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Abstrak :
ABSTRACT
Jamur endofitik Chrisonilia sitophila, Acremonium sp., dan Penicillium sp. telah diisolasi dari jaringan ranting tumbuhan kandis gajah. Ketiga strain kapang tersebut ditumbuhkan dalam 3 l medium potato dextrose broth (PDB) pada temperatur kamar selama 28 hari. Masing-masing biakan disaring untuk memisahkan miselium dan dilanjutkan dengan ekstraksi dan evaporasi. Semua ekstrak dilakukan uji aktivitas antioksidan berdasarkan aktivitas peredaman radikal bebas 1,1-diphenyl-2-picrylhydrazyl (DPPH). Ekstrak Acremonium sp. memiliki aktivitas yang kuat dengan nilai IC50 10,3μ g/ml yaitu setara dengan aktivitas asam askorbat dengan nilai IC50 9,8μ g/ml. Ekstrak aktif selanjutnya dikromatografi kolom dan diteruskan dengan rekromatografi hingga diperoleh senyawa antioksidan murni berupa minyak bewarna kuning. Struktur molekul ditentukan berdasarkan data spektroskopi yang meliputi 1 H-NMR, 13 C-NMR, HMQC, HMBC, dan COSY. Senyawa hasil isolasi adalah golongan seskuiterpen yaitu 3,5-dihidroksi-2,5-dimetiltrideka-2,9,11-triena-,8-dion.

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Abstract
The endophytic fungi Chrisonilia sitophila, Acremonium sp., and Penicillium sp. have been isolated from the tissues of the twigs of kandis gajah. The fungal strains were grown in 3 l potato dextrose broth medium (PDB) at room temperature for 28 days. To extract the antioxidant compounds, the cultures broth were filtered for mycelia removal followed by extraction and evaporation. All of the extracts were evaluated for their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The extract of Acremonium sp. had strong activity with IC50 value of 10.3μ g/ml, which is equivalent to ascorbic acid activity with IC50 value of 9.8μ g/ml. The extract was subjected to column chromatography on Si gel twice to obtain a high purity antioxidant compound in the form of yellow oil. The molecular structure was determined based on spectroscopic data, including 1 H-NMR, 13 C-NMR, HMQC, HMBC, and COSY. The compound was determined as sesquiterpene 3,5-dihydroxy-2,5-dimethyltrideca-2,9,11-triene-4,8-dione.

Abstrak :
The endophytic fungi Aspergillus fumigatus was isolated from the tissues of the fruits of Garcinia griffithii. The fungal strain was identified from the colony, and it was characteristic of cell morphol ogy. The ethyl acetate extracts derived from fungus cultures showed major spots on TLC under UV light, which was continued to the isolation of the secondary metabolites. The structure of the isolated compound was elucidated on the basis of NMR analyses (1H-NMR, 13C-NMR, HMQC, HMBC and H-H COSY). The compounds were identified as: 4,6-dihydroxy, 3,8a-dimethyl-1-oxo-5-(3?-oxobutan-2?-yl)-1,4,4a,5,6,8a-hexahydronaphthalen-2-yl-1?,2?-dimethyl-5?-(2??-methylprop-1??-enyl)cyