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"ABSTRAKInfeksi HIV dapat terjadi melalui hubungan seksual, pemakaian alat-alat kedokteran yang tercemar, pemakaian komponen darah yang telah terpapar HIV ataupun secara transplasental dari ibu yang terinfeksi HIV.Pada anak, umumnya infeksi ini terjadi melalui transfusi komponen darah, terutama mereka yang secara terus menerus memerlukannya karena menderita penyakit tertentu seperti hemofilia atau thalassemia.Tujuan penelitian ini ialah untuk mengetahui proporsi infeksi HIV pada anak yang telah menerima transfusi komponen darah berulang, khususnya penderita hemofilia dan thalassemia.Selama periode satu tahun (Sept 92- Sept 93) telah diteliti serum dari 40 penderita hemofilia dan 40 penderita thalassemia mayor, terhadap infeksi HIV dengan metoda ELISA.Hasil penelitian menunjukkan bahwa tidak satupun dari para penderita tersebut yang telah terpapar HIV.

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ABSTRACTHuman immunodeficiency virus infection can occur via direct bloodstream inoculation from blood or blood products or infected needles, through sexual contact and transplacentally from an infected mother.In children, the major mode of transmission of HIV is from transfusion of blood or blood products, especially those who require repeated and regular transfusion because of their specific illness.The purpose of this study is to investigate the proportion of HIV infection in children with hemophilia and thalassemia who had received repeated blood or blood product transfusions.During a period of 12 months (Sept. 92 - Sept. 93) sera from 40 children with hemophilia and 40 children with thalassemia were tested against HIV infection using ELISA method.Result of this study showing no HIV infection could be detected in all children."

"Di Indonesia saat ini masalah PMS-HIV/AIDS merupakan hal yang patut diwaspadai dan diantisipasi lebih dini, mengingat prevalensinya meningkat terus-menerus dari tahun ke tahun. Posisi Indonesia yang sangat strategis ini dianggap rentan terhadap terjadinya endemi penyakit HIV/AIDS. Salah satu faktor yang berperan dalam penanggulangan PMS-HIV/AIDS adalah perilaku pencarian pengobatan. Beberapa hasil penelitian sebelumnya menunjukkan rendahnya perilaku pencarian pengobatan pada kelompok pria.Penelitian ini bertujuan untuk mengetahui faktor-faktor yang berhubungan dengan perilaku pencarian pengobatan pada pria dengan PMS-HIV/AIDS di Jakarta, Surabaya dan Manado, dengan menggunakan data sekunder dari Behavioral Surveillance Survey PMS- HIV/AIDS tahun 2000 yang dilakukan oleh Pusat Penelitian Kesehatan Universitas Indonesia didukung USAID. Rancangan penelitian ini adalah studi potong lintang (Cross sectional Study), dengan pengolahan data menggunakan analisis regresi logistik ganda.Hasil penelitian ini menunjukkan proporsi perilaku pencarian pengobatan kurang baik pada responden sebesar 75,3%. Tidak ada hubungan yang bermakna antara umur, status perkawinan, sumber informasi dengan perilaku pencarian pengobatan. Variabel pengetahuan dan pendidikan berhubungan bermakna dengan perilaku pencarian pengobatan dan tidak ada interaksi antara pendidikan dan pengetahuan. Responden yang berpengetahuan kurang berpeluang melakukan pencarian pengobatan kurang baik 1,8 kali (95% CI: 1,1724-2,6442) dibandingkan dengan responden yang berpengetahuan baik setelah dikontrol variabel pendidikan. Sedangkan berdasarkan latar belakang pendidikan formal yang telah ditamatkan, ternyata responden yang berpendidikan rendah berpeluang melakukan pencarian pengobatan kurang baik 1,7 kali (95% CI: 1,0236- 2,5805) dibandingkan responden yang berpendidikan tinggi setelah dikontrol variabel pengetahuan.Dari penelitian ini disarankan perlunya dilakukan penelitian selanjutnya untuk menggali lebih dalam alasan respoden yang tidak melakukan pencarian pengobatan. Perlu penyuluhan yang lebih intensif, konsisten dan berkelanjutan dengan cara menyebarluaskan informasi dengan memanfaatkan media yang diminati. Bagi pemerintah perlu menjadikan program tetap dan pengalokasian dana tidak hanya untuk pengobatan juga untuk pelayanan kesehatan dan konseling.Daftar pustaka: 58 (1979-2002)

