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Abstrak :
[Kanker kolorektal merupakan keganasan ketiga terbanyak di Indonesia dengan 95% diantaranya adalah adenokarsinoma kolon. Saat ini, tatalaksana yang dapat diberikan berupa bedah reseksi atau laparoskopi masih terbatas khususnya pada kanker kolon stadiur akhir. Sehingga, masih diperlukan penelitian untuk menemukan terapi alternatif untuk mendukung tatalaksana yang ada. Salah satunya adalah kulit buah manggis yang dikatakan memiliki berbagai manfaat, termasuk antikanker. Ekstrak etanol kulit buah manggis (Garcinia mangostana l.) pada penelitian ini diuji efek sitotoksisitasnya terhadap sel adenokarsinoma kolon (C2BBE1) secara in vitro. Kulit manggis utuh segar dikeringkan, ditumbuk menjadi serbuk, dimaserasi dalam alkohol 99%, kemudian dievaporasi untuk menghasilkan crude extract kulit manggis. Dilakukan uji KLT dan fitokimia untuk mengidentifikasi kandungan ekstrak. Digunakan delapan variasi konsentrasi ekstrak, yaitu 6,2 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, dan 800 μg/ml yang dilarutkan dalam DMSO dan media RPMI sebelum ditambahkan ke sel uji. Sel uji merupakan sel adenokarsinoma kolon dari Departemen Patologi Anatomik FKUI/RSCM yang sebelum digunakan sudah ditumbuhkan, diamati pertumbuhannya, dihitung kepadatannya, dan dipelihara dalam medium kultur komplit, pada suhu 37 °C dengan kandungan 5% CO2 pada inkubator. Penambahan ekstrak dilakukan saat pertumbuhan sel konfluens dan diinkubasi kembali selama 48 jam untuk kemudian diamati di bawah mikroskop dino-eye dan dilakukan uji sitotoksisitas dengan metode MTT assay. Didapatkan nilai %inhibisi proliferasi sel dengan pemberian ekstrak berbeda bermakna terhadap kontrol dengan nilai p=0.015 (< 0,05) dengan uji Kruskal Wallis. Nilai IC50nya adalah 1,11 μg/ml yang berarti ekstrak yang mengandung polifenolat (termasuk xanton) tersebut bersifat sitotoksik kuat terhadap sel uji., Colorectal cancer is the third most found cancer in Indonesia in which 95% of them are colon adenocarcinoma. Today, the therapy is still limited in resection surgey or laparoscopy which is not efficient especially in late stadium. Therefore, alternative treatments are needed to support existing therapies. One of them is Mangosteen Pericarp which is known for its many benefits including as an anticancer. In this study, mangosteen (Garcinia mangostana Linn) pericarp ethanol extract’s cytotoxicity is tested on colon adenocarcinoma cells (type C2BBE1). The fruit’s pericarp is peeled, dried, ground into powder, macerated in 99% ethanol, then evaporated to create a crude extract of mangosteen pericarp. TLC and phytocemical screening is done to detect the components of the extract. There were 8 variations of extract concentration; 6.2 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml which were dissolved in DMSO and RPMI media before given to tested cell lines. Tested cell lines were available from anatomic pathology laboratory of FKUI RSCM which were cultured, monitored, counted, and inccubated in culture media under moist ciurcumstances (370C, 5% CO2), The extracts are given to cell lines which were 50% confluent then incubated for 48 hours. The cells then observed under microscope with dino-eye camera and tested using MTT assay kit to know the cytotoxycity. The results show significant difference between inhibition percentage of tested extracts to control with the value of p=0.015 (< 0,05) measured with Kruskal Wallis test. The IC50 value is 1.11μg/ml which means that the xanthon containing extract is highly cytotoxic to the tested cell lines]

Abstrak :
[Buah manggis (Garcinia mangostana Linn) merupakan salah satu buah tropis dari Asia Tenggara seperti Indonesia dan kulitnya biasanya digunakan sebagai obat tradisional untuk mengatasi inflamasi dan mikroorganisme. Selain itu, kulit buah manggis juga diperkirakan dapat digunakan sebagai antikanker. Tujuan dari penelitian ini adalah mengetahui pengaruh ekstrak etanol kulit buah manggis terhadap viabilitas sel Raji secara in vitro melalui uji sitotoksisitas. Ekstrak etanol kulit buah manggis didapatkan melalui proses maserasi dan evaporasi dengan rotary evaporator. Ekstrak dibagi menjadi beberapa konsentrasi, yaitu 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, dan 800 μg/ml, kemudian diujikan ke sel Raji dan diinkubasi selama 48 jam. Uji sitotoksisitas yang digunakan adalah metode MTT-assay. Sifat sitotoksisitas ekstrak tersebut ditentukan oleh nilai IC50, lalu uji kemaknaan yang digunakan adalah Kruskal-Wallis. Hasil analisis menunjukkan nilai IC50 sebesar 3,07 μg/ml (p = 0,02). Kesimpulan dari penelitian ini adalah ekstrak etanol kulit buah manggis bersifat sitotoksik kuat terhadap viabilitas sel Raji dan ditemukan adanya perbedaan bermakna antar