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Abstrak :
Suhu permukaan bumi umumnya berbeda sesuai lokasi geografis, salah satunya dipengaruhi oleh ketinggian wilayah. Wilayah Universitas Indonesia UI terletak pada ketinggian 50--140 m dpl dengan suhu rata-rata 28,6 C, sementara wilayah Kebun Raya Cibodas KRC terletak pada ketinggian 1.300--1.425 m dpl dengan suhu rata-rata 20,06oC. Perbedaan suhu diduga dapat memengaruhi respons tumbuhan seperti ekspresi gen heat shock protein Hsp 70. Penelitian bertujuan untuk mengetahui ekspresi gen Hsp70 pada Emilia sonchifolia dan Sphagneticola trilobata yang berasal dari UI dan KRC. Penelitian dilakukan dengan mengisolasi RNA dari daun muda Emilia sonchifolia dan Sphagneticola trilobata UI dan KRC, diubah menjadi complementary DNA cDNA dengan tektik reverse transcription, dan diamplifikasi dengan teknik polymerase chain reaction PCR menggunakan primer Hsp70 Arabidopsis thaliana. Hasil amplifikasi kemudian di-sequencing dan dianalisis dengan teknik in-silico. Hasil amplifikasi menunjukkan bahwa terdapat produk parsial gen Hsp70. Penyejajaran urutan basa nukleotida antara keempat sampel yang diteliti dengan gen Hsp70 dari spesies referensi Arabidopsis thaliana menunjukkan adanya kesamaan secara parsial, dan perbedaan satu basa nukleotida posisi ke-65 yang tidak berpengaruh pada perubahan asam amino. Hasil penelitian mengindikasikan bahwa gen Hsp70 terekspresi pada Emilia sonchifolia dan Sphagneticola trilobata yang tumbuh di UI dan KRC. ......The temperature of earth surface is generally different according to geographical location, one of which is influenced by the altitude. Universitas Indonesia UI is located at an altitude of 50 140 m amsl with an average temperature of 28.6 C, while Kebun Raya Cibodas KRC located at an altitude of 1.300 1.425 m amsl with an average temperature of 20,06 C. Temperature differences are thought to affect plant responses, such as the expression of heat shock protein Hsp 70 genes. The research aims to find out the expression of Hsp70 genes on Emilia sonchifolia and Sphagneticola trilobata derived from UI and KRC. The study was conducted by isolating RNA from young leaves of Emilia sonchifolia and Sphagneticola trilobata collected from UI and KRC, then converted into complementary DNA cDNA. The cDNA product was further amplified by polymerase chain reaction PCR using Hsp70 Arabidopsis thaliana primer. The amplification products then sequenced and analyzed by in silico techniques. The results of amplification show that there is partial product of the Hsp70 gene. The sequencing results show a nucleotide variation in the 65th base which has no effect on amino acid changes. The results indicate Hsp70 gene is expressed in Emilia sonchifolia and Sphagneticola trilobata grown in UI and KRC.

Abstrak :
Indonesian Journal of Dentistry 2006; Edisi Khusus KPPIKG XIV: 435-438 Heat Shock Protein (HSP) is a molecular chaperone that prevents stress induced aggregation of partially denatured proteins and promotes their return to native confimations when the condition is favorable. As molecular chaperones, the HSP protect protein structure and activity, thereby preventing disease, but they may contribute to cell malfunction if they are perturbed. In inracellular quantities and cellular localizalion of HSP was changed in response to anoxia/hypoxia, heat and oxidation, and in relation to pathological status. This review discuses the role of HSP in several human medical condition particularly in oral mucosa.

