Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Latar Belakang: Enterococcus faecalis merupakan faktor etiologi utama pada lesi periapeks yang persisten dan mampu bertahan dari preparasi kemomekanis pada saluran akar. Oleh karena itu, untuk menghilangkan mikroba yang terisa dalam saluran akar, harus dilakukan pengisian saluran akar secara tiga dimensi dengan siler yang bersifat antimikroba. Siler kalsium hidroksida dan mineral trioxide aggregate bersifat biokompatibel dan keduanya melepaskan ion hidroksil yang dapat menciptakan suasana basa sehingga memberikan daya antimikroba. Tujuan penelitian ini adalah menganalisis perbedaan daya antimikroba siler mineral trioxide aggregate dan kalsium hidroksida terhadap E.faecalis pada waktu segera, 1 hari dan 7 hari setelah pengadukan siler. Metode: Daya antimikroba siler MTA dan Ca(OH)2 terhadap E.faecalis (ATCC 29212) diperiksa dengan direct contact test (DCT). Masing-masing siler saluran akar disiapkan dan dikontakkan langsung dengan E.faecalis pada waktu segera, 1 hari dan 7 hari setelah pengadukan siler, kemudian diberi medium BHI broth. Suspensi mikroba dituang pada plat agar dan diinkubasi selama 24 jam, setelah itu dihitung jumlah koloni mikroba yang tumbuh (CFU/ml). Analisis data dengan uji one way Anova dan Post Hoc. Hasil: Siler MTA pada waktu 1 hari dan 7 hari setelah pengadukan siler dapat mengurangi jumlah koloni mikroba paling baik, tidak berbeda bermakna dengan siler Ca(OH)2 pada waktu 1 hari setelah pengadukan siler. Kesimpulan: Siler MTA memiliki daya antimikroba terhadap E.faecalis yang lebih konstan dibandingkan siler Ca(OH)2. Sedangkan, siler Ca(OH)2 memiliki daya antimikroba yang menurun setelah 1 hari pengadukan siler. Daya antimikroba paling tinggi terhadap E.faecalis dihasilkan oleh siler MTA pada waktu 1 hari dan 7 hari setelah pengadukan siler serta siler Ca(OH)2 pada waktu 1 hari setelah pengadukan siler. ......Background: Enterococcus faecalis plays a significant role in the etiology of persistent periapical lesion and can survive from chemomechanical preparation of the root canal. Therefore, three-dimensional obturation of the root canal with sealers that have antimicrobial activity must be performed to completely eliminate microorganisms from the root canal. Calcium hydroxide and mineral trioxide aggregate sealers are biocompatible and able to release hydroxyl ion thus creating alkaline environment that is responsible for their antimicrobial properties. The aim of this present study was to evaluate the antimicrobial activity of mineral trioxide aggregate and calcium hydroxide sealer on E.faecalis after different periods of contact time (fresh, 1 day, 7 day after sealer preparation). Methods: Antimicrobial activity of MTA and Ca(OH)2 sealer on E.faecalis (ATCC 29212) was assessed by direct contact test (DCT). Each sealer was prepared and directly exposed to E.faecalis immediately, 1 day and 7 day after sealer preparation, and BHI broth medium was added. The microbial suspension was poured in the blood agar plates and incubated for 24 hours. The colonies grown on the plates were counted (CFU/ml). The data were analyzed with one way Anova and Post Hoc test. Results: MTA sealer on 1 and 7 day after sealer preparation was greatest at diminishing microbial colonies with no significant difference with Ca(OH)2 sealer on 1 day after sealer preparation. Conclusion: MTA sealer had constant antimicrobial activity on E.faecalis compared to Ca(OH)2 sealer. On the other hand, Ca(OH)2 sealer possessed decreasing antimicrobial activity overtime. Greatest antimicrobial activity on E.faecalis was provided by MTA sealer on 1 and 7 day after sealer preparation and Ca(OH)2 sealer on 1 day after sealer preparation.

