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Abstrak :
This paper aims to examine the influence of the minganhak on the formation of knowledge culture in colonial Korea....

Abstrak :
ABSTRAK
Optimalisasi portofolio bertujuan agar investor mendapatkan return tertinggi dan mendapatkan risiko terendah. Untuk mencapai tujuan ini, investor melakukan diversifikasi untuk meningkatkan kinerja portofolio dengan meminimalkan risiko portofolio. Pada penelitian ini digunakan algoritma Iterative K-Means -+ sebagai metode clustering dan Ant Colony Optimization (ACO). Pengelompokan digunakan untuk diversifikasi portofolio berdasarkan rasio keuangan masing-masing saham. K-Means berulang -+ ini memperbaiki solusi dari K-Means dengan menghapus 1 cluster (minus), membagi 1 cluster (plus) dan re-clustering di setiap iterasi. Setelah pengelompokan, beberapa saham dipilih dan bobotnya ditentukan dengan metode metaheuristik, yaitu: Algoritma Ant Colony Optimization (ACO). Hasil numerik dari metode ini dievaluasi dengan data yang sebenarnya.
ABSTRACT
Portfolio optimization aims for investors to get the highest return and get the lowest risk. To achieve this goal, investors diversify to improve portfolio performance by minimizing portfolio risk. In this study, the Iterative K-Means -+ algorithm was used as a clustering method and Ant Colony Optimization (ACO). Grouping is used to diversify the portfolio based on the financial ratios of each stock. Iterative K-Means --+ this improves the solution of K-Means by removing 1 cluster (minus), dividing 1 cluster (plus) and re-clustering in each iteration. After grouping, several stocks are selected and their weights are determined by the metaheuristic method, namely: Ant Colony Optimization (ACO) Algorithm. The numerical results of this method are evaluated with actual data.

Abstrak :
Granulocyte Colony Stimulating Factor (G-CSF) merupakan faktor pertumbuhan hematopoetik yang berfungsi merangsang proliferasi dan diferensiasi neutrofil. Protein G-CSF rekombinan yang dikembangkan dan diproduksi menggunakan sel inang Escherichia coli dan Chinese Hamster Ovary (CHO) masih memiliki kelemahan, sehingga pada penelitian ini dikembangkan suatu produk biosimilar G-CSF rekombinan menggunakan sel inang Pichia pastoris. Fokus penelitian ini adalah memproduksi dan mempurifikasi protein G-CSF rekombinan. Produksi protein rekombinan dilakukan dengan menginduksi kultur menggunakan metanol konsentrasi 0,5% tiap 12 jam dan dilakukan sampling terhadap kultur pada jam ke-0, 12, 24, 36 dan 48. Hasil analisis western blot menunjukkan adanya peningkatan produksi protein rekombinan tiap 12 jam. Protein G-CSF rekombinan dipresipitasi menggunakan amonium sulfat konsentrasi 80%, kemudian didialisis. Konsentrasi protein total diukur dengan spektrofotometer menggunakan metoda Bicinchoninic Acid (BCA). Hasil pengukuran menunjukkan konsentrasi protein total tertinggi adalah sampel protein yang dipresipitasi dengan 80% amonium sulfat. Selanjutnya, purifikasi dilakukan menggunakan teknik kromatografi afinitas dengan resin Ni-NTA. Hasil analisis SDS PAGE menunjukkan protein GCSF rekombinan berukuran 18,5 kDa dan dengan analisis slot blot terdeteksi berwarna ungu.

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Granulocyte Colony Stimulating Factor (G-CSF) is a hematopoietic growth factor that acts to stimulate neutrophilic proliferation and differentiation. Recombinant protein G-CSF developed and produced using cellular host Escherichia coli and Chinese hamster ovary (CHO) still has a weakness, so that in this study we developed a bio similar product of recombinant G-CSF using cellular host Pichia pastoris. The aim of this research was to produce and purify recombinant protein G-CSF. Production of recombinant protein was done by inducing culture with methanol 0.5% every 12 hours and sampling was carried out at 0, 12, 24, 36 and 48 hours. The results of western blot analysis showed an increase the production of recombinant protein every 12 hours. Recombinant protein G-CSF was precipitated using ammonium sulfate 80% of concentration, and then dialyzed. Concentration of total protein was measured by a spectrophotometer using the Bicinchoninic Acid (BCA) method. The measurement results showed the highest concentrations of total protein was present in samples that precipitated with 80% ammonium sulfate. Furthermore, purification performed using affinity chromatography techniques with Ni-NTA resin. The results of SDS PAGE analysis showed the recombinant protein G-CSF sized 18.5 kDa and with a slot blot analysis detected a purple color.

Abstrak :
This milestones in drug therapy volume describes the experience of the last 20 years of treatment with recombinant human G-CSF, including the basic science, the use of recombinant human G-CSF in both the oncology and nononcology settings, and the safety and economics of its use.