Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
ABSTRAK Kelapa sawit merupakan tanaman perkebunan yang banyak dibudidayakan di Indonesia, dengan luas sekitar 11 juta hektar pada tahun 2014. Serbuk sari kelapa sawit memiliki potensi alergi yang cukup besar, karena memiliki ukuran relatif kecil, berjumlah relatif banyak, dan bersifat anemofili. Penelitian ini bertujuan untuk mengetahui karakter kandidat protein alergen serbuk sari kelapa sawit melalui metode SDS-PAGE dan Western Blotting, serta mengetahui aktivitas IgA, IgE, IgG, IgM, dan IFN-γ pada sel Peripheral Blood Mononuclear Cell (PBMC) terhadap induksi protein serbuk sari kelapa sawit yang dilakukan secara in vitro. Penelitian diawali dengan ekstraksi protein serbuk sari kelapa sawit, yang berasal dari beberapa wilayah di Indonesia. Berat molekul protein dianalisis dengan metode SDS-PAGE, serta uji kealergenikan kandidat protein alergen diuji dengan menggunakan 21 serum pasien alergi melalui metode Western Blotting. Protein serbuk sari kelapa sawit juga diinduksikan pada kultur sel PBMC. Proses pendeteksian IgA, IgE, IgG, IgM, dan IFN-γ dilakukan menggunakan metode ELISA. Berat molekul protein serbuk sari kelapa sawit diketahui berukuran 10?80 kDa. Hasil uji kealergenikan protein tersebut pada Western Blotting menunjukkan kandidat protein alergen memiliki ukuran 14 kDa, 15 kDa, 20 kDa dan 31 kDa. Aktivitas beberapa immunoglobulin dan sitokin berhasil terdeteksi. Konsentrasi IgA didapatkan sebesar 0,022 pg/ml, IgE sebesar 9,655 pg/ml, IgG sebesar 39,856 pg/ml, IgM sebesar 10,369 pg/ml, dan IFN-γ sebesar 2.617,240 pg/ml.

---

ABSTRACT Oil palm is a plant that widely cultivated in Indonesia, with an area of about 11 million hectares in 2014. Oil palm pollen is potential to caused allergy, because it has a small size, much in amount, and was dispersed by wind. This study aims to determine the character of the allergen protein candidate from oil palm pollen by using SDS-PAGE and Western Blotting, and also to know the activity of IgA, IgE, IgG, IgM, and IFN-γ against exposure to oil palm pollen protein performed in vitro on Peripheral Blood Mononuclear Cell (PBMC). The study begins with the protein extraction from oil palm pollen, which is derived from several regions in Indonesia. The molecular weight of these proteins are analyzed using SDS-PAGE. Allergenic test of allergen protein candidates were tested using 21 serum of allergic patients through Western Blotting method. Oil palm pollen protein also induced in PBMC cultures. The detection of IgA, IgE, IgG, IgM, and IFN-γ were performed using ELISA. The molecular weight of oil palm pollen protein is about 10?80 kDa. Allergenic test results through Western Blotting showed the allergen protein candidates have a size of 14 kDa, 15 kDa, 20 kDa and 31 kDa. Immunoglobulin and cytokine activity successfully detected. The IgA concentrations obtained 0.022 pg/ml, IgE obtained 9.655 pg/ml, IgG obtained 39.856 pg/ml, IgM obtained 10.369 pg/ml, and IFN-γ obtained 2,617.240 pg /ml.