Abstrak :
Latar Belakang: Perawatan endodontik bertujuan untuk menghilangkan infeksi mikroba yang terlibat pada sistem saluran akar. Enterococcus faecalis merupakan spesies yang terlibat dalam infeksi endodontik. E. faecalis memproduksi enzim gelatinase yang dapat membantu untuk menempel pada permukaan dentin. Propolis dikenal kaya akan bahan aktif sehingga berpotensi menjadi bahan alam untuk menunjang perawatan endodontik. Tujuan: Menetapkan efektivitas propolis 0.2%, 0.8%, dan 1.2% jika dibandingkan dengan NaOCl 5.25% serta pengaruhnya terhadap ekspresi gen gelE pada biofilm E. faecalis dan aktivitas gelatinase. Metode: Eksperimen biofilm ditumbuhkan pada 96­-well plate dan diinkubasi dalam keadaan microaerofilik selama 14, 48, dan 72 jam dan selanjutnya dilakukan uji ekspresi gen gelE dengan RT-qPCR serta uji gelatinase dilakukan pada BHI agar yang dicampurkan dengan 3mg susu skim yang dibentuk sumuran setelahnya diinkubasi selama 3, 9, dan 24 jam Hasil: Pada hasil penelitian didapatkan hasil secara kualitatif pengamatan mikroskopik adanya efektivitas propolis terhadap E. faecalis seiring dengan bertambahnya konsentrasi pada masa inkubasi 24 dan 48 jam. Dari hasil uji statistik Kruskall wallis dengan menggunakan GraphPad, ekspresi mRNA gelE dipengaruhi oleh propolis jika dibandingkan dengan kontrol negatif , hasil uji statistik didapatkan hasil yang signifikan (p < 0.05). Berdasarkan hasil dari uji gelatinase didapatkan diameter zona bening propolis 0.2%, 0.8%, dan 1.2% lebih kecil jika dibandingkan dengan kontrol negatif dan akuades secara berurutan, akan tetapi lebih besar jika dibandingkan dengan NaOCl 5.25% selaku kontrol positif. Kesimpulan: Propolis berpotensi menjadi bahan medikasi dan irigasi pada perawatan endodontik, selanjutnya perlu dilakukan penelitian lebih lanjut dengan bakteri multispesies dan konsentrasi propolis yang lebih tinggi. ......Background: Endodontic treatment aims to eliminate the microbial infection involved in the root canal system. Enterococcus faecalis is a species involved in endodontic infections. E. faecalis produces gelatinase enzymes that can help to adhere to the dentin surface. Propolis is known to be rich in active ingredients so that it has the potential to be a natural ingredient to support endodontic treatment. Objective: To determine the effectiveness of propolis 0.2%, 0.8%, and 1.2% when compared to 5.25% NaOCl and their effect on the expression of the gelE gene in E. faecalis biofilm and gelatinase activity. Methods: Biofilm were grown on a 96-well plate and incubated under microaerophilic conditions for 14, 48, and 72 hours and then tested for gelE gene expression using RT-qPCR and gelatinase test was performed on BHI agar mixed with 3mg of skim milk formed The wells were then incubated for 3, 9, and 24 hours. Results: The results obtained qualitatively microscopic observations of the effectiveness of propolis against E. faecalis along with increasing concentrations during the incubation period of 24 and 48 hours. From the results of the Kruskall Wallis statistical test using GraphPad, the expression of gelE mRNA was affected by propolis when compared to the negative control, the statistical test results obtained significant results (p < 0.05). Based on the results of the gelatinase test, the diameter of the clear zone of propolis 0.2%, 0.8%, and 1.2% was smaller than the negative control and distilled water respectively, but larger than the 5.25% NaOCl as the positive control. Conclusion: Propolis has the potential to be a medicinal and irrigation material in endodontic treatment, further research needs to be done with multispecies bacteria and higher concentrations of propolis

Abstrak :
Enterococcus faecalis adalah bakteri yang paling dominan pada kasus infeksi pasca perawatan endodontik. Telah diteliti aktivitas fenotipnya (kemampuan membentuk biofilm dan Gel E serta viabilitasnya terhadap NaOCL dan khlorheksidin) dan profil genotipnya. Ternyata tidak ada perbedaan aktivitas Gel E E. faecalis saluran akar dan saliva pasien pra dan pasca perawatan. Dalam membentuk biofilm, kemampuan E. faecalis saluran akar pra-perawatan lebih kuat daripada pasca-perawatan, sedangkan kemampuan E. faecalis saliva tidak berbeda. Viabilitas E. faecalis menurun jika kadar NaOCl dan khlorheksidin meningkat; 30 menit adalah waktu inkubasi efektif. Terdapat keragaman profil genotip antara E. faecalis saliva dan saluran akar, pra dan pasca perawatan. ......Enterococcus faecalis is strong dominance bacteria in post treatment endodontic disease. Its phenotype activity (its ability to produce biofilms and Gel E also its viability to NaOCL and chlorexidine) and its genotype profile has been observed. It turns out that there?s no difference in E. faecalis Gel E activity of root canal and saliva from pre- nor post-endodontic treated patients. In producing biofilms, the ability of E. faecalis pre-endodontic treated root canal was stronger than the postendodontic treated root canal, while the ability of E. faecalis from saliva have no difference. The viability of E. faecalis decline if the concentration of NaOCl and chlorexidine were increasing; the effective incubation time was 30 minutes. There?s various genotype profile between E. faecalis of saliva and root canal either pre- or post-endodontic treatment.

