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Eldia Anggidenia
Potensi Makroalga Alami Sargassum polycystum dan Makroalga Budidaya Eucheuma cottonii dalam Menyerap dan Menyimpan Karbon serta Nutrien di Pulau Panjang, Teluk Banten = The Potency of Wild Macroalgae Sargassum polycystum and Cultivation Macroalgae Eucheuma cottonii to Absorb and Store Carbon also Nutrient in Panjang Island, Banten Bay
"[Penelitian mengenai potensi makroalga alami Sargassum polycystum
dan makroalga budidaya Eucheuma cottonii dalam menyerap dan menyimpan
karbon serta nutrien di Pulau Panjang, Teluk Banten telah dilakukan pada bulan
Oktober hingga November 2014. Penelitian bertujuan untuk mengetahui laju
penyerapan karbon, kandungan nutrien dan produktivitas primer dari makroalga S.
polycystum dan E. cottonii. Lokasi penelitian terletak di bagian hamparan gosong
karang dan berlumpur dengan kedalaman 0,5-5 m. Pengamatan pertumbuhan dan
laju penyerapan karbon menggunakan metode penandaan thallus pada 30 sampel
makroalga setiap hari selama 7 hari. Sampel makroalga selanjutnya dianalisis
kandungan nutriennya. Hasil penelitian didapatkan estimasi laju penyerapan
karbon S. polycystum dan E. cottonii adalah 0,0081 gC/hari dan 0,0083 gC/hari.
Kandungan karbon, nitrogen dan fosfat di S. polycystum adalah sebanyak 6,84%,
1,72% dan 0,009% sedangkan kandungan karbon, nitrogen dan fosfat di E.
cottonii adalah 5,99%, 0,67% dan 0,006%. Berdasarkan analisis statistik dengan
uji t, terdapat perbedaan yang signifikan pada laju pertumbuhan, kandungan
nitrogen dan fosfat S. polycystum dengan E. cottonii. Sedangkan untuk kandungan
karbon tidak terdapat perbedaan yang signifikan antara S. polycystum dengan E.
cottonii. Selanjutnya, uji korelasi didapatkan bahwa adanya korelasi antara laju
pertumbuhan S. polycystum dan E. cottonii dengan kandungan nitrogen masingmasing
sedangkan antara laju pertumbuhan dengan kandungan karbon dan fosfat
tidak terdapat korelasi. Produktivitas primer diukur dengan metode botol terang
dan botol gelap yang dimodifikasi. Pengukuran produktivitas primer dilakukan
pada kedalaman 0,5 m untuk S. polycystum dan 0,1 m untuk E. cottonii.
Kandungan oksigen terlarut diukur dengan DO meter. Hasil penelitian didapatkan
rata-rata produktivitas primer S. polycystum sebesar 10,259 ± 3,385 mgC/g/hari
dan E. cottonii sebesar 7,757 ± 4,398 mgC/g/hari.;The research about the potency of wild macroalgae Sargassum polycystum
and cultivation macroalgae Eucheuma cottonii to absorb and store carbon also
nutrient in Panjang Island, Banten Bay was held on October until November 2014.
The research was aimed to estimate carbon sequestration, nutrient content and
primary productivity from macroalgae S. polycystum and E. cottonii. Location of
the research in the stretch of reef and muddy with a depth of 0,5-5 m. The
observation of growth rate and carbon sequestration used thallus marking method
in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its
nutrient content. The results showed that estimation carbon sequestration by S.
polycystum and E. cottonii were 0,0081 gC/day and 0,0083 gC/day. The content
of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%,
1,72% and 0,009% respectively while the content of carbon, nitrogent and
phosphor for Macroalgae E. cottonii were 5,99%, 0,67% and 0,006% respectively.
Based on statistical analysis by t test, there was found significant difference on the
growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii.
While for the carbon content, there was no significant difference between S.
polycystum with E. cottonii. Furthermore, correlation test showed that there was a
correlation between the growth rate of S. polycystum and E. cottonii with
nitrogent content respectively while between the growth rate with carbon and
phosphor content, there was no correlation. Primary productivity were measured
by the light and dark bottle method with modification. Measurement of primary
productivity was held at a depth of 0,5 m from the surface for S. polycystum and
0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO
meter. The result showed that mean of primary productivity for S. polycystum was
10,259 ± 3,385 mgC/g/day and E. cottonii was 7,757 ± 4,398 mgC/g/day;The research about the potency of wild macroalgae Sargassum polycystum
and cultivation macroalgae Eucheuma cottonii to absorb and store carbon also
nutrient in Panjang Island, Banten Bay was held on October until November 2014.
The research was aimed to estimate carbon sequestration, nutrient content and
primary productivity from macroalgae S. polycystum and E. cottonii. Location of
the research in the stretch of reef and muddy with a depth of 0,5-5 m. The
observation of growth rate and carbon sequestration used thallus marking method
in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its
nutrient content. The results showed that estimation carbon sequestration by S.
polycystum and E. cottonii were 0,0081 gC/day and 0,0083 gC/day. The content
of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%,
1,72% and 0,009% respectively while the content of carbon, nitrogent and
phosphor for Macroalgae E. cottonii were 5,99%, 0,67% and 0,006% respectively.
Based on statistical analysis by t test, there was found significant difference on the
growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii.
