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Abstrak :
Latar Belakang: Temulawak (Curcuma xanthorrhiza Roxb.), sebagai salah satu tanaman obat unggulan Indonesia, dilaporkan memiliki efek eradikasi terhadap biofilm Candida albicans (C. albicans). Studi analisis Scanning Electron Microscopy (SEM) melaporkan penurunan densitas biofilm serta perubahan morfologi sel C. albicans yang terpapar Ekstrak Etanol Temulawak (EET). Namun bagaimana perubahan ultrastruktur sel C. albicans pada biofilm fase maturasi yang tereradikasi EET belum diketahui. Tujuan: Menganalisis gambaran ultrastruktur sel C. albicans ATCC 10231 pada biofilm fase maturasi setelah paparan EET. Metode: Biofilm C. albicans diinkubasi selama 48 jam agar mencapai fase maturasi, kemudian kelompok perlakuan dipaparkan EET dengan konsentrasi sesuai kadar eradikasi biofilm minimal (KEBM) yaitu 30%, kelompok kontrol positif diberikan paparan nystatin 100000 IU/ml, dan kelompok kontrol negatif tidak diberikan perlakuan. Transmission Electron Microscopy (TEM) dipakai untuk melihat perubahan ultrastruktur sel C. albicans pada biofilm. Hasil: Perubahan gambaran ultrastruktur sel C. albicans pada biofilm fase maturasi yang dipaparkan EET meliputi perubahan bentuk sel, perubahan ketebalan dinding sel, serta kerusakan organel. Kesimpulan: Analisis TEM menunjukkan perubahan sel Candida albicans ATCC 10231 pada sebagian sel biofilm fase maturasi yang tereradikasi EET. ......Background: Javanese turmeric (Curcuma xanthorrhiza Roxb.), as one of Indonesia’s eminent medicinal plant, had been reported for having eradication effect towards Candida albicans biofilm. SEM studies have shown that the exposure of javanese turmeric ethanol extract decreases the biofilm density and made changes in C. albicans cells morphology. However, the changes in cellular ultrastructure of C. albicans in biofilm at maturation phase which has been eradicated by javanese turmeric extract is not yet known. Objective: Analyzing the image of cellular ultrastructure of C. albicans ATCC 10231 biofilm at maturation phase after exposed to Javanese turmeric ethanol extract using Transmission Electron Microscopy. Method: C. albicans biofilm was incubated for 48 hours to achieve maturation phase, then the treatment group was exposed to 30% javanese turmeric ethanol extract according to the minimum biofilm eradication concentration, the positive control group was exposed to nystatin 100000 IU/ml, and the negative control group wasn’t exposed to anything. TEM was used to observe the cellular ultrastructure changes of the C. albicans biofilm. Result: The changes observed in the image of cellular ultrastructure of C. albicans biofilm treatment group were different cellular shape, different cell wall thicknesses, and damaged organelles. Conclusion: TEM analysis showed the changes occurred in the image of some cells in C. albicans biofilm at maturation phase which has been eradicated by javanese turmeric ethanol extract.

Abstrak :
Curcuma xanthorrhiza Roxb. adalah tanaman asli Indonesia yang memiliki kandungan xantorizol dan kurkuminoid, terutama pada bagian rimpangnya. Ekstraksi rimpang temulawak menggunakan pelarut cairan ionik (IL) dengan metode ultrasound assisted extraction (UAE). IL merupakan salah satu pelarut alternatif yang polaritasnya dapat dirancang bergantung pada kation dan anionnya dan UAE merupakan salah satu metode ekstraksi modern dengan keuntungan berupa konsumsi jumlah pelarut yang lebih rendah. Tujuan dari penelitian ini ialah mengoptimasi ekstraksi, mendapatkan kondisi optimum ekstraksi, dan membandingkan hasil ekstraksi rimpang temulawak C12MIMCl-UAE dengan maserasi-etanol 96%. Variabel bebas yang digunakan dalam kondisi optimasi ialah konsentrasi IL, waktu ekstraksi, dan rasio serbuk-pelarut. Ketiga variabel tersebut dilakukan perancangan menggunakan metode Response Surface Methodology-Box Behnken Design (RSM-BBD) dan dihasilkan 17 runs kondisi optimasi. Hasil optimum dari ke-17 runs tersebut terdapat pada run ke-5 dengan kondisi ekstraksi berupa konsentrasi IL 0,12 M, waktu ekstraksi selama 20 menit, dan rasio serbuk-pelarut 1:30 g/mL. Perolehan kadar xantorizol dan kurkuminoid terhadap serbuk pada kondisi optimum IL-UAE dengan penambahan Na2SO4 dan diklorometana ialah 43,121 mg/g dan 10,576 mg/g dengan rendemen sebesar 29,99%. Hasil tersebut lebih tinggi dibandingkan dengan perolehan kadar xantorizol dan kurkuminoid terhadap serbuk dengan metode ekstraksi maserasi-etanol 96%, yaitu 9,256 mg/g dan 2,163 mg/g dengan rendemen sebesar 8,92%. ......Curcuma xanthorrhiza Roxb. (javanese turmeric), is an Indonesia native plant that contains xanthorrhizol and curcuminoid, especially in its rhizome. Extraction of javanese turmeric rhizomes has been done using ionic liquid (IL) as a solvent by ultrasound assisted extraction (UAE) method. IL is one of the alternative solvents whose polarity can be designed according to its cation and anion and UAE is one of the modern extraction methods with the advantage of consuming a lower amount of solvent. The aim of this study is to optimize extraction, obtain optimum extraction conditions, and to compare the extraction results of javanese turmeric rhizome using C12MIMCl-UAE and maceration-96% ethanol. The all variables used optimization conditions are IL concentration, extraction time, and powder-solvent ratio. The three variables were designed using the Response Surface Methodology-Box Behnken Design (RSM-BBD) method and produced 17 runs of optimization conditions. The optimum results of the 17 runs were found in the 5th run with extraction conditions: 0.12 M of IL concentrations, 20 minutes of extraction times, and 1:30 g/mL of powder-solvent ratios. The xanthorrhizol and curcuminoid levels in powder under optimum conditions of IL-UAE with the addition of Na2SO4 and dichloromethane were 43,12 mg/g and 10,58 mg/g with 29,99% of yield. The result were higher than those of maceration-96% ethanol extraction method, which could only extract 9,26 mg/g and 2,16 mg/g of xanthorrhizol and curcuminoid content with 8,92% of yield.