Abstrak :
Latar Belakang : E. faecalis merupakan penyebab infeksi persisten pasca perawatan saluran akar. Menghilangkan seluruh bakteri dari saluran akar masih menjadi masalah dalam perawatan endodontik. Penggunaan larutan irigasi dengan penambahan laser dioda sebagai perangkat tambahan adalah upaya untuk eliminasi tersebut. Tujuan: menganalisis dan membandingkan daya anti bakteri laser dioda sebagai perangkat tambahan irigasi dengan larutan irigasi NaOCl 2,5 ,CHX 2 terhadap biofilm E. faecalis isolat klinis. Metode : menghitung jumlah koloni CFU biofilm E. faecalis isolat klinis pasca pemaparan bahan uii. Hasil : laser dioda memiliki daya anti bakteri terhadap biofilm E. faecalis isolat klinis terlihat pada kelompok kontrol salin. Terdapat perbedaan bermakna p ......Background: E. faecalis is often found in persistent intra radicular infection after endodontic failure treatment. Eliminating all bacteria in the root canal remains a problem in root canal management. Using irrigant with laser diode as adjunct is to eliminate the bacteria. Aim: To analyze and compare the effectivity of diode laser, 2 chlorhexidine and 2,5 natrium hypochlorite against biofilm Of E. faecalis. Method: score the antibacterial effects of the irrigant and diode laser with colony form unit CFU. Result: Diode laser has antibacterial effectivity against E. faecalis biofilm. There is significant difference p 0,05 between irrigant with diode laser as an adjunct.

Abstrak :
Latar belakang : E.faecalis merupakan bakteri yang mendominasi pada infeksi saluran akar persisten yang memiliki virulensi 1000 kali lebih kuat dalam bentuk biofilm dibandingkan planktonik. Penggunaan larutan irigasi herbal dipertimbangkan dengan tujuan meminimalkan efek samping namun memiliki efektivitas yang sama dibandingkan larutan irigasi kimia. Tujuan : Untuk menganalisis kemampuan kayu secang dalam mengeliminasi biofilm E.faecalis. Metode : Biofilm E.faecalis dibagi menjadi enam kelompok yaitu kelompok kayu secang konsentrasi 625 g/ml, 1.250 g/ml, 2.500 g/ml, 5.000 g/ml, CHX 2 dan kelompok biofilm tanpa perlakuan. Hasil : Ditemui bahwa nilai rerata koloni biofilm diantara empat konsentrasi yang diuji, konsentrasi 625 g/ml memiliki efektivitas antibakteri yang sama dengan CHX , sedangkan efektivitas antibakteri konsentrasi 5.000 g/ml merupakan yang terendah dibandingkan CHX 2 . Kesimpulan : Kayu secang mempunyai efek antibakteri terhadap biofilm E.faecalis dan efektivitasnya sama dengan CHX 2 .Kata kunci : E. faecalis; biofilm; kayu secang. ......Background E.faecalis is the dominant bacteria in persistent root canal infections that have 1000 times stronger virulence in biofilms than planktonic. The use of herbal irrigation solutions is considered with the aim of minimizing side effects but having the same effectiveness as compared to chemical irrigation solutions. Objective To analyze the ability of secang heartwood in eliminating E.faecalis biofilm. Methods Biofilm E.faecalis divided into six groups, in secang heartwood concentration groups of 625 g ml, 1.250 g ml, 2.500 g ml, 5.000 g ml, CHX 2 and biofilm group without treatment. Results It was found that the mean value of the biofilm colony among the four concentrations, the concentration of 625 g ml had the same antibacterial effectiveness as CHX , while the antibacterial effectiveness of 5.000 g ml concentration was the lowest compared to CHX 2 . Conclusion Secang heartwood has antibacterial effect on E.faecalis biofilm and its effectiveness is equal to CHX 2 .Keywords Enterococcus faecalis biofilm secang heartwood

