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Ditemukan 2 dokumen yang sesuai dengan query
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Antonius Suwanto
"Tempeh is sliceable soybean-cake fermented by Rhizopus oligosporus. Various bacteria were detected in tempeh employing cultivation technique with limited information about their origin or sources. The present study aimed to examine the source/s of bacterial community in tempeh by combining metagenomics analysis and cultivation technique. Samples were obtained from a number of steps in tempeh production employing two-times boiling of soybean (WJB tempeh production). All samples were plated on Enterobacteriaceae and Lactic Acid Bacteria medium. Total DNA were extracted directly from tempeh for metagenomics analysis, employing High-Throughput Sequencing (HTS) and cloned 16S rRNA genes. Firmicutes and Proteobacteria were the predominant and second dominant bacteria existed in fresh tempeh (FT) obtained by metagenomics analysis. In contrast, cultivation technique showed that Proteobacteria was the predominant phylum, suggested that most of the Firmicutes were not culturable. FT was dominated by Lactobacillus and Acetobacter. Both FT and soaking water (SW) were dominated by same species of Lactobacillus, i.e. L. delbreuckii and L. mucosae, indicated that SW was probably the source of bacterial community established in the final product of fermentation. Predominant bacteria in starter culture (SC), Acinetobacter, was not detected in FT, indicating that bacteria in SC might not play significant role in bacterial community development in FT."
Bogor: Seameo Biotrop, 2021
634.6 BIO 28:1 (2021)
Artikel Jurnal  Universitas Indonesia Library
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Farrah Nabilla Irfan
"Identifikasi komunitas mikrobiota menjadi salah satu pendekatan penting untuk meningkatkan kualitas hidup dan kesehatan gajah sumatra dalam konservasi exsitu. Penelitian ini memfokuskan pada analisis mikrobiota usus berdasarkan empat sampel feses (anak betina/jantan, induk betina/jantan) menggunakan Nextgeneration Sequencing dan analisis metagenomik. Analisis metagenomik yang mencangkup alpha dan beta diversity memberikan pengetahuan terkait komposisi mikrobiota dari kelimpahan, kemerataan dan perbedaan diantara keempat sampel. Analisis alpha diversity menunjukkan variasi mikrobiota yang signifikan, dengan gajah jantan dewasa memiliki keanekaragaman dan kelimpahan mikrobiota tertinggi, sementara gajah betina dewasa menunjukkan keanekaragaman yang paling rendah. Analisis beta diversity menunjukkan gajah anak jantan memiliki perbedaan signifikan dari kedua gajah dewasa karena perbedaan umur yang signifikan. Komunitas mikrobiota didominasi oleh filum Bacillota (40-70%) dan Bacteroidota (20-40%), dengan Clostridia (~90%) menjadi kelas paling melimpah dalam Bacillota, sementara Sphingobacteriia (~50%) dan Bacteroidia (~50%) kelas dominan dalam Bacteroidota. Temuan ini mengindikasikan adanya variasi yang signifikan dalam komposisi mikrobiota usus di antara sampel, yang mungkin dipengaruhi oleh faktor seperti jenis makanan dan usia. Pemahaman lebih lanjut tentang hubungan ini penting untuk merancang strategi konservasi yang efektif guna meningkatkan kesehatan dan kesejahteraan gajah sumatra di lingkungan konservasi.

Identification of the microbiota community is an important approach to improve the quality of life and health of Sumatran elephants in ex-situ conservation contexts. This research focuses on analyzing gut microbiota based on four fecal samples (female/male child, female/male adults) using Next-generation Sequencing and metagenomic analysis. Alpha diversity analysis shows significant microbiota variation, with adult males having the highest diversity and abundance, while adult females exhibit lower diversity. Beta diversity analysis indicates moderate differences between female and male juveniles, with male juveniles significantly differing from both adult elephants. The prokaryotic community is dominated by Bacillota (40-70%) and Bacteroidota (20-40%), with Clostridia (~90%) being the most abundant group within Bacillota, and Sphingobacteriia (~50%) and Bacteroidia (~50%) dominant within Bacteroidota. These findings indicate significant variations in gut microbiota composition among samples, likely influenced by factors such as diet and age. Further understanding of these relationships is crucial for designing effective conservation strategies to enhance the health and well-being of Sumatran elephants in conservation environments."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2024
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UI - Skripsi Membership  Universitas Indonesia Library