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Ditemukan 2 dokumen yang sesuai dengan query
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Marina Ika Irianti
"Candida albicans merupakan salah satu jenis fungi yang dapat menyebabkan infeksi dengan prevalensi yang tinggi di dalam tubuh manusia. Dalam menangani kandidiasis terdapat beberapa kendala sehingga diperlukan metode alternatif untuk menangani infeksi yang disebabkan oleh Candida albicans. Patogenisitas Candida albicans erat kaitannya dengan terbentuknya biofilm. Biofilm adalahkoloni mikroba (biasanya penyebab suatu penyakit) yang membentuk suatu matriks polimer organik. Aptamer merupakan single-strand ribonucleic acid (ssRNA) dan single-strand deoxyribonucleic acid (ssDNA) yang mempunyai afinitas dan spesifisitas yang tinggi.
Tujuan dari penelitian ini adalah untuk mengetahui potensi aptamer dalam menghambat pembentukan biofilm pada beberapa galur Candida albicans. Galur Candida albicans yang dibandingkan antara lain galur ATCC 10231, isolat oral, dan isolat vaginal. Metode yang digunakan adalah pengujian menggunakan MTT untuk menentukan viabilitas sel, pengujian kristal violet untuk menentukan massa biofilm, dan kuantifikasi Candida albicans dengan real-time PCR. Waktu inkubasi yang digunakan dalam pembentukan biofilm adalah 1.5; 24; 48; 72 jam. Pengujian menggunakan MTT, kristal violet, dan Real-Time PCR menunjukkan adanya efek penghambatan biofilm oleh aptamer. Pada waktu inkubasi 48 jam, penambahan aptamer menunjukkan efek penghambatan biofilm secara pengujian MTT dan kristal violet. Terdapat perbedaan yang signifikan pada potensi aptamer dalam menghambat biofilm Candida albicans di waktu inkubasi 72 jam dengan pengujian MTT dan kristal violet (p<0.05).

Candida albicans is a fungal which has high prevalence in human body. There are some drawbacks in C.albicans treatment which is why an alternative method is required to cure infections caused by Candida albicans. Candida albicans pathogenecity closely related on biofilm formation. Biofilms are microbial colonies (usually the cause of a disease) which forms an organic polymer matrix. Aptamers are single-strand ribonucleic acid (ssRNA) and singlestrand deoxyribonucleic acid (ssDNA) which have high affinity and specificity to the target.
The aim of this study is to know potencial of aptamer to inhibit biofilm formation in some strains Candida albicans. There are Candida albicans ATCC 10231, isolate oral, and isolate vaginal. Methods that used in this study are MTT assay to determine cell viability, crystal violet assay to determine biofilm mass, and quantification of Candida albicans by Real-Time PCR. Incubation times that used for biofilm formation are 1,5; 24; 48; 72 hours. There are inhibition effects of aptamer in biofilm formation by MTT assay, crystal violet assay, and Real-Time PCR. In incubation time 48 hours, there are inhibition effect of aptamer in biofilm formation by MTT assay and crystal violet method. There is significance difference of aptamer potential in biofilm formation from crystal violet assay and MTT assay in incubation time 72 hours (p<0.05).
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Depok: Fakultas Farmasi Universitas Indonesia, 2014
S56753
UI - Skripsi Membership  Universitas Indonesia Library
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Marina Ika Irianti
"[ABSTRAK
Badan Pengawas Obat dan Makanan (BPOM) merupakan Lembaga Pemerintah Non Kementerian (LPNK) yang bertanggung jawab kepada Presiden dan melaksanakan tugas pemerintahan di bidang pengawasan Obat dan Makanan sesuai dengan ketentuan peraturan Perundang-Undangan yang berlaku. Kegiatan praktik kerja di Badan Pengawas Obat dan Makanan bertujuan agar calon apoteker : mampu memahami peranan, tugas, dan tanggung jawab apoteker di Badan Pengawas Obat dan Makanan; memiliki pengetahuan tentang tugas pokok dan fungsi Badan Pengawas Obat dan Makanan di bidang farmasi; memiliki wawasan, pengetahuan, keterampilan, dan pengalaman praktis melakukan pekerjaan di Badan Pengawas Obat dan Makanan; memiliki gambaran nyata tentang permasalahan kefarmasian di Badan Pengawas Obat dan Makanan. Mengingat bahwa penyalahgunaan narkotika, psikotropika, dan zat adiktif merupakan tantangan terutama bagi generasi muda di Indonesia, salah satu direktorat di Badan Pengawas Obat dan Makanan (BPOM) yaitu Direktorat Pengawasan NAPZA (Narkotik, Psikotropik, dan Zat Adiktif) bertugas melaksanakan kegiatan pengawasan terhadap Narkotika, Psikotropika, Prekursor, dan Rokok secara komprehensif dari hulu ke hilir. Selain itu, Direktorat Pengawasan NAPZA juga melakukan pengawasan terkait rokok, termasuk produk rokok dan iklan rokok.