While for the carbon content, there was no significant difference between S.
polycystum with E. cottonii. Furthermore, correlation test showed that there was a
correlation between the growth rate of S. polycystum and E. cottonii with
nitrogent content respectively while between the growth rate with carbon and
phosphor content, there was no correlation. Primary productivity were measured
by the light and dark bottle method with modification. Measurement of primary
productivity was held at a depth of 0,5 m from the surface for S. polycystum and
0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO
meter. The result showed that mean of primary productivity for S. polycystum was
10,259 ± 3,385 mgC/g/day and E. cottonii was 7,757 ± 4,398 mgC/g/day;The research about the potency of wild macroalgae Sargassum polycystum
and cultivation macroalgae Eucheuma cottonii to absorb and store carbon also
nutrient in Panjang Island, Banten Bay was held on October until November 2014.
The research was aimed to estimate carbon sequestration, nutrient content and
primary productivity from macroalgae S. polycystum and E. cottonii. Location of
the research in the stretch of reef and muddy with a depth of 0,5-5 m. The
observation of growth rate and carbon sequestration used thallus marking method
in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its
nutrient content. The results showed that estimation carbon sequestration by S.
polycystum and E. cottonii were 0,0081 gC/day and 0,0083 gC/day. The content
of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%,
1,72% and 0,009% respectively while the content of carbon, nitrogent and
phosphor for Macroalgae E. cottonii were 5,99%, 0,67% and 0,006% respectively.
Based on statistical analysis by t test, there was found significant difference on the
growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii.
While for the carbon content, there was no significant difference between S.
polycystum with E. cottonii. Furthermore, correlation test showed that there was a
correlation between the growth rate of S. polycystum and E. cottonii with
nitrogent content respectively while between the growth rate with carbon and
phosphor content, there was no correlation. Primary productivity were measured
by the light and dark bottle method with modification. Measurement of primary
productivity was held at a depth of 0,5 m from the surface for S. polycystum and
0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO
meter. The result showed that mean of primary productivity for S. polycystum was
10,259 ± 3,385 mgC/g/day and E. cottonii was 7,757 ± 4,398 mgC/g/day;The research about the potency of wild macroalgae Sargassum polycystum
and cultivation macroalgae Eucheuma cottonii to absorb and store carbon also
nutrient in Panjang Island, Banten Bay was held on October until November 2014.
The research was aimed to estimate carbon sequestration, nutrient content and
primary productivity from macroalgae S. polycystum and E. cottonii. Location of
the research in the stretch of reef and muddy with a depth of 0,5-5 m. The
observation of growth rate and carbon sequestration used thallus marking method
in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its
nutrient content. The results showed that estimation carbon sequestration by S.
polycystum and E. cottonii were 0,0081 gC/day and 0,0083 gC/day. The content
of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%,
1,72% and 0,009% respectively while the content of carbon, nitrogent and
phosphor for Macroalgae E. cottonii were 5,99%, 0,67% and 0,006% respectively.
Based on statistical analysis by t test, there was found significant difference on the
growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii.
While for the carbon content, there was no significant difference between S.
polycystum with E. cottonii. Furthermore, correlation test showed that there was a
correlation between the growth rate of S. polycystum and E. cottonii with
nitrogent content respectively while between the growth rate with carbon and
phosphor content, there was no correlation. Primary productivity were measured
by the light and dark bottle method with modification. Measurement of primary
productivity was held at a depth of 0,5 m from the surface for S. polycystum and
0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO
meter. The result showed that mean of primary productivity for S. polycystum was
10,259 ± 3,385 mgC/g/day and E. cottonii was 7,757 ± 4,398 mgC/g/day;The research about the potency of wild macroalgae Sargassum polycystum
and cultivation macroalgae Eucheuma cottonii to absorb and store carbon also
nutrient in Panjang Island, Banten Bay was held on October until November 2014.
The research was aimed to estimate carbon sequestration, nutrient content and
primary productivity from macroalgae S. polycystum and E. cottonii. Location of
the research in the stretch of reef and muddy with a depth of 0,5-5 m. The
observation of growth rate and carbon sequestration used thallus marking method
in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its
nutrient content. The results showed that estimation carbon sequestration by S.
polycystum and E. cottonii were 0,0081 gC/day and 0,0083 gC/day. The content
of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%,
1,72% and 0,009% respectively while the content of carbon, nitrogent and
phosphor for Macroalgae E. cottonii were 5,99%, 0,67% and 0,006% respectively.
Based on statistical analysis by t test, there was found significant difference on the
growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii.
While for the carbon content, there was no significant difference between S.
polycystum with E. cottonii. Furthermore, correlation test showed that there was a
correlation between the growth rate of S. polycystum and E. cottonii with
nitrogent content respectively while between the growth rate with carbon and
phosphor content, there was no correlation. Primary productivity were measured
by the light and dark bottle method with modification. Measurement of primary
productivity was held at a depth of 0,5 m from the surface for S. polycystum and
0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO
meter. The result showed that mean of primary productivity for S. polycystum was
10,259 ± 3,385 mgC/g/day and E. cottonii was 7,757 ± 4,398 mgC/g/day, The research about the potency of wild macroalgae Sargassum polycystum
and cultivation macroalgae Eucheuma cottonii to absorb and store carbon also
nutrient in Panjang Island, Banten Bay was held on October until November 2014.
The research was aimed to estimate carbon sequestration, nutrient content and
primary productivity from macroalgae S. polycystum and E. cottonii. Location of
the research in the stretch of reef and muddy with a depth of 0,5-5 m. The
observation of growth rate and carbon sequestration used thallus marking method
in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its
nutrient content. The results showed that estimation carbon sequestration by S.
polycystum and E. cottonii were 0,0081 gC/day and 0,0083 gC/day. The content
of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%,
1,72% and 0,009% respectively while the content of carbon, nitrogent and
phosphor for Macroalgae E. cottonii were 5,99%, 0,67% and 0,006% respectively.
