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Abstrak :
A research on the desiccation sensitivity and cryopreservation ability of rambutan excised embryo have been conducted. Excised embryo was desiccated into target moisture content of 30%, 25%, 20%, 15%, 10%. It was found that the critical level for moisture content is 20% with 60% viability, 7.431 % per etmal growth rate and 929 µs electric conduction. The excised embryo with 20% moisture content was them soaked in 3 different protectant and stored in cryotube. The result showed that the temperature success storage was on - 10°C for 10 hours with 95.7%-100% viability. Storage at -20°C the same soaking time (10 hours) gave only 10% viability. It is concluded that excised embryo of rambutan seeds will not be successful in cryopreservation.Rambutan (Nephelium lappaceum L) is a fruit native to the Malesian region. It has many botanical relatives including the "rambutan Irian or Matoa". Based on its storage characteristic the rambutan seeds are categorized into recalsitrant seed. This seed is unable to germinate when dried and stored at low temperature. However one of the best method known to store seeds is to put them in liquid nitrogen in a dry condition. This method is called cryopreservation and it is usually done after certain chemical have been added to the seeds as a tissue protectant. Based on this evidence a research on how rambutan seed could stand cryopreservation has been conducted. Two kinds of laboratory procedure were done. The first was to investigate on how rambutan excised embryo could withstand drying. Excised embryo were desiccated into target moisture content of 30%, 25%, 20%, 15%, and 10%_ The observation focus on parameter observed were the percentage of viability, growth rate and electric conductiveness, The result show, that excised embryo of rambutan seeds were still capable to germinate on 20% of water content with 60% viability, 7.431% per etmal growth rate and 929 Its value of electric conduction. The second procedure aim to know the best protectants to be used as well as the lowest temperature that the excised embryo could withstand. The excised embryo were given three kinds of protectants, Le. sucrose, glycerol and combination of sucrose and glycerol, for 2,5,10,15 and 18 hours soaking time respectively. This lot of excised embryo was then divided into groups and each group were stared in - 10°C and -20°C respectively. Statistical analysis shows that the interaction between type of protectan and soaking time and storage at -10°C is significant for the value of p < 0.05. It was found that 10 hours soaking time of gave the best percentage of viability (with the application 100% sucrose, 96.7% glycerol, 96.7% combination of the chemical) and low value of electric conductiveness (43.33 µs, 36.00 µs and 35.67µs respectively). However the soaking time of 5, 15, and 18 hours did not give a good result, When stored at -20°C and 10 hours soaking time of the viability went down to 6-10%. Other combination of soaking time gave 0% viability. It can be then concluded that rambutan excised embryo can only be dried down to 20% moisture content without significant damage. Subsequent storage at - 20oC gave a survival level of 6-10% viability. However, a future investigation on its cryopreservation method is proposed.

Abstrak :
the aim of this research was to determine the effect of physical, chemical and physiological of several maturity stages of rambutan cultivar simacan in Wonosaro, Gunung Kidul, Yogyakarta. this research was conducted from march to may 2004 used a single factorial randomized complete design (RCD), consisted of six levels, i.e: A ( rambutan with red stages of maturity), B (rambutan with yellowness red stages maturity ) C (rambutan with yellow stages of maturity ), D ( rambutan with greenness yellow stages of maturity) , and E (Rambutan with green stages of maturity). the treatment was done in three replications. based on the research, it is better for farmers to harvest rambutan simacan once they are in red stage. in this stage the rambutan will have its sugar content and vitamin C content in its higher.

Abstrak :
Hiperurisemia adalah kondisi dimana terjadi peningkatan kadar asam urat diatas normal sehingga dapat menyebabkan penumpukan kristal asam urat di jaringan. Xantin oksidase merupakan enzim yang berperan dalam mengkatalisis oksidasi hipoxantin menjadi xantin dan menjadi asam urat. Oleh karena itu, penghambatan xantin oksidase menjadi target untuk menurunkan produksi asam urat. Penelitian ini bertujuan untuk menguji kemampuan kulit rambutan dalam menghambat xantin oksidase dan mengidentifikasi golongan kandungan kimianya. Serbuk simplisia diekstraksi dengan cara maserasi bertingkat menggunakan tiga pelarut berdasarkan tingkat kepolaran yaitu n-heksana, etil asetat dan metanol. Pengujian penghambatan aktivitas xantin oksidase dilakukan dengan metode spektrofotometri pada λ = 274,79 nm dengan kondisi pH 7,8, konsentrasi substrat xantin 0,15 mm dan suhu inkubasi 30°C. Uji penghambatan pada Alopurinol sebagai kontrol positif memiliki nilai IC50 sebesar 0,15 µg/ml. Ekstrak kulit rambutan yang memiliki daya hambat tertinggi pada enzim xantin oksidase adalah ekstrak metanol dengan nilai IC50 sebesar 3,71 µg/ml. Penapisan fitokimia pada ekstrak teraktif menunjukkan bahwa ekstrak metanol kulit rambutan mengandung flavonoid, saponin, tanin dan terpenoid.

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Hyperuricemia is a condition have higher uric acid levels that can cause a cumulation uric acid crystals in the tissues. Xanthine oxidase is an enzyme which catalyze the oxidation of hypoxanthine into xanthine and into uric acid.Therefore, the inhibition of xanthine oxidase will reduce ammount of uric acid. This research aims to determine xanthine oxidase inhibition activity and also to identify chemical constituent group of extract Rambutan (Nephelium lappaceum Linn.) skin. Rambutan skin was extracted by graded maceration using a three solvent, based on polarity the solvent are n-hexane, ethyl acetate and methanol. The test of inhibition xanthine oxidase activity was using a spectrophotometer at λ = 274.79 nm, pH 7.8, substrate concentration of xanthine 0.15 mM and an incubation temperature of 30° C. Inhibition on Allopurinol as positive control has IC50 0.15 µg/mL. The result showed that methanol extract of Rambutan skin had the highest inhibition percentage with IC50 3.71 µg/mL. Phytochemical screening showed that the most active extract methanol from rambutan skin contain flavonoids, saponins, tannins and terpenoids.