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Abstrak :
Latar belakang: Suatu biomarker imunologis diperlukan untuk kasus yang berbatasan antara periodontitis kronis dan periodontitis agresif. Matrix Metalloproteinase-8 (MMP-8) merupakan suatu biomarker penentu risiko dan derajat keparahan penyakit periodonal serta evaluasi hasil perawatan periodontal. Tujuan: Menganalisis kadar MMP-8 pada cairan krevikular gingiva penderita periodontitis kronis dan periodontitis agresif. Metode: Penelitian ini mencakup 12 pasien periodontitis agresif, 17 pasien periodontitis kronis dan 6 subjek sehat. Kadar MMP-8 diukur dengan teknik ELISA. Hasil: Kadar MMP-8 pada periodonitits kronis tidak berbeda bermakna dengan periodontitis agresif (p>0,05). Kesimpulan: MMP-8 tidak dapat digunakan sebagai acuan diagnosis. ......Background: An immunologic biomarker is needed to distinguish between borderline cases between chronic periodontitis and agressive periodontitis. Matrix Metalloproteinase-8 (MMP-8) can be a risk profile of periodontal disease, disease progression and evaluation of periodontal treatment. Aim: Analyze MMP-8 levels in gingival crevicular fluid of chronic and aggressive periodontitis patients. Method: Sampel was collected from 6 healthy subjects, 12 subjects aggressive periodontitis and 17 chronic periodontitis subjects. MMP-8 levels were measured with ELISA. Result:MMP-8 levels in chronic periodontitis did not show any difference to the MMP-8levels in aggressive periodonitits (p>0,05). Conclusion: MMP-8 can not serve as a diagnostic parameter.

Abstrak :
Tujuan: Menganalisis perbandingan informasi self-perceived dengan diagnosis klinis status karies gigi anak usia 12-15 tahun. Metode: Penelitian dengan desain cross sectional menggunakan kuesioner untuk mendapatkan informasi self-perceived dan pemeriksaan indeks DMFT dan PUFA dilakukan pada 494 anak di enam SMP Negeri di Jakarta. Hasil: Hasil sensitivitas paling tinggi untuk indeks DMFT gold standard adalah dari pertanyaan kebutuhan perawatan, yaitu 0.86 dengan spesifisitas 0.23. Hasil spesifisitas paling tinggi adalah dari pertanyaan mengenai sakit gigi 0.89 dengan sensitivitas 0.26. Untuk indeks PUFA gold standard , sensitivitas tertinggi adalah pertanyaan pendapat keadaan gigi 0.92 dengan spesifisitas 0.57. Spesifisitas paling tinggi adalah pertanyaan mengenai sakit gigi 0.82 dengan sensitivitas 0.40. Prevalensi dan skor rata-rata DMFT dan PUFA berurutan adalah 68.4 dan 2.4; 17.6 dan 0.2. Kesimpulan: Kuesioner lebih sensitif terhadap indeks PUFA sebagai gold standard dibandingkan dengan indeks DMFT. Informasi self-perceived yang didapat dari kuesioner tidak dapat dengan baik mengevaluasi status klinis karies gigi karena kurangnya persepsi anak terhadap sehat dan sakit. ......Objective: To analyze the comparison of self perceived information and clinically diagnosed dental health status among children aged 12 15 years. Methods: A cross sectional study using questionnaire to obtain self perceived information and clinical examination using DMFT and PUFA indices was performed on 494 children in six junior high school in Jakarta. Results: The highest sensitivity for DMFT index as the gold standard was found in question about treatment need, 0.86 with specificity of 0.23. The highest specificity was found in question about dental pain 0.89 with specificity of 0.26. When using the PUFA index as the gold standard, the highest sensitivity was found in question about opinion regarding dental health, 0.92 with specificity of 0.57. The highest specificity was found in question about dental pain 0.82 with sensitivity of 0.40. The prevalence and mean DMFT and PUFA index was 68.4 and 2.4 17.6 and 0.2, respectively. Conclusion: Questionnaire was more sensitive using the PUFA index as the gold standard than the DMFT index. Self perceived information obtained from the questionnaire were of low value in evaluating dental health status due to the lack of children rsquo s health and disease perception.

