Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Tujuan Mengembangkan cara spot sederhana untuk melekatkan suspensi sel hasil kultur pada kaca objek. Metode Kami membandingkan tiga cara, masing-masing pada kaca objek biasa dan khusus (Shandon-Polysin). Ketiga cara tersebut adalah membuat sediaan spot kecil secara langsung, atau dengan menambahkan 3 atau 10 μl fetal bovine serum (FBS) per 20 μl sampel. Dengan demikian, secara keseluruhan ada 6 cara. Hasilnya dinilai secara kualitatif dalam hal warna latar belakang, warna dan keutuhan batas spot, dan bagian yang terlipat dan terlepas. Selanjutnya, untuk tiap kaca objek, jumlah sel utuh yang melekat dihitung, dan persentase sel utuh yang melekat per jumlah sel yang dibuat spot juga dihitung. Perbedaan sel utuh yang melekat di antara keenam cara di atas dianalisis dengan ANOVA menggunakan program SPSS 13.0 for Windows. Hasil Tidak ada perbedaan bermakna di antara keenam cara di atas dalam hal persentase sel utuh yang melekat (P= 0,804), walaupun cara yang menggunakan kaca objek khusus tanpa penambahan FBS (kadar FBS final 5%) menghasilkan persentase sel utuh melekat yang paling tinggi, dengan latar belakang bersih tanpa lipatan. Kesimpulan Kami telah mengembangkan cara spot sederhana untuk membuat sediaan sitologi suspensi sel hasil kultur, dan hasil terbaik didapat dengan menggunakan kaca objek khusus dengan suspensi sel berkadar FBS 5%.

---

Abstract
Aim To develop a simple spot method to attach cultured cells in suspension on to a glass slide. Methods We compared three approaches using both conventional and special glass slide (Shandon-Polysin)., either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows. Results There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts. Conclusions We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension.

Abstrak :
This review article gives a brief history of the classical experiments that led to the development of the embryo culture medium and in vitro embryo culture. It proposes that, in view of the outstanding and significant pioneering contributions of Wesley Kingston Whitten to the development of embryo culture medium, he be considered the “Father of Embryo Culture Medium”. Furthermore, it describes the nutritional requirements of early embryos and how these requirements with specific references to carbohydrates, amino acids, phosphates, growth factors, etc, have been utilized to formulate increasingly more complex embryo culture media. This has led to the development of progressively more efficacious embryo culture media including the formulation of completely defined and synthetic protein-free embryo culture medium. The review also describes physical factors, growth factors, insemination methods for the fertilization of oocytes and culture methods affecting embryo growth, development, metabolism, oxygen embryotoxicity and survival. In procedural terms, the review also summarizes the evolution of embryo culture techniques from tube culture to, microdrop culture under oil to co-culture to ultra microdrop culture techniques. It includes techniques of in vitro maturation and for the selection of potentially viable embryos of various developmental stages.

Abstrak :
Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability.