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Abstrak :
Periodontitis adalah penyakit inflamasi yang disebabkan oleh faktor multifaktorial. Toll-like receptor 4 (TLR-4) dan matrix metalloproteinase-8 (MMP-8) melakukan modifikasi pada keparahan dan progresivitas penyakit periodontal dengan cara menghancurkan jaringan kolagen di epitel gingiva. Tujuan: Mendapatkan hubungan tingkat ekspresi TLR-4 dan MMP-8 dalam cairan krevikular gingiva pada periodontitis stage IV grade C. Subjek penelitian berjumlah 21 subjek dengan umur diantara 28-61 tahun diukur dengan quantitative real-time polymerase chain reaction (qRT-PCR). Uji Mann Whitney menunjukkan terdapat kenaikan tingkat ekspresi TLR-4 dan MMP-8 pada periodontitis stage IV grade C (p<0,05). Uji Spearman menunjukkan tidak terdapat hubungan antara TLR-4 dengan progresivitas periodontitis stage IV grade C, sebaliknya terdapat hubungan antara tingkat ekspresi MMP-8 dengan progresivitas periodontitis stage IV grade C (p<0,05). Kesimpulan: Terdapat hubungan antara tingkat ekspresi MMP-8 dengan progresivitas periodontitis stage IV grade C dan tidak terdapat hubungan ekspresi TLR-4 dengan progresivitas periodontitis stage IV grade C. ......Periodontitis is an inflammatory disease caused by multifactorial factors. toll-like receptor 4 (TLR-4) and matrix metalloproteinase-8 (MMP-8) modify periodontal disease severity and progression by destroying collagen tissue in gingival epithelium. Objective: Determine the expression of TLR-4 and MMP-8 in gingival crevicular fluid (GCF)in patients with periodontitis stage IV grade C. Level of expression of TLR-4 and MMP-8 from 18 subject aged 28-61 years were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Mann Whitney test of TLR-4 showed significantly higher expression level compared with control as well as MMP-8 (p<0.05). Expression of TLR-4 showed no correlation with the progression of Periodontitis stage IV grade C. Expression of MMP-8 have a moderate correlation to the progression of Periodontitis stage IV grade C. Conclusions: There are correlation between MMP-8 Expression with Periodontitis Stage IV grade C Progression, but not with TLR-4.

Abstrak :
Latar Belakang: Periodontitis merupakan salah satu penyakit kesehatan gigi dan mulut yang paling sering dijumpai sering terjadi di masyarakat. Periodontitis disebabkan oleh banyak faktor, salah satunya adalah: Penyebab penting adalah keterlibatan bakteri Aggregatibacter actinomycetemcomitans yang merupakan 'penanda' bakteri periodontitis yang memainkan peran dalam pengembangan kehilangan perlekatan jaringan periodontal, serta bakteri Fusobacterium nucleatum memiliki kemampuan untuk menggumpal di awal dan akhir kolonisasi bakteri dalam perkembangan plak sehingga bertindak sebagai jembatan bakteri. Propolis dilaporkan memiliki zat antibakteri yaitu flavonoid dan polifenol yang meningkatkan aktivitas antioksidan saliva dan menghambat penyakit periodontal. Tujuan : Menganalisis efektivitas gel propolis dalam menghambat pertumbuhan bakteri Aggregatibacter actinomycetemcomitans dan Fusobacterium nucleatum. Metode : Aggregatibacter Biofilm actinomycetemcomitans dan Fusobacterium nucleatum terkena propolis gel dengan konsentrasi 5mg/ml dan 10mg/ml kemudian diinkubasi selama 4 jam (fase adhesi), 12 jam (fase akumulasi aktif) dan 24 jam (fase pematangan) pada suhu 37°C. Persentase potensi Penghambatan pembentukan biofilm dinilai menggunakan uji MTT. Bakteri Aggregatibacter actinomycetemcomitans dan Fusobacterium nucleatum pada BHI . agar Letakkan paper disk yang telah terkena propolis gel dengan konsentrasi 5 mg/ml dan 10 mg/ml kemudian diinkubasi selama 4 jam, 6 jam, dan 8 jam pada suhu 37°C. Zona rintangan Pertumbuhan bakteri diukur dengan menggunakan penggaris. Kesimpulan: Pengaruh paparan propolis gel dalam menghambat pembentukan biofilm dan zona hambat bakteri Aggregatibacter actinomycetemcomitans dan Fusobacterium nucleatum berbeda dalam setiap durasi paparan dan variasi konsentrasi yang digunakan.