Abstrak :
Latar Belakang: Kesehatan gigi dan mulut masih menjadi suatu permasalahan utama di Indonesia. Menurut Riskesdas tahun 2018 sebanyak 57,6% orang Indonesia memiliki permasalahan gigi dan mulut.Dalam risetnya, Prevalensi karies di Indonesia mencapai 88,8%. Faktor utama yang dapat menyebabkan permasalahan ini ialah bakteri patogen dalam rongga mulut, salah satunya yang paling patogenik ialah Streptococcus mutans. Karies terjadi ketika terjadi disbiosis dalam rongga mulut, yaitu ketika jumlah Streptococcus mutans berlebih sehingga menyebabkan kondisi asam pada rongga mulut. Tidak hanya menguntungkan bagi Streptococcus mutans, bakteri non-patogenik seperti Staphylococcus aureus akhirnya dapat memperburuk kondisi karies. Secara kimiawi, obat kumur Klorheksidin telah dimanfaatkan sebagai antibakteri yang secara akut dapat mengurangi jumlah bakteri rongga mulut. Tetapi dalam pemakaiannya ternyata klorheksidin menyebabkan efek samping jika dipakai untuk jangka panjang. Maka saat ini diperlukan pengembangan dari agen menggunakan herbal atau bahan alam. Salah satu bahan alam yang dapat digunakan sebagai agen antibakteri adalah Paku Acel. Kandungan yang terdapat daun paku acel yaitu terdapat flavonoid, terpenoid, tanin, saponin, dan alkanoid yang dapat berperan sebagai antibakteri. Tujuan: Mengetahui dan menganalisis efektivitas ekstrak daun paku acel (Nephrolepis cordifolia) dalam menghambat pertumbuhan dan membunuh koloni bakteri Streptococcus mutans dan Staphylococcus aureus serta membandingkan efektivitas ekstrak daun paku acel dengan chlorhexidine (kontrol positif). Metode: Efektivitas ekstrak daun paku acel terdapat bakteri Streptococcus mutans dan Staphylococcus aureus dilihat dari uji Kadar Hambat Minimum (KHM) mikrodilusi dengan ELISA Reader dan uji Kadar Bunuh Minimum (KBM) dengan konsentrasi ekstrak daun paku acel yang digunakan adalah 50%, 25%,12,5%, 6,25%, 3,25% Selanjutnya hasil tersebut dianalisis dengan uji statistik One Way Anova. Hasil: Ekstrak daun paku acel (Neprolephis cordifolia) dapat menghambat pertumbuhan bakteri Streptococcus mutans dan Staphylococcus aureus dengan nilai KHM 12,5% dan 6,25% secara berurutan. Melalui uji statistik One Way Anova didapatkan hasil bahwa terdapat perbedaan bermakna pada efektivitas ekstrak daun paku acel dengan chlorhexidine (p < 0,05). Kesimpulan: Ekstrak daun paku acel (Nephrolepis cordifolia) dapat menghambat pertumbuhan bakteri Streptococcus mutans dan Staphylococcus aureus sehingga dapat menjadi agen antibakteri yang efektif terhadap karies gigi tetapi kemampuannya masih dibawah klorheksidin ......Background: Dental and oral health is still a major problem in Indonesia. According to Riskesdas in 2018 as many as 57.6% of Indonesians had dental and mouth problems In his research, the prevalence of caries in Indonesia reached 88.8%. The main bacterial factors that can cause this problem are pathogens in the oral cavity, one of the most pathogenic is Streptococcus mutans. Caries occur when dysbiosis occurs in the oral cavity, namely when the amount of Streptococcus mutans is excessive, causing an acidic condition in the oral cavity. Not only beneficial for Streptococcus mutans, but non-pathogenic bacteria such as Staphylococcus aureus can also finally oppose caries conditions. Chemically, Clorhexidine mouthwash has been used as an antibacterial which can acutely reduce the number of bacteria in the oral cavity. But in its use it turns out that Clorhexidine causes side effects if used for the long term. So at this time it is necessary to develop agents using herbs or natural ingredients. One of the natural ingredients that can be used as an antibacterial agent is Erect Sword Fern. Erect Sword Fern or Nephrolepis cordifolia has many benefits in the medical field, one of which is as an antibacterial agent. Objectives: To determine and analyze the effectiveness of acel nail extract (Nephrolepis cordifolia) in inhibiting the growth and killing of Streptococcus mutans and Staphylococcus aureus bacteria colonies and to compare the effectiveness of acel nail extract with chlorhexidine (positive control). Method: The effectiveness of acel fern leaf extract contained Streptococcus mutans and Staphylococcus aureus as seen from the microdilution Minimum Inhibitory Concentration (MIC) test with ELISA Reader and Minimum Bactericidal Concentration (MBC) test with the concentration of acel fern leaf extract used was 50%, 25%, 12.5%, 6 .25%, 3.25% Then the results were analyzed with the One Way Anova statistical test. Results: Leaf extract of Erect Sword Fern (Neprholepis cordifolia) only can inhibit growth Streptococcus mutans and Staphylococcus aureus bacteria with MIC values ​​of 12,5% and 6,25%. Through the One Way Anova statistical test, it was found that there was a significant difference in the effectiveness of Erect Sword Fern leaf extract and Clorhexidine (p <0.05). Conclusion: Erect Swordfern leaf extract (Nephrolepis cordifolia) can inhibit bacterial growth Streptococcus mutans and Staphylococcus aureus bacteria so that it can be an effective antibacterial agent against dental caries but its ability is still below Clorhexidine

Abstrak :
Latar belakang: Karies gigi merupakan penyakit gigi multifaktorial yang dipengaruhi oleh banyak faktor. Salah satunya adalah peran genetik. Banyak penelitian mengenai hubungan antara peran genetik kerentanan karies gigi telah dilakukan, terutama polimorfisme gen pada gen gigi tertentu. Tujuan: Untuk memperkirakan kemungkinan asosiasi gen amelogenin (AMELX) dan non-amelogenin (ENAM dan AMBN) terhadap kerentanan karies gigi berdasarkan populasi. Metode: Enam database elektronik, terdiri dari: PubMed, Scopus, EBSCO, Cochrane Library, ScienceDirect, dan Wiley Online Library dilakukan secara rumit sebagai pencarian data lanjutan untuk tanggal publikasi hingga Februari 2019. Proses menyeluruh dalam studi ulasan ini dilakukan dengan menggunakan Preferred Item pelaporan untuk pedoman Tinjauan Sistematik dan Meta-analisis (PRISMA). Bagian meta-analisis diimplementasikan dengan menggunakan dua perangkat lunak: Review Manager 5.4 dan Comprehensive Meta-analysis 2.0. Hasil: Empat belas studi termasuk dalam analisis kualitatif (tinjauan sistematis) dan kuantitatif (meta-analisis). Hasil analisis kuantitatif menyimpulkan bahwa ENAM rs3796704 berhubungan sebagai faktor protektif terhadap kerentanan karies gigi, terutama pada alel A (OR=0,7; p-value=0,009), genotipe AG (OR=0,7; p-value=0,03), dan genotipe AA (dominan) (OR=0.7; p-value=0.02). ENAM rs3796704 juga memiliki faktor protektif yang signifikan terhadap karies gigi pada etnis Kaukasia dan kelompok usia anak-anak. Namun, tidak ada hubungan bermakna antara AMELX rs946252, AMELX rs17878486, AMELX rs6639060, AMELX rs2106416, ENAM rs1264848, ENAM rs3796703, AMBN rs4694075, dan AMBN rs34538475 terhadap kerentanan karies gigi. Kesimpulan: ENAM rs3796704 memiliki hubungan yang signifikan terhadap kerentanan gigi karies. ......Background: Dental caries is a multifactorial dental disease that is influenced by many factors. One of them is genetic role. Many studies regarding the association between genetic roles dental caries susceptibility have been conducted, especially gene polymorphisms in certain dental genes. Aim: estimate the possible association of amelogenin (AMELX) and non-amelogenin (ENAM and AMBN) genes to dental caries susceptibility in population-based. Methods: Six electronic databases, consist of: PubMed, Scopus, EBSCO, Cochrane Library, ScienceDirect, and Wiley Online Library are carried out intricately as data advanced searches for publication date until February 2019. The thorough process in this review study is carried by using the Preferred Reporting items for Systematic Reviews and Meta-analyses (PRISMA) guideline. The meta-analysis part is implemented by using two softwares: Review Manager 5.4 and Comprehensive Meta-analysis 2.0. Results: Fourteen studies are included in both qualitative (systematic review) and quantitative (meta-analysis) analyses. The result of the quantitative analysis concluded that ENAM rs3796704 is associated as protective factor to dental caries susceptibility, especially in allele A (OR=0.7; p-value=0.009), genotype AG (OR=0.7; p-value=0.03), and genotype AA (dominant) (OR=0.7; p-value=0.02). ENAM rs3796704 also has a significant protective factor to dental caries in caucasian ethnicity and children age group. However, there was no significant association of AMELX rs946252, AMELX rs17878486, AMELX rs6639060, AMELX rs2106416, ENAM rs1264848, ENAM rs3796703, AMBN rs4694075, and AMBN rs34538475 to dental caries susceptibility. Conclusion: ENAM rs3796704 plays a significant association to dental caries susceptibility.