Based on statistical analysis by t test, there was found significant difference on the
growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii.
While for the carbon content, there was no significant difference between S.
polycystum with E. cottonii. Furthermore, correlation test showed that there was a
correlation between the growth rate of S. polycystum and E. cottonii with
nitrogent content respectively while between the growth rate with carbon and
phosphor content, there was no correlation. Primary productivity were measured
by the light and dark bottle method with modification. Measurement of primary
productivity was held at a depth of 0,5 m from the surface for S. polycystum and
0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO
meter. The result showed that mean of primary productivity for S. polycystum was
10,259 ± 3,385 mgC/g/day and E. cottonii was 7,757 ± 4,398 mgC/g/day]"
2015
T43623
UI - Tesis Membership  Universitas Indonesia Library
cover
I Gusti Ayu Rai Sawitri
Ekologi pekarangan di Desa Pakraman Kabupaten Buleleng Bali = Ecology homegardens in Pakraman Villages Buleleng Regency Bali
"Pola pekarangan masyarakat desa Pakraman di Bali, didasari atas konsep Tri Hita Karana. Konsep tersebut mengatur ruang pekarangan untuk menciptakan hubungan yang harmonis dengan sang pencipta (Parahyangan), manusia (Pawongan) dan lingkungan (Palemahan). Penelitian ekologi pekarangan dilakukan di desa Pakraman, Buleleng Bali bertujuan untuk mengetahui kekayaan dan keanekaragaman serta menggali informasi mengenai potensi pemanfaatan spesies tanaman pekarangan.
Penelitian ini mencakup keanekaragaman, persepsi dan pengetahuan pemanfaatan spesies tanaman pekarangan pada tiga lokasi altitude (h) yaitu daerah altitude rendah (h≤500 m dpl), altitude menengah (500
Hasil penelitian menunjukkan jumlah spesies yang ditemukan sebanyak 304 spesies dari 229 genus dan termasuk dalam 95 famili. Kekayaan spesies di daerah rendah sebanyak 227 spesies, menengah 202 spesies dan tinggi 156 spesies. Hasil perhitungan indeks keanekaragaman spesies (H?) Shannon-Wiener pada 3 kategori altitude menunjukkan penurunan keanekaragaman seriring dengan peningkatan ketinggian. Hasil analisis dengan Local User's Value Index (LUVI) diperoleh 9 kategori guna dari keseluruhan lokasi penelitian yaitu bahan makanan, hiasan atau ornamen, ritual, peneduh atau perindang, obat-obatan, penulak bala (mitos), sumber penghasilan, menyama braya (sosial) dan pewarna, namun ditemukan perbedaan persepsi fungsi pekarangan bagi masyarakat pada tiap daerah ketinggian. Spesies tanaman dengan nilai kepentingan budaya (ICS-Index of Cultural Significance tertinggi adalah nyuh biasa (Cocos nucifera) sebesar 156 dengan 14 jumlah pemanfaatan.
Balinese homegarden at Pakraman villagers in Bali, is based on the concept of Tri Hita Karana (THK). The concept of managing the yard space to create a harmonious relationship with the creator (Parahyangan), human (Pawongan) and the environment (Palemahan). Ecological research conducted in the village Pakraman homegarden, Buleleng Bali aims to find and explore the richness and diversity of plants spescies and also to get information about the potential use of plants species.
This study includes diversity, perceptions and knowledge utilization homegarden plant species in three locations height (h) that is a low area (h ≤500 m asl), medium (500 < h <1000 m above sea level) and high (h ≥1000 m asl) to further grouped by extents (a) is a small yard (a ≤300 m2), medium (300
The results showed the number of species found as many as 304 species from 229 genera and included in 95 families. Lower species richness in the area as much as 227 species, 202 species of medium height and 156 species. Results of calculation of the index of species diversity (H ') Shannon-Wiener at 3 height categories showed a decline diversity with increased height. Results of the analysis by the Local User's Value Index (LUVI) gained 9 categories in order of overall research sites are foodstuffs, ornaments, ritual, shade, drugs, penulak bala (myth), source of income, menyama braya (social) and dyes, but found differences in the perception of the homegarden functions for society at every altitude. Plant species named nyuh biasa (Cocos nucifera) has highest Index of Cultural Significance (ICS) value of 156 in 14 types of utilization."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2015
T43640
UI - Tesis Membership  Universitas Indonesia Library
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Septia Ekawati
Struktur komunitas pohon di hutan kota Muhammad Sabki, Kota Jambi / Septia Ekawati
"ABSTRAK
Analisis vegetasi tingkat pohon dan belta di Hutan Kota Muhammad Sabki (HKMS), Kota jambi dilakukan pada bulan Januari - Februari 2012. Penelitian dilakukan dengan menggunakan metode transek yang dikombinasikan dengan metode petak. Pengamatan dilakukan dengan 100 petak yang masing-masing berukuran 10 x 10 m, yang tersebar secara sistematis pada beberapa transek sehingga luas total 1 hektare. Hasil inventaris pohon diameter > 10 cm tercatat 49 spesies, dari 489 individu pohon dengan total luas bidang dasar 4,46 m2. Tercatat pula untuk tingkat belta sebanyak 50 spesies dengan total luas bidang dasar adalah 0,15 m . Karet (Hevea brasiliensis Mull. Arg.) masih mendominasi pada tingkat pohon, yang diikuti oleh Litsea firma dengan nilai kepentingan (NK) berturut - turut 74,0% dan 59,3%. Kehadiran pohon karet menunjukkan bahwa hutan kota merupakan hutan sekunder. Analisis sebaran spesies membentuk 11 asosiasi yang terbentuk menjadi beberapa subasosiasi. Hasil analisis keanekaragaman komposisi spesies asli pada zona pemanfaatan sedang (ZPS) lebih tinggi dibandingkan dengan zona pemanfaatan rendah (ZPR) yang merupakan zona inti. Sebanyak 43 spesies (47,7%) pohon dengan dbh > 10 cm adalah tanaman asli yang tumbuh secara alami, dan beberapa di antaranya termasuk dalam katgori langka dan dilindungi oleh hukum di Indonesia.
