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Penelitian ini bertujuan untuk menguji kemampuan Rhizopus azygosporus UICC 539 dalam mendegradasi pati 1% dan 2% pada suhu 30ºC, 35ºC, 40ºC, 45ºC, dan 50º C. Rhizopus azygosporus UICC 539 di Potato Sucrose Agar (PSA) usia 5 hari pada suhu 30ºC dibuat menjadi blok agar berdiameter 6 mm. Blok agar mengandung sel R. azygosporus (106 sel/mL) ditanam pada Czapek Dox Agar (CDA) modifikasi dengan penambahan pati 1% (b/v) dan 2% (b/v), kemudian diinkubasi pada suhu 30ºC, 35ºC, 40ºC, 45ºC, dan 50º C, selama 3 dan 5 hari. Kontrol adalah CDA modifikasi dengan pati 1% dan 2% tanpa blok agar serta CDA tanpa pati sebagai kontrol negatif. Hasil degradasi pati ditunjukkan sebagai zona bening dengan menambahkan larutan Lugol iodin pada medium perlakuan setelah 3 dan 5 hari. Kemampuan degradasi pati dihitung menggunakan Enzymatic Index (EI) dengan R/r, yaitu R adalah diameter zona bening dan r adalah diameter koloni. Hasil penelitian menunjukkan bahwa R. azygosporus UICC 539 mampu mendegradasi pati 1% dan 2% pada suhu 30º–50ºC. Kemampuan R. azygosporus UICC 539 mendegradasi pati semakin meningkat, seiring peningkatan suhu pertumbuhan dan waktu inkubasi. ......This study aims to test the ability of Rhizopus azygosporus UICC 539 to degrade 1% and 2% starch at temperatures of 30ºC, 35ºC, 40ºC, 45ºC, and 50º C. Five-day old R. azygosporus UICC 539 in Potato Sucrose Agar (PSA) at 30ºC was made into agar blocks in 6 mm diameter. Agar blocks containing R. azygosporus cells (106 cells/mL) were grown on modified Czapek Dox Agar (CDA) with the addition of 1% (w/v) and 2% (w/v) starch, and incubated at 30ºC, 35ºC, 40ºC, 45ºC, and 50ºC, for 3 and 5 days. Controls were modified CDA with 1% (w/v) and 2% (w/v) starch without agar blocks and CDA without starch as negative control. Indication of starch degradation was shown as a clear zone by adding Lugol’s iodine solution to the medium after 3 and 5 days. The ability of R. azygosporus UICC 539 to degrade starch was calculated using Enzymatic Index (EI) formulation: R/r, where R was the diameter of the clear zone and r was the diameter of the colony. The results showed that R. azygosporus UICC 539 was able to degrade 1% and 2% starch at 30ºC–50ºC. The ability of R. azygosporus UICC 539 to degrade starch increased with increasing temperature and incubation time.