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"ABSTRAKTelah dilakukan penelitian mengenai pemanfaatan teknik adaptasi danimobilisasi sel khamir Candida tropicalis InaCC Y799 pada fermentasi xilitol darihidrolisat hemiselulosa daun tebu (Saccharum officinarum L.). Praperlakuan dauntebu dilakukan menggunakan 1,8% asam maleat dan iradiasi gelombang mikropada suhu 180 C selama 5 menit. Penelitian bertujuan untuk mempelajarikemampuan khamir untuk tumbuh dan beradaptasi pada hidrolisat hemiselulosasebelum fermentasi, dan meneliti potensi penggunaan matriks kalsium alginatuntuk imobilisasi sel khamir, serta pengaruhnya dalam peningkatan produksixilitol dari hidrolisat hemiselulosa daun tebu. Hasil menunjukkan bahwa khamirC. tropicalis InaCC Y799 teradaptasi mampu tumbuh pada media hidrolisathemiselulosa daun tebu. Adaptasi khamir pada 75% hidrolisat menghasilkankonsentrasi dan rendemen xilitol maksimum masing-masing sebesar 11,27 1,65g/L dan 0,56 0,05 g/g (54,98% dari nilai rendemen teoritis) selama 24 jamfermentasi, lebih tinggi daripada khamir tidak teradaptasi (kontrol). Namundemikian, imobilisasi C. tropicalis pada kalsium alginat hanya menghasilkankonsentrasi dan rendemen xilitol maksimum masing-masing sebesar 5,51 0,63g/L dan 0,27 0,04 g xilitol/g xilosa awal (29,97% dari rendemen teroritis)selama 48 jam fermentasi. Konsentrasi dan rendemen xilitol pada sistem selterimobilisasi setengah kali lebih rendah daripada sel bebas (kontrol).

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ABSTRACTResearch on adaptation and immobilization method of Candida tropicalisInaCC Y799 in xylitol production from sugarcane (Saccharum officinarum L.)waste hemicellulosic hydrolysate has been conducted. Sugarcane waste werepretreated with 1,8% of maleic acid and microwave at 180 C for 5 minutes. Theaim of this research were to study the effects of yeast adaptation using sugarcanewaste hydrolysate and the potential of using calcium alginate as immobilizationmatrix of yeast C. tropicalis InaCC Y799 in the xylitol production duringfermentation. The results revealed that fermentation using adapted yeast in 75%concentration of hydrolysate produce higher xylitol concentration and yield thanthose with non adapted yeast. The highest xylitol concentration and yield obtainedusing adapted yeast were 11.27 1.65 g/L and 0.56 0.05 g xylitol /g initialxylose (54.98% of theoretical yield) for 24-hours fermentation. However, thehighest xylitol yield obtained by immobilization method were 5.51 0.63 g/L and0.27 0.04 g xilitol/g initial xylose (29.97% of theoretical yield) for 48-hoursfermentation, which were lower than free cells system."

"Penelitian tentang metode freezing (-4° C) dengan menggunakan freezer lemari pendingin untuk preservasi 13 strain Nostoc [Vaucher 1803] Bornet et Flahault 1886 telah dilakukan. Penelitian bertujuan untuk mengetahui kemampuan tumbuh dan respon tumbuh strain Nostoc setelah dipreservasi. Strain Nostoc yang diujikan berumur 15 hari dalam medium BG11 bebas unsur nitrogen (pH 7,2). Preservasi koloni Nostoc menggunakan DMSO 5% selama 1 dan 7 hari. Koloni Nostoc tanpa penambahan DMSO 5% digunakan sebagai kontrol. Masing-masing perlakuan dan kontrol dilakukan dalam dua kali ulangan.Hasil penelitian menunjukkan sebanyak 11 dari 13 strain (84,62%) dapat bertahan dan berhasil tumbuh setelah preservasi. Sebelas strain Nostoc tersebut menunjukkan respon tumbuh yang berbeda-beda. Metode freezing (-4° C) menggunakan freezer lemari pendingin dapat digunakan sebagai metode preservasi strain Nostoc yang sederhana dan mudah.

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The use of freezing method (-4° C) by using freezer refrigerator for preservation thirteen Nostoc [Vaucher 1803] Bornet et Flahault 1886 strains had been studied. The purpose of this study was to assess strains ability to grow and grow response after being preserved. Nostoc strains cultured in BG11 N free agar (pH 7,2) for 15 days were used. The preservation used 5% DMSO for 1 and 7 days. Nostoc colonies without presence of 5% DMSO were used as a control. Each test was carried out in duplicate.

The results showed that 11 of 13 strains (84,62%) were able to survive and grow after treatment. 11 Nostoc strains showed different grow response. Freezing method (-4° C) by using freezer refrigerator can be used as a simple and inexpensive preservation method for Nostoc strains."