Background: Periodontitis is one of the most common dental and oral health diseases that often occur in the community. Periodontitis is caused by many factors, one of which is: An important cause is the involvement of the bacterium Aggregatibacter actinomycetemcomitans which is a 'marker' of periodontitis bacteria that plays a role in the development of periodontal tissue attachment loss, and Fusobacterium nucleatum bacteria have the ability to agglomerate at the beginning and end of bacterial colonization in plaque development so that it acts as a bacterial bridge. Propolis is reported to have antibacterial substances, namely flavonoids and polyphenols that increase salivary antioxidant activity and inhibit periodontal disease. Objective : To analyze the effectiveness of propolis gel in inhibiting the growth of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum bacteria. Methods: Aggregatibacter Biofilm actinomycetemcomitans and Fusobacterium nucleatum were exposed to propolis gel with concentrations of 5mg/ml and 10mg/ml then incubated for 4 hours (adhesion phase), 12 hours (active accumulation phase) and 24 hours (maturation phase) at 37°C. Percentage of potential inhibition of biofilm formation was assessed using the MTT assay. Bacteria Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum in BHI. agar Place the paper disk that has been exposed to propolis gel with a concentration of 5 mg/ml and 10 mg/ml then incubated for 4 hours, 6 hours, and 8 hours at 37°C. The zone of inhibition Bacterial growth was measured using a ruler. Conclusion: The effect of exposure to propolis gel in inhibiting the formation of biofilms and the inhibition zone of the bacteria Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum differs in each duration of exposure and variations in concentration used.

Abstrak :
Periodontitis is a multifactorial disease associated with genetic and environmental factors. Single-nucleotide polymorphisms (SNPs) are associated with susceptibility to common diseases such as diabetes and periodontitis. Although the oral cavity is exposed to various organisms, the conditions are well controlled by innate and acquired immune systems. Antimicrobial peptides (AMPs) play an important role in the innate immune system; however, the association of AMP-SNPs with periodontitis has not been fully elucidated. This study investigated the relationship between AMP-SNPs and periodontitis in Japanese. One hundred and five Japanese subjects were recruited, which included patients with aggressive, severe, moderate and mild periodontitis, and age-matched healthy controls. Genomic DNA was isolated from peripheral blood and genotypes of SNPs of β-defensin-1 and lactoferrin genes (DEFB1: rs1799946, rs1800972 and rs11362; and LTF: rs1126478) were investigated using the PCR-Invader assay. Protein level of AMPs in gingival crevicular fluid (GCF) was quantified by ELISA. Case–control studies revealed that the −44 CC genotype of DEFB1 (rs1800972) was associated with periodontitis (OR 2.51), particularly with severe chronic periodontitis (OR 4.15) and with combined severe and moderate chronic periodontitis (OR 4.04). No statistical differences were found in other genotypes. The β-defensin-1 concentrations in GCF were significantly lower in subjects with the −44 CC genotype of DEFB1 than in those without this genotype. No significant differences between GCF concentrations of AMPs and other genotypes were detected. The −44 CC genotype of the β-defensin-1 gene (DEFB1 rs1800972) may be associated with susceptibility to chronic periodontitis in Japanese.

