Defisiensi vitamin D rentan terjadi pada tenaga kesehatan dan berakibat pada gangguan sintesis cathelicidin, peptida antimikrobial dengan efek proteksi terhadap virus. Studi terdahulu menunjukkan adanya korelasi positif antara 25-OH-D dengan cathelicidin, sementara data terkait pada populasi obesitas masih terbatas. Penelitian dengan desain potong lintang dilakukan di Rumah Sakit rujukan pasien COVID-19 di Jakarta dan Depok. Consecutive sampling dan randomisasi dilakukan untuk memperoleh sampel. Asupan makronutrien dan vitamin D dinilai menggunakan Food recall 24 jam dan semi kuantitatif Food Frequency Questionnaire (SQ-FFQ). Kadar 25-OH-D dan cathelicidin serum dianalisa dengan metode Chemiluminescence Immunoassay (CLIA) dan Enzyme Linked Immunosorbentassay (ELISA). Uji Mann Whitney dan Kruskal Wallis dilakukan untuk menilai perbedaan rerata kadar cathelicidin, sementara korelasi 25-OH-D dan cathelicidin serum dinilai dengan regresi linear setelah penyesuaian terhadap Indeks Massa Tubuh (IMT). 80 subjek usia 22 hingga 47 tahun dianalisa, dengan 70% subjek memiliki status gizi obesitas dan 30% berat badan lebih. 93.7% subjek belum mencukupi kebutuhan asupan harian Vitamin D dengan median asupan Vitamin D 2.8 µg per hari. Median kadar 25-OH-D dan cathelicidin subjek 14.3 ng/ml dan 211.6 ng/ml. 85% subjek tergolong defisiensi vitamin D dan subjek dengan obesitas II memiliki kadar cathelicidin yang lebih tinggi. Tidak didapatkan korelasi antara kadar 25-OH-D dengan cathelicidin serum pada subjek tenaga kesehatan dengan berat badan lebih dan obesitas (p 0.942 ð-0.077 95% CI -2.182-2.029). Hasil penelitian ini membutuhkan analisa lebih lanjut mengingat peningkatan kadar cathelicidin dapat dipengaruhi oleh variabel perancu sehingga efek protektif dari cathelicidin belum dapat disimpulkan.
Vitamin D deficiency is prevalent among healthcare workers, resulting in impairment of cathelicidin, an antimicrobial peptide with antiviral properties. Former studies show a positive correlation between 25-OH-D and cathelicidin, yet data on the obese population is still scarce. We conducted a cross-sectional study in the COVID-19 referral hospitals in Jakarta and Depok. Samples were collected using consecutive sampling followed by randomization. A repeated 24-hour food recall and a semi-quantitative food frequency questionnaire (SQ-FFQ) were used to estimate intake. The Enzyme-Linked Immunosorbent Assay (ELISA) and Chemiluminescence Immunoassay (CLIA) were used to measure serum cathelicidin and 25-OH-D. Mann Whitney and Kruskal Wallis analyses were done to assess the mean difference of cathelicidin, and linear regression adjusted for body mass index was done to assess the correlation between 25-OH-D and cathelicidin. 80 subjects aged 22 to 47 years were included, where 70% of the subjects were categorized as obese and 30% were overweight. 93.7% of the subjects did not meet their daily intake of vitamin D requirements, with a median intake of vitamin D of 2.8 µg daily. The subject’s median serum of 25-OH-D and cathelicidin were 14.3 ng/ml and 211.6 ng/ml, respectively. 85% of the subjects were classified as vitamin D deficient, and subjects with class II obesity had significantly higher levels of cathelicidin. Serum 25-OH-D and cathelicidin did not correlate in overweight and obese healthcare workers (p 0.942 ð-0.077 95% CI -2.182-2.029). Further research is essential to better understand the findings of this study since the protective effects of cathelicidin cannot be determined because confounding factors may cause cathelicidin levels to rise.
Latar Belakang: Prevalensi berat badan lebih dan obesitas pada pekerja usia dewasa mengalami peningkatan dari tahun ke tahun seiring dengan perubahan gaya hidup yang terjadi. Kedua hal yang mencerminkan komposisi tubuh yang buruk ini merupakan faktor risiko dari berbagai penyakit kronik. Sebaliknya, daya tahan kardiorespirasi yang baik dapat memberikan berbagai manfaat kesehatan. Tujuan: Mengetahui pengaruh perubahan daya tahan kardiorespirasi terhadap komposisi tubuh pada pekerja duduk. Metode: Penelitian ini menggunakan metode potong lintang dengan sumber data sekunder. Sejumlah 82 subjek penelitian yang merupakan pekerja duduk di Jakarta tahun 2018, dibagi menjadi kelompok uji dan kontrol yang masing-masing terdiri dari 41 subjek. Kelompok uji mendapatkan intervensi berupa latihan fisik berbasis tempat kerja selama 12 minggu. Analisis data dilakukan dengan menggunakan uji korelasi Pearson, Spearman, dan uji T-berpasangan. Hasil: Didapatkan peningkatan rerata nilai Indeks Massa Tubuh sebesar 0,14 kg/m2, peningkatan rerata presentase lemak sebesar 0,56%, penurunan rerata ukuran lingkar pinggang sebesar 2,56 cm, dan peningkatan rerata nilai prediksi daya tahan kardiorespirasi sebesar 1,27 mL/kg/menit pada subjek yang menjalani program latihan fisik berbasis tempat kerja selama 12 minggu, walaupun tidak ditemukan hubungan yang signifikan antara perubahan pada nilai prediksi daya tahan kardiorespirasi terhadap ketiga komponen komposisi tubuh tersebut. Simpulan: Peningkatan daya tahan kardiorespirasi dengan program latihan fisik berbasis tempat kerja selama 12 minggu memberikan perbaikan secara klinis pada komposisi tubuh pekerja duduk, meskipun tidak menghasilkan perubahan yang signifikan secara statistik. Diperlukan penelitian lanjutan dengan memerhatikan dugaan faktor-faktor yang memengaruhi hasil tersebut.
......Background: The prevalence of overweight and obesity in adults has increased in recent years in line with lifesyle changes that occur. These two things that reflect poor body composition are risk factors for various chronic disease. Conversely, good cardiorespiratory fitness can provide health benefits. Objective: This research was done to determine the effect of changes in cardiorespirator fitness on body composition in sitting workers. Methods: This study uses a cross-sectional method with a secondary data sources. A total of 82 research subject were sitting workers in Jakarta in 2018, which were divided into test and control groups, each grup consisting of 41 subjects. The test group received an intervention in the form of workplace based physical exercise for 12 weeks. Data analysis was performed using the Pearson and Spearman correlation test, and paired T-test. Results: There was an increase in the mean value of Body Mass Index by 0,14 kg/m2, an increase in the mean value of percentage of body fat by 0,56 percent, a decrease in the mean value of waist circumference by 2,56 cm, and an increase in the mean predicted value of cardiorespiratory fitness by 1,27 mL/kg/minutes in subjects undergoing a workplace based physical exercise program for 12 weeks, although no significant relationship was found between changes in the predicted value of cardiorespiratory endurance on the three components of body composition. Conclusion: Increased cardiorespiratory endurance with a workplace-based physical exercise program for 12 weeks provided a clinical improvement in sitting workers body composition, although it did not produce statistically significant changes . Further research is needed by considering other factor that may influence this result.