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Abstrak :
Candida glabrata adalah spesies kedua yang terisolasi pada kandidiasis oral setelah Candida albicans. Nigella sativa (jintan hitam) dilaporkan memiliki efek antijamur. Tujuan penelitian ini adalah menganalisis efek antijamur ekstrak biji Nigella sativa terhadap viabilitas Candida glabrata. Candida glabrata ditambahkan ke dalam 96 microwell plate yang telah dilapisi saliva buatan kemudian dipajankan dengan ekstrak biji Nigella sativa 6.25%-50% dan amphotericin B 250 mg/ml. Dilakukan pengujian viabilitas Candida glabrata dengan uji MTT. Viabilitas Candida glabrata lebih rendah setelah pamajanan dengan ekstrak biji Nigella sativa dibandingkan kontrol negatif. Viabilitas Candida glabrata semakin menurun seiring dengan peningkatan konsentrasi ekstrak biji Nigella sativa. ......Candida glabrata is the second most frequently isolated species from Oral Candidiasis, after Candida albicans. Nigella sativa (black cumin) is reported has antifungal effect. This research was to analyze Nigella sativa antifungal effect Seeds’ extract to the Candida glabrata viability. Candida glabrata was added on 96-microwell plate that had been coated with artificial saliva and exposed to 6.25-50% Nigella sativa seeds’ extract and amphotericin B 250 mg/ml. Candida glabrata viability was determined by MTT assay. Candida glabrata viability was lower after exposed by Nigella sativa seeds’ extract than negative control. Candida glabrata viability was decreased with increasing Nigella sativa seeds’ extract concentrations.

Abstrak :
The effects of bovine lactoferrin (bLF) on the growth of Candida species and on inflammatory cytokine production in gingival keratinocytes, NDUSD-1 co-cultured with Candida strains were investigated. The results showed that bLF at 10 and 100 μg/mL significantly inhibits the growth of two C. albicans strains and two C. glabrata strains isolated from the saliva of elderly people requiring nursing care, respectively. The levels of inflammatory cytokines, interleukin (IL)-6, and IL-8 in NDUSD-1 co-cultured with each of these four Candida strains were measured. C. albicans tend to have a more potent capacity than C. glabrata to induce the production of the inflammatory cytokines in NDUSD-1. The levels of IL-6 and IL-8 in NDUSD-1 co-cultured with each of Candida species were measured after addition of bLF. bLF at concentrations from 1 to 100 μg/mL significantly inhibited the production of these cytokines in NDUSD-1 co-cultured with Candida species. These findings suggest that bLF may be useful in reducing the risk of aspiration pneumonia among elderly people requiring care for whom oral care is difficult.