ABSTRACT
Vegetation analysis of tree and sapling components in the Hutan Kota Muhammad Sabki (HKMSA KotaJambi conducted from January until February 2012. The study used combination of transect and plot method, where 100 plot of 10m x 10m each were spread out systematically along the transect, thus totals I hectare. A total of489 trees (diameter at breast hight, dbh > 10 cm) with total basal area 4,46 m2 comprising 49 species were recorded. The sapling recorded were 50 species consisting of 641 individual, with a total basal area is 0,15 m2. Rubber (Hevea brasiliensis Mull. Arg.) and Litsea flrma are dominants with Importance value 74,0% and 59,3%. The presence of rubber trees indicate that the forest is a secondary forest Analisis of species distribution in trees plot results in 11 species association with several subassositiation. They reveal that zona pemanfaatan sedang (ZPS) diversity the native species composition in ZPS is in higher than in zona pemanfaatan rendah (ZPR). As many as 43 (47,7%) species >10 cm are native plant that grows naturally, some of which are included in the category of rare and protected by the laws of Indonesia.
"
2012
T44104
UI - Tesis Membership  Universitas Indonesia Library
cover
Rizky Priambodo
Karakterisasi kandidat protein alergen serbuk sari kelapa sawit elaeis guineensis jacq serta pengujian aktivitasnya terhadap produksi iga ige igg igm dan ifn gamma pada peripheral blood mononuclear cell pbmc secara in vitro = Characterization of protein allergen candidate from oil palm elaeis guineensis jacq pollen and its activities to iga ige igg igm and ifn gamma production in peripheral blood mononuclear cell pbmc by in vitro
"ABSTRAK
Kelapa sawit merupakan tanaman perkebunan yang banyak dibudidayakan di Indonesia, dengan luas sekitar 11 juta hektar pada tahun 2014. Serbuk sari kelapa sawit memiliki potensi alergi yang cukup besar, karena memiliki ukuran relatif kecil, berjumlah relatif banyak, dan bersifat anemofili.
Penelitian ini bertujuan untuk mengetahui karakter kandidat protein alergen serbuk sari kelapa sawit melalui metode SDS-PAGE dan Western Blotting, serta mengetahui aktivitas IgA, IgE, IgG, IgM, dan IFN-γ pada sel Peripheral Blood Mononuclear Cell (PBMC) terhadap induksi protein serbuk sari kelapa sawit yang dilakukan secara in vitro. Penelitian diawali dengan ekstraksi protein serbuk sari kelapa sawit, yang berasal dari beberapa wilayah di Indonesia. Berat molekul protein dianalisis dengan metode SDS-PAGE, serta uji kealergenikan kandidat protein alergen diuji dengan menggunakan 21 serum pasien alergi melalui metode Western Blotting. Protein serbuk sari kelapa sawit juga diinduksikan pada kultur sel PBMC. Proses pendeteksian IgA, IgE, IgG, IgM, dan IFN-γ dilakukan menggunakan metode ELISA. Berat molekul protein serbuk sari kelapa sawit diketahui berukuran 10?80 kDa.
Hasil uji kealergenikan protein tersebut pada Western Blotting menunjukkan kandidat protein alergen memiliki ukuran 14 kDa, 15 kDa, 20 kDa dan 31 kDa. Aktivitas beberapa immunoglobulin dan sitokin berhasil terdeteksi. Konsentrasi IgA didapatkan sebesar 0,022 pg/ml, IgE sebesar 9,655 pg/ml, IgG sebesar 39,856 pg/ml, IgM sebesar 10,369 pg/ml, dan IFN-γ sebesar 2.617,240 pg/ml.
ABSTRACT
Oil palm is a plant that widely cultivated in Indonesia, with an area of about 11 million hectares in 2014. Oil palm pollen is potential to caused allergy, because it has a small size, much in amount, and was dispersed by wind.
This study aims to determine the character of the allergen protein candidate from oil palm pollen by using SDS-PAGE and Western Blotting, and also to know the activity of IgA, IgE, IgG, IgM, and IFN-γ against exposure to oil palm pollen protein performed in vitro on Peripheral Blood Mononuclear Cell (PBMC). The study begins with the protein extraction from oil palm pollen, which is derived from several regions in Indonesia. The molecular weight of these proteins are analyzed using SDS-PAGE. Allergenic test of allergen protein candidates were tested using 21 serum of allergic patients through Western Blotting method. Oil palm pollen protein also induced in PBMC cultures. The detection of IgA, IgE, IgG, IgM, and IFN-γ were performed using ELISA. The molecular weight of oil palm pollen protein is about 10?80 kDa.
Allergenic test results through Western Blotting showed the allergen protein candidates have a size of 14 kDa, 15 kDa, 20 kDa and 31 kDa. Immunoglobulin and cytokine activity successfully detected. The IgA concentrations obtained 0.022 pg/ml, IgE obtained 9.655 pg/ml, IgG obtained 39.856 pg/ml, IgM obtained 10.369 pg/ml, and IFN-γ obtained 2,617.240 pg /ml."