Abstrak :
Penelitian ini dilakukan untuk menguji daya antibakteri bahan irigasi nisin 10%, klorheksidin 2%, natrium hipoklorit 2,5% terhadap pertumbuhan biofilm E. faecalis secara in vitro. Bakteri E. faecalis ATCC 29212 dibiakkan pada media agar BHI kemudian diinkubasi 24 jam pada suhu 370C. Bakteri diinokulasi pada membran filter selulosa nitrat selama 72 jam agar terbentuk biofilm. Dilakukan uji kontak langsung antara bahan uji dan biofilm selama 10 menit. Dilakukan kuantifikasi DNA bakteri yang hidup melalui penambahan PMA pada Real time PCR. Hasilnya nisin 10% mempunyai daya antibakteri terhadap biofilm E.faecalis, namun tidak sebanding dengan klorheksidin 2% dan natrium hipoklorit 2,5%. ......The aim of this study was to evaluate antibacterial efficacy of 10% nisin, 2% chlorhexidine, 2.5% sodium hypochlorite against E.faecalis biofilm in vitro. Petri dishes containing BHI agar were seeded with E. faecalis ATCC 29212, incubated overnight at 370C. Celullose nitrate filter membrane was inoculated with E.faecalis for 72 hours to grown a biofilm. Direct contact test was performed between the test solutions and biofilm for 10 minutes. DNA quantification was performed using Real time PCR with PMA additive to count the lived cell. It was concluded that 10% nisin possessed antibacterial effect against E.faecalis biofilm, but not comparable with 2% chlorhexidine and 2.5% sodium hypochlorite.

Abstrak :
Latar Belakang : Enterococcus faecalis merupakan bakteri yang mampu membentuk biofilm dan banyak ditemukan pada kasus kegagalan perawatan saluran akar. Tujuan : Melihat daya antibakteri kitosan dan klorheksidin terhadap E. faecalis dalam biofilm. Metode : Deteksi dan kuantifikasi E. faecalis dalam biofilm yang hidup pasca pemaparan bahan uji, dengan real time PCR. Hasil : Terdapat perbedaan jumlah bakteri yang signifikan antara kedua kelompok bahan uji terhadap kontrol (p ≤ 0,05), tetapi tidak terdapat perbedaan bermakna antara kelompok kitosan dan klorheksidin. Kesimpulan : Daya antibakteri kitosan 2% terhadap biofilm E. faecalis sebanding dengan klorheksidin 2%. ......Background : Enterococcus faecalis has an ability to form biofilms and become a predominant bacteria that plays a major role in the etiology of persistent lesions after root canal treatment. Aim : To analyze the efficacy of chitosan and chlorhexidine against E. faecalis in biofilms. Methods : Detection and quantification of E. faecalis DNA that survive and live after immersing the biofilm in antibacterial solution, with real time PCR. Result : Statistically there is significant difference of living E. faecalis between chitosan and control and between 2% chlorhexidine and control (p ≤0,05). But there is no significant different between chitosan and chlorhexidine (p>0,05). Conclusion : Antibacterial effectivity of chitosan is equal to chlorhexidine against E. faecalis in biofilm.