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The Factors Related to Health Seeking Behaviour of Men with STD - HIV/AIDS in Jakarta, Surabaya and Manado (Secondary Data Analysis, USAID, 2000)Nowdays, STD ? HIV/AIDS in Indonesia; are issues we have to anticipate and alert earlier, considering the prevalence increases from year to year. The strategic position of Indonesia is considered susceptible to the pandemy of HIV/AIDS. One of the involving factor HIV/AIDS is health seeking behavior. Previous studies showed the low of health seeking behavior in men's group.This study is aimed to discover the factors related to health seeking behavior of men with STD ? HIV/AIDS in Jakarta, Surabaya, and Manado by using secondary data analysis from behavioral surveillance survey of STD ? HIV/AIDS in 2000 that was conducted by the center of health research, University of Indonesia by USAID support. This study using cross sectional design, and data processing with multiple logistic regression analysis.The result of this study shows that the proportion of men health seeking behaviour is poor (75,3% respondents). There are no relationship between age, marital status, information sources with the health seeking behavior. The variables of education and knowledge related with health seeking behavior and there is no interaction between education and knowledge. The respondents with low knowledge have the possibilities of poor health seeking behavior 1,8 times (95% CI 1,I725 --2,6442) compared with those who have good knowledge after being controlled by the education variable. Meanwhile, based on the formal educational background that the respondents got through, those who have low education have the possibilities of health seeking behavior 1,7 times (95% 1,00236 - 2,5805) compared with those who have higher education after being controlled by knowledge variable.This study recommends the need of further research to discover more detailed explanations why respondents do not seek for health care, and need more intensive, consistent and continually health education by spreading out information by using the interested media. It is necessary for government to make the prevention program of STD ? HIV/AIDS as an annual program and the fund allocation is not only for medication but also for the health and counseling services."

"HIV exposure among health workers is still quite rare, including in Indonesia. Nevertheless, with the increase in new HIV cases due to intravenous drug abuse, there should be more health workers caring for HIV cases. To avoid exposure, universal precaution has to be implemented. If exposure occurs, the HIV stale of the patient, as the source of body fluid should be determined, while the exposed health care worker needs to undergo counseling. Anti retroviral agents should be administered prior to 36 hours following exposure. Sero-conversion monitoring must be performed during exposure, also 3 months, 6 months, and 12 months following exposure- There have been 9 cases of HIV exposure due to accidents among health workers reported to the Working Group on AIDS (Kelompok Studi Khusus - Pokdiksus AIDS) Faculty of Medicine of the University of Indonesia - Cipto Mangunkusumo General Central National Hospital. Six of them received AZT prophylactic treatment, while the remaining 3 chose not to use any prophylactic treatment. After six months following exposure, all anti HIV test were negativeThe number of HIV cases in the last two years has shown a tremendous increase. AT the end of February 2002, the Department of Health recorded 2/50 cases of HIV/AIDS in Indonesia.1 As new cases increase among intravenous drug abuse, the number of HIV cases is estimated to increase further in the future, bearing in mind that experts have estimated that number of drug abusers in Indonesia have reached 2 million people. Those infected with HIV, especially those already in the AIDS stage, often require hospitalization for treatment of opportunistic infections. Thus, health workers have to prepare themselves to face the increasing problem of HIV infection.To avoid contagion of HIV, Hepatitis B, and Hepatitis C thai may reside in the patient's body fluids to another person, the Center for Disease Control (CDC> recommends universal precaution. This guideline from C DC should be continuously distributed to allow health workers to continue to work with a feeling of security.Nevertheless, there is still the possbility of accidents among health workers at work in the form of needle prick or direct exposure to body fluids from an HIV-infected individual."

"Jumlah pengidap virus HIV di Indonesia terus meningkat dari jenis penularannya, lebih banyak melalui cairan genital daripada plasma darah. Deteksi HIV diperlukan untuk pencegahan dan pengobatan. Teknik yang lazim digunakan adalah amplifikasi DNA dengan metode PCR. Penelitian ini bertujuan menerapkan teknik amplifikasi DNA metode LAMP yang baru-baru ini dikembangkan sebagai ganti PCR karena lebih spesifik, sensitif dan efisien. LAMP menggunakan pasangan primer yang unik, sepasang primer forward dan sepasang backward yang masing-maing terdiri dari primer panjang untuk polimerisasi DNA dem sepasang primer pendek untuk melepas rantai baru DNA sehingga reaksi bisa dilakukan pada suhu tetap. Reaksi LAMP menggunakan enzim Bst DNA polymerase pada suhu 65°C dilakukanterhadap isolat genom RNA HW sudah dikonfirmasi keberadaannya dengan metode PCR."

