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"Information on salivary characteristics of young subjects with different body composition is scarce. Thus, the aim of this pilot study was to assess salivary characteristics of normal-weight, overweight and obese children. This is a basic research design in which 68 children (5-12 years) were recruited and anthropometric measurements consisted of body mass index (BMI = Kg/m2), body perimeters (waist/arm circumferences) and subcutaneous fat tissue (triceps/subscapular thicknesses). Stimulated (SS) and unstimulated morning saliva (US) were collected to determine flow rate, pH and triglycerides, urea, alpha-amylase, total protein, phosphate and calcium concentrations. Data were analyzed using normality tests, t test/Wilcoxon, one-way ANOVA/Kruskal-Wallis and Pearson’s/Spearman’s correlation tests, where appropriate. Results: Age, household income, parents’ education, saliva flow and pH did not differ among groups. Waist circumference and subscapular skinfold differed significantly between normal-weight and obese groups; only waist circumference showed significant correlation with BMI in all groups. pH increased significantly from US to SS in all groups; but flow rate increased from US to SS only in normal-weight and overweight groups. Total protein, amylase, urea, phosphate, triglyceride and calcium concentrations did not differ among groups. However, urea, phosphate and calcium concentrations differed significantly between US and SS in the normal-weight and overweight groups, with the lowest values for SS. In the overweight group, total protein also differed between saliva samples and obese group showed no difference in biochemical parameters between US and SS. Finally, some salivary characteristics may vary among normal-weight, overweight and obese children; thus, future studies in a larger sample are needed to fully understand salivary secretion and composition of these subjects."

"ABSTRAK Menemukan suatu metode pengukuran objektif mengenai rasa sakit pada anak merupakan tantangan bagi dokter gigi anak selama melakukan perawatan. Wong Baker Faces Pain Scale adalah instrumen pengukur rasa sakit metode self report yang telah teruji validitas dan reliabilitasnya. Alfa amilase saliva adalah biomarker dalam saliva yang dipengaruhi oleh sistem saraf simpatis dalam eksresi nya. Prosedur anestesi lokal injeksi dapat menimbulkan rasa sakit pada anak. Rasa sakit menstimulasi sistem saraf simpatis akibat adanya stimulus noksius pada reseptor. Tujuan penelitian ini adalah untuk menganalisa hubungan kadar alfa amilase saliva dengan skor Wong Baker Faces Pain Scale pada anak saat mendapatkan anestesi lokal injeksi selama prosedur ekstraksi gigi sulung. Kadar alfa amilase saliva diukur dengan menggunakan portable device Nipro Cocoro meter, yang teruji memiliki nilai pengukuran mendekati analisis laboratoris. Kadar Alfa amilase saliva pertama diukur sesaat setelah injeksi anestesi lokal, dilanjutkan dengan pengukuran kedua pada waktu 10 menit setelah injeksi. Anak diminta menunjukkan rasa sakit yang dirasakan pada saat injeksi anestesi lokal dengan menggunakan instrumen Wong Baker Faces Pain Scale. Analisis data menggunakan uji Spearman. Nilai alfa amilase saliva ditemukan berkorelasi positif dengan skor Wong Baker Faces Pain Scale p le;0,05 dengan koefisien korelasi r=0.445 . Penelitian ini menunjukkan bahwa alfa amilase saliva berkorelasi dengan rasa sakit pada saat pemberian anestesi lokal injeksi sehingga diharapkan alfa amilase saliva dapat digunakan sebagai biomarker akan rasa sakit.ABSTRACT Finding an objective measurement of pain is a challenge for pediatric dentist. Wong Baker Faces Pain Scale is commonly used instrument to assess pain intensity in children. Salivary alpha amylase is biomarker in saliva which secreted by stimulation of sympathetic nervous system. Local anesthesia injection procedure stimulate pain in children. The aim of this study was to analyze the correlation between Wong Baker Faces Pain Scale and Salivary Alpha Amylase level during primary tooth extraction procedure with local anesthetic injection in children aged 6 12 years. From all children, saliva was collected with a disposable saliva strip, shortly after local anesthetic injection and at 10 minutes after injection. Level of salivary alpha amylase then determined using portable Nipro Cocoro Meter device. The Wong Baker Faces Pain Scale was measured at the same time. The correlation between Wong Baker Faces Pain Scale and salivary alpha amylase level was analyzed with Spearman Correlation test. There was a significant correlation between Wong Baker Faces Pain Scale and Salivary Alpha Amylase level p le 0,05 with correlation coefficient r 0.445 . This study showed that salivary alpha amylase was correlated with pain during procedure of anesthesia local injection. Our data suggest that salivary alpha amylase level might be a good index for objective pain intensity assessment."