Depok: Universitas Indonesia, 2015
T44879
UI - Tesis Membership  Universitas Indonesia Library
cover
Azni Ananda
Isolasi, seleksi, identifikasi, dan kemampuan isolat bakteri tanah mendegradasi Propoxur (2-Isopropoksifenil-NMeilkarbamat) = Isolation, selection, identification, and the ability of soil bacterium isolate degrading Propoxur (2- Isopropoxyphenyl-N-Methylcarbamate)
"Propoxur (2-isopropoksifenil-N-metilkarbamat) merupakan insektisida yang berpotensi merusak lingkungan. Kecepatan degradasi propoksur di lingkungan diduga disebabkan peningkatan aktivitas bakteri tanah pendegradasi pestisida. Tujuan penelitian ini adalah untuk memperoleh, mengidentifikasi, dan menguji kemampuan bakteri pendegradasi propoksur. Isolasi dan seleksi dilakukan dengan metode kultur diperkaya. Identifikasi dilakukan dengan analisis filogenetik gen 16S rDNAdibandikan dengan karakter morfologi dan fisiologi. Kemampuan bakteri mendegredasi propoksur diukur pada medium yang mengandung propoksur sebagai sumber karbon pada konsentrasi yang bervariasi. Penurunan konsentrasi propoksur pada medium dianalisis dengan metode spektrofotometri diazotisasi-2-aminopiridina dan KCKT. Pertumbuhan dan kemampuan mendegradasi propoksur juga diukur pada medium dengan pH bervariasi. Aktivitas enzim diukur dengan metode sel istirahat. Enam isolat diperoleh mampu tumbuh dalam propoxur sebagai konsorsium. Satu isolat potensial memiliki kemampuan mendegradasi dan menggunakan propoksur sebagai sumber karbon sebagai kultur tunggal yakni isolat IE. Hasil analisis filogenetik gen 16S rDNA, serta karakter morfologi dan fisiologi menunjukkan isolat IE adalah Rhodococcus pyridinivorans. Bakteri tumbuh dan mendegradasi propoksur menjadi 2-isopropoksifenol dan metilamina dan menggunakan 2- isopropoksifenol sebagai sumber karbon, optimum pada pH 8.
Propoxur (2-isopropoxyphenyl-N-methylcarbamate) was an insecticide that has potential environmental impact. Enhanced degradation propoxur in environment is presumably the result of an increase of activities of soil pesticidedegrading bacteria. This research aims to obtain, to identify, and to test the ability of bacteria degrading propoxur. Isolation and selection was done by enrichment culture method. Identification was done by phylogenetic analysis of 16S rDNA gene compared with morphological and physiological character. The ability of the bacteria to degrade propoxur was measured on medium contain propoxur as sole carbon source in variation concentration. Propoxur in medium was analyzed by diazotized-2-aminopyridine spectrophotometry and HPLC. The ability to growth and to degrade the propoxur was measured on medium with variation of pH. Enzyme activity was measured by resting cell method. Six isolates was obtain growth in propoxur as consortium. One potential isolate has the ability degrading and using propoxur as sole carbon source as a single culture designated as isolate IE. Result of phylogenetic analysis of 16S rDNA gene, morphological and physiological character showed isolate IE is Rhodococcus pyridinivorans. The bacterium grows and degrades propoxur into 2-isopropoxyphenol and methylamine utilized 2-isopropoxyphenol as sole source of carbon, optimum at pH 8."
Depok: Universitas Indonesia, 2012
T44749
UI - Tesis Membership  Universitas Indonesia Library
cover
Eka Gustiana
Variasi morfologi Ahaetulla prasina boie, 1827 squamata: colubridae di Indonesia = Morphological variation of Ahaetulla prasina boie, 1827 squamata: colubridae in Indonesia
"ABSTRAK
Telah dilakukan penelitian mengenai variasi morfologi organ vegetatif
tanaman bidara upas (Merremia mammosa) yang dikumpulkan di daerah jawa
serta aktivitasnya sebagai anti-plasmodium secara in-vitro. Penelitian bertujuan
untuk memperoleh informasi karakter morfologi organ vegetatif tanaman bidara
upas dan aktivitas anti-plasmodium secara in-vitro. Tahapan penelitian meliputi
pengambilan sampel di lapangan, pengamatan morfologi secara visual, ekstraksi,
skrining fitokimia, uji aktivitas antimalaria ssecara in-vitro. Hasil penelitian
menunjukkan sembilan sampel tanaman yang diamati membentuk dua kelompok
utama yaitu kelompok PKL, HAJ dan Purwakarta serta kelompok JJ, HAA,
Balittro, KRP, NL dan KRB. Dua kelompok utama dapat dibedakan berdasarkan
karakter permukaan daun lebih agak kasar (HAJ) atau lebih licin mengkilat
(Purwaka), bentuk umbi, warna pangkal umbi,warna permukaan umbi, banyaknya
serat umbi, warna daging umbi setelah kering, kulit umbi, getah umbi dan warna
akar umbi. Hasil skrining fitokimia kesembilan sampel umbi tanaman bidara upas
(Merremia mammosa) menunjukkan bahwa kesembilan umbi tanaman bidara
upas memiliki kandungan senyawa aktif yang sama yaitu mengandung senyawa
flavonoid, saponin dan terpenoid. Sehingga secara fitokimia, dari kesembilan
sampel esktrak n-heksan umbi bidara upas, diambil satu sampel yaitu sampel
ekstrak n-heksan dari Juragan Jamu (JJ) dari Sleman Jogyakartau ntuk diuji
aktivitas anti-plasmodium. Hasil uji aktivitas anti-plasmodium menunjukkan