Abstrak :
Asteraceae merupakan salah satu famili tumbuhan yang bersifat invasif. Tumbuhan dengan sifat invasif dapat lebih mentoleransi suhu tinggi akibat adanya peran mekanisme Hsp70. Gen Hsp70 merupakan gen yang terkonservasi didalam sel sebagai bentuk adaptasi terhadap beberapa cekaman lingkungan, salah satunya adalah cekaman suhu. Perbedaan suhu rata-rata antara Universitas Indonesia-Depok yaitu 28,6oC dan Kebun Raya Cibodas dengan suhu rata-rata 20,06 oC menjadi dasar dilakukannya penelitian untuk melihat tingkat ekspresi gen Hsp70 pada Ageratum conyzoides dan Synedrella nodiflora. Penelitian diawali dengan isolasi RNA dari organ daun dengan metode CTAB yang telah dimodifikasi dan dilanjutkan dengan sintesis cDNA. Hasil dari cDNA diamplifikasi dengan PCR menggunakan primer Hsp70 yang digunakan oleh Sung 2001 pada Arabidopsis thaliana. Hasil penelitian menunjukkan adanya amplifikasi gen Hsp70 secara parsial, serta adanya variasi basa nukleotida pada sampel lokasi Universitas Indonesia dan Cibodas pada posisi basa ke 110 dan 108 yang mengakibatkan adanya perbedaan pembacaan asam amino. Namun perbedaan asam amino tersebut tidak membuat perubahan prediksi struktur protein pada sampel. ......Asteraceae is one of the invasive plant family. Invasive plants have more ability to tolerating high temperatures with the role of the Hsp70 mechanism. The Hsp70 gene is conserved in cells as a form of adaptation to some environmental stresses, one of which is temperature stress. The difference of average temperature between Universitas Indonesia Depok which is 28,6oC and Cibodas Botanical Garden with average temperature 20,06oC become the basis of this research to see the level of Hsp70 gene expression in Ageratum conyzoides and Synedrella nodiflora plants at two locations with different temperature. The research begins with RNA isolation from the leaf tissues with modified CTAB method followed by cDNA synthesis. The cDNA then amplified by PCR using a Hsp70 primer used by Sung 2001 in Arabidopsis thaliana. The results showed that there is a partial amplification of the Hsp70 gene, as well as the presence of nucleotide base variations in Universitas Indonesia Depok and Cibodas at base positions number 110 and 108 which resulted in differences in amino acid readings. However, these amino acid differences do not make a change in protein structure prediction of the samples.

Abstrak :
Cell in the distress situation, denaturation of proteins may occur, and may also respond by expressing stress proteins. However, such homeostatis effort does not always succeed and even may lead to disease, including cancer. In distress situation also ensue much protein misfolding. Objective: This research were to explain the role of heat shock protein 40 (Hsp40) and HSP20 in pathogenesis of occured oral squamous cell carcinoma (OSSC) patient which realized human papiloma virus (HPV) infection. Material and method: Tissue biopsy frozen section were taken from BOSC and OSCC patients was cut into three part. Parrafin blocks were made from cutting I, which was subsequently stains with HE to ascertain the type of neoplasm. Cutting II was subjected to DNA isolation. The DNA isolation results were subjected to PCR to amplify L1-HPV gene for fixed the HPV stressor. Protein isolation was treated from cutting III, followed with Blottdot test by using anibody monoclonal anti Hsp40 and Hsp 70 and continued with measurement using densitometer to find the concentration of Hsp40 and Hsp70. The collected data were analyzed with F Test (Manova) and discriminant analysis. Result: This experiment showed the differences in concentration of Hsp40 (p<=0,070) and Hsp70 (p<=0,006) between benign oral squamous cell (BOSC) and OSCC patients which realized HPV infection. Conclusion: This experiment proved that OSCC patients which realized HPV infection indicated an up regulated of Hsp70 concentration, so that there was occurs misfolding of the proteins cell. The misfolding was ensue obstacle of apoptosis and to raise cell poliferation which to storm carcinogenesis. An up regulated of Hsp40 was role as co-chaperone.

Abstrak :
Mortalin biology : life, stress and death” is a remarkable compilation of the research outcomes on the stress protein mortalin, a member of heat shock 70 family of proteins. The book is unique as it describes mortalin playing essential role in life, stress response and death either from cancer, when it becomes hyperactive or from neuro-degeneration, when it becomes hypoactive. The book provides up-to-date knowledge on mortalin with respect to its discovery, structure, evolutionary conservation, function and signal transduction in different organisms in a simple, but most comprehensive way, that besides offering an enjoyable and in-depth reading, prompts the reader to ask further questions to explore this protein with new ideas, approaches and experiments. Twenty-one chapters by the world leaders on the specific areas of mortalin research throw light on its multi-functionality, potentials for biotechnology, diagnostics and therapeutic values. Avenues of mortalin biology, yet unexplored, hold immense promises for future, and reading this volume provides an easy, enthusiastic and energetic head-on start.