"Human Immunodeficiency Virus type-I (HIV-1) merupakan penyebab sindroma penurunan sistem imun tubuh yang disebut dengan Acquired Immunodeficiency Syndrome (AIDS). Infeksi HIV-I di dunia dan Indonesia cenderung meningkat. Pemeriksaan yang cepat dan spesifik diperlukan untuk mencegah penyebaran infeksi HIV-I. Berbagai teknik telah dikembangkan untuk deteksi infeksi HIV-I. Pada penelitian ini dikembangkan pemeriksaan RT-PCR HIV-1 Mikrobiologi FKUI (in-house RT-PCR) untuk mendapatkan uji alternatif deteksi HIV-1. Sebanyak 46 plasma dan serum kelompok berperilaku risiko tinggi yang berkunjung ke klinik VCT . RSUP Sanglah Denpasar, telah diperiksa dalam penelitian ini. Serum diperiksa dengan 3 kit rapid test yang berbeda yaitu DetermineTM HIV-1/2 (Abbott), ImmunoCombR HIV 1 & 2 BiSpot (Organics), dan SerodieR HIV-1/2 (Fujirebio Inc.). Plasma diuji dengan pemeriksaan RTPCR generasi I menggunakan primer spesifik terhadap daerah gag dan RT-PCR generasi 2 menggunakan primer spesifik terhadap daerah protease dari genom HIV-1. Hasil rapid test menunjukkan dari 46 sampel, sebanyak 26 serum (56,5%) reaktif dan 20 serum (43,5%) non-reaktif. Tingkat sensitivitas, spesifisitas, nilai duga positif, dan nilai duga negatif RT-PCR generasi 1 secara berturut-turut adalah 80,8%, 95%, 95,5%, dan 79,2%, sedangkan rasio kemungkinan positif dan negatif adalah 16,2, dan 0,2. Pemeriksaan RTPCR generasi 2 menunjukkan tingkat sensitivitas 65,4%, spesifisitas 90%, nilai duga positif 89,5%, nilai duga negatif 66,7%, rasio kemungkinan positif 6,5, dan rasio kemungkinan negatif 0,4. Teknik RT-PCR yang menggunakan primer tersebut dapat mendeteksi HIV pada semua stadium klinis WHO pada kelompok ini. Sensitivitas dan spesifisitas RT-PCR generasi 1 lebih baik daripada RT-PCR generasi 2, tetapi, masih lebih rendah daripada baku emas, Secara keseluruhan, RT-PCR pada penelitian ini belum dapat direkomendasikan sebagai uji altematif baik uji skrining maupun uji konfirmasi dalam mendeteksi infeksi HIV-1.

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Human Immunodeficiency Virus type 1 (HIV-1) can cause decrease of immune response which is called Acquired Immunodeficiency Syndrome (AIDS). HIV-l infection in the world and Indonesia tends to increase. Many techniques were developed to detect HIV-1 infection. A specific and rapid diagnosis is needed to prevent transmission of HIV-1 infection. In this study, we performed RT-PCR HIV-1 Microbiology FKUI (in-house RT-PCR) as an alternative test to detect HIV-1. Forty six plasmas and serums from high risk behavior group who visited VCT Clinic Sanglah General Hospital, Denpasar were used in this study. Serums were tested with 3 different rapid test kits i.e. Determine ° IIIV-112 (Abbott), immunoComb HIV I & 2 BiSpot (Orgenics), and Serodia ' HIV-112 (Fujirebio Inc.). Plasmas were tested with I generation RT-PCR which used specific primers to gag region in HIV-1 genome and specific primers to protease region in IIIV-1 genome for 2nd generation RT-PCR. Results of rapid test demonstrated 26 serums (56.5%) were reactive and 20 serums (43.5%) were non-reactive. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 1st generation RT-PCR was 80.8%, 95%, 95.5%, 79.2%, whereas positive likelihood ratio (LR +) and negative likelihood ratio (LR -) was 16.2, and 0.2, respectively. The 2"d generation RT-PCR showed sensitivity, specificity, PPV, NPV, LR (+), and LR (-) was 65.4%, 90%, 89.5%, 66.7%, 6.5, and 0.4, respectively. These in-house RT-PCR could detect HIV-1 in all WHO clinical staging in this group. This study showed that lsi generation RT-PCR gives better results than 2"d generation RT-PCR. But still inferior than rapid test to detect HIV-1 infection. Overall, RT-PCR in this study has not been recommended yet as an alternative test to detect HIV-I infection."