bahwa ekstrak n-heksan umbi bidara upas bersifat anti-plasmodium dengan nilai
IC50 3,36, sehingga umbi bidara upas memiliki aktivitas kuat sebagai antiplasmodium
secara in-vitro
ABSTRACT
Morphological Variation study on plant vegetative organs of bidara upas
(Merremia mammosa) collected in the area of Java and its activities antiplasmodium
as in-vitro. The aim of the study is to obtaining information on
morphological characters of vegetative organs of plants bidara upas collected in
the area Java and anti-plasmodium activity in vitro. The study include field
sampling, visual morphological observation, extraction, phytochemical screening,
and testing antimalarial activity in-vitro. The results showed whole plant samples
were observed to form two main groups, namely the first group of PKL, HAJ and
Purwakarta and a second group consisting of JJ, HAA, Balittro, KRP, NL and
KRB. The two main groups can be distinguished by the character form bulbs,
tubers base color, the color of the surface of the bulb, the amount fiber of bulb,
such as tuber flesh color after drying, tubers, bulbs and color sap tuber. The results
of nine samples of phytochemical screening tubers of plants bidara upas
(Merremia mammosa) showed that all nine plant bulbs bidara upas contains
flavonoids, saponins and terpenoids. So that phytochemicals, of the nine samples
of n-hexane extract the tubers bidara upas, was taken one sample of n-hexane
extracts of Juragan Jamu (JJ) from Yogyakarta's Sleman was tested antiplasmodium
activity. Anti-plasmodium activity test results showed that n-hexane
extract the tubers are bidara upas anti-plasmodium with IC50 values of 3.36, so the
bulbs bidara upas have strong activity as anti-plasmodium in vitro"
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2016
T46771
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Salaki, Larissa Deviani
Komunikasi vokal pada kelasi presbytis rubicunda di hutan Sabangau, Kalimantan Tengah = Vocal communication of maroon langurs presbytis rubicunda in sabangau forest Central Kalimantan / Larissa Deviani Salaki
"ABSTRAK
Studi ini menyediakan analisis awal mengenai komunikasi vokal pada kelasi
(Presbytis rubicunda). Bagian pertama dari studi ini mengidentifikasi dan
mendeskripsikan repertoar vokal yang dimiliki oleh kelasi beserta konteks
penggunaan dan struktur akustiknya. Bagian kedua menganalisis fungsi dari salah
satu jenis vokalisasi pada kelasi, yaitu loud call, dengan mengidentifikasi variasi
akustik pada loud call antarkonteks dan antarindividu. Sebanyak 574 rekaman
vokal dari 3 kelompok kelasi di Laboratorium Alam Hutan Gambut, Sabangau,
Kalimantan Tengah digunakan dalam analisis. Analisis dilakukan secara akustik
dan secara statistik menggunakan statistik deskriptif dan fungsi diskriminan.
Hasil analisis menunjukkan bahwa repertoar vokal kelasi terdiri dari setidaknya
10 jenis vokalisasi yang dapat dibedakan berdasarkan struktur akustiknya (p <
0,05). Kelasi betina dewasa memiliki jenis vokalisasi terbanyak (5 jenis), diikuti
oleh bayi (4 jenis), dan jantan dewasa (2 jenis). Kelasi menggunakan repertoar
vokal tersebut dalam berbagai macam konteks yang digunakan untuk
berkomunikasi ke luar kelompok (saat bertemu kelompok kelasi lain, melihat
predator atau manusia) atau dengan sesama anggota kelompok (berpindah, makan,
dan interaksi antara ibu dan bayi). Hasil analisis fungsi diskriminan juga
menunjukkan bahwa loud call kelasi memiliki variasi akustik yang signifikan (p <
0,05) antarindividu maupun antarkonteks yang memungkinkan pendengarnya
untuk mengetahui identitas kelasi pemanggil dan konteks dari panggilan tersebut
ABSTRACT
This study provides a preliminary analysis on maroon langurs? (Presbytis
rubicunda) vocal communication. The first section of this study identified maroon
langurs? vocal repertoire as well as its associated contexts and acoustic structure.
This second section analyzed the function of maroon langurs? loud call by
identifying variations in its acoustic feature between contexts and individuals. A
total of 574 recordings from 3 maroon langur groups in Sabangau Natural
Laboratory of Peat Swamp Forest, Central Kalimantan were used in this study.
Analyses were performed acoustically and statistically using descriptive statistics
and discriminant function analysis. Our analyses showed that maroon langurs?
vocal repertoire consisted of at least 10 types of vocalization that can be
discriminated by its acoustic structure (p < 0.05). Adult females had the largest
repertoire (5 types), followed by infants (4 types), and adult males (2 types).
Maroon langurs used vocalizations in various contexts to facilitate extragroup
communication (such as group encounter, presence of predator or human) or
intragroup communication (traveling, feeding, and mother-infant interaction).
Discriminant function analysis also showed that maroon langurs? loud calls
possessed significant acoustic variation (p < 0.05) between individuals and
contexts, which may inform the receivers about the identity of the caller and the
context of the call."
