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ABSTRAK Pendahuluan. Osteoarthritis (OA) adalah sebuah penyakit sendi degeneratif yang
menyebabkan disabilitas dengan prevalensi yang terus meningkat. Hormon
pertumbuhan memiliki efek regenerasi tulang rawan secara langsung melalui
stimulasi sel kondroblas dan proses morphoangiogenesis juga melalui faktor
pertumbuhan secara sistemik. Penelitian ini bertujuan untuk mengetahui manfaat
suntikan sendi dengan hormon pertumbuhan pada kasus Osteoarthritis.
Metode Penelitian. Penelitian dilakukan di Rumah Sakit Hewan Institut
Pertanian Bogor pada bulan Mei hingga September 2015. Desain penelitian adalah
randomized post test only control group. Sejumlah 21 ekor kelinci Selandia Baru
putih, berat 1.9-2.6kg, usia 7-8 bulan. Kelinci dibagi secara acak menjadi
kelompok kontrol (NaCl 0.9%), suntikan hormon pertumbuhan (4iu), dan suntikan
asam hyaluronat (6mg) . Dengan metode acak tersamar dilakukan suntikan
kolagenase tipe II C. Histolyticum pada hari 1 dan ke 4 pada lutut kiri, kemudian
tindakan penyuntikan dilakukan sebanyak tiga kali dengan selang waktu 1
minggu. Evaluasi dengan periode kepincangan, skoring makroskopis, histologis
dilakukan pada minggu ke-8 pasca penyuntikan pertama.
Temuan Penelitian. Berdasarkan hasil penelitian ditemukan periode kepincangan
pada grup yang diberikan hormon pertumbuhan lebih singkat, dan bermakna
secara statistik dibandingkan dengan grup kontrol (p<0.001), grup asam
hyaluronat (p<0.03), dan grup hormon pertumbuhan (p<0.001). Evaluasi skor
makroskopik dengan skor yoshimi menunjukan bahwa kelompok hormon
pertumbuhan memiliki kerusakan tulang rawan yang lebih ringan jika
dibandingkan dengan grup kontrol (p=0.001) dan grup asam hyaluronat (p=0.04).
Skoring histopatologis menggunakan skor modifikasi Mankin menunjukan pada
kelompok dengan hormon pertumbuhan memiliki angka terendah dibandingkan
grup lainnya (p=0.001), grup kontrol (p=0.001), grup asam hyaluronat (p=0.015).
Kesimpulan. Suntikan hormon pertumbuhan ke dalam sendi memiliki efektifitas
yang lebih baik dibandingkan dengan Asam hyaluronat pada model osteoarthritis.
Hormon pertumbuhan memberikan harapan baru sebagai alternatif dalam terapi
osteoarthritis.
ABSTRACT Introduction. Osteoarthritis is a degenerative joint disorder that cause disability
for patients all over the globe, with an increasing number of patients. Growth
hormone (GH) works trough direct and indirect effect on cartilage regeneration by
chondroblast stimulation, stimulation of growth factors and morphoangiogenesis
process. Further research is needed to know the effects of intra articular joint
injection of growth hormone using validated animal model and reliable outcome
measurement.
Methods. This study was conducted in Animal Hospital of Agricultural Institute
Bogor west Java, from May to September 2015. The design of the study was
randomized posttest only control group. Male white New Zealand rabbit (n=21)
weighted 1.9-2.6kg, age 6-7months were used in this study. The sample was
randomized and divided into three groups. All groups recieved intra articular
injection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1
and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline
(0.6ml) were done at 2 weeks after collagenase injection once a week for
consecutive 3 weeks. Evaluation of weight and lameness periode is done
periodically, histopathological and macroscopic score were done at 8 weeks since
the first injection.
Result. The lameness priode for control group is significantly longer than both of
the experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001).
Macroscopic score evaluation taken from the lateral condyle of the left femur
showed that the GH group received significantly less cartilage damage than the
HA group (P=0.04) and placebo (P=0.01). Histopathological score was also found
lowest at the GH group (p=0.001), with significant difference in control
(p=0.001), and HA group (p=0.015).).
Conclusion. Intraarticular injection of growth hormone is found to be more
effective compared to hyaluronic acid on rabbit osteoarthritis model. This results
showed promising result for intra articular injection of GH as an alternative
treatment for osteoarthritis.;Introduction. Osteoarthritis is a degenerative joint disorder that cause disability
for patients all over the globe, with an increasing number of patients. Growth
hormone (GH) works trough direct and indirect effect on cartilage regeneration by
chondroblast stimulation, stimulation of growth factors and morphoangiogenesis
process. Further research is needed to know the effects of intra articular joint
injection of growth hormone using validated animal model and reliable outcome
measurement.
Methods. This study was conducted in Animal Hospital of Agricultural Institute
Bogor west Java, from May to September 2015. The design of the study was
randomized posttest only control group. Male white New Zealand rabbit (n=21)
weighted 1.9-2.6kg, age 6-7months were used in this study. The sample was
randomized and divided into three groups. All groups recieved intra articular
injection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1
and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline
(0.6ml) were done at 2 weeks after collagenase injection once a week for
consecutive 3 weeks. Evaluation of weight and lameness periode is done
periodically, histopathological and macroscopic score were done at 8 weeks since
the first injection.
Result. The lameness priode for control group is significantly longer than both of
the experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001).
Macroscopic score evaluation taken from the lateral condyle of the left femur
showed that the GH group received significantly less cartilage damage than the
HA group (P=0.04) and placebo (P=0.01). Histopathological score was also found
lowest at the GH group (p=0.001), with significant difference in control
(p=0.001), and HA group (p=0.015).).
Conclusion. Intraarticular injection of growth hormone is found to be more
effective compared to hyaluronic acid on rabbit osteoarthritis model. This results
showed promising result for intra articular injection of GH as an alternative
treatment for osteoarthritis.;Introduction. Osteoarthritis is a degenerative joint disorder that cause disability
for patients all over the globe, with an increasing number of patients. Growth
hormone (GH) works trough direct and indirect effect on cartilage regeneration by
chondroblast stimulation, stimulation of growth factors and morphoangiogenesis
process. Further research is needed to know the effects of intra articular joint
injection of growth hormone using validated animal model and reliable outcome
measurement.
Methods. This study was conducted in Animal Hospital of Agricultural Institute
Bogor west Java, from May to September 2015. The design of the study was
randomized posttest only control group. Male white New Zealand rabbit (n=21)
weighted 1.9-2.6kg, age 6-7months were used in this study. The sample was
randomized and divided into three groups. All groups recieved intra articular
injection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1
and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline
(0.6ml) were done at 2 weeks after collagenase injection once a week for
consecutive 3 weeks. Evaluation of weight and lameness periode is done
periodically, histopathological and macroscopic score were done at 8 weeks since
the first injection.
Result. The lameness priode for control group is significantly longer than both of
the experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001).
Macroscopic score evaluation taken from the lateral condyle of the left femur
showed that the GH group received significantly less cartilage damage than the
HA group (P=0.04) and placebo (P=0.01). Histopathological score was also found
lowest at the GH group (p=0.001), with significant difference in control
(p=0.001), and HA group (p=0.015).).
Conclusion. Intraarticular injection of growth hormone is found to be more
effective compared to hyaluronic acid on rabbit osteoarthritis model. This results
showed promising result for intra articular injection of GH as an alternative
treatment for osteoarthritis."