Abstrak :
Latar Belakang: E.faecalis isolat klinis merupakan bakteri yang menyebabkan lesi periapeks persisten. Bioceramic dan Mineral Trioxide Aggregate merupakan siler yang mempunyai sifat anti bakteri. Tujuan: Untuk menganalisis efek anti bakteri siler Bioceramic dan MTA terhadap E.faecalis isolat klinis pada waktu 2 menit segera , 4 jam initial setting , 1 dan 7 hari setelah pengadukan siler. Metode: Efek anti bakteri siler Bioceramic dan MTA diperiksa dengan direct contact test. Masing-masing siler dikontakkan langsung dengan E.faecalis isolat klinis 2 menit, 4 jam, 1 dan 7 hari setelah pengadukan siler. Suspensi ini dioleskan pada medium agar dan diinkubasi 24 jam untuk melihat koloni bakteri yang tumbuh CFU/ml. Hasil: Terdapat perbedaan bermakna antara siler Bioceramic dan MTA pada waktu 7 hari, antara siler Bioceramic pada waktu 2 dan 4 jam serta waktu 4 jam dan 7 hari setelah pengadukan siler. Kesimpulan: siler Bioceramic dan MTA mempunyai efek anti bakteri yang baik terhadap E.faecalis pada saat segera, initial setting, dan 1 hari setelah pengadukan siler, pada waktu 7 hari setelah pengadukan, siler MTA yang paling baik. Siler MTA mempunyai efek anti bakteri yang konstan sampai 7 hari Keywords : Entereococcus faecalis isolat klinis, Siler Bioceramic dan Mineral Trioxide Aggregate MTA ......Background: E.faecalis isolate clinic is a kind of bacteria that cause persistent periapical lesion. Bioceramic and Mineral Trioxide Aggregate are sealers that having antibacterial properties. Aim: To analyze antibacterial effect of Bioceramic and MTA sealers against E.faecalis isolate clinic at 2 minutes fresh , 4 hours initial setting , 1 day and 7 day after mixed the sealers. Methods: Antibacterial effect of Bioceramic and MTA sealers was assessed by direct test contact. Each sealer was contacted with E.faecalis isolate clinic at 2 minutes, 4 hours, 1 day and 7 days after mixed the sealers. This suspension was swab in agar medium and incubated for 24 hours. The colony in agar plates is counted with colony forming unit CFU . Result: The significant differences was shown by Bioceramic and MTA at 7 days, between Bioceramic at 2 minutes fresh and 4 hours initial setting also at 4 hours initial setting and 7 days after mixed the sealer. Conclusion: Both of Bioceramic and MTA sealers have a good antibacterial effect at fresh, initial setting and 1 hour after mixed the sealer but at 7 days, MTA was the greater. MTA sealer has a constant antibacterial effect until 7 days.

Abstrak :
Latar Belakang: Penggunaan instrumen endodontik steril diperlukan untuk keberhasilan perawatan saluran akar. Desain dan bentuk instrumen endodontik menjadi tantangan dalam proses sterilisasi. Instrumen yang kini sering digunakan adalah instrumen putar NiTi, dengan salah satu desain terbaru adalah instrumen putar NiTi off-centered, yang bisa berupa variable taper maupun regressive taper. Bakteri resisten yang perlu diwaspadai dalam perawatan saluran akar adalah Enterococcus faecalis. Beberapa tahapan sterilisasi sudah pernah diajukan, namuan tidak spesifik pada instrumen putar NiTi off-centered yang terkontaminasi bakteri Enterococcus faecalis. Perlu siasat dan langkah-langkah khusus untuk mendapatkan instrumen putar NiTi off-centered yang steril dari bakteri Entercoccus faecalis. Tujuan: Membandingkan jumlah bakteri Enterococcus faecalis pada instrumen putar NiTi off-centered variable taper dan regressive taper setelah melalui dua metode sterilisasi yang berbeda. Metode: Delapan kelompok uji yang terdiri dari empat kelompok instrumen putar NiTi off-centered variable taper dan empat kelompok instrumen putar NiTi off-centered regressive taper. Masing-masing dari keempat kelompok tersebut dibagi menjadi kelompok kontrol negatif, kelompok kontrol positif, kelompok sterilisasi Parashos dan kelompok sterilisasi Linsuwanont. Selain kelompok negatif, instrumen-instrumen dari kelompok lainnya digunakan untuk mempreparasi saluran akar gigi premolar yang sudah dikontaminasi bakteri Enterococcus faecalis. Kedelapan kelompok kemudian diuji kultur menggunakan agar kromogenik untuk melihat keberadaan bakteri Enterococcus faecalis dan menghitung koloni yang terbentuk. Hasil: Pada kelompok kontrol negatif, tidak ditemukan adanya bakteri Enterococcus faecalis. Kelompok positif menunjukkan keberadaan bakteri Enterococcus faecalis dengan jumlah besar. Kelompok Parashos dan Linsuwanont menunjukkan jumlah bakteri Enterococcus faecalis yang beragam, dengan kelompok Parashos memberikan hasil jumlah bakteri yang lebih besar daripada kelompok Linsuwanont. beragam.terbanyak dan tersedikit Kesimpulan: Metode sterilisasi Linsuwanont adalah metode paling efektif untuk mensterilisasi bakteri Enterococcus faecalis pada instrumen putar NiTi off-centered regressive taper. Metode sterilisasi Parashos tidak efektif untuk mensterilisasi bakteri Enterococcus faecalis pada instrumen putar NiTi off-centered variable taper maupun regressive taper. Kata