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Abstrak :
ABSTRAK
Telah dilakukan penelitian untuk mengetahui pengaruh pemberian fortifikan NaFeEDTA dalam tepung tempe dan susu kedelai terhadap kadar natrium plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague Dawley. Sebanyak 25 ekor tikus dibagi ke dalam 5 kelompok: kelompok kontrol 1 (KK1) yang diberi CMC 0,5%, kelompok kontrol 2 (KK2) yang diberi tepung tempe atau susu kedelai, dan tiga kelompok perlakuan (KP1, KP2, KP3) yang diberi tepung tempe atau susu kedelai dengan fortifikan NaFeEDTA dosis 1,35 mg Fe/ kgBB; 2,7 mg Fe/ kgBB; 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Penentuan kadar natrium plasma dengan alat AES (Atomic Emission Spectroscopy). Hasil uji Anava satu arah (P > 0,05) menunjukkan tidak ada pengaruh nyata pemberian fortifikan NaFeEDTA dalam tepung tempe dan susu kedelai terhadap kadar natrium antar kelompok perlakuan. Kadar natrium plasma pada T21 dengan bahan uji tepung tempe dan susu kedelai tetap berada pada rentang normal antara 0,456 mg/ml -- 0,586 mg/ml.

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ABSTRACT
The effect of NaFeEDTA fortificant inserted in tempeh flour and soy milk intake on plasma sodium concentration in male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. Twenty five rats were divided into five groups: control group 1 (KK1) was administered with CMC 0.5%, control group 2 (KK2) was administered with tempeh flour or soy milk; three treatment groups (KP1, KP2, KP3) were administered with tempeh flour or soy milk added with fortificant NaFeEDTA 1.35 mg Fe/kgBw; 2.7 mg Fe/kgBw; 5.4 mg Fe/kgBw consecutive for 21 days. Plasma sodium concentration was measured by AES (Atomic Emission Spectroscopy). One way Anova test (P > 0.05) showed there is no significant effect of fortificant NaFeEDTA inserted in tempeh flour and soy milk intake on plasma sodium concentration in all treatment groups. Plasma sodium concentration on T21 which was administered with tempeh flour and soy milk remains in normal range between 0.456 mg/ml ? 0.586 mg/ml.

Abstrak :
ABSTRAK
Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan Fe Fumarat dalam tepung tempe terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi larutan CMC 0,5%; KK 2 yang diberi CMC 0,5% dan suspensi tepung tempe tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi CMC 0,5% dan tepung tempe dengan fortifikan Fe Fumarat dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah pencekokan hari ke-21. Darah ditentukan kadar zat besinya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji Anava satu arah (P < 0,05) menunjukkan perbedaan nyata pemberian fortifikan Fe Fumarat dalam tepung tempe terhadap kadar zat besi antar kelompok perlakuan. Peningkatan kadar zat besi tertinggi terjadi pada KP 1 yaitu sebesar 13,21% terhadap KK 1 dan 11,48% terhadap KK 2.

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ABSTRACT
The effect of Fe fumarate fortificant addition in tempeh flour on plasma iron concentration of male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. By using Complete Random Design (CRD), twenty five rats were divided into five groups, consist of normal control group (KK 1) which was administered with CMC 0.5%, treatment control group (KK 2) which was administered with CMC 0.5% and tempeh flour non fortificant, and three treatment groups which were administered with tempeh flour added with Fe Fumarate fortificant 1.35 mg Fe/KgBW (KP 1); 2.7 mg Fe/KgBW (KP 2); and 5.4 mg Fe/KgBW (KP 3). All of the five groups were treated within 21 consecutive days. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANOVA test ( P < 0.05) showed significant effect of Fe Fumarate fortificant addition in tempeh flour intake on plasma iron concentration in all treatment groups. The highest increase of plasma iron concentration was detected on KP 1 which is 13.21% to KK 1 and 11.48% to KK 2.