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Abstrak :
Pendahuluan: Malignant pleural mesothelioma merupakan suatu keganasan yang bersifat agresif dan memiliki prognosis yang buruk. Hingga saat ini belum ditemukan suatu pilihan ter- api bermakna yang dapat memberikan respon baik. Dari beberapa penelitian dikatakan bahwa terdapat hubungan antara suatu proses epithelial mesenchymal transition (EMT) dengan kondisi prognosis yang buruk pada keganasan. Lysine specific demethylase-1 (LSD1) merupakan suatu enzim yang terlibat dalam proses EMT dan terdapat pada berbagai jenis kanker. Sehingga LSD1 dinilai memiliki peran penting dalam proses EMT. Tujuan: Untuk mengetahui peran LSD1 pada malignant pleural mesothelioma Metode: Studi ini mengobservasi overexpress SNAIL pada ACC-MESO 4 dengan gambaran karakteristik epithelial. Ekspresi protein-protein yang berhubungan dengan EMT dan LSD1 di- periksa menggunakan western blot assay. Uji proliferasi dan migrasi sel dievaluasi dengan wound healling assay Hasil: Peningkatan ekspresi SNAIL menginduksi perubahan bentuk sel pada ACC-MESO-4 menjadi spindle-like shape sesuai proses EMT. Aktivasi SNAIL dengan ekspresi yang sangat tinggi berpengaruh terhadap proses penurunan ekspresi E-cadherin serta peningkatan ekspresi Fibronectin dan Vimentin. Peningkatan ekspresi SNAIL juga mengakibatkan sel ACC-MESO 4 menjadi agresif sehingga meningkatkan migrasinya. Penekanan ekspresi LSD1 dapat meng- hambat terjadinya proses EMT pada MPM. Kesimpulan: LSD1 memiliki peran yang penting terhadap proses EMT dan penekanannya dapat menghambat terjadinya proses EMT. ......Introduction: Malignant pleural mesothelioma (MPM) has an aggressive characteristic and poor prognosis. To date, there were no significant therapy which have good response to MPM. Several studies said that there were correlation between epithelial mesenchymal transition (EMT) process and poor prognosis in cancer. Lysine-specific demethylase-1 (LSD1), an en- zyme which involved in EMT process and overexpressed in many types of cancers, could be a potential target to attenuate EMT process. This study demonstrated the role of LSD1 during EMT process in MPM. Aim: To know the role of LSD1 in malignant pleural mesothelioma Methods: This study observed an overexpressed SNAIL transfection in ACC-MESO 4 that has epithelial characteristic. All protein expression related EMT marker and LSD1 were examined by western blot. We evaluated migration profile with wound healing assay test. Results: The overexpression of SNAIL have changed the cell morphology to become spindle shape-like, consistent with an EMT process. After the overexpression of SNAIL, e-cadherin expression level was down-regulated while fibronectin and vimentin were up-regulated. This EMT phenotype also increased the migration rate. Finally, when we suppressed LSD1 with LSD1 inhibitor, the EMT process could be attenuated. Conclusion: This study showed that LSD1 has an important role during EMT process and in- hibition of LSD1 could attenuated EMT process.

Abstrak :
ABSTRAK
Stress oksidatif merupakan dasar dari berbagai penyakit degeneratif dan kanker, termasuk asbestosis dan mesotelioma. Kedua penyakit tersebut terjadi akibat terinhalasinya serat asbes dalam jangka waktu lama dan jumlah pajanan yang tinggi. Penelitian potong lintang di Sekretariat Buruh Karawang bulan Oktober 2014 dilakukan untuk menilai korelasi asupan dan kadar vitamin C, E dengan kadar isoprostan. Lima puluh dua subjek yang bekerja di pabrik asbes selesai mengikuti seluruh protokol penelitian. Hasil menunjukkan bahwa asupan vitamin C dan kadar vitamin E berkorelasi negatif dengan kadar isoprostan, sedangkan asupan vitamin E dan kadar vitamin C berkorelasi positif dengan kadar isoprostan pekerja pabrik asbes. Korelasi tersebut secara statistik tidak bermakna. Penelitian lanjut diperlukan untuk menilai kadar isoprostan secara series, sehingga bila ada peningkatan yang signifikan dapat segera dikethui.

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ABSTRACT
Oxidative stress is the base of various degenerative diseases and cancers, including asbestosis and mesothelioma. Both of them occur due to prolonged inhalation of asbestos fibers and high level of exposure. A cross-sectional study at a labor secretariat in October 2014 was performed to assess the correlations between intakes and levels of vitamin C and vitamin E and isoprostane level. Fifty two subjects working at an asbestos factory finished the study. The result showed that vitamin C intake and vitamin E level were negatively correlated with isoprostane level. Meanwhile, vitamin E intake and vitamin C level were positively correlated with isoprostane level in asbestos factory workers. These correlations were statistically insignificant. Asbestos factory workers should be educated to increase their intakes of vitamin C and vitamin E.