Abstrak :
Latar Belakang: Diabetes mengganggu sistem kekebalan umum yang dapat mempengaruhi respon periodontium terhadap patogen. Tingkat keparahan inflamasi pada pasien periodontitis disertai diabetes melitus (PD-DM) juga dipengaruhi oleh tingkat perilaku kebersihan mulut. Penelitian ini menganalisis hubungan antara kesadaran diri terhadap kondisi periodontal (KP) dengan pengetahuan tentang periodontitis (PP) dan perilaku kebersihan mulut (POH) pada pasien PD-DM. Metode: Penelitian ini merupakan penelitian survei potong silang pada masa Pandemi Covid-19. Kuesioner tatap muka dilakukan pada kelompok PD-DM (n=29), periodontitis (n=31), dan kontrol (n=24). Reliabilitas kuesioner ditentukan dengan Cronbach. dan regresi linier berganda digunakan untuk mengkorelasikan PP dan POH dengan KP pada kelompok PD-DM, periodontitis, dan kontrol. Hasil: Uji-T parsial dan uji-F simultan pada kelompok PD-DM masing-masing p<0,05 dan p<0,001. KP atau OHB pada kelompok PD-DM berkorelasi signifikan dengan KP. Kontribusi PP (56%) lebih besar mempengaruhi KP dibandingkan POH pada kelompok PD-DM. Kesimpulan: Terdapat hubungan antara PP dan POH terhadap KP pada kelompok PD-DM. ......Objectives: Diabetes compromises the general immune system that could affect the response of the periodontium to the pathogen. The severity of inflammation in periodontitis and person with diabetes (PWD) patients was also influenced by the level of oral hygiene behaviour. This study analyzes the relationship between self-awareness of periodontal condition (SAP) to the knowledge of periodontitis (KP) and oral hygiene behaviour (OHB) in diabetic patients. Materials and Methods: This study is a cross-sectional survey design during the Covid-19 Pandemic. A face-to-face questionnaire was conducted for periodontitis with the PWD group (n=29), periodontitis group (n=31), and healthy/gingivitis patients or the control group (n=24). The reliability of the questionnaire was determined with Cronbach’s . Multiple regression linear used to correlate the KP and OHB to SAP in the PWD group. Results: The result of T-test partial and F-statistic test (simultaneous significance test) in PWD group was p < 0,05 and p < 0,001 respectively. The KP or OHB in the PWD group by each or either of their significantly correlate to SAP. The KP contribution (56%) was greater to affect SAP than OHB in the PWD group. Conclusion: There was a linear relationship between KP and OHB to SAP in the PWD group.

Abstrak :
Tubuh akan mengalami penurunan kemampuan menghadapi berbagai stimulus dan stres dari lingkungan saat lansia. Penurunan respons imun merupakan bentuk kemunduran fungsi pada lansia sehingga lansia menjadi lebih rentan terpapar patogen. Periodontitis merupakan penyakit pada jaringan periodontal yang sering terjadi pada lansia. Periodontitis terjadi ketika terdapat interaksi antara respons imun dan patogen. Penelitian ini bertujuan untuk membandingkan status periodontal dan kadar sitokin antara lansia dengan dewasa penderita periodontitis, serta menganalisis hubungan status periodontal dengan kadar sitokin. Subjek penelitian berjumlah 40 orang penderita periodontitis yang terdiri dari 20 lansia dan 20 dewasa. Pengukuran klinis status periodontal mencakup Indeks Plak dan Indeks Perdarahan Papila. Pemeriksaan sitokin mencakup sitokin pro-inflamasi Interleukin 1 Beta (IL-1β), Tumour Necrosis Factor Alpha (TNF-α), dan sitokin anti-inflamasi Interleukin 10 (IL-10) yang diambil dari cairan krevikular gingiva (CKG). Pengambilan CKG diperoleh dari gigi yang memiliki