"Acquired Immunodeficiency Syndrome disebabkan oleh infeksi Human Immunodeficiency Virus (HIV) terhadap sel-sel T CD4+, limfosit, dan makrofag. Sebanyak 90.7% penderita HIV di Indonesia terinfeksi oleh HIV-1 subtipe CRF01_AE. Protein Gag P7 yang dikode oleh gen gag p7 dan terdapat di dalam genom HIV-1 merupakan protein yang dapat digunakan sebagai obat antiretrovirus dan kandidat antigen untuk sistem diagnostik. Penelitian bertujuan untuk memperoleh fragmen DNA gag p7 (nukleokapsid) HIV-1 yang berukuran 210 pb. Fragmen gen gag p7 diperoleh dari hasil Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) dengan menggunakan primer forward AE_p7F dan primer reverse AE_p7R. Cetakan DNA (template) berasal dari RNA virus yang diambil dari serum pasien penderita HIV-1 di Indonesia. Hasil visualisasi pada gel poliakrilamida 10% menunjukkan bahwa fragmen gen gag p7 (nukleokapsid) berhasil teramplifikasi dengan ukuran 210 pb.

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Acquired immunodeficiency syndrome caused by human immunodeficiency virus infections to the CD4+ cells, lymphocyte, and macrophage. More than 90.7 % HIV patient in Indonesia were infected by HIV-1 subtype CRF01_AE. The Gag P7 protein coded by gag p7 gene which found in HIV genome was known as protein in which could be used for antiretroviral drugs (ARV) and diagnosis of HIV-1 infections. The purpose of research is to get DNA fragment of gag p7 (nucleocapsid) gene of HIV-1 which has length 210 bp. Fragment of gag p7 gene could be gotten from the process of reverse transcriptase polymerase chain reaction (RT-PCR) in which use primer forward AE_p7F and primer reverse AE_p7R. The templates is RNA virus that taken from patient?s serum of HIV-1 in Indonesia. The electrophoresis visualization shown that fragment of gag p7 (nucleocapsid) gene was success amplified in which has length 210 bp."

"Indonesian National Committee for human immunodeficiency virus (HIV) and acquired immune deficiency syndrome(AIDS) has reported the significant increase of HIV infected individual in Indonesia. A sensitive accurate diagnosticsare urgently needed to prevent the dissemination of HIV and also to provide a suitable therapy. For this reason, we havedeveloped HIV diagnostic method based on PCR to elucidate this problem. For this research, samples were collectedfrom hospitals and Indonesian Red Cross that consist of samples possesing HIV serological test positive andindeterminate. Ribonucleic Acid (RNA) were isolated from blood plasma. These RNA then were amplified afterReverse transcriptase reaction by using primers which have been designed using env (C2V3C3), Long TerminalRepeats (LTR) (U3) and Capsid gag (p24) as target regions. The obtained results shown 26/34 (76.5%) positive in LTR,10/33 (36.4%) positive in Env and 11/33 (33.3%) positive in p24. These results showed that LTR primers detect HIVmore than other primers. It suggests that LTR could be used as detection target as complement of env and p24 Theseresults need to be explored further by using sequencing method."

