity of lipase of 62.67 U ml and 50 U ml for Submerged Fermentation and Solid State Fermentation respectively. With biodiesel synthesis yield of 38.11 through non alcohol route.

Abstrak :
Agricultural waste has increased colossally with development in agricultural production causing environmental nuisance and degradation. Utilization of coconut husks, one of such type of waste, as a biosorbent for polluted surface water treatment, was considered in this study. Polluted surface water was gently passed through two similar columns loaded with 100 and 200 g of coconut husk char respectively. The treated water samples collected after 30, 60, 90, 120 and 150 mins were examined for SO42-, NO3- and PO43- ions concentration. Removal efficiency for SO42-, NO3- and PO43- ions on 100 g coconut husk char after 150 mins detention time were 70, 78 and 91% respectively. Freundlich isotherm model gave a better description of the data (R2 > 0.96). Sorption data was well described by second order pseudo kinetics (R2 > 0.85). An amount of 100 g of the biosorbent has a strong affinity for these types of ion removal in contaminated water. Coconut husk char as a biosorbent is a panacea to significant concentrations of polyatomic ions in polluted surface water.

Abstrak :
Agricultural waste has increased colossally with development in agricultural production causing environmental nuisance and degradation. Utilization of coconut husks, one of such type of waste, as a biosorbent for polluted surface water treatment, was considered in this study. Polluted surface water was gently passed through two similar columns loaded with 100 and 200 g of coconut husk char respectively. The treated water samples collected after 30, 60, 90, 120 and 150 mins were examined for SO42-, NO3- and PO43- ions concentration. Removal efficiency for SO42-, NO3- and PO43- ions on 100 g coconut husk char after 150 mins detention time were 70, 78 and 91% respectively. Freundlich isotherm model gave a better description of the data (R2 > 0.96). Sorption data was well described by second order pseudo kinetics (R2 > 0.85). An amount of 100 g of the biosorbent has a strong affinity for these types of ion removal in contaminated water. Coconut husk char as a biosorbent is a panacea to significant concentrations of polyatomic ions in polluted surface water.