kedalaman poket 5-7 mm. Kadar sitokin IL-1β, IL-10 dan TNF-α dalam CKG diukur menggunakan tes ELISA. Rerata nilai Indeks Plak dan Indeks Perdarahan Papila pada lansia lebih tinggi dibandingkan dengan dewasa. Kadar sitokin pada lansia lebih tinggi pada lansia dibandingkan dewasa, meskipun tidak signifikan secara statistik. Terdapat korelasi yang kuat antara status periodontal dan kadar sitokin dalam CKG. Studi ini menunjukkan penuaan memengaruhi respons peradangan. ......Aging can change the ability to respond to various stimuli and stress. The decreased immune response is a form of deterioration of function in the elderly. Periodontitis is an abnormality of periodontal tissue that often occurs in the elderly. Periodontitis occurs when there is an interaction between the host immune system and the pathogen. The aim of this study is to compare periodontal status and cytokine levels between elderly and adults with periodontitis, and to analyze the relationship between periodontal status and cytokines level. This clinical study examined 40 subjects with periodontitis, consisting of 20 adult and 20 elderly. Clinical measurement of periodontal status included Plaque Index (PlI) and Papilla Bleeding Index (PBI). Cytokines examination included proinflammatory cytokine Interleukin 1 Beta (IL-1β), Tumor Necrosis Factor Alpha (TNF-α), and anti-inflammatory cytokine Interleukin 10 (IL-10) from gingival crevicular fluid (GCF). GCF collection was obtained from teeth with a probing depth of 5-7 mm. Cytokine levels of IL-1β, IL-10 and TNF-α in GCF were quantified using ELISA kits. The mean value of PI and PBI in the elderly was higher than in adults. Cytokine levels in the elderly were higher than in adults, although there was no statistical difference. There was a strong correlation between periodontal status and cytokines level in GCF. This study indicates aging affects the inflammation response.

Abstrak :
ABSTRAK
Periodontitis merupakan penyakit kronis rongga mulut yang berkontribusi menjadi bebanpenyakit kronis di dunia. Keradangan kronis yang berat pada periodontitis kronis PK akan menimbulkan respon sistemik terhadap bakteri, dan produk kerusakan periodontal.Hubungan antara PK dengan diabetes melitus tipe-2 DM tipe-2 terjadi karena infeksioral merupakan faktor predisposisi DM tipe-2, sebaliknya DM tipe-2 menjadi faktorpredisposisi PK. Adipositokin, diantaranya resistin dan adiponektin, merupakan sitokinyang berperan sebagai mediator penyakit periodontal dan DM tipe-2. Tujuan penelitianini adalah menjelaskan peran adipositokin terhadap keterkaitan antara PK dengan DMtipe-2, ditinjau dari polimorfisme gen adiponektin, kadar resistin dan adiponektin, sertapengembangan model risiko periodontitis kronis. Penelitian dilakukan terhadap 50 subjekPK non-DM usia 29-68 tahun dan 50 subjek PK dengan DM tipe-2 usia 30-73 tahun .Seluruh subjek dilakukan pemeriksaan status periodontal, status diabetes melitus, kadarresistin dan adiponektin di dalam CKG cairan krevikular gingiva maupun serum, bodymass index, serta polimorfisme gen adiponektin ADIPOQ 276G>T . Hasil uji bivariatpada subjek PK antara non-DM dengan DM tipe-2 menunjukkan terdapat perbedaanbermakna pT, kadar resistin danadiponektin CKG, kendali glikemik, serta BMI. Polimorfisme gen ADIPOQ 276G>Tmerupakan faktor risiko PK. Subjek dengan genotip GT berisiko 4,2 kali menderita PKdengan DM tipe-2 dibandingkan dengan subjek genotip GG. Model risiko PK dibentukdari faktor risiko usia, LDL, indeks kalkulus, gen adiponektin serta BMI dengan kekuatanhubungan kuat >80.

