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Abstrak :
Latar Belakang: Metilasi di area promoter berpotensi mengakibatkan gene silencing pada gen CDH1 yang berperan penting dalam adhesi antarsel dan morfogenesis kraniofasial. Tujuan: Mengetahui distribusi metilasi antara individu cleft dan non-cleft. Metode: 24 sampel DNA penderita orofacial cleft dan 24 sampel kontrol dianalisis menggunakan teknik methylation-specific PCR (MSP). Hasil: Dari kelompok cleft didapatkan 5 sampel (20,83%) berstatus fully methylated dan 19 sampel (79,17%) berstatus partially methylated, sedangkan dari kelompok kontrol didapatkan 24 sampel (100%) berstatus partially methylated. Kesimpulan: Terjadi metilasi CDH1 pada penderita orofacial cleft, namun secara statistik tidak terdapat perbedaan bermakna pada distribusi status metilasi CDH1 antara individu cleft dan non-cleft (p=0,05).

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Background: Methylation at promoter area potentially results in silencing of CDH1 gene which plays important role in cell adhesion and craniofacial morphogenesis. Objective: To obtain the distribution of CDH1 methylation in cleft and non-cleft individuals. Methods: 24 DNA samples of individuals with orofacial cleft and 24 control samples were analyzed with methylation-specific PCR (MSP) technique. Results: From cleft group, 5 (20.83%) were fully methylated and 19 (79.17%) were partially methylated; while from control group, 24 (100%) were partially methylated. Conclusion: CDH1 methylation was observed in orofacial cleft affected individuals but there is no significant difference in CDH1 methylation status between cleft and non-cleft individuals (p=0.05).

Abstrak :
In this volume, a group of internationally recognized experts reviews AJ biology over a wide range of organization, from atoms to molecules, to protein complexes, molecular networks, cells, tissues, and overall animal development. AJs have also been an integral part of animal evolution, and play central roles in cancer development, pathogen infection and other diseases. This book addresses major questions encompassing AJ biology. How did AJs evolve? How do cadherins and catenins interact to assemble AJs and mediate adhesion? How do AJs interface with other cellular machinery to couple adhesion with the whole cell? How do AJs affect cell behaviour and multicellular development? How can abnormal AJ activity lead to disease?