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Abstrak :
Cytotoxicity tests on ethanol extract of aglalia elliptica blume leaves and its fractions on cervical cencer cell line (HeLa) were carried out. Dried powder of Aglaia elliptica leaves was extracted using ethanol and then fractionated by n-hrxane, ethyl acetate and buthanol extraxt solvents. The cytotoxicity tets using MTT method (prolipheration inhibition tests on HeLa cell lines were conducted for ethanol extract, n-hexana ethyl acetate and buthanol fractions. The results showed that the ethanol extraxt of aglaia elliptica and its fractions have inhibition effect againts HeLa Cell line with IC50 of 282.44 ppm,177.64 ppm, 16.03 ppm,211.77 ppm, 181.33 ppm. 181.33 for ethanol extracts ,n-hexane(Heal extract , ethyl acceate, buthanol,and water fractions respecively.

Abstrak :
Pembuatan Stable Cell Line sendiri membutuhkan proses pengantaran materi genetik untuk mencapai tahap ekspresi gen rekombinan secara berkelanjutan. Metode ini menggunakan transfeksi untuk membantu mencapai tahapan tersebut. Badan Riset dan Inovasi Nasional (BRIN) Indonesia telah berhasil membuat konstruksi plasmid rekombinan pengekspresi protein Spike SARS-CoV-2. Namun, belum dilakukan penelitian lebih lanjut terkait penggunaan konstruksi plasmid rekombinan tersebut. Oleh karena itu, tujuan penelitian ini adalah untuk memvalidasi ekspresi protein rekombinan dari plasmid rekombinan pengekspresi Spike SARS-CoV-2 yang akan digunakan dalam pembuatan Stable Cell Line pada galur sel mamalia 293T. Validasi ekspresi dari empat protein SARS-CoV-2 (Spike Full, Subunit S1, Subunit S2, dan Receptor Binding Domain) dilakukan melalui metode Immunofluorescence Assay (IFA) dan Western Blot (WB). Hasil menunjukkan bahwa dari empat ragam protein (Spike Full, Subunit S1, Subunit S2, dan Receptor Binding Domain) terbukti fungsional secara ekspresi dan sesuai dengan ukuran protein yang sesuai. Uji IFA menunjukkan bahwa terdapat dua nilai rata-rata Corrected Total Cell Fluorescence (CTCF) yang unggul yaitu pada protein Spike Full dan Subunit S2 (118.813 dan 264.159 CTCF) sel pasca transfeksi yang menandakan bahwa terdapat perbedaan kemampuan ekspresi dari masing-masing protein. Uji western blot telah membuktikan dua protein (Spike Full dan Subunit S2) memiliki ukuran molekul yang sesuai (142,5 dan 66,0 kDa). Sehingga, dapat disimpulkan bahwa plasmid rekombinan yang dikonstruksi oleh BRIN terbukti fungsional dan dapat dilanjutkan ke dalam penggunaannya untuk pembuatan Stable Cell Line. ......Making a Stable Cell Line requires a process of delivering genetic material to reach the continuous recombinant gene expression stage. This method uses transfection to help achieve this stage. The National Research and Innovation Agency of Indonesia (BRIN) has successfully constructed a recombinant plasmid expressing the SARS-CoV-2 Spike protein. However, no further research has been carried out regarding using these recombinant plasmid constructs. Therefore, this study aimed to validate the expression of recombinant proteins from the SARS-CoV-2 Spike-expressing recombinant plasmid to manufacture Stable Cell Line in mammalian cell line 293T. Validation of the expression of four SARS-CoV-2 proteins (Spike Full, Subunit S1, Subunit S2, and Receptor Binding Domain) was carried out using Immunofluorescence Assay (IFA) and Western Blot (WB) methods. The results showed that the four protein variants (Spike Full, S1 Subunit, S2 Subunit, and Receptor Binding Domain) were functional in expression and according to the appropriate protein size. The IFA test showed two superior Corrected Total Cell Fluorescence (CTCF) values: the Spike Full protein and the S2 Subunit (118.813 and 264159 CTCF) of post-transfection cells, which indicated that there were differences in the expression ability of each protein. The Western Blot test has proven that two proteins (Spike Full and Subunit S2) have the appropriate molecular size (142.5 and 66.0 kDa). Thus, it can be concluded that the recombinant plasmid constructed by BRIN is proven to be functional and can be used to manufacture Stable Cell Lines.

Abstrak :
Latar Belakang: Vitamin D telah terbukti memiliki sifat anti kanker sebagai antioksidan, anti-proliferasi, dan diferensiasi sel. Khasiat vitamin D sebagai agen antikanker memicu peneliti untuk mencari tahu apakah vitamin D bermanfaat sebagai radiosensitizer. Berbagai penelitian telah dilakukan pada cell line dalam berbagai jenis kanker, tetapi manfaat vitamin D sebagai radiosensitizer masih kontroversial. Tujuan: Manfaat vitamin D sebagai radiosensitizer masih kontroversial karena beberapa hasil studi yang tidak jelas. Ulasan ini bertujuan untuk menyelidiki pemanfaatan Vitamin D3 (Kalsitriol) sebagai radiosensitizer di berbagai lini sel melalui tinjauan literatur. Bahan/Metode: Pencarian sistematis dari pangkalan data literatur medis yang tersedia dilakukan pada studi in vitro dengan Vitamin D sebagai radiosensitizer di semua jenis cell line. Sebanyak 11 studi in vitro dievaluasi. Hasil: Sembilan studi dalam ulasan ini menunjukkan efek yang signifikan dari Vitamin D sebagai agen radiosensitizer dengan meningkatkan autofagi sitotoksik, meningkatkan apoptosis, menghambat kesintasan sel dan gen pemicu proliferasi sel dengan menghambat ReIB, dan menginduksi senesens. Dua penelitian lainnya menunjukkan tidak ada efek yang signifikan dalam mekanisme radiosensitisasi vitamin D karena kurangnya bukti dalam lingkungan in vitro. Kesimpulan: Vitamin D memiliki sifat antikanker dan dapat digunakan sebagai radiosensitizer dengan berbagai mekanisme di berbagai cell line. Penelitian lebih lanjut terutama dalam pengaturan in vivo perlu dievaluasi. ......Background: Vitamin D has been shown to have anti-cancer properties as antioxidants, anti-proliferative, and cell differentiation. The property of vitamin D as an anticancer agent triggers researchers to find out whether vitamin D is useful as a radiosensitizer. Multiple studies have been carried out on cell lines in various types of cancer, but the benefits of vitamin D as a radiosensitizer are still become controversial. Purpose: The benefits of vitamin D as a radiosensitizer are still controversial due to some incoherent study results. This review aim to investigate the utilization of Vitamin D3 (Calcitriol) as radiosensitizer in various cell line through review of literature. Materials/Methods: A systematic search of available medical literature databases was performed on in vitro studies with Vitamin D as radiosensitizer in all types of cell line. A total of 11 in vitro studies were evaluated. Results: Nine studies in this review showed significant effect of Vitamin D as radiosensitizer agent by promoting cytotoxic autofagi, increasing apoptosis, inhibition of cell survival and proliferation promoting gene in ReIB inhibition, and inducing senescenes. The two remaining studies showed no significant effect in radiosensitizing mechanism of Vitamin D due to lack of evidence in vitro settings. Conclusion: Vitamin D have anticancer property and can be used as radiosensitizer by imploring various mechanism pathways in various cell line. Further research especially in vivo setting need to be evaluated.