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ABSTRACT
Periodontitis is a chronic disease of the oral cavity that contributes to the burden ofchronic diseases worldwide. Severe chronic inflammation in chronic periodontitis CP will lead to systemic responses cause by bacteria and the breakdown products. Theassociation between CP with type 2 diabetes mellitus T2DM occurs because oralinfection is a predisposing factor for T2DM, whereas T2DM becomes a CP predisposingfactor. Adipocytokine induces insulin resistance, acts as a mediator of periodontal diseaseand T2DM. Resistin and adiponectin are adipocytokines. The purpose of this study wasto predict the role of adipocytokines to the relationship between CP and T2DM, in termsof adiponectin gene polymorphisms, levels of gingival crevicular fluids GCF and serumresistin and adiponectin, and the CP susceptibility risk model. Fifty subject with CP nondiabetes age range 29-68 and 50 CP subject age 30-73 with T2DM were selected. Theperiodontal status, diabetes status, BMI and the levels of GCF and serum resistin andadiponectin were assessed. Bivariate test showed significant differences p T gene polymorphism, resistin and adiponectin levels in GCF, HbA1clevels, and BMI between CP non-DM and CP T2DM subject. Gene polymorphismADIPOQ 276G>T is a risk factor for CP. The subject with GT genotype 4.2 times OR4.2 suffering from CP with DM type-2 compare to the subject with GG genotype. TheCP risk model was formed from risks factors age, LDL, calculus index, adiponectin geneand BMI with strong relation strength > 80 .

Abstrak :
ABSTRACT
Latar Belakang: Periodontitis merupakan penyakit yang disebabkan adanya akumulasi  bakteri sehingga dapat menyebabkan kerusakan tulang. Selama ini  tindakan preventif  periodontitis  banyak menggunakan terapi obat sintetis  sehingga menimbulkan berbagai efek samping. Oleh sebab itu, pemanfaatan dan penggunaan ekstrak etanol Hibiscus sabdariffa  diharapkan dapat memberikan alternatif bahan  preventif periodontitis. Tujuan: Menganalisis efek preventif Hibiscus sabdariffa terhadap periodontitis Metode: Pembuatan model periodontitis pada Mus musculus dilakukan dengan mengikatkan ligature silk thread pada gigi molar kedua, selanjutnya perlakuan diberikan dengan irigasi dengan salin steril Otsu-NS 0.9% (kontrol) dan ekstrak etanol rosela 10% (preventif) agar  terjadi penumpukan plak. Pada hari ke tujuh ligature dilepas diambil sampel selanjutnya  dianalisis dengan Image-J. Hasil: Tidak terjadi perbedaan bermakna antara kelompok perlakuan kontrol dengan ekstrak etanol rosela 10%. Kesimpulan: Ekstrak etanol kelopak bunga rosela10% tidak menunjukkan adanya efek preventif terhadap kerusakan tulang pada model periodontitis dengan Ligatur Silk Thread.

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ABSTRACT
Peridontitis is a disease that is caused by accumulation of bacteria that cause bone destruction. Studies have shown that antibiotic thus one of the most common preventive theraphy for periodontitis however there are side effects in prolonged use, recent studies shown that Hibiscus sabdariffa a well known traditional herbal medicine has a significant effect in retaining anti bacterial  behavior of cells. Therefore, by utilizing and using ethanol extract of  Hibiscus destruction hope to be an alternative way for an effective preventif theraphy. Objective: To analyze  preventive property of Hibiscus sabdariffa for periodontitis in maxillary  posterior region of  Swiss Webster Mouse. Methods: Periodontitis model was induced by ligature silk thread circumferentially on the maxillary second molar gingiva of Swiss Webster mouse using ligature silk thread. Spooling with sterlized saline solution Otsu-NS 0.9% for control and ehthanol extract rosela 10% for preventive theraphy, respectively. After the seventh day sampel was taken and analyze by image-J. Results: Overall bone loss occurred after the injection of Ethanol extract in Hibiscus sabdariffa 10% is 166,5µm 2 compare  with control that is 142µm2 on the site of the Ligature wire. Conclusion: Active anti-inflammation  properties  of ethanol extract in Hibiscus sabdariffa 10%  has not shown some preventive effect for periodontitis preventive theraphy.

