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"Latar Belakang: Pasien adenokarsinoma prostat sering kali datang dalam kondisi telah lanjut atau bermetastasis. Terapi yang dapat diberikan pada kasus adenokarsinoma stadium lanjut umumnya pengobatan androgen deprivation therapy (ADT), namun sebagian pasien mengalami castration-resistant prostate cancer (CRPC). Jalur inhibitor immune check point dapat menjadi alternatif pilihan terapi. Tujuan penelitian ini adalah untuk mengetahui korelasi ekspresi PD-L1 dengan gambaran histopatologik dan gejala klinikopatologis adenokarsinoma prostat.Metode: Penelitian ini merupakan penelitian analitik korelasi terhadap kasus-kasus adenokarsinoma prostat retrospektif. Dilakukan pulasan imunohistokimia PD-L1 klon 22C3 terhadap 30 subjek dan dinilai menggunakan combined proportion score (CPS). dilakukan penilaian ulang skor Gleason dan WHO/ISUP grade, serta penilaian tumor infiltrating lymphocytes (TILS). Dilakukan uji korelasi menggunakan SPSS 20.0 untuk melihat korelasi dengan derajat WHO/ISUP, TILS, nilai PSA dan body mass indek (BMI).Hasil: Dari 30 sampel yang dipilih secara acak didapatkan rata-rata usia 63 tahun. Body mass index antara 17,6 hingga 35,2. Nilai PSA antara 4,1 ng/ml hingga 973 ng/ml. Tidak terdapat korelasi antara ekspresi PD-L1 dengan derajat WHO/ISUP, BMI dan nilai PSA. Didapatkan korelasi dengan intratumoral TILS tapi tidak pada Stromal TILS.Diskusi: Pada penelitian ini didapatkan korelasi bermakna dengan intratumoral TILS, namun sebagian besar penelitian lain pada umumnya menggunakan hanya stromal TILS. Dibutuhkan penelitian lebih lanjut untuk diaplikasikan secara klinis.

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Background: Prostate adenocarcinoma patients often present in an advanced or metastatic condition. Treatment that can be given in cases of advanced adenocarcinoma is androgen deprivation therapy (ADT), but some patients experience castration-resistant prostate cancer (CRPC). Immune check point inhibitor pathways can be an alternative treatment option. The aim

of this study was to determine the correlation of PD-L1 expression with histopathologic features and clinicopathological symptoms of prostate adenocarcinoma.

Methods: This study is a retrospective correlation analytic study of cases of prostate adenocarcinoma. 30 subjects were performed with PD-L1 clone 22C3 immunohistochemistry and assessed using a combined proportion score (CPS). a reassessment of the Gleason score and WHO/ISUP grade, as well as an assessment of tumor infiltrating lymphocytes (TILS). Correlation test was performed using SPSS 20.0 to see the correlation with WHO/ISUP degrees, TILS, PSA values and body mass index (BMI).

Results: From 30 randomly selected subjects, the mean age was 63 years. Body mass index between 17.6 and 35.2. PSA values are between 4.1 ng/ml to 973 ng/ml. There was no correlation between PD-L1 expression and WHO / ISUP degree, BMI and PSA value. There was a correlation

with intratumoral TILS but not Stromal TILS,

Discussion: PD-L1 expression with TILS has been shown to be associated with various tumors. In this study, there was a significant correlation with intratumoral TILS, but most other studies generally used only stromal TILS. Further research is needed for its clinical applicatio."

