Hasil Pencarian  ::  Simpan CSV :: Kembali

"Bacterial lipopolysaccharide (LPS) is impacted in the etiology of inflammatory periodontal disease. Aside from immunopathologic reactions which may be involved in the pathogenesis of the disease, the possibility exist that direct cytotoxic effect on cultured human gingival fibroblasts may be equally destructive. The expression of P53 protein can be one of markers to examine the state of impaired DNA. The purpose of this study was to investigate the effect of LPS toward expression of P53 protein on cultured human gingival fibroblasts. Cultured human gingival fibroblasts were exposed to LPS in concentrations of 50 and 200 ug/ml and untreated medium for a period of 24 and 48 hours. Cells were harvested and prepared for immunohistochemical evaluation. After exposure for 24 and 48 hours, the fraction of P53-positive cells was 81.7% in case of 50 ug/ml LPS, and 88.8% in case of 200 ug/ml LPS. After exposure for 48 hours, the fraction of P53-positive cells was 32.2% in case of 50 ug/ml LPS, and 21.1% in case of 200 ug/ml LPS. None of untreated group showed p53-positive cells. Up-regulation of p53 protein during the initial logarithmic phase of growth may be a consequence of on-going DNA damage."

"Analisis histomorfometrik pembentukan lesi aterosklerosis koroner pada model tikus (Rattus novergicus) periodontitis. Penyakit kardiovaskuler perupakan penyakit paling mematikan di dunia dan penyebab utamanya adalah aterosklerosis. Paradigma baru menjelaskan bahwa inflamasi kronis seperti periodontitis dipertimbangkan sebagai faktor risiko terjadinya aterosklerosis. Periodontitis menyebabkan bakteremia yang berpengaruh terhadap inflamasi vaskuler. Keadaan ini mempengaruhi sel endotel dan oksidasi lipid sehingga meningkatkan resiko aterosklerosis. Tujuan: Penelitian ini bertujuan untuk membuktikan pengaruh periodontitis terhadap pembentukan lesi aterosklerosis koroner. Metode: Penelitian ini merupakan eksperimen menggunakan the post test only control group design. 12 sampel tikus dibagi atas kelompok kontrol (K) tanpa perlakuan dan kelompok periodontitis (P) dibuat dengan pemasangan wire ligature dan injeksi Porphyromonas gingivalis pada M1 rahang bawah kiri selama 28 hari. Pada hari ke-29 tikus didekaputasi, arteri koroner diambil dan dibuat preparat histologi. Analisis histomorfomerik dilakukan pada morfologi dungkul (deskriptif) dan ketebalan dinding arteri (statistik dengan uji T test). Hasil: Hasil penelitian menunjukkan bahwa terbentuknya dungkul arteri lebih banyak pada kelompok Periodontitis (83,33%) daripada kelompok Kontrol (16,67%). Dinding arteri kelompok periodontitis juga lebih tebal (2,22±0,35µm) secara signifikan dibandingkan kelompok kontrol (1,28±0,20µm) (p<0,05). Simpulan: Periodontitis secara independen dapat mempengaruhi gambaran histomorfometrik pembentukan lesi aterosklerosis koroner.

---

Cardiovascular disease is the most deadly disease in the world and the main cause is atherosclerosis. New paradigm explains that chronic inflammation such as periodontitis is considered to be a risk factor for atherosclerosis. Periodontitis causes bacteremia affecting vascular inflammation. This situation affects the endothelial cells and lipid oxidation thereby increasing the risk of atherosclerosis. This study aimed to prove the effect of periodontitis on coronary atherosclerotic lesion formation. Methods: This was an experimental study using the post-test only control group design. Samples were 12 rats divided into control group (K) without treatment and periodontitis (P) was made with the installation of wire ligature and injection of Porphyromonas gingivalis on the left mandibular M1 for 28 days. On day 29, mice were decaputated, coronary artery was taken and histological slides were made. Histomorphometrical analysis was calculated on dungkul (descriptive) and the thickness of the arterial wall (with test statistic T test). Results: The results showed higher formation of arterial dungkul on Periodontitis group (83.33%) than the control group (16.67%). Arterial wall was also thicker periodontitis group (2.22±0.35µm) (p<0.05) than the control group (1.28±0.20µm). Conclusion: Periodontitis can independently affect the coronary atherosclerotic lesion formation."

"Deteksi infeksi HIV-1 dengan teknologi microfluidic chip-based nucleic acid menggunakan cairan krevikular gingiva. Tingginya resiko transmisi infeksi oleh individu dengan infeksi HIV-1 yang masih dalam periode jendela dan tidak terdiagnosis positif HIV-1 oleh alat deteksi yang lain, akan menjadi masalah serius yang timbul dalam masyarakat. Beberapa metode diagnosis yang ada saat ini memiliki beberapa kekurangan seperti dibutuhkannya peralatan laboratorium khusus, antibodi terhadap HIV-1, waktu yang spesifik untuk pemeriksaan, memerlukan sampel pemeriksaan yang diambil secara invasif, waktu pemeriksaan yang lama sampai kurang dapatnya metode ini diakses oleh daerah-daerah yang lokasinya sulit. Sudah banyak dilakukan upaya untuk memecahkan masalah ini untuk mendapatkan suatu teknik deteksi baru. Studi pustaka ini bertujuan untuk memberikan informasi tentang perkembangan teknologi deteksi infeksi HIV-1 yang menggunakan microfluidic chip-based nucleic acid testing (mChip NAT). Studi pustaka ini juga membahas tentang kemungkinan penggunaan cairan krevikular gingival (GCF) sebagai sampel pemeriksaan yang dapat diambil secara takinvasif.

---

Transmission of HIV-1 infection by individuals in window period who are tested negative in conventional HIV-1 detection would pose the community with serious problems. Several diagnostic tools require specific laboratory equipment, perfect timing of diagnosis, antibody to HIV-1, and invasive technique to get sample for examination, until high amount of time to process the sample as well as accessibility of remote areas. Many attempts have been made to solve those problems to come to a new detection technique. This review aims to give information about the current development technique for detection of HIV infection. Microfluidic Chipbased Nucleic Acid Testing is currently introduced for detection of HIV-1 infection. This review also cover the possible usage of gingival crevicular fluid as sample specimen that could be taken noninvasively from the individual."

"Dental caries and periodontal disease are well established and common in Indonesia, and both of these diseases are major concerns for the oral and dental health. The objective of this study was to compare the differences in children with and without caries in terms of colony bacteria in the saliva, and the changes before and after consumption of carbonated drink. The study was conducted on Al-Qodiri elementary school students who were 10-12 years old. Saliva was collected before and after consumption (for 2 min) of a carbonated drink. T-test was used to analyze the results. The results of the study showed that there was a significant difference (p<0.05) between the caries and non-caries groups."