ABSTRACT
National Agency of Drug and Food Control is a Non-Government Organization that is responsible to the President and carry out government duties in the field of Food and Drug supervision in accordance with the provisions of the applicable Legislation. Activity in the working practices of Food and Drug Supervisory Agency aims to prospective pharmacists: able to understand the role, duties and responsibilities of pharmacists in the Food and Drug Administration; have knowledge of basic tasks and functions of the Food and Drug Administration in the field of pharmacy; has the insight, knowledge, skills, and practical experience doing the job at the Food and Drug Administration; have knowledge about the problems of pharmacy at the Food and Drug Supervisory Agency. Given that the abuse of narcotics, psychotropic and addictive substances is a challenge, especially for the younger generation in Indonesia, one of the directorates in the National Agency of Drug and Food Control, namely the Directorate of Drugs (Narcotics, Psychotropic and Addictive Substances) in charge of conducting surveillance of Narcotics , Psychotropic Substances, Precursors, and Cigarettes comprehensively from upstream to downstream. In addition, the Directorate of Drug Control also monitors related to cigarettes, including cigarettes and cigarette advertising products.;National Agency of Drug and Food Control is a Non-Government Organization that is responsible to the President and carry out government duties in the field of Food and Drug supervision in accordance with the provisions of the applicable Legislation. Activity in the working practices of Food and Drug Supervisory Agency aims to prospective pharmacists: able to understand the role, duties and responsibilities of pharmacists in the Food and Drug Administration; have knowledge of basic tasks and functions of the Food and Drug Administration in the field of pharmacy; has the insight, knowledge, skills, and practical experience doing the job at the Food and Drug Administration; have knowledge about the problems of pharmacy at the Food and Drug Supervisory Agency. Given that the abuse of narcotics, psychotropic and addictive substances is a challenge, especially for the younger generation in Indonesia, one of the directorates in the National Agency of Drug and Food Control, namely the Directorate of Drugs (Narcotics, Psychotropic and Addictive Substances) in charge of conducting surveillance of Narcotics , Psychotropic Substances, Precursors, and Cigarettes comprehensively from upstream to downstream. In addition, the Directorate of Drug Control also monitors related to cigarettes, including cigarettes and cigarette advertising products., National Agency of Drug and Food Control is a Non-Government Organization that is responsible to the President and carry out government duties in the field of Food and Drug supervision in accordance with the provisions of the applicable Legislation. Activity in the working practices of Food and Drug Supervisory Agency aims to prospective pharmacists: able to understand the role, duties and responsibilities of pharmacists in the Food and Drug Administration; have knowledge of basic tasks and functions of the Food and Drug Administration in the field of pharmacy; has the insight, knowledge, skills, and practical experience doing the job at the Food and Drug Administration; have knowledge about the problems of pharmacy at the Food and Drug Supervisory Agency. Given that the abuse of narcotics, psychotropic and addictive substances is a challenge, especially for the younger generation in Indonesia, one of the directorates in the National Agency of Drug and Food Control, namely the Directorate of Drugs (Narcotics, Psychotropic and Addictive Substances) in charge of conducting surveillance of Narcotics , Psychotropic Substances, Precursors, and Cigarettes comprehensively from upstream to downstream. In addition, the Directorate of Drug Control also monitors related to cigarettes, including cigarettes and cigarette advertising products.]"
2015
PR-PDF
UI - Tugas Akhir  Universitas Indonesia Library