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"Kadar dopamin yang tidak seimbang dalam tubuh mengindikasikan berbagai macam kelainan neurologis seperti Parkinson disease (PD), skizofrenia, alzheimer, dan depresi. Salah satu metode pendeteksian dopamin adalah dengan sensor elektrokimia. Sensor elektrokimia merupakan metode pendeteksian yang murah dan dapat digunakan secara on-site. Contoh sensor elektrokimia adalah Screen-printed Carbon Electrode (SPCE). Modifikasi Screen-printed Carbon Electrode (SPCE) dengan nanopartikel merupakan pengembangan yang menarik dan terbukti meningkatkan selektivitas dan sensitivitas.Nanopartikel emas memilikikonduktivitas yang baik, area permukaan yang besar, dan biokompatibilitas yang tinggi. Penelitian ini dilakukan dengan memodifikasi SPCE dengan nanopartikel emas secara elektrokimia. Hasil yang didapatkan adalah pendeteksian dopamin secara optimum terjadi pada SPCE yang dideposisi (AuNPs-SPCE) selama 200 detik dan pada pH 6,5. Kemudian AuNPs-SPCE diujikarakterisasi melalui SEM dan UV-DRS. Uji analisis pada deteksi dopamin pada AuNPs-SPCE meliputi Limit of Detection (LOD), Limit of Quantification (LOQ), uji keberulangan, dan uji interferensi. Hasil uji linearitas adalah persamaan y = 0,420x + 0,2260 dengan R2= 0,9829 untuk SPCE dan sedangkan AuNPs-SPCE memiliki persamaan garis y = 2,817x + 1,456 dengan R2 =0,991 dengan slope yang mengindikasikan sensitivitas sensor. Hasil LOD dan LOQ untuk SPCE adalah 3,13 ?M dan 10,45 ?M. Sedangkan untuk AuNPs -SPCE LOD & LOQ-nya adalah 2,26 ?M dan 7,561 ?M.

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Unbalanced dopamine levels in the body indicate various kinds of neurological disorders such as Parkinson's disease (PD), schizophrenia, Alzheimer's, and depression. One method of detecting dopamine is by electrochemical sensors. Electrochemical sensors are inexpensive detection methods and can be used on-site. An example of an electrochemical sensor is the Screen-printed Carbon Electrode (SPCE). Modification of Screen-printed Carbon Electrode (SPCE) with nanoparticles is an interesting development and is proven to increase selectivity and sensitivity. Gold nanoparticles have good conductivity, large surface area, and high biocompatibility. This research was carried out by electrochemically modifying SPCE with gold nanoparticles. The results obtained were that the optimal detection of dopamine occurred in deposited SPCE (AuNPs-SPCE) for 200 seconds and at a pH of 6.5. Then the characterization of AuNPs-SPCE was tested by SEM and UV-DRS. Dopamine detection analysis tests on AuNPs-SPCE include Limit of Detection (LOD), Limit of Quantification (LOQ), repeatability test, and interference test. The results of the linearity test are the equation y = 0.420x + 0.2260 with R2 = 0.9829 for SPCE and while AuNPs-SPCE has the equation of the line y = 2.817x + 1.456 with R2 = 0.991 with the slope indicating the sensitivity of the sensor. The LOD and LOQ results for SPCE were 3.13 ?M and 10.45 ?M, respectively. Whereas for AuNPs -SPCE the LOD & LOQ were 2.26 ?M and 7,561 ?M."

"As a prolactin antagonist, dopamine binds to its receptor D2 and suppresses the secretion of prolactinfrom the pituitary gland. Required ligand that can interact with receptor so that dopamine can nolonger bound to its receptor. Ligands empirically derived from plants and have been proven toincrease human lactation. In silico study using molecular docking technique to identify ligandinteraction with dopamine D2 receptor (PDB: 1I15). The best posing and binding energy (ΔG) wereanalyzed and ranked to get potential dopamine D2 receptor inhibitor. Binding energy of apigenin,luteolin, kaempferol, quercetin, and myricetin with PDB: 1I15 gives value respectively -5.90, -5.67, -5.64, -5.28, -4.36 kcal/mol. Interaction occurred between the ligand and amino acid residue ASN 192,PRO 204, SER 209 formed hydrogen bonding and hydrophobic interaction with HIS 189."