2016
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Eka Sari Nurhidayati
Kontribusi mangrove terhadap produksi unsur hara dan cadangan karbon di Tanjung Lesung, Banten = Mangrove contributions on nutrient production and carbon stock in Tanjung Lesung, Banten
"Penelitian di ekosistem mangrove Tanjung Lesung, Banten bertujuan untuk mendapatkan informasi tentang struktur dan komposisi vegetasi; potensi produksi dan kecepatan dekomposisi serasah, dan produksi C,N, P; serta kemampuan menyimpan dan menyerap karbon mangrove. Struktur dan komposisi vegetasi diukur dengan transek kuadrat dengan total luasan pengamatan 3300 m2. Produksi serasah dihitung menggunakan perangkap serasah ukuran 1x1 m2. Laju dekomposisi serasah diukur selama 84 hari dengan pengamatan setiap 14 hari sekali. Cadangan karbon diestimasi dengan persamaan allometrik. Total spesies vegetasi yang ditemukan di areal penelitian adalah 7 spesies dari 6 famili. Vegetasi tingkat pohon dan belta didominasi oleh Lumnitzera racemosa dengan kerapatan 670 pohon/ha dan 2252 pohon/ha. Produksi serasah sebesar 1,571 ± 0,924 g/m2/hari, tersusun atas daun 1,563 ± 0,916 gr/m2/hari (99,50%) dan ranting sebesar 0,008 ± 0,048 gr/m2/hari (0,50%). Laju dekomposisi serasah sebesar 0,09 ± 0,07 gr/hari dengan persentase serasah daun yang terdekomposisi/hilang sebesar 47,9 ± 15,5%. Potensi unsur hara dari serasah daun sebesar 0,025 ± 0,002 g C/m2/hari; dan 0,001 ± 0,0006 g N/m2/hari; serta 0,0003 ± 0,00026 g P/m2/hari. Rata-rata unsur karbon yang terlepas dari serasah daun selama proses dekomposisi sebesar 5,36 ± 2,24%, sementara untuk nitrogen sebesar 0,009 ± 0,008%, dan total phosfat sebesar 0,0012 ± 0,00038%. Biomassa dan kandungan karbon di atas dan bawah permukaan tanah sebesar 24,29 ton/ha dengan 11,4 ton C/ha, kandungan karbon tanah sebesar 127,88 ton C/ha. Total cadangan karbon mangrove di Tanjung Lesung sebesar 139,296 ton C/ha, sebesar 91,8% cadangan karbon tersimpan dalam tanah. Kemampuan menyerap CO2 atmosfer sebesar 24,522 Ton CO2/ha untuk tingkat pohon dan 4,79 Ton CO2/ha untuk tingkat anakan.
Research in mangrove ecosystem of Tanjung Lesung, Banten aims to obtain information of vegetation structure and composition; production, decomposition rates, nutrient contribution of mangrove litter; and potential carbon stocks. Structure and composition of vegetation measured by quadrant method, with total observation area is 3300 m2. Litter production was collected using the litter-trap (1 x 1m) during two months. Litter decomposition rates were measured for 84 days with observations every 14 days. Carbon stock are estimated by allometric equation. The diversity of mangrove vegetation consists of 7 species from 6 families. At the tree level and sapling, vegetation is dominated by Lumnitzera racemosa has the density around 670 tree/ha and 2252 tree/ha. Litter production is about 1,571 ± 0,924 g/m2/day, that consist of leaf 1,563 ± 0,916 gr/m2/day (99,50%) and stalk 0,008 ± 0,048 gr/m2/day (0,50%). Litter decomposition rate is about 0,09 ± 0,07 gr/day with the percentage of litter decomposed of 47,9 ± 15,5%. The potential of litter nutrient are 0,025 ± 0,02 g C/m2/day; 0,001 ± 0,0006 g N/m2/day; and 0,0003 ± 0,00026 g P/m2/day. Carbon average that was detached from litter during decomposition is 5,36 ± 2,24%, while for nitrogen is 0,009 ± 0,008%, and total phosphate is 0,0012 ± 0,00038%. Biomass and carbon stock above and below the ground surface are 24,29 ton/ha with 11,4 tons C/ha. Carbon stock of sedimen mangrove is 127,88 ton C/ha. Total carbon stock of mangrove in Tanjung Lesung, Banten is about 139,296 ton C/ha, where 91,8% of them stored in sediment mangrove. The ability to absorb CO2 in atmosphere is 24,522 tons CO2/ha for trees level and 4,79 tons CO2/ha for sapling."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2016
T46072
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Eka Gustiana
Variasi morfologi organ vegetatif bidara upas (merremia mammosa (lour.) hallier f) yang dikumpulkan di daerah Jawa serta aktivitasnya sebagai anti-plasmodium secara in-vitro = Variation in bidara upas's vegetative organ morphology in Java and its potential as in-vitro anti- plasmodiums / Eka Gustiana
"ABSTRAK
Telah dilakukan penelitian mengenai variasi morfologi organ vegetatif
tanaman bidara upas (Merremia mammosa) yang dikumpulkan di daerah jawa
serta aktivitasnya sebagai anti-plasmodium secara in-vitro. Penelitian bertujuan
untuk memperoleh informasi karakter morfologi organ vegetatif tanaman bidara
upas dan aktivitas anti-plasmodium secara in-vitro. Tahapan penelitian meliputi
pengambilan sampel di lapangan, pengamatan morfologi secara visual, ekstraksi,
skrining fitokimia, uji aktivitas antimalaria ssecara in-vitro. Hasil penelitian
menunjukkan sembilan sampel tanaman yang diamati membentuk dua kelompok
utama yaitu kelompok PKL, HAJ dan Purwakarta serta kelompok JJ, HAA,
Balittro, KRP, NL dan KRB. Dua kelompok utama dapat dibedakan berdasarkan
karakter permukaan daun lebih agak kasar (HAJ) atau lebih licin mengkilat
(Purwaka), bentuk umbi, warna pangkal umbi,warna permukaan umbi, banyaknya
serat umbi, warna daging umbi setelah kering, kulit umbi, getah umbi dan warna
akar umbi. Hasil skrining fitokimia kesembilan sampel umbi tanaman bidara upas
(Merremia mammosa) menunjukkan bahwa kesembilan umbi tanaman bidara
upas memiliki kandungan senyawa aktif yang sama yaitu mengandung senyawa
flavonoid, saponin dan terpenoid. Sehingga secara fitokimia, dari kesembilan
sampel esktrak n-heksan umbi bidara upas, diambil satu sampel yaitu sampel
ekstrak n-heksan dari Juragan Jamu (JJ) dari Sleman Jogyakartau ntuk diuji
aktivitas anti-plasmodium. Hasil uji aktivitas anti-plasmodium menunjukkan
bahwa ekstrak n-heksan umbi bidara upas bersifat anti-plasmodium dengan nilai
IC50 3,36, sehingga umbi bidara upas memiliki aktivitas kuat sebagai antiplasmodium
secara in-vitro.