"Latar Belakang: Karsinoma nasofaring (KNF) adalah karsinoma yang berasal dari epitel permukaan nasofaring dengan angka insidensi yang tinggi di Tiongkok dan Asia Selatan. KNF masih menjadi masalah kesehatan di Indonesia dan prognosisnya dilaporkan buruk terkait dengan penanganan yang sering tidak optimal karena kebanyakan (60-95%) pasien berobat dalam stadium lanjut. Saat ini berkembang penelitian terhadap tumor microenvironment yang dapat dinilai melalui tumor infiltrating lympochyte (TIL) yang berkaitan dengan respons terapi pada beberapa tumor, termasuk KNF. Beberapa penelitian menyebutkan bahwa TIL salah satunya dapat dinilai dengan Foxp3. Foxp3 diketahui sebagai penanda sel T regulator (Treg) yang turut berperan dalam immunoregulator lingkungan sel-sel tumor dan dapat digunakan sebagai salah satu faktor prognosis. Hubungan antara ekspresi Foxp3 dengan respons terapi dapat dipertimbangkan menjadi salah satu faktor yang mempengaruhi prognosis KNF.Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan antara ekspresi Foxp3 dengan respons terapi karsinoma nasofaring.Metode: Penelitian analitik dengan desain potong lintang pada sediaan KNF tidak berkeratin di Departemen Patologi Anatomik FKUI/RSCM selama periode Januari 2018 hingga Desember 2020. Pengambilan sampel penelitian dilakukan secara consecutive sampling dari kasus yang memenuhi kriteria inklusi dan eksklusi sesuai perhitungan besar sampel untuk masing-masing kelompok. Pemeriksaan imunohistokimia menggunakan antibodi primer monoklonal Foxp3. Data imunoekspresi dianalisis untuk mengetahui hubungannya dengan respons terapi karsinoma nasofaring.Hasil: Dari 60 kasus yang terdiagnosis KNF, sebanyak 40 kasus (66,7%) berjenis kelamin laki-laki dan 20 kasus lainnya (33,3%) berjenis kelamin perempuan dengan rasio 2:1. Terdapat perbedaan bermakna ekspresi Foxp3 intratumoral dengan respons terapi (p=0,01). Tidak terdapat perbedaan bermakna ekspresi Foxp3 peritumoral dengan respons terapi (p=0,114).Kesimpulan: Ekspresi Foxp3 mempunyai hubungan yang bermakna secara statistik dengan hasil evaluasi respons pasca kemoradiasi karsinoma nasofaring.

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Background: Nasopharyngeal carcinoma (NPC) is a carcinoma originating from the surface epithelium of the nasopharynx with a high incidence in Tiongkok and South Asia. NPC still become main health issue in Indonesia and the prognosis is reported to be poor due to suboptimal treatment because most of the patients (60-95%) are treated at an advanced stage. Currently, many research are developing on the tumor microenvironment that can be assessed by tumor infiltrating lymphochyte (TIL) which is associated with the treatment response in several tumors, including NPC. Some studies explore that TIL can be assessed with Foxp3. Foxp3 is known as a regulatory T cell (Treg) marker that plays a role in the immunoregulator environment of tumor cells and can be used as a prognostic factor. The relationship between Foxp3 expression and treatment response can be considered as one of the factors that influence the prognosis of NPC.

Aims: This study aims to determine the relationship between Foxp3 expression and treatment response of NPC.

Methods: An analytical study with a cross-sectional design on non-keratinizing NPC diagnosed at Anatomical Pathology Department of FKUI/RSCM during January 2018 until December 2020. The research sample was taken by consecutive sampling of cases that met the inclusion and did not include the exclusion criteria according to the calculation of the sample size for each group. Immunohistochemical examination using Foxp3 monoclonal antibody. Immunoexpression data were analyzed to determine its relationship with the treatment response of NPC.

Results: From 60 selected cases diagnosed with NPC, there were consisted of 40 male patients (66,7%) and 20 female patients (33,3%) with ratio 2:1. There was a significant difference in intratumoral Foxp3 expression with treatment response (p=0.01). There was no significant difference in peritumoral Foxp3 expression with treatment response (p=0.114).

Conclusion: Foxp3 expression had a statistically significant relationship with response therapy after chemoradiation."