Abstrak :
ABSTRAK
Lesi white spot adalah tanda awal demineralisasi email yang dapat berkembang menjadi karies. Derajat mineralisasi email dapat ditentukan dengan melakukan uji kekerasan mikro email. Penambahan agen antibakteri seperti nanopartikel TiO2 ke dalam bahan adesif resin ortodonti nanokomposit TiO2 diharapkan dapat mencegah demineralisasi email. Tujuan penelitian ini adalah untuk mengetahui perbedaan kekerasan mikro email pada gigi yang dipasangi braket menggunakan nanokomposit TiO2 dengan gigi sehat.Braket dipasang pada permukaan gigi premolar menggunakan Transbond XT kelompok 1 , nanokomposit TiO2 1 kelompok 2 , nanokomposit TiO2 2 kelompok 3 . Kelompok 4 adalah kelompok kontrol yang tidak diberi perlakuan apapun sebelum dilakukan uji kekerasan mikro email. Sampel dari kelompok 1, 2, dan 3 direndam dalam kaldu BHI yang mengandung Streptococcus mutans dan disimpan dalam inkubator 37oC selama 30 hari. Derajat mineralisasi email didapatkan dengan Vickers microhardness test pada potongan melintang gigi yang berjarak 200 m ke arah servikal dasar braket.Nilai kekerasan mikro email berbeda bermakna antara tiap kelompok penelitian. Nilai tertinggi ditemukan pada kelompok kontrol, diikuti dengan nanokomposit TiO2 2 , nanokomposit TiO2 1 , dan Transbond XT.Kesimpulan: Penelitian ini menemukan bahwa gigi yang dipasangi braket menggunakan nanokomposit TiO2 memiliki nilai kekerasan mikro email yang lebih tinggi dibandingkan gigi yang dipasangi menggunakanTransbond XT.

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ABSTRACT
White spot is an early sign of enamel demineralization which may lead to development of dental caries. Enamel mineralization status could be determined by examining microhardness number of the enamel. Addition of antibacterial agent such as TiO2 nanoparticles into orthodontic adhesive TiO2 nanocomposite is expected to prevent enamel demineralization. Objective of this study is to evaluate the difference between enamel microhardness number of teeth bonded with TiO2 nanocomposite and healthy teeth.Bracket was bonded to the premolar using Transbond XT group 1 , 1 TiO2 nanocomposite group 2 , 2 TiO2 nanocomposite group 3 . Group 4 was the control group and not given any treatment prior to the microhardness test. The samples of group 1, 2, and 3 were soaked in BHI solution containing Streptococcus mutans then stored in 37oC incubator for 30 days. Enamel mineralization status were determined on cross sectioned tooth 200 m cervical to the bracket by Vickers microhardness test.The micro hardness value were significantly different between every group, with highest value were obtained by control group, followed by 2 TiO2 nanocomposite, 1 TiO2 nanocomposite, then Transbond XT.Conclusion This study found that teeth bonded with TiO2 nanocomposite have higher enamel microhardness number than teeth bonded with Transbond XT.