kelompok. Hasil uji Post Hoc memperlihatkan terdapat perbedaan bermakna antara kelompok kontrol dan kelompok perlakuan dengan konsentrasi 6,25 μg/ml dengan kelompok perlakuan lain.;Mangosteen (Garcinia mangostana Linn) is one of tropical fruit from south east Asia such as Indonesia and its pericarp usually used as traditional medicine for anti-inflammatory and anti-microorganism. Mangosteen pericarp is also expected can be used as anticancer. The aim of this study was to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on viability of Raji cells. The extract was obtained by maceration and evaporation process with rotary evaporator. The extract was divided into several concentration, such as 6.25 μg/ml, 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml, then it was tested with Raji cells and incubated during 48 hours. The cytotoxic effect against Raji cells is evaluated by MTT-assay. The cytotoxicity level of the extract is determined by IC50 value, then the significance test is used Kruskal-Wallis. The result of analysis showed that IC50 value was 3.07 μg/ml (p = 0.02). The conclusion of this research were the mangosteen pericarp ethanol extract has high cytotoxicity for viability Raji cells and there was a significant difference between groups. Post Hoc test result showed there were significant difference between control and 6.25 μg/ml group which compared with other groups;Mangosteen (Garcinia mangostana Linn) is one of tropical fruit from south east Asia such as Indonesia and its pericarp usually used as traditional medicine for anti-inflammatory and anti-microorganism. Mangosteen pericarp is also expected can be used as anticancer. The aim of this study was to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on viability of Raji cells. The extract was obtained by maceration and evaporation process with rotary evaporator. The extract was divided into several concentration, such as 6.25 μg/ml, 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml, then it was tested with Raji cells and incubated during 48 hours. The cytotoxic effect against Raji cells is evaluated by MTT-assay. The cytotoxicity level of the extract is determined by IC50 value, then the significance test is used Kruskal-Wallis. The result of analysis showed that IC50 value was 3.07 μg/ml (p = 0.02). The conclusion of this research were the mangosteen pericarp ethanol extract has high cytotoxicity for viability Raji cells and there was a significant difference between groups. Post Hoc test result showed there were significant difference between control and 6.25 μg/ml group which compared with other groups, Mangosteen (Garcinia mangostana Linn) is one of tropical fruit from south east Asia such as Indonesia and its pericarp usually used as traditional medicine for anti-inflammatory and anti-microorganism. Mangosteen pericarp is also expected can be used as anticancer. The aim of this study was to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on viability of Raji cells. The extract was obtained by maceration and evaporation process with rotary evaporator. The extract was divided into several concentration, such as 6.25 μg/ml, 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml, then it was tested with Raji cells and incubated during 48 hours. The cytotoxic effect against Raji cells is evaluated by MTT-assay. The cytotoxicity level of the extract is determined by IC50 value, then the significance test is used Kruskal-Wallis. The result of analysis showed that IC50 value was 3.07 μg/ml (p = 0.02). The conclusion of this research were the mangosteen pericarp ethanol extract has high cytotoxicity for viability Raji cells and there was a significant difference between groups. Post Hoc test result showed there were significant difference between control and 6.25 μg/ml group which compared with other groups]

Abstrak :
[Angka kejadian penyakit mieloma multipel kecil, yaitu 0,8% di dunia dan 0,6% di Asia Tenggara dari seluruh kasus kanker yang ada. Namun, penyakit ini terjadi secara asimtomatik sehingga sulit didiagnosis, belum dapat disembuhkan, dan mudah mempengaruhi organ dalam tubuh. Kulit buah manggis yang jarang dimanfaatkan diketahui mengandung senyawa xanton (polifenolat) yang memiliki aktivitas antikanker. Penelitian in vitro menggunakan sel jalur p3x63ag8 untuk menemukan ada tidaknya efek sitotoksisitas ekstrak etanol kulit buah manggis serta IC50. Sel dibagi menjadi 9 kelompok, yaitu 1 kelompok kontrol dan 8 kelompok perlakuan dengan konsentrasi 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, dan 800 μg/ml. Data diambil dengan metode MTT assay dan hasilnya berupa nilai optical density. Setelah inkubasi 48 jam menggunakan ekstrak etanol kulit buah manggis, hasil persamaan garis diketahui IC50 nya adalah 5,41 