Abstrak :
Latar belakang: Peningkatan suhu tubuh ekstrim menyebabkan denaturasi protein, terhentinya reaksi enzimatik, hilangnya aktivitas dan integritas membran, serta memicu terjadinya kerusakan sel. Peningkatan suhu tubuh juga mempengaruhi terjadinya efek inotropik dan kronotropik positif pada jantung. Diperkirakan bahwa pajanan panas dapat meningkatkan ekspresi protein Transient Receptor Potential Vanilloid 1 (TRPV1), Heat Shock Factor 1 (HSF1) dan Heat Shock Protein 70 (Hsp70) pada kardiomiosit berperan penting dalam proses termotoleran dan aklimatisasi terhadap panas serta berguna sebagai mekanisme adaptasi secara sistemik dan seluler. Tujuan dari penelitian adalah untuk menganalisis ekspresi TRPV1, HSF1, dan Hsp70 pada jantung sebagai respons protektif terhadap pajanan panas. Metode: Penelitian bersifat eksperimental in vivo menggunakan hewan coba tikus jenis Sprague Dawley (SD) berumur 12 minggu dengan berat badan 200-300 gram di laboratorium hewan Balitbangkes Kemenkes RI, pada Oktober-Desember 2014. Sebanyak 28 ekor tikus jantan dengan n=4 pada tiap kelompoknya, dibagi dalam kelompok Kontrol (K) dan kelompok Perlakuan (P). Kelompok perlakuan terdiri dari 6 subkelompok (kelompok hari ke-1,3,7,10,14 dan 21) mendapatkan pajanan panas di dalam hyperthermic chamber bersuhu (45oC ± 0.3oC) dan kelembaban relatif (70% ± 3%) selama 60 menit. Dilakukan pengukuran berat badan, suhu kulit, suhu rektal dan frekuensi denyut jantung. Perubahan morfologi kardiomiosit diamati menggunakan pewarnaan Hematoksilin-Eosin. Ekspresi TRPV1, HSF1 dan Hsp70 diperiksa menggunakan metode imunohistokimia dan ELISA. Hasil: Penelitian menunjukan pajanan panas 45oC; kelembaban relative 70% selama 60 menit menyebabkan penurunan berat badan sejak hari ke-1 hingga hari ke-21 perlakuan. Terjadi peningkatan suhu kulit, suhu rektal dan heart rate yang puncaknya terjadi pada hari ke-7, dan menurun mulai pada hari ke-10 sampai pada hari ke-21 meskipun intensitas pajanan panas tetap sama. Hal tersebut menandakan mekanisme aklimatisasi dan proses termotoleransi telah terjadi pada hari ke-7 perlakuan. Terjadi penambahan ukuran lebar kardiomiosit dan peningkatan berat pada jantung seiring lamanya pajanan panas, Hasil ini menunjukkan terjadinya hipertrofi jantung namun tidak disertai adanya fibrosis. Secara molekuler melalui pemeriksaan Imunohistokimia dan ELISA pada kardiomiosit menunjukkan ekspresi TRPV1, HSF1 dan Hsp70 yang bersifat sebagai protein protektif dan kardioprotektor cenderung mengalami peningkatan sejak hari ke-1 sampai pada hari ke-7 perlakuan dan cenderung menurun pada hari ke-10 sampai dengan hari ke-21. Perubahan kadar ekspresi TRPV1, HSF1 dan Hsp70 sejalan dengan perubahan yang terjadi pada suhu kulit, suhu rektal dan heart rate. Kesimpulan: Pajanan panas pada tubuh memberikan pengaruh pada jantung berupa terjadinya hipertrofi konsentris disertai adanya peningkatan ekspresi TRPV1, HSF1 dan Hsp70 yang berperan penting sebagai protein protektif dan kardioprotektor Background: Increased extreme body temperature causes protein denaturation, cessation of enzymatic reactions, loss of membrane activity and integrity, and triggers cellular damage. Increased body temperature also affects the occurrence of positive inotropic and chronotropic effects on the heart. It is postulated that increase in expression Transient Receptor Potential Vanilloid 1 (TRPV1), Heat Shock Factor 1 (HSF1), Heat Shock Protein 70 (Hsp70) in cardiomyocytes is activated by extreme temperatures and has an important role in thermotolerance and heat acclimatization processes -and as a mechanism of systemic and cellular adaptation. The aim of the study was to analyze the expression of TRPV1, HSF1, and Hsp70 on cardiac muscle as a protective response to heat exposure. Methods: This in vivo experimental research was conducted using Sprague-Dawley (SD) rats (age 12 weeks, 200-300 gram) in animal laboratory National Institute of Health Research and Development, Indonesian Ministry of Health, October-December 2014. A total of 28 male rats with n = 4 in each group, divided into Control group (K) and Treatment group (P). The treatment group consisted of 6 sub-groups (i.e. 1,3,7,10,14 and 21 days) received heat exposure in hyperthermic chamber at (45oC ± 0.3oC) and (70% ± 3%) with relative humidity of 60 minutes. Body weight, skin temperature, rectal temperature and heart rate were measured. Changes in cardiomyocyte morphology were observed using Hematoxylin-Eosin staining. Expressions of TRPV1, HSF1 and Hsp70 were examined using immunohistochemical and ELISA methods. Results: The results of this study showed that heat exposure at 45oC;70% RH for 60 minutes resulted in weight loss from day 1st to day 21st of the treatment. Peaks elevation in skin temperature, rectal temperature and heart rate were reached at day 7th, and decreased gradually from day 10th to day 21st even though the intensity of heat exposure was unchanged. This indicated the mechanism of acclimatization and thermotolerance process had occurred on the 7th day of heat treatment. There was increased in the size of the cardiomyocyte width and heart weight along with the duration of heat exposure. These results indicated the occurrence of heart hypertrophy but not accompanied by fibrosis. Molecular aspects on cardiomyocytes through Immunohistochemistry and ELISA showed TRPV1, HSF1 and Hsp70 expression as protective proteins and cardioprotectors, which tended to increase from day 1st to 7th day of treatment and decrease gradually on day 10th to day 21st. Changes in expression levels of TRPV1, HSF1 and Hsp70 coincided with changes in skin temperature, rectal temperature and heart rate. Conclusion: Heat exposure to the body induced the development of heart hypertrophy and coincided with the increased expression of TRPV1, HSF1 and Hsp70 which act as a protective protein and cardioprotector.

