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Abstrak :
Markisa manis (Passiflora ligularis Juss) tumbuh di dataran tinggi yang sejuk di Indonesia, salah satunya provinsi Sumatera Barat. Markisa manis memiliki aktivitas antioksidan yang kuat dan memiliki potensi dalam penghambatan tirosinase dari bagian tanamannya. Tujuan dari penelitian ini adalah menganalisis kadar flavonoid, kadar polifenol, aktivitas antioksidan (1,1-difenil-2-pikrilhidrazil (DPPH) dan Ferric Reducing Antioxidant Power (FRAP)) dan penghambatan tirosinase dari ekstrak dan fraksi bagian tanaman markisa manis, selanjutnya fraksi bagian ter-aktif diformulasikan dalam sediaan krim masker wajah. Penelitian ini menggunakan empat bagian tanaman markisa manis yaitu daun, batang, biji dan kulit buah. Proses ekstraksi dilakukan dengan pelarut etanol 70% menggunakan metode Ultrasound-Assisted Extraction (UAE). Ekstrak bagian tanaman yang menunjukkan hasil pengujian terbaik selanjutnya dilakukan fraksinasi dengan n-heksan, etil asetat dan akuades. Fraksi bagian tanaman ter-aktif dilakukan pengujian antioksidan dan aktivitas penghambatan tirosinase, kemudian diidentifikasi menggunakan Liquid Chromatograpy Mass Spectroscopy (LC-MS). Fraksi ter-aktif diperoleh dari bagian biji sebagai fraksi etil asetat biji yang diformulasikan dalam sediaan krim masker wajah. Hasil penelitian menunjukkan aktivitas antioksidan ekstrak dan fraksi yang sangat kuat hingga kuat dan memiliki potensi penghambatan tirosinase. Sedangkan krim masker wajah menunjukkan aktivitas antioksidan yang sangat kuat dan potensi penghambatan tirosinase pada F1 dan F2. Stabilitas 12 minggu menunjukkan terjadinya penurunan aktivitas antioksidan, penghambatan tirosinase dan evaluasi fisik sediaan pada F1 dan F2. ......Sweet granadilla (Passiflora ligularis Juss) grows in the cool highlands of Indonesia, one of which is the province of West Sumatera. Sweet granadilla has potent antioxidant activity and potential to inhibit the tyrosinase enzyme from plant parts. The purpose of this study was to analyze the content of flavonoid, polyphenols, antioxidant activity (1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP)) and inhibition of tyrosinase from extracts and fraction of sweet granadilla plant parts, then the most active part fraction were formulated in facial mask cream. This study used four parts of the sweet granadilla. They were leaves, stems, peels and seeds. The extraction process was carried out with 70% ethanol solvent with Ultrasound-Assisted Extraction (UAE) method. Extracts of plant parts that showed the best results then fractionated with n-hexane, ethyl acetate and distilled water. The selected fraction from plant parts was tested for antioxidant activity and inhibition of tyrosinase then identified was done by Liquid Chromatography Mass Spectroscopy (LC-MS). The most active fraction was obtained from the seeds as the ethyl acetate fraction of the seeds. This ethyl acetate fraction was formulated in a face mask cream. The results showed that the antioxidant activity of the extracts and fractions was very strong to strong and had the potential for tyrosinase inhibition. While the face mask cream showed very strong antioxidant activity and tyrosinase inhibition potential in F1 and F2. The 12-week stability showed a decrease in antioxidant activity, tyrosinase inhibition and physical evaluation of the preparation in F1 and F2.

Abstrak :
ABSTRACT
Biji markisa ungu (Passiflora edulis) diketahui mengandung polifenol yang dapat mengatasi tanda penuaan. Kandungan polifenol biji markisa ungu diekstraksi menggunakan etanol 80%. Penelitian ini bertujuan untuk memperoleh formula masker mata hidrogel dengan kestabilan dan karakteristik fisik yang baik. Masker mata hidrogel yang mengandung ekstrak biji markisa ungu 0,1% diformulasikan dengan alginat 3% dan xanthan gum 0,5%, selanjutnya dibiarkan dalam larutan kalsium klorida 0,5% selama 60 menit. Kestabilan dan karakteristik fisik masker mata hidrogel dievaluasi melalui pengamatan organoleptis, daya mengembang, konsistensi, kekuatan peregangan, dan persen elongasi. Hasil evaluasi menunjukkan masker mata hidrogel tidak mengalami perubahan organoleptis selama 12 minggu dan memiliki kemampuan menahan air yang baik. Selain itu, masker mata hidrogel memiliki kekuatan peregangan sebesar 4,0823 ± 0,6879 kgf/cm2 dan persen elongasi sebesar 200%. Masker mata hidrogel yang dihasilkan pada penelitian ini memiliki karakteristik dan stabilitas fisik yang baik sehingga diharapkan dapat digunakan sebagai sediaan perawatan kulit nutrakosmesetika.

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ABSTRACT
Purple passion fruit (Passiflora edulis) seed had known containing polyphenol which could prevent aging. Polyphenol content were extracted using 80% ethanol. The aim of this research was to obtain a hydrogel eye patch formula with good physical characteristic and stability. Hydrogel eye mask which contains 0.1% purple pasion fruit seeds extract was formulated with 3% alginate, 0.5% xanthan gum, and immersed at 0.5% calcium chloride solution for 60 minutes. Hydrogel eye mask physical characteristic and stability was evaluated by organoleptic observation, swelling index, consistency, tensile stregth, and elongation rate. The results showed that hydrogel eye mask was stable in colour and odour for 12 weeks and showed a good water holding capacity. In addition, hydrogel eye mask had tensile strength at 4.0823 ± 0.6879 kgf/cm2 and elongation rate at 200%. This research demonstrated that hydrogel eye mask formula has good physical characteristic and stability. Thus, it had potential to be used as nutracosmeceutical skin care product.