Abstrak :
Tujuan: Penelitian ini bertujuan untuk menguji reliabilitas kuesioner frekuensi konsumsi makanan kariogenik, mengetahui status karies gigi, dan hubungan frekuensi konsumsi makanan kariogenik dengan status karies gigi pada anak usia 15-16 tahun di DKI Jakarta. Metode: Survei epidemiologi dilakukan dengan desain penelitian cross sectional. Pemeriksaan karies gigi dilakukan oleh satu pemeriksa, menggunakan indeks DMFT. Indeks DMFT digunakan untuk mencatat prevalensi karies gigi berdasarkan kriteria WHO. Selain itu juga memberikan FFQ/ Food Frequency Quetionare yang dijawab oleh subjek untuk mendapatkan informasi mengenai frekuensi konsumsi makanan kariogenik, karakteristik anak, dan keadaan sosiodemografi. Data kemudian dianalisis dengan analitik komparatif. Hasil: Total sampel sebanyak 471 anak, dengan prevalensi yang mengalami karies gigi sebesar 75,4% dengan rata-rata 2,72. Kesimpulan: Status karies gigi pada anak usia 15-16 tahun di DKI Jakarta tergolong moderate, dengan rata-rata 2,72. Kedua kuesioner yang digunakan dalam penelitian ini adalah reliabel. Karies gigi berhubungan signifikan dengan jenis kelamin, pendidikan ibu dan item makanan yogurt, perment mint, kripik, dan minuman bersoda. ......Objective: This study aimed to examine the reliability of the questionnaire frequency of cariogenic food consumption, determine the dental caries status, and the relationship between the frequency of cariogenic food consumption and dental caries status in children aged 15-16 years in DKI Jakarta. Method: Epidemiology surveys were conducted with cross sectional study design. Dental caries was examined by one examiner, using DMFT index. DMFT index is used to record the prevalence of dental caries based on WHO criteria. FFQ / Food Frequency Quetionare answered by the subject to get information about the frequency of consumption of cariogenic foods, children's characteristics, and sociodemographic conditions. Data were analyzed with comparative analytic. Results: A total sample of 471 children, with a prevalence of dental caries of 75.4% with an average of 2.72. Conclusion: Dental caries status in children aged 15-16 years in DKI Jakarta is classified as moderate, with an average of 2.72. Both of questionnaires used in this study are reliable. Dental caries is significantly associated with gender, maternal education and food items such as yogurt, mint, mint, and carbonated drinks.

Abstrak :
Prevalensi karies gigi yang ditunjukkan dengan decayed, missing dan filled teeth (DMF-T) masih merupakan masalah kesehatan masyarakat, termasuk di Indonesia. Penelitian ini bertujuan untuk mengetahui peran faktor individu, rumahtangga dan kabupaten/kota terhadap kejadian karies gigi guna menyusun model pengendalian karies gigi di Kepulauan Bangka Belitung (provinsi dengan riwayat karies gigi tertinggi). Desain penelitian ini campuran (hybrid) antara ecological study dan cross-sectional. Pengumpulan data dilakukan dengan wawancara menggunakan kuesioner, pemeriksaan kesehatan gigi, pengambilan sampel air dan wawancara mendalam. Analisis data menggunakan regresi logistik multilevel (dengan mixed-effect model). Hasil penelitian menunjukkan faktorfaktor pada tingkat individu (frekuensi menggosok gigi, kebersihan gigi dan mulut dan kebiasaan makan makanan asam/bercuka), tingkat rumahtangga (jenis sumber dan keasaman air) dan tingkat kabupaten/kota (ketersediaan perawat gigi dan dokter gigi, angka gizi buruk dan besar anggaran kesehatan per kapita) berpengaruh terhadap prevalensi karies gigi berat pada penduduk dewasa, dimana secara keseluruhan dapat menjelaskan variasi risiko karies gigi sebesar 73,6%. Model pengendalian karies gigi yang sesuai dengan kondisi Kepulauan Bangka Belitung adalah menggabungkan pengendalian faktor pada tingkat individu, rumahtangga dan kabupaten/kota. ......Dental caries prevalence, indicated by decayed, missing and filled teeth (DMF-T), remains a global public health problem, including Indonesia. The objective of this research was to address the role of individual factors, households, and regency/municipality in explaining dental caries incidence, in order to formulate a model to control dental caries in Kepulauan Bangka Belitung—the province with the highest dental caries prevalence in Indonesia. This research was designed as a combination (hybrid) of cross-sectional and ecological studies. Quantitative and qualitative data were collected through interview, dental health examination by dentists, water sampling, and in-depth interviews. A multilevel logistic regression (mixed-effect) model was fitted to the data. The results show that the explanatory variables at individual (frequency of teeth brushing, dental and mouth hygiene, and acidic food consumption), household (main water source and acidity), and regency/municipality (availability of dentist and dental nurse, malnutrition, and per capita health budget) levels influenced the prevalence of severe dental caries among adults; they all explained 73.6% of the variation in risk of dental caries. The appropriate disease control model, given the local conditions of Kepulauan Bangka Belitung Province, is one that integrates control of risk factors at individual, household, and regency/municipality levels.