Abstrak :
Polimorfisme gen VDR -1056 T/C berperan dalam metabolisme tulang dan mempengaruhi fungsi imun yang memicu terjadinya resorpsi tulang pada periodontitis. Tujuan: Untuk mengetahui perbedaan pola distribusi polimorfisme gen VDR -1056 T/C pada penyakit periodontitis dengan kelompok kontrol. Metode: Polimorfisme gen -1056 VDR dianalisis menggunakan metode PCR-RFLP dengan enzim restriksi Taq I. Hasil: Terdapat polimorfisme gen VDR -1056 T/C pada kasus dengan frekuensi genotip TC 44.5 dibandingkan dengan kelompok kontrol 55.6 . Sedangkan untuk frekuensi alel C pada kasus 44.4, dan kelompok kontrol 55.6. Kesimpulan: Distribusi polimorfisme gen VDR -1056 T/C pada penyakit periodontitis sebesar 44.5 genotip TC, 50.5 genotip TT, dan 0 genotip CC. Namun tidak terdapat perbedaan yang bermakna antara distribusi polimorfisme gen VDR -1056 T/C pada penyakit periodontitis dan kelompok kontrol p=1,0. ...... VDR 1056 T C gene polymorphism is involved in bone metabolism and affect the immune function that leads to bone resorption in periodontitis. Objective: To know the difference of distribution pattern of the gene VDR 1056 T C polymorphism in periodontitis disease with control group. Methods: The VDR 1056 T C gene polymorphism was analyzed by the PCR RFLP method with Taq I restriction enzyme digestion. Results: There are VDR 1056 T C gene polymorphism in the case with TC genotype frequency 44.55 compared with the control group 55.6 . As for the frequency of C alleles in the case 44.4 , and control group 55.6. Conclusion: The distribution of VDR 1056 T C gene polymorphism in periodontitis disease was 44.5 TC genotype, 50.5 TT genotype, and 0 CC genotype. But there was no significant difference between the distribution of VDR T C gene polymorphism in periodontitis and control group p 1.0.

Abstrak :
ABSTRACT
Pendahuluan: Periodontitis atau kerusakan tulang rahang merupakan salah satu penyakit mulut yang paling sering terjadi. Kerusakan tulang ini dapat mengganggu aktivitas manusia karena rasa tidak nyaman yang ditimbulkan. Sampai saat ini, belum ada obat kerusakan tulang yang murni terbuat dari herbal, padahal Indonesia merupakan negara tropis yang kaya akan tumbuh-tumbuhan. Tumbuhan rosella memiliki kandungan yang bisa berperan sebagai anti inflamasi sehingga ekstrak rosella sudah banyak digunakan untuk pengobatan ulser. Akan tetapi, belum ada penelitian yang menguji efektivitas rosella apabila digunakan sebagai obat kerusakan tulang. Oleh karena itu, diperlukan penelitian pada hewan coba dimulai dari tingkat mamalia terbawah yaitu Mus musculus. Tujuan: Mengetahui efektivitas ekstrak etanol kelopak bunga rosella dalam membantu proses penyembuhan kerusakan tulang. Metode: Penelitian in vivo pada enam ekor Mus musculus. Kerusakan tulang calvaria dibuat pada hari pertama dengan injeksi LPS dari bakteri E. coli. Pada hari kedua, tiga ekor Mus musculus dari kelompok kontrol diberi injeksi saline sedangkan tiga ekor Mus musculus lainnya dari kelompok perlakuan diberi injeksi ekstrak etanol kelopak bunga rosella teridentifikasi 10%. Pada hari kelima, semua Mus musculus dikorbankan dan tulang calvarianya akan diamati menggunakan Micro-CT. Hasil: Kelompok terapi yang diinjeksi dengan ekstrak rosella memiliki luas area kerusakan tulang yang lebih kecil daripada luas area kerusakan tulang pada kelompok kontrol yang diinjeksi dengan saline. Kesimpulan: Ekstrak etanol kelopak bunga rosella teridentifikasi 10% efektif dalam membantu proses penyembuhan kerusakan tulang.