μg/ml. Analisis statistik dengan Kruskal Wallis menghasilkan adanya perbedaan efek sitotoksik pada konsentrasi yang berbeda . Uji Post Hoc didapatkan perbedaan bermakna antara kelompok kontrol dan kelompok perlakuan 6,25 μg/ml dengan kelompok perlakuan lain.;Multiple myeloma disease has small incidence, namely 0,8% in the world and 0,6% in Southeast Asia of all cancer cases. However, the diasease occurs in asymptomatic that so difficult to be diagnosed, can not be cured, and affects many organs. The mangosteen pericarp which rarely used evidently contain xanthone (polifenolat) compound which have anticancer activity. Research in in vitro manner using cell lines p3x63ag8 to discover the presence of cytotoxicity effect of mangosteen pericarp ethanol extract and the IC50. Cells was divided into 9 groups, 1 control group and 8 treatment groups (consentrations: 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml). Data taken by MTT assay method and the result is optical density value. After 48-hours incubation period and the result in line equation, found that IC50 was 5.41 ug / ml. Statistical analysis with Kruskal Wallis declared differences in the cytotoxic effects of different concentrations.Post Hoc test found significant difference beetwen the control group and the treatment group of 6.25 ug / ml just than other groups;Multiple myeloma disease has small incidence, namely 0,8% in the world and 0,6% in Southeast Asia of all cancer cases. However, the diasease occurs in asymptomatic that so difficult to be diagnosed, can not be cured, and affects many organs. The mangosteen pericarp which rarely used evidently contain xanthone (polifenolat) compound which have anticancer activity. Research in in vitro manner using cell lines p3x63ag8 to discover the presence of cytotoxicity effect of mangosteen pericarp ethanol extract and the IC50. Cells was divided into 9 groups, 1 control group and 8 treatment groups (consentrations: 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml). Data taken by MTT assay method and the result is optical density value. After 48-hours incubation period and the result in line equation, found that IC50 was 5.41 ug / ml. Statistical analysis with Kruskal Wallis declared differences in the cytotoxic effects of different concentrations.Post Hoc test found significant difference beetwen the control group and the treatment group of 6.25 ug / ml just than other groups, Multiple myeloma disease has small incidence, namely 0,8% in the world and 0,6% in Southeast Asia of all cancer cases. However, the diasease occurs in asymptomatic that so difficult to be diagnosed, can not be cured, and affects many organs. The mangosteen pericarp which rarely used evidently contain xanthone (polifenolat) compound which have anticancer activity. Research in in vitro manner using cell lines p3x63ag8 to discover the presence of cytotoxicity effect of mangosteen pericarp ethanol extract and the IC50. Cells was divided into 9 groups, 1 control group and 8 treatment groups (consentrations: 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml). Data taken by MTT assay method and the result is optical density value. After 48-hours incubation period and the result in line equation, found that IC50 was 5.41 ug / ml. Statistical analysis with Kruskal Wallis declared differences in the cytotoxic effects of different concentrations.Post Hoc test found significant difference beetwen the control group and the treatment group of 6.25 ug / ml just than other groups]

Abstrak :
[Kanker merupakan salah satu penyebab kematian utama di dunia, termasuk Indonesia. Berbagai penelitian dilakukan untuk mencari alternatif terapi kanker. Kulit manggis dipercaya mempunyai kandungan senyawa yang bersifat sitotoksik terhadap sel kanker. Penelitian ini bertujuan untuk mengetahui efek sitotoksisitas ekstrak etanol kulit manggis terhadap sel limfoma Hodgkin. Ekstrak yang digunakan berasal dari proses ekstraksi kulit manggis dengan pelarut etanol menggunakan Vaccum Rotary Evaporator pada tekanan 1 atm dengan suhu 60o C. Ekstrak kulit manggis diberikan dalam 8 konsentrasi berbeda yaitu 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, dan 800 μg/ml. Sitotoksisitas dinilai dengan uji MTT-assay untuk mendapat nilai IC50. Hasil penelitian menunjukkan bahwa ekstrak etanol kulit manggis mempunyai efek sitotoksik terhadap sel limfoma Hodgkin dengan nilai IC50 sebesar 5.6 μg/ml. Uji kemaknaan menggunakan uji Kruskal-Wallis menunjukkan nilai p = 0.008 (p ≤ 0.05). Kesimpulan dari penelitian ini yaitu ekstrak etanol kulit manggis mempunyai efek sitotoksik kuat terhadap sel Limfoma Hodgkin.;Cancer is one of the leading cause of death in the world, including Indonesia. Various studies have been done to seek alternative cancer therapy. Mangosteen