Abstrak :
Virus Ebola adalah salah satu penyakit paling mematikan di dunia, dengan hampir 29.000 kasus melaporkan dan membunuh 11.000 dari mereka, tetapi tidak ada perawatan atau vaksin yang dapat melawan penyakit ini secara efektif. Penyakit ini disebabkan oleh virus ebola (EBOV), anggota utama dari keluarga Filoviridae. Siklus hidup virus ini telah dioperasikan oleh beberapa mayor protein, salah satunya adalah protein HSP70, yang telah dikenal penting peran dalam transkripsi dan replikasi EBOV. Karena itu, targetkan protein HSP70 dapat menjadi solusi untuk mengobati penyakit patogen ini. Dalam penelitian ini, skrining virtual Produk alami Indonesia dilakukan sebagai inhibitor HSP70 EBOV. Molekuler simulasi docking dilakukan untuk menguji interaksi dan afinitas ligan obligasi dengan protein HSP70 EBOV; simulasi ini dilakukan dengan menggunakan MOE 2014.09 perangkat lunak. Hasil yang diperoleh menunjukkan bahwa penghambatan HSP70 berkurang secara signifikan Replikasi EBOV dengan menggunakan ligan senyawa bahan alami Indonesia. Itu nilai bioavailabilitas diperoleh sebesar 0,56. Ini menunjukkan bahwa obat tersebut dapat digunakan secara oral.

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The Ebola virus is one of the deadliest diseases in the world, with nearly 29,000 cases reporting and killing 11,000 of them, but there is no treatment or vaccine that can fight this disease effectively. This disease is caused by the Ebola virus (EBOV), a major member of the Filoviridae family. The life cycle of this virus has been operated by several major proteins, one of which is the HSP70 protein, which has been recognized for an important role in the transcription and replication of EBOV. Therefore, targeting the HSP70 protein can be a solution to treat this pathogenic disease. In this study, virtual screening of Indonesian natural products was carried out as an EBP HSP70 inhibitor. Molecular docking simulation was carried out to test the interaction and affinity of bond ligands with the EBP HSP70 protein; This simulation was carried out using MOE 2014.09 software. The results obtained showed that HSP70 inhibition was significantly reduced by EBOV replication using ligands of Indonesian natural compounds. The bioavailability value was obtained at 0.56. This shows that the drug can be used orally.

Abstrak :
This volume brings together experts in the biochemistry, cellular biology, immunology and molecular biology of molecular chaperones and protein-folding catalysts with a focus on the mechanisms of cellular trafficking of these proteins and the role of these variegated trafficking mechanisms in both human and animal health and disease.