"Wanita pelacur adalah salah satu kelompok resiko tinggi untuk tertular lnfeksi Menular Seksual termasuk HIV/AIDS. Hal ini mengingat perilaku seksual yang tidak aman dengan berganti pasangan. Mereka menjaja seks di jalanan sehingga dianggap mengganggu ketertiban umum kemudian ditertibkan oleh aparat (digaruk) untuk dikirim ke panti rehabilitasi sosial. Rehabilitasi tidak selalu berjalan efektif karena terlihat setelah keluar dari panti mereka kembali ke jalan. Hal ini menjadi tantangan bagi pemerintah untuk melakukan optimalisasi peran panti sosial. Tujuan penelitian: mendapatkan gambaran tentang proscs rehabilitasi sosial yang dilakukan di kedua panti, gambaran tentang persepsi dan tingkat pcngctahuan tentang HIWAIDS, serta proses rehabilitasi sosial yang sedang dijalaninya. Metodologiz penelitian ini dilakukan studi deskriptif dengan teknik wawancara, observasi, dan studi kepustakaan, sedangkan studi kualitatif dengan cara wawancara mendalam yang dilakukan di Panti Rehabilitasi Sosial Marga Rahayu dan Mulya Jaya Jakarta sejak April-Juni 2008. Hasil: Gambaran karakteristik penghuni dengan rentang usia 16-49 tahun. Tingkat pengetahuan tentang HIV masih rendah. Pada umumnya menikah dan berasal dari Jawa Barat, mobilisasi 26,6 persen, pernah dibina lebih dari 2 kali 63 persen, persepsi tentang rehabilitasi sosial mereka mengetahui tentang kegiatan panti, tentang peratunan dan sanksi, (sanksi yang dilakukan banyak yang tidak tertulis) sebagian besar merasa senang dengan keterampilan, 72,3 persen berpozensi kembali lurun ke jalan. Hal ini menggambarkan efektifitas peran panti untuk teljadinya perubahan perilaku untuk tidak kembali menjadi pelacur. Saran: Bagi Penghuni hendaknya menngkatkan kesadaran pentingnya pencegahan untuk tidak tertular dan menularkan penyakit kepada orang lain akibat perilaku seksual yang tidak aman, dan meningkatkan keterampilan hidup (LW Skill) untuk dimanfaatkan agar tidak kembali ke jalan.Bagi panti perlu mengajarkan IW skill yang berorientasi pada kebutuhan individu dan upaya promotif tentang HIV/AIDS khususnya umumnya tentang kesehatan rcproduksi di panti jangka panjang dcngan meningkatkan kerjasama Iintas sektoral dalam rangka penyaluran penghuni setelah selesai menjalani rehabiiitasi sosial. Untuk akademisi hendaknya melakukan Studi kualitatif mengapa mereka kembali kejalan.

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The prostitute is one of the groups that have the highest risk to be infected by Sexually Transmitted Infection including HIV/AIDS (Human lmmunodejiciemy Virus/ Acquired Immune Deficiency Syndrome) unsafe sexual behavior by changing partner. They try to offer sexual relation on the street and it bothers public order. If it is happened, they are usually arrested by the social oflicers and are sent to the social rehabilitation center. The rehabilitation does not always run effectively. We can see after they followed the rehabilitation program, they go back to the street. This problem becomes a challenge for the govemment to do the maximum part of social center/house.

The aim of the research : To get the description about the process ofthe social rehabilitation which is done in both of the houses, the description about perception and thc knowicdgc of HIV / AIDS, also social rehabilitation process that is running.

Methodology: This research is done in description study through interview technique, observation and literature study. Qualitative study through depth interview is done in both of Social Rehabilitation Center Marga Rahayu and Mulya Jaya Jakarta since April-June 2008.

Result: inhabitant in that place are between I6 - 49 years old. Their knowledge of HIV is not maximum. Most of them are married and came fiom West Java, mobilization 26, 6 percent, has rehabilitated more than twice are 63 percent. Their perception about social rehabilitation are the activities, the rules, and punishment of the house (most of the punishments were unwritten). Most of them are happy because they can get some skills hom the social center. And 72,3 percent is potential to go back to the street. Those things describe the effectively of the social center in order to change the prostitute’s behavior and try to make them not go back to thc street to become a prostitute again.

Suggestion: The writers suggest the inhabitant to do the safe-sex to make them healthy and are not infected by HIV. And for the For the house, needs to teach life skill which has orientation in individual need and promotion effort especially about HIV/AIDS, and generally about reproduction healthy for long term by increasing cooperation among the sectors. The house also has to think where the prostitute must go afier following the program in social rehabilitation. And for the researcher they can do some researches why the ex-inhabitants of the social control go back to the street."

"Meningkatnya jumlah kasus HIV/AIDS telah masuk ke dalam lingkup keluarga, dimana salah satu penyebabnya akibat kurangnya pengetahuan dan melemahnya ketahanan keluarga. Penelitian ini bertujuan untuk mengetahui sejauh mana pengaruh penyuluhan terhadap pengetahuan, sikap, dan ketahanan keluarga pada ibu rumah tangga dalam mencegah HIV/AIDS di Pekanbaru. Disain penelitian adalah kuasi eksperimen rancangan one group pretest posttest. Penelitian dilakukan di Pekanbaru bulan Maret-Mei 2013 dengan menyebar angket pada 139 ibu rumah tangga. Hasil penelitian menunjukkan ada peningkatan 39,2% yang bermakna pada pengetahuan sesudah penyuluhan (p value 0,001), peningkatan 10,9% yang bermakna pada sikap sesudah penyuluhan (p value 0,001), serta peningkatan 1,25% yang bermakna pada ketahanan keluarga sesudah penyuluhan (p value 0,002).