Abstrak :
Background: Protein is one of the nutrients that teeth need during growth, maturation and, after eruption. Protein consists of plantprotein and animal protein. Plantprotein has the highest average percentage of daily protein consumption inIndonesia.Plantprotein is found in nuts, tofu, and tempeh. The content of food sources of plantprotein has many benefits that are good for oral health.At the age of 8-9 years permanent first molar teeth have erupted they can experience caries within 1-2 years after eruption because of their morphological and functional characteristics. In children, dental caries is one of the most frequent chronic infectious diseases occur and have close links with nutrition.While snacks may also contribute to oral health problems.Aim: To determine the relationship between the level of consumption of plant protein and cariogenic food with the occurrence of permanent dental first molar teeth in children aged 8-9 years in Central Jakarta. Methods: This study isa cross-sectional observational analytic study. The subjects of the study were 109 children aged 8-9 years who were studying State Primary School in Central Jakarta. Teeth examined for permanent first molar teeth that have been erupted. The measuring instrument used was a questionnaire and caries assessment using classification (ICDAS). Result:.The results of the study showed the frequency distribution of caries as follows; 1.8% caries free, 63.3% enamel caries, and 34.9% dentin-pulp caries. Meanwhile, consumption of vegetable protein with caries depth and consumption of snack foods with depth of caries both showed very weak correlation results (r = 0.00-0.199) and there was no statistically significant difference (P> 0.05).Conclusion: There is a very weak correlation between consumption of vegetable protein and snack food with the depth of caries in permanent first molars in children aged 8-9 years in Central Jakarta, and there is no statistically significant difference

Abstrak :
Tujuan: Mengetahui perbedaan persepsi antara ortodontis dengan masyarakat awam serta pria dengan wanita dalam menilai hasil perawatan hipodonsia insisif lateral rahang atas bilateral. Metode penelitian: Bentuk, warna, dan tepi gingiva gigi insisif lateral rahang atas bilateral dimodifikasi secara digital sebagai simulasi hasil perawatan hipodonsia insisif lateral rahang atas bilateral. Ketiga foto hasil modifikasi dilampirkan pada kuesioner berbasis digital lalu dinilai oleh 100 ortodontis dan 100 masyarakat awam, terdiri dari 100 pria dan 100 wanita. Penilaian dilakukan dengan metode Visual Analogue Scale (VAS) dan uji statistik dilakukan dengan uji-t tidak berpasangan. Hasil: Perbedaan bermakna secara statistik ditemukan pada persepsi ortodontis dan masyarakat awam terhadap foto perawatan dengan substitusi kaninus tanpa rekonturing dan foto perawatan dengan protesa. Kesimpulan: Ortodontis dan masyarakat awam memiliki persepsi yang berbeda terhadap hasil perawatan hipodonsia insisif lateral rahang atas bilateral, sedangkan jenis kelamin tidak mempengaruhi persepsi terhadap hasil perawatan hipodonsia insisif lateral rahang atas bilateral. ......Objectives: This study aimed to compare the perception of Indonesian orthodontists and laypeople as well as men and women to the treatment of bilateral upper lateral incisor hypodontia. Methods: Shape, color, and gingival margin of bilateral upper lateral incisor were digitally modified as a simulation of bilateral upper lateral incisor hypodontia treatment result. Three modified photos were presented in a digital-based questionnaire and assessed by 100 orthodontists and 100 laypeople, consisting of 100 men and 100 women. The assessment was done using Visual Analogue Scale (VAS) and unpaired t-test was used to process the data. Results: Statistically significant differences were found between the perception of orthodontists and laypeople to the treatment utilizing canine substitution without recontouring, along with the treatment using prosthesis. Conclusions: Orthodontists and laypeople have different perceptions to the treatment results of bilateral upper lateral incisor hypodontia, while gender does not affect the perception to the treatment results.