pericarp is believed to have substance that are cytotoxic to cancer cells. The purpose of this study is to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on Hodgkin Lymphoma cells. The extract used in this study is obtained from the mangosteen pericarp extraction using Vacuum Rotary Evaporator at a pressure of 1 atm and temperature of 60o C. Mangosteen pericarp extract is given in eight different concentration of 6.25 ug / ml, 12.5 pg / ml, 25 mg / ml, 50 pg / ml, 100 pg / ml, 200 mg / mL, 400 mg / ml, and 800 ug / ml. Cytotoxicity was assessed using MTT-assay test to obtain IC50 values. The results showed that ethanol extract of mangosteen pericarp has a cytotoxic effect on Hodgkin lymphoma cells with IC50 value of 5.6 ug / ml. The data were analyzed using Kruskal-Wallis test and had a p value of 0.008 (p ≤ 0.05). The conclusion of this study is that ethanol extract of mangosteen pericarp has a strong cytotoxic effect on Hodgkin lymphoma cells, Cancer is one of the leading cause of death in the world, including Indonesia. Various studies have been done to seek alternative cancer therapy. Mangosteen pericarp is believed to have substance that are cytotoxic to cancer cells. The purpose of this study is to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on Hodgkin Lymphoma cells. The extract used in this study is obtained from the mangosteen pericarp extraction using Vacuum Rotary Evaporator at a pressure of 1 atm and temperature of 60o C. Mangosteen pericarp extract is given in eight different concentration of 6.25 ug / ml, 12.5 pg / ml, 25 mg / ml, 50 pg / ml, 100 pg / ml, 200 mg / mL, 400 mg / ml, and 800 ug / ml. Cytotoxicity was assessed using MTT-assay test to obtain IC50 values. The results showed that ethanol extract of mangosteen pericarp has a cytotoxic effect on Hodgkin lymphoma cells with IC50 value of 5.6 ug / ml. The data were analyzed using Kruskal-Wallis test and had a p value of 0.008 (p ≤ 0.05). The conclusion of this study is that ethanol extract of mangosteen pericarp has a strong cytotoxic effect on Hodgkin lymphoma cells]

Abstrak :
Indonesia merupakan negara biodiversitas tinggi yang memiliki 7.000 dari 30.000 jenis tumbuhan yang dapat digunakan sebagai obat tradisional. Salah satunya tanaman bajakah tampala (Spatholobus littoralis Hassk.) yang mengandung berbagai senyawa antioksidan sehingga diperkirakan dapat mengakibatkan kematian pada sel kanker. Beberapa studi menunjukkan bahwa ekstrak bajakah tampala dapat mengakibatkan apoptosis pada sel T47D kanker payudara selama 24 jam. Namun, penelitian terkait uji sitotoksisitas ekstrak bajakah tampala terhadap sel kanker payudara MCF-7 belum ditemukan sehingga penelitian ini perlu dilakukan. Tujuan dari penelitian ini yaitu untuk menemukan konsentrasi optimal ekstrak bajakah tampala yang dapat menghambat 50% proliferasi sel. serta mengetahui efek sitotoksisitas ekstrak bajakah tampala. Metode penelitian ini menggunakan sel MCF-7 dan batang bajakah tampala yang diekstraksi dengan metode maserasi menggunakan etanol 96%. Selanjutnya, dilakukan uji MTT dan flow cytometry. Hasil penelitian menunjukkan ekstrak etanol bajakah tampala memiliki nilai IC50 sebesar 104 ppm dan menghasilkan efek sitotoksisitas yang dapat menyebabkan apoptosis pada sel MCF-7. Maka dapat disimpulkan bahwa ekstrak bajakah tampala dengan pelarut etanol 96% tergolong dalam kelompok senyawa yang bersifat kurang aktif sebagai antikanker. ......Indonesia is a high biodiversity country with 7,000 of 30,000 plant species that can be used as traditional medicine. One of them is the bajakah tampala plant (Spatholobus littoralis Hassk.) which contains various antioxidant compounds that are assumed can cure cancer. Further studies revealed that bajakah tampala extract can induce apoptosis in T47D breast cancer cell lines. However, studies related to the cytotoxicity test of bajakah tampala extract on MCF-7 breast cancer cells have not been discovered. Hence, it is necessary to analyze the cytotoxicity effect of bajakah tampala extract on MCF-7 breast cancer cells. This study aimed to find the optimal concentration of bajakah tampala extracts that can inhibit 50% of cell proliferation and to know the cytotoxicity effect of bajakah tampala extract. This research used MCF-7 breast cancer cell lines and bajakah tampala stems. The stems were extracted by maceration method using 96% ethanol. These research methods are viability tests with MTT reagents and anti-cancer activity tests using flow cytometry. The results showed that the ethanol extract of bajakah tampala has an IC50 value of 104 ppm and can induce an apoptosis effect in MCF-7 cells. This study concludes that the anti-cancer activity of bajakah tampala extract is weak against MCF-7 breast cancer cell lines.