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ABSTRACT
Background: Periodontitis or jaw bone damage is one the most common mouth disease. This bone damage can interfere with human activities because of the discomfort. Even though Indonesia is a tropical country that is rich in plants, there is still no pure bone medicine made from herbs. Roselle plants contain ingredients that can act as anti-inflammatory so its extract has been widely used for ulcer treatment. However, no studies have tested the efficacy of roselle when used as a medicine for bone damage. Therefore, research on experimental animals is needed starting from Mus musculus as the lowest level mammals. Objective: To analyze the efficacy of roselles ethanol extract in helping the healing process of bone damage. Methods: In vivo study on six Mus musculus. Calvarial bone damage was made on the first day by injection of LPS from E. coli bacteria. On the second day, three Mus musculus from the control group were injected by saline while the other three Mus musculus from the treatment group were injected by 10% identified roselles ethanol extract. On the fifth day, all Mus musculus were sacrificed and the calvarial bones were observed using Micro-CT. Results: The theraphy group injected by roselles ethanol extract has less bone damage area than the control group injected by saline. Conclusion: The 10% identified roselle`s ethanol extract is effective in helping the healing process of bone damage.

Abstrak :
Pendahuluan: Periodontitis disebabkan oleh ketidakseimbangan mikroorganisme pada sulkus gingiva yang menyebabkan inflamasi dan resorpsi tulang. Bakteri Porphyromonas gingivalis dianggap menjadi spesies kunci dalam patogenesis penyakit periodontitis dengan mengganggu respon imun penjamu. Ekstrak etanol kelopak bunga rosela telah terbukti memiliki khasiat antibakteri terhadap bakteri dalam rongga mulut. Oleh karena itu, dilakukan pengembangan sediaan gel ekstrak etanol kelopak bunga rosela untuk pemakaian dalam rongga mulut. Tujuan: Mengetahui efektivitas gel ekstrak etanol kelopak bunga rosela (Hibiscus sabdariffa Linn.) terhadap bakteri Porphyromonas gingivalis. Metode: Pada uji zona hambat, cakram kertas dipaparkan dengan gel ekstrak etanol kelopak bunga rosela 10%,15%, 25%, kontrol positif serta kontrol negatif dan diletakkan di atas medium Mueller-Hinton Agar yang telah diinokulasi P. gingivalis ATCC 33277. Inkubasi dilakukan selama 6 jam pada kondisi anaerob dengan suhu 37oC. Uji Total Plate Count dilakukan dengan menghitung jumlah koloni P. gingivalis yang masih hidup setelah dipaparkan dengan gel ekstrak etanol kelopak bunga rosela 10%,15%, 25%, kontrol positif dan kontrol negatif. Hasil: Gel ekstrak etanol kelopak bunga rosela konsentrasi 15% dan 25% menunjukkan adanya zona hambat terhadap bakteri Porphyromonas gingivalis dan penurunan koloni P. gingivalis yang signifikan. Kesimpulan : Gel ekstrak etanol kelopak bunga rosela memiliki efek antibakteri terhadap P. gingivalis secara in vitro. ......ntroduction: Periodontitis is caused by microorganism dysbiosis in gingival sulcus that lead to tissue inflammation and bone loss. Porphyromonas gingivalis is considered as a keystone species in the progression of periodontitis which altered host immune response and induced proinflammatory cytokine. Ethanolic roselle calyx extract has been proven as an antibacterial agent against oral pathogens. Thus, we develop ethanolic roselle calyx extract gel formulation for intraoral application to prevent periodontitis. Objective: To investigate the antibacterial activity of ethanolic roselle calyx extract gel against P. gingivalis. Methods : In the disc-diffusion test, P. gingivalis ATCC 33277 was cultivated on Mueller-Hinton Agar. Sterile paper disks were enriched with 10%, 15%, and 25% ethanolic roselle calyx extract gel, then were placed on the surface of agar and were incubated for 6 hours in anaerobic condition. In total plate count method, the viable bacteria colony were counted after exposure with 10%, 15%, and 25% ethanolic roselle calyx extract gel. Results: Ethanolic roselle calyx extract gel 15% and 25% showed an inhibition zone against P. gingivalis and significantly reduced the number of P. gingivalis colony in the total plate count test. Conclusion: Ethanolic roselle calyx extract gel have antibacterial properties against P. gingivalis.