ABSTRACT
Morphological Variation study on plant vegetative organs of bidara upas
(Merremia mammosa) collected in the area of Java and its activities antiplasmodium
as in-vitro. The aim of the study is to obtaining information on
morphological characters of vegetative organs of plants bidara upas collected in
the area Java and anti-plasmodium activity in vitro. The study include field
sampling, visual morphological observation, extraction, phytochemical screening,
and testing antimalarial activity in-vitro. The results showed whole plant samples
were observed to form two main groups, namely the first group of PKL, HAJ and
Purwakarta and a second group consisting of JJ, HAA, Balittro, KRP, NL and
KRB. The two main groups can be distinguished by the character form bulbs,
tubers base color, the color of the surface of the bulb, the amount fiber of bulb,
such as tuber flesh color after drying, tubers, bulbs and color sap tuber. The results
of nine samples of phytochemical screening tubers of plants bidara upas
(Merremia mammosa) showed that all nine plant bulbs bidara upas contains
flavonoids, saponins and terpenoids. So that phytochemicals, of the nine samples
of n-hexane extract the tubers bidara upas, was taken one sample of n-hexane
extracts of Juragan Jamu (JJ) from Yogyakarta's Sleman was tested antiplasmodium
activity. Anti-plasmodium activity test results showed that n-hexane
extract the tubers are bidara upas anti-plasmodium with IC50 values of 3.36, so the
bulbs bidara upas have strong activity as anti-plasmodium in vitro"
2016
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UI - Tesis Membership  Universitas Indonesia Library
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Ristiyanti Marsetiyowati Marwoto
Keong air tawar suku Thiaridae di Danau Poso studi Morfologi, anatomi marga Tylomelania dari danau Poso, Sulawesi Tengah (Moluska : Gastropoda Caenogastropoda)
"There are 49 nominal taxa of Thiaridae known from Sulawesi and 11 species from Lake Poso, Central Sulawesi have been reported by Sarasin & Sarasin (1897 & 1898). Since then, no study on the systematic of the species from Lake Poso have been carried out. The basic information on the morphology of those species were described for the first time in 1897 & 1898. However, information on their habitat and distribution are still lacking.
Lake Poso is a tectonic-lake, which lies at 510 m above sea level and covers about 32,000 hectares. The deepest part of the lake reaches 450 meters. To study the diversity and distribution of Thiaridae in Lake Poso, snails were collected from 33 locations (outlet, inlet, and area of the lake), using sieve and by hand (at the shallow area) or small hand ?dredge? (at deep area). Morphology characters (height, width of shell and aperture; number of axial and spiral ribs at the penultimate and body whorls) were examined. Anatomical study (head, neck, tentacle, eye, snout, sole, foot groove); pallial cavity (ctenidium, osphradium, rectum, mantle edge); alimentary system (buccal mass, radula sac, radula, stomach); reproductive system (female: brood pouch, bursa copulatrix, albumen gland, egg, embryo, ovary; male: prostate gland, testis, testicle lobes, vas deferens); nervous system (cerebro ganglion, pleuro ganglion, pedal ganglion) was conducted only on Tylomelania.
Totally there are 13 species and more than eight undescribed species of Thiaridae identified in this study. Most of the species belong to the genus Brofia which occurred at the area of the lake (LP 10, LP 11, LP 12, LP 13, LP 15, LP 16, LP 17, LP 17 a, LP 18, LP 19, LP 20, LP 21, LP 22, LP 23, LP 24, LP 24 a, LP 25, LP 26, LP 27, LP 28, LP 29, LP 31), outlet (LP 1, LP 2, LP 3, LP 4) and inlet area (LP 5, LP 6, LP 7, LP 8, LP 9, LP 30). B. toradjarum, B. sca/ario psis, B. ku/i, B. centaurus were found at the area of the lake, while B. perfecta and B. robusta at the inlet and outlet respectively. There are two species of Melanoides (M. tuberculata & M. granífera) found at the area of the lake, and one species, M. punctata, occurred only at the stream of Saluopa (inlet area).
The anatomy of the genus Tylomelania was described for the first time. Comparative study on the morphology and anatomy were based on specimen of T. neritiformis, T. porcellanica, and T. carbo. The anatomical characters described and compared: the length and width of buccal mass, radula sac, ctenidium, osphradium, stomach, style-sac, albumen gland, eggs, and embryos, cerebral commissure, pedal commissure. The shape and position of anus, rectum, ctenidium, osphradium, radula, brood pouch, bursa copulatrix, albumen gland, genital Opening, prostate gland, ovary, testis, cerebro ganglion, pleuro ganglion, pedal ganglion, suboesophageal ganglion, supraoesophageal ganglion presented for the first time."
2000
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