kunci: sterilisasi, intrumen putar NiTi, off-centered, Enterococcus faecalis, CFU ......Backrgound: The use of sterile endodontic instruments is necessary to achieve successful root canal treatment. The design and shape of endodontic instruments poses challenges in their sterilization process. The current most widely used endodontic instrument are NiTi rotary files, and one of the newest design results in off-centered NiTi rotary files, which can either have a variable taper or a regressive taper. Resistant bacteria that must be noticed in root canal treatment is Enterococcus faecalis. Several sterilization processes have been put forward for endodontic instruments; however, none were specific for off-centered NiTi rotary files that were contaminated by Enterococcus faecalis. Specific strategies and steps are necessary to achieve sterile off-centered NiTi rotary files that are free from Entercoccus faecalis. Aim: Compare the amount of Enterococcus faecalis on off-centered NiTi rotary files with variable taper and regressive taper after being processed using two different sterilization methods. Method: Eight test groups were established consisting of four off-centered NiTi rotary files with variable taper and four off-centered NiTi rotary files with regressive taper. Each of these four groups were divided into the following groups: negative control group, positive control group, Parashos sterilization group and lastly Linsuwanont sterilization group. Besides the negative control group, instruments from the other groups were used to perform root canal preparation of premolars that were contaminated by Enterococcus faecalis. The eight test groups were then tested for bacterial culture using chromogenic agar media to observe the presence and count the colony forming units of Enterococcus faecalis. Results: In the negative control group, no Enterococcus faecalis bacteria were found. The positive control group showed a large amount of Enterococcus faecalis present. The Parashos and Linsuwanont groups showed a variety in the amount of Enterococcus faecalis present, with the Parashos group showing a higher number of Enterococcus faecalis bacteria than the Linsuwanont group. Conclusion: The Linsuwanont sterilization method is the most effective method to sterilize Enterococcus faecalis bacteria from off-centered NiTi rotary files with regressive taper. The Parashos sterilization method was ineffective in sterilizing Enterococcus faecalis bacteria from both off-centered NiTi rotary files with variable taper and regressive taper.

Abstrak :
Keberadaan bakteri Enterococci dan Vancomycin Resistance Enterococci (VRE) di air sungai dapat menyebabkan infeksi apabila terpapar pada manusia. Penelitian ini memiliki tujuan untuk menganalisis konsentrasi bakteri Total Enterococci di Sungai Ciliwung dan menganalisis kosentrasi Vancomycin Resistance Enterococci (VRE) dan rasionya terhadap Total Enterococci (TE) dari hasil uji konfimasi dengan Antimicrobial Susceptibility Test (AST) menggunakan metode disk diffusion di Sungai Ciliwung. Hasil penelitian menunjukkan rata – rata TE sebesar 129,67 CFU/100 mL dan 153,33 CFU/100 mL untuk intake IPA Kedung Halang dan intake IPA Legong. Hasil konsentrasi VRE terkonfirmasi dengan menggunakan metode AST menunjukkan presumptive VRE yang hasilnya tidak semua resistant. Konsentrasi VRE terkonfirmasi diperoleh dari hasil konsentrasi rata – rata VRE di intake IPA Kedung Halang sebesar 1,111 × 103 CFU/100 mL dan intake IPA Legong sebesar 0 CFU/100 mL. Rasio VRE yang menunjukkan intake IPA Kedung Halang (27,10%) lebih besar dibandingkan dengan intake IPA Legong (0%). ...... The presence of Enterococci bacteria that live in the gastrointestinal (GI) tract. Enterococci bacteria can cause various infections, one example is urinary tract infection (UTI). This study has the aim of analyzing the concentration of Total Enterococci bacteria in the Ciliwung River and analyzing the concentration of Vancomycin Resistance Enterococci (VRE) and its ratio to Total Enterococci from the results of the confrimation test with the Antimicrobial Susceptibility Test (AST) using the disk diffusion method in the Ciliwung River. The method used in this study is for the concentration of total Enterococci using the membrane filtration method and the test results confirmed VRE with the Antimicrobial Susceptibility Test (AST) using the disk diffusion method. The results of the research that has been done show the average TE is Kedung Halang IPA intake 129.67 CFU/100 mL and Legong IPA intake 153.33 CFU/100 mL. The results of confirmed presumptive VRE using the AST method show presumptive VRE whose results are not all resistant. The confirmed VRE ratio was obtained from the results of the average VRE concentration at Kedung Halang IPA intake of 1.111 × 103 CFU/100 mL and Legong IPA intake of 0 CFU/100 mL. VRE ratio that shows Kedung Halang IPA intake (27.10%) greater than the Legong IPA intake (0%).