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The growing number of cases of HIV/AIDS has entered into the scope of family, where one of the cause is due to a lack of knowledge and the weakening of family resilience. This study aims to know how far the influence of education on knowledge, attitudes, and family resilience in housewives in preventing HIV/AIDS in Pekanbaru. The research design is quasi experiment one group pretest posttest. Research was conducted in Pekanbaru March-May 2013 with spread questionnaires on 139 housewives. The results showed there was increase significant 39.2% in knowledge after education (p value 0.001), increase significant 10.9% in attitude after education (p value 0.001), and increase significant 1.25% in family resilience after education (p value 0.002)."

"Kejadian AIDS (Acquired Immunodeficiency Syndrome) yang disebabkan Human Immunodeficiency Virus tipe 1 (HIV-1) terus meningkat setiap tahunnya. Pencegahan penularan virus HIV-1 masih sulit karena belum ada vaksin yang telah ditemukan untuk mencegah penularan atau transmisi virus ini. Selain itu, faktor lainnya adalah jarangnya diagnostik yang tersedia untuk awal infeksi, serta variasi genetik virus HIV-1 yang meningkat dengan cepat. Penelitian bertujuan untuk mengembangkan virus dengan menggunakan klona galur sel T CD4 yaitu CEM-GFP dengan virus HIV-1 CRF01_AE untuk mendapatkan virus dengan sifat genetik yang relatif homogen dan sifat virus yang sama. Ekspresi virus dalam sel target dimonitor melalui induksi green fluorescent protein yang akan diekspresikan oleh CEM-GFP ketika sel ini terinfeksi oleh virus HIV-1. Infeksi dilakukan dengan dua metode yaitu direct cell to cell transmission dan cell-free virus infection, hasil infeksi kedua metode ini dibandingkan fluoresensinya dengan mikroskop fluoresensi dan pengukuran ekspresi GFP dengan sitometer.Setelah 7 hari kultur, pengamatan dengan mikroskop fluoresensi menunjukkan bahwa sel terinfeksi dengan metode direct cell to cell transmission lebih banyak dibandingkan dengan cell-free virus infection. Pengukuran ekspresi GFP dengan sitometer pun menunjukkan hal serupa dimana ekspresi GFP sel terinfeksi dengan metode direct cell to cell transmission lebih banyak dibanding dengan cell-free virus infection. Untuk melihat apakah virus berhasil dikeluarkan dari sel terinfeksi dilakukan dengan metode Polymerase Chain Reaction. Hasil menunjukkan bahwa virus telah berhasil terdeteksi pada supernatan kultur sel CEM-GFP terinfeksi virus HIV-1.

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The incidents of AIDS (Acquired Immunodeficiency Syndrome) that caused by Human Immunodeficiency Virus type 1 (HIV-1) are increasing every year. The prevention of HIV transmission is still difficult to be done because HIV vaccine has not been found yet. Besides, another factor in HIV therapy is the rare early diagnostic available and the genetic variation of HIV-1 virus that increased rapidly. This study was aimed for propagating virus by using CEM-GFP clones, the derivative of CD4 T cells infected with CRF01_AE for obtaining virus with relatively homogen genetic variation and possessing the same characteristic. The virus expression in the target cell was observed by the induction of green fluorescent protein expressed by CEM-GFP when this cell was infected by HIV-Virus. The infection was held by two methods, cell-to-cell transmission and cellfree virus infection, the fluorescent of infected cell of this two methods was compared with fluorescent microscope and GFP expression assay with cytometer.

Within 7 days of culture, observation with fluorescent microscope showed that the infected cells of direct cell-to-cell transmission method was higher than the cellfree virus infection. GFP expression assay also showed the same result. The GFP expression of infected cells with direct cell-to-cell transmission was higher than cell-free virus infection. To investigate whether the virus was released from the infected cells, Polymerase Chain Reaction, were applied in this study. The result showed that the cell-free virus can be detected in culture supernatant of CEMGFP cells infected with HIV-1."