Abstrak :
Perkembangan‌ ‌jumlah‌ ‌masyarakat/penduduk‌ ‌yang‌ ‌tidak‌ ‌diikuti‌ ‌dengan‌ ‌perkembangan‌ ‌fasilitas‌ ‌serta‌ ‌utilitas‌ ‌yang‌ ‌memadai‌ ‌tentu‌ ‌akan‌ ‌mengakibatkan‌ ‌defisit‌ ‌dalam‌ ‌pemenuhan‌ ‌kebutuhan‌ ‌masyarakat‌ ‌tersebut.‌ ‌Terdapat‌ ‌isu‌ ‌terkait‌ ‌ terbatasnya‌ ‌penawaran‌ ‌(supply)‌ ‌air‌ ‌yang‌ ‌ada‌ ‌di‌ ‌wilayah‌ ‌DKI‌ ‌Jakarta‌ ‌yang‌ ‌disebabkan‌ ‌oleh‌ ‌bertambahnya‌ ‌jumlah‌ ‌penduduk,‌ ‌terbatasnya‌ ‌supply‌ ‌air‌ ‌baku,‌ ‌tingginya‌ ‌tingkat‌ ‌pencemaran‌ ‌sebagai‌ ‌sumber‌ ‌air‌ ‌permukaan,‌ ‌eksploitasi‌ ‌air‌ ‌secara‌ ‌besar-besaran,‌ ‌dan‌ ‌berkurangnya‌ ‌daerah‌ ‌tangkapan‌ ‌air‌ ‌akibat‌ ‌menurunnya‌ ‌jumlah‌ ‌wilayah‌ ‌serapan.‌ ‌Tujuan‌ ‌ untuk‌ ‌menganalisa‌ ‌bagaimana‌ ‌kualitas‌ ‌air‌ ‌minum‌ ‌yang‌ ‌ada‌ ‌di‌ ‌Jabodetabek‌ ‌berdasarkan‌ ‌data‌ ‌sekunder‌ ‌Susenas‌ ‌(Survei‌ ‌ Sosial‌ ‌Ekonomi‌ ‌Nasional)‌ ‌dan‌ ‌keterkaitannya‌ ‌dengan‌ ‌kondisi‌ ‌sosial,‌ ‌ekonomi,‌ ‌dan‌ ‌demografi‌ ‌rumah‌ ‌tangga‌ ‌dengan‌ ‌ menggunakan‌ ‌metode‌ ‌penelitian‌ ‌kuantitatif‌ ‌analisis‌ ‌menggunakan‌ ‌regresi logistik binary. Hasil menunjukkan pada Provinsi DKI Jakarta sudah memiliki akses sumber air minum layak 90.62% dan Bodetabek sebesar 80.03%. Namun masih terdapat 9.38% rumah tangga di Provinsi DKI Jakarta dan 19.97% wilayah Bodetabek rumah tangga yang tidak mendapatkan akses tersebut. Analisis regresi pada Provinsi DKI Jakarta menunjukkan faktor seperti status migrasi, pendidikan kepala rumah tangga, pengeluaran kapita, daerah lokasi rumah tinggal, keluhan terhadap penyakit, dan kepemilikan asuransi berpengaruh signifikan terhadap ketersediaan air minum layak. Sedangkan pada wilayah Bogor, Depok, Tangerang, dan Bekasi menunjukkan faktor jumlah ART, status menikah, jenis kelamin KRT, pendidikan terakhir, pengeluaran kapita, status kepemilikan rumah, dan daerah rumah tangga berpengaruh signifikan terhadap ketersediaan fisik air minum yang layak. ......The development of the number of communities / residents who are not followed by the development of facilities and adequate utilities will certainly result in a deficit in the fulfillment of the needs of the community. There are issues related to the limited supply (supply) of water in the DKI Jakarta area caused by increasing population , The limited supply of raw water, the high level of pollution as a surface source, massive water exploitation, and reduced water catchment areas due to the decline in the amount of absorption area. The purpose of analyzing how the quality of drinking water in Jabodetabek is based on Secondary Susenas data (National Socio-Economic Survey) and its relevance to household social, economic, and demographic conditions using the research method AND quantitative analysis using binary logistic regression. The results showed that in DKI Jakarta Province already had access to drinking water sources worth 90.62% and Bodetabek was 80.03%. But there are still 9.38% of households in DKI Jakarta Province and 19.97% of household bodetabek areas that do not get these access. Regression analysis in DKI Jakarta Province showed factors such as migration status, household head education, capita expenditure, residential location area, complaints against disease, and insurance ownership significantly influence the availability of decent drinking water. Whereas the Bogor, Depok, Tangerang and Bekasi regions show factor number of ART, married status, KRT gender, last education, capita expenditure, home ownership status, and household regions have a significant effect on the physical availability of decent drinking water.

Abstrak :
Transferin merupakan salah satu faktor yang berperan penting dalam transportasi zat besi dalam darah dan dapat mampu menyebabkan ikan nila (Oreochromis niloticus) hidup pada rentang salinitas yang cukup besar. Penelitian bertujuan merekonstruksi primer PCR spesifik untuk gen transferin pada ikan nila (Oreochromis niloticus). Gen transferin yang diuji berasal dari 10 strain ikan nila yang mewakili populasi ikan nila di Indonesia. Hasil PCR gen transferin ikan nila strain GESIT, Red Nifi, dan Selfam kemudian diklona dan disekuensing. Sekuen DNA yang didapatkan dihomologikan dengan sekuen gen transferin yang terdapat pada gene bank. Hasil homologi menunjukkan adanya daerah yang conserved sebesar 83 pb. Primer didesain untuk mengamplifikasi fragmen gen transferin tersebut. Hasil penelitian menunjukkan bahwa primer TrfNF dan TrfNR mampu mengamplifikasi gen transferin dari 10 strain ikan nila. ......Transferrin is one of many factors that makes Nile Tilapia fish (Oreochromis niloticus) can live in very wide salinity range. The purpose of this research is to reconstruct specific primer for transferrin gene in Nile tilapia fish (Oreochromis niloticus). The transferrin gene was taken from 10 variant of Nile Tilapia fish which represent the population of Nile Tilapia fish in Indonesia. The result from amplification process of transferrin gene from Nile Tilapia variant GESIT, Red Nifi, and Selfam was cloned and was sequenced. The result of sequencing process was arranged to find the conserved region. The conserved region has found and the size is 83 bp. PCR primer was designed to amplify transferrin gene fragmen. The result of this research is TrfNF primer and TrfNR primer can amplify transferrin gene from 10 variant Nile Tilapia fish.