"Fisiologi saliva: sebuah ringkasan. Kondisi fisiologi saliva mempunyai peran penting dalam kehidupan manusia. Perubahan yang terjadi pada usia, kondisi lingkungan, kehidupan dan pola makan mempengaruhi ada atau tidaknya gangguan fungsi saliva. Saliva berperan dalam menentukan pola pertumbuhan dan kesehatan gigi di dalam rongga mulut. Saliva merupakan cairan unik yang berfungsi dalam mekanisme pertahanan utama mikroorganisme yang ada di dalam rongga mulut. Penelitian tentang fisiologi saliva, selama ini sepertinya dipisahkan pendidikan profesi kedokteran dan kedokteran gigi. Oleh karena itu, diperlukan pengertian yang lebih baik dari tentang saliva secaramenyeluruh dalam kondisi sehat dan sakit. Saat ini telah banyak penelitian yang dilakukan untuk menggunakan saliva untuk mencari penanda diagnosis suatu penyakit. Analisis saliva juga diharapkan dapat memberikan gambaran tentang status kepenyakitan dan membantu untuk pencegahan, monitoring dan perawatan. Telaah singkat ini dibuat untuk memberikan pengertian tentang fisiologi saliva untuk menambah wawasan baru tentang fisiologi saliva dan aplikasinya dalam bidang kedokteran dan kedokteran gigi.

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Physiology of saliva evolves in supporting many important activities in human life. Changes in age, environmental and living condition as well as eating habit would influence salivary function. Saliva palys a role in determining the development pattern and oral health. Saliva has a unique function in the defense mechanism to microorganism in the oral environment. Research focuses on the salivary physiology is limited and seems to be separated from medical and dental professional education. Better and deeper comprehensive understanding of saliva in healthand disease is needed. Nowadays, many studies have used saliva to find diagnosis markers for specific diseases. Salivary analysis is intended to give descriptive information on disease status for prevention, monitoring and treatment purposes. This brief review aimed to give understanding on salivary physiology in order to add new views of its application in the field of medicine and dentistry."

"Background: Recently, honey has been widely used as a sweetener. Along with the people's awareness to control the intake of calorie, the consumption of lowcalorie sweetener is increasing as well. How much these sweeteners contribute to caries process, however, are still unknown. Objective: To compare the changes of viscosity, pH, and buffering capacity of saliva after consuming water containing honey and low calorie sweetener. Method: Each research subject aged 20-22 years old was asked to consume 150 ml water that contained 17 grams of honey or 2,5 grams of low-calorie sweetener in different day, and waited for 10 minutes before conducting the viscosity, pH, and buffering capacity of saliva?s test. Results: The data was analyzed by Wilcoxon test with 0,05 level of significance. The results obtained were the significant decreases in values of viscosity and buffering capacity of saliva before and after consuming water containing honey and water containing low-calorie sweetener, significant decrease in pH value before and after consuming water containing honey, no significant decrease in pH value before and after consuming water containing low-calorie sweetener. In addition, there were no significant differences in values of viscosity, pH, and buffering capacity between the groups consuming water containing honey and low-calorie sweetener. Conclusion: There were significant differences in viscosity and buffering capacity of saliva before and after onsuming water containing honey and water containing low-calorie sweetener. Meanwhile, there was significant difference for the pH value before and after consuming water containing honey, while there was no significant difference for the pH value before and after consuming water containing low-calorie sweetener. However, there were no significant differences in viscosity, pH, and buffering capacity of saliva after consuming water containing honey and water containing low-calorie sweetener."

"Tujuan: Penelitian ini bertujuan untuk mengetahui perbandingan pengaruh saliva buatan dengan pH 4,5 terhadap kekerasan dari material restoratif bioaktif. Metode penelitian: Dalam penelitian ini dilakukan penelitian menggunakan material restorative bioaktif, Activa Bioactive (RMGI), Cention-N (RK Alkasit), Fuji II LC (RMGIC), Zirconomer (Zirconia reinforced Glass Ionomer) , dan Beautifill II LS (Giomer). Masing-masing specimen material tersebut dengan ukuran diameter 15 mm dengan tinggi 1 mm direndam dalam saliva buatan pH 4,5 selama 7 hari. Setelah 7 hari, specimen dilakukan uji kekerasan menggunakan Knoop Hardness test dengan 5 jejas per spesimen. Kemudian hasil pengujian dilakukan uji normalitas Shapiro-Wilk, dilanjutkan dengan One-Way Anova. Lalu dilakukan uji homogenitas Levene dan dilanjutkan uji Post-hoc Tamhane. Hasil: Terdapat perbedaan bermakna pada nilai kekerasan di antara material restoratif bioaktif yang diuji (One way anova, p<0,05). Dengan nilai tertinggi pada material Cention-N dan terendah material Activa Bioactive. Pada uji Post-hoc Tamhane didapati perbedaan bermakna, kecuali antara Beautifill II LS dengan Zirconomer. Kesimpulan: Setelah dilakukan perendaman pada saliva dengan pH 4,5, material Cention N memiliki nilai kekerasan tertinggi dan Material Activa yang terendah.