"Latar Belakang: Karsinoma sel skuamosa laring (KSSL) dengan metastasis ke kelenjar getah bening (KGB) leher memiliki angka kelangsungan hidup 5 tahun kurang dari 50%. Banyak penelitian menunjukkan bahwa MMP-9 dan CCR7 berhubungan dengan metastasis ke KGB leher. Overekspresi MMP-9 dan CCR7 berhubungan dengan sifat sel tumor yang lebih agresif dan prognosis yang lebih buruk dikarenakan cenderung bermetastasis ke KGB leher.Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan ekspresi MMP-9 dan CCR7 pada KSSL yang bermetastasis dan tidak metastasis ke KGB leher.Metode: Penelitian analitik observasional dengan desain potong lintang pada sediaan operasi laringektomi parsial maupun total dengan diseksi leher di RSCM periode Desember 2017 sampai Desember 2019. Sampel penelitian dibagi menjadi 2 kelompok, yaitu KSSL dengan metastasis KGB leher dan tanpa metastasis. Pengambilan sampel penelitian dilakukan secara konsekutif dari kasus yang memenuhi kriteria inklusi dan eksklusi sesuai perhitungan besar sampel untuk masing-masing kelompok. Pemeriksaan imunohistokimia menggunakan antibodi primer MMP-9 dan CCR7. Data imunoekspresi dianalisis untuk mengetahui hubungannya dengan terjadinya metastasis ke KGB leher.Hasil: Kejadian metastasis KSSL ke KGB leher berhubungan dengan ekspresi MMP-9 (P<0,05). Ditemukan ekspresi MMP-9 yang tinggi pada KSSL-M sebesar 41,7% sedangkan KSSL-NM 8,3%. Ekspresi CCR7 juga berkorelasi dengan metastasis KSSL ke KGB leher (P<0,05), ekspresi CCR7 yang tinggi pada KSSL-M sebanyak 48,3% sedangkan KSSL-NM hanya 10%. Ditemukan pula hubungan antara ekspresi MMP-9 dan CCR7 dengan terjadinya metastasis KSSL ke KGB (p=0,001).Kesimpulan: Metastasis KSSL ke KGB berhubungan dengan ekspresi MMP-9 dan CCR7. Terdapat adanya hubungan antara ekspresi MMP-9 dan CCR7 pada kejadian metastasis KSSL ke KGB leher.

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Background: Laryngeal squamous cell carcinoma (LSCC) with metastasis to the lymph nodes of the neck has a 5-year survival rate of less than 50%. Many studies have shown that MMP-9 and CCR7 are associated with metastasis to cervical lymph nodes. Overexpression of MMP-9 and CCR7 are associated with a more aggressive cell tumour and poor prognosis because they are more likely to metastasis to cervical lymph nodes.

Purpose: This study aims to determine the expression of MMP-9 and CCR7 in metastasis and non-metastatis KSSL to cervical lymph nodes.

Methodology: An observational analytic study with a cross-sectional design on partial and total laryngectomy surgery preparations with neck dissection at RSCM for the period December 2017 to December 2019. The study sample was divided into 2 groups, namely KSSL with cervical lymph nodes metastasis and without metastasis. The research sample was taken by consecutive sampling from cases that met the inclusion and exclusion criteria according to the calculation of the sample for each group. Immunohistochemical examination using primary antibodies MMP-9 and CCR7. Immunoexpression data were analyzed to determine their relationship with metastasis to cervical lymph nodes.

Results: The incidence of SCC metastases to cervical lymph nodes was associated with MMP-9 expression (P<0.05). High MMP-9 expression was found in KSSL-M by 41.7% while in KSSL-NM 8.3%. CCR7 expression also correlated with KSSL metastases to cervical lymph nodes (P<0.05), high CCR7 expression in KSSL-M was 48.3% while KSSL-NM was only 10%. It was also found that there was a relationship between the expression of MMP-9 and CCR7 with the occurrence of SLCC to lymph node metastasis (p=0.001).

Conclusion: Metastasis SLCC to lymph node was associated with MMP-9 and CCR7 expression. There is a relationship between the expression of MMP-9 and CCR7 in the incidence of SCC metastases to cervical lymph nodes."