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Objective: This study aims to compare the effect of artificial saliva with pH 4.5 on the microhardness of bioactive restorative materials. Method: In this study, research was carried out using restorative bioactive materials, Activa Bioactive (RMGI), Cention N (RK Alkasit), Fuji II LC (RMGIC), Zirconomer (Zirconia reinforced Glass Ionomer), and Beautifill II LS (Giomer). Each specimen of the materials are made with a diameter of 15 mm and a height of 1 mm, and were immersed in artificial saliva pH 4.5 for 7 days. After 7 days the material was subjected to a microhardness tester using the Knoop Hardness test with 5 per specimen of materials. Statistic analysis were performed using Shapiro-Wilk normality test, followed by One-Way Anova. Then the Levene homogeneity test was carried out and continued with the Post-hoc Tamhane test. Result: There was a significant difference in the hardness value between bioactive restorative materials (One way ANOVA, p <0.05). With the highest value for the Cention-N material and the lowest for Activa Bioactive material. In the Post-hoc Tamhane test, there was a significant difference, except between Beautifill II LS and Zirconomer. Conclusion: After soaking in saliva with a pH of 4.5, the Cention N material had the highest hardness value and the lowest Activa Material."

"Penelitian ini bertujuan untuk mengetahui pengaruh lama perendaman dalam saliva buatan terhadap perubahan pH-nya dan kekasaran permukaan resin komposit Giomer Bulk-Fill. Jumlah spesimen 63 buah dibagi dalam sembilan kelompok perlakuan yaitu perendaman dalam saliva buatan pH 7; 5,5; dan 4,5 selama masing-masing 1 jam, 24 jam, dan 72 jam pada suhu 370C. Perubahan pH saliva buatan diukur menggunakan pH meter dan pengujian kekasaran permukaan spesimen menggunakan surface roughness tester. Hasil menunjukkan bahwa terdapat perbedaan yang bermakna pada setiap kelompok perlakuan.

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This study aimed to determine the effect of immersion time on artificial saliva pH changes and surface roughness of Giomer Bulk Fill composite resin. Sixty three specimens were divided into nine groups with immersion in artificial saliva pH 7, 5.5, and 4.5 for 1 hour, 24 hours, and 72 hours at 370C respectively. The changes in artificial saliva pH were measured using a pH meter and the surface roughness measured using surface roughness tester. The results showed that there were significant differences between each groups."

"ABSTRAKSilver Diamine Fluoride memiliki kandungan ion fluor yang dapat menaikkan konsenstrasi ion fluor, meningkatkan potensi remineralisasi lesi enamel dan menghasilkan kenaikan bioavailibitas konsentrasi ion fluor pada saliva. Tujuan dari penelitian ini adalah menganalisis kandungan ion fluor dalam saliva sebelum dan setelah aplikasi Silver Diamine Flouride. Metode yang dilakukan adalah stimulated saliva dikumpulkan untuk kemudian diukur kandungan ion fluor nya dengan menggunakan alat ion selective electrode. Hasil penelitian menunjukkan bahwa terdapat perbedaan kandungan ion fluor pada saliva yang bermakna p < 0,05 antara kelompok waktu sebelum, sesaat setelah, dan 1 jam setelah aplikasi SDF. Terdapat hubungan antara konsentrasi kelompok waktu sebelum, sesaat setelah, dan 1 jam setelah aplikasi SDF dengan berat badan subjek. Dapat disimpulkan bahwa konsentrasi ion fluor saliva mengalami peningkatan sesaat setelah aplikasi SDF, dan kemudian akan turun kembali ke konsentrasi awal 1 jam setelah aplikasi.

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ABSTRAKSilver Diamine Fluoride is consist fluoride that can increase fluoride concetration in saliva, remineralization process, and bioavailability of fluoride in saliva. The purpose of this study was t o analyze the difference of fluoride concentration in saliva between before and after Silver Diamine Fluoride application on enamel. The methods is to collect Stimulated saliva of 4 subjects and then measure the concentration of fluoride using ion selective electrode. The results showed that there were significant differences in the phosphate concentration."

"Latar Belakang: Karies yang menyerang anak-anak dibawah 71 bulan dikenal dengan Early childhood caries ECC . Salah satu bakteri yang mendominasi penyebab ECC adalah Streptococcus mutans dan sistem imun yang berperan dalam pencegahan karies adalah IgA. Tujuan: Menganalisis hubungan level IgA anti Streptococcus mutans serotype f dengan viskositas dan dmft pada stimulated saliva dan unstimulated saliva pasien ECC. Metode: Level IgA anti-S. mutans serotype f di ukur menggunakan metode ELISA. Hasil: Analisis stastistik dengan uji Spearman didapatkan korelasi negatif antara level IgA anti-S. mutans serotype f dan indeks dmft, pada stimulated saliva r= -0.471; p=0.286 dan pada unstimulated saliva r= -0.529; p=0.408 , hasil korelasi antara level IgA anti-S. mutans serotype f dan viskositas stimulated saliva adalah korelasi positif r=0,417; p=0.352 . Level IgA anti-S. mutans stimulated saliva lebih rendah daripada unstimulated saliva P=0.127. Kesimpulan: Terdapat hubungan negatif antara level IgA anti-S.mutans serotype f dengan indeks dmft pada stimulated saliva dan unstimulated saliva pasien ECC, serta terdapat korelasi positif antara level IgA anti-S.mutans serotype f dengan viskositas saliva pada stimulated saliva, tetap secara statisik tidak bermakna. Level IgA anti-S. mutans stimulated saliva lebih rendah daripada unstimulated saliva tetapi tidak terdapat perbedaan bermakna antara level IgA anti-S. mutans serotype f stimulated saliva dan unstimulated saliva.

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Background: Early childhood caries ECC is caries which affects in children aged 71 months or younger. One of the bacteria that dominates the formation of ECC is Streptococcus mutans. The immune system that plays a role in the formation of caries is IgA.

Objective: To analyze the correlation between level of IgA anti Streptococcus mutans serotype f with viscosity and dmft in stimulated saliva and unstimulated saliva of ECC patients.

Methods: Level of IgA anti S. mutans serotype f was measured using ELISA method. Results based on Spearman test, there was a negative correlation between level of IgA anti S. mutans serotype f and dmft index in stimulated saliva r 0.471 p 0.286 and unstimulated saliva r 0.529 p 0.408.

Results: The result correlation levels of IgA anti S. mutans serotype f and viscosity of saliva was positive r 0.417 p 0.352 . Level of IgA anti S. mutans serotype f in stimulated saliva was lower than unstimulated saliva p 0.127.

Conclusion: There was a negative correlation between the levels of IgA anti S. mutans serotype f and dmft index in saliva stimulated and unstimulated saliva of ECC patients and positive correlation between the levels of IgA anti S. mutans serotype f and viscosity of stimulated saliva. However, there were no significantly difference. The levels of IgA anti S. mutans serotype f stimulated was lower than unstimulated saliva, but not significantly difference."

"ABSTRACTLatar Belakang : Nitric oxide (NO) merupakan sistem pertahanan non spesifik yang berperan sebagai antibakterial di dalam rongga mulut yang dapat ditemukan di dalam saliva. Tujuan : Mengetahui hubungan konsentrasi NO sebagai sistem pertahanan non spesifik dengan derajat kebersihan gigi dan mulut yang dinilai melalui skor OHI-S. Metode : Sampel yang diteliti adalah saliva unstimulated dan diukur dengan metode Griess Reagent pada 50 subjek dewasa muda yang terdiri dari dua kelompok, yaitu 25 subjek perokok dan 25 subjek non perokok. Indeks skor OHI-S dikategorikan menjadi baik, sedang, dan buruk. Metode analisis yang digunakan adalah uji statistik Independent T-test, dan korelasi Spearman. Hasil : Konsentrasi NO saliva pada dewasa muda perokok lebih tinggi dari non perokok dengan adanya perbedaan bermakna (p < 0,05) dan hubungan antara konsentrasi NO dengan skor indeks OHI-S adalah positif sedang dengan tidak ada hubungan yang signifikan (r = 0,305, p > 0,05). Kesimpulan : Konsentrasi nitric oxide saliva pada perokok meningkat diakibatkan oleh kondisi kebersihan rongga mulut subjek yang buruk.

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ABSTRACT Background: Nitric oxide (NO) is a non-specific defense system that acts as an antibacterial in the oral cavity which can be found in saliva. Objective: To determine the relationship between NO concentration as a non-specific defense system and the degree of oral hygiene as assessed by the OHI-S score. Methods: The sample studied was unstimulated saliva and measured by the Griess Reagent method on 50 young adult subjects consisting of two groups, namely 25 smoking subjects and 25 non-smoking subjects. The OHI-S score index was categorized into good, moderate, and bad. The analytical method used is the Independent T-test statistical test, and the Spearman correlation. Results: NO salivary concentration in young adult smokers was higher than non-smokers with a significant difference (p <0.05) and the relationship between NO concentration and OHI-S index score was moderate positive with no significant relationship (r = 0.305, p> 0.05). Conclusion: The concentration of salivary nitric oxide in smokers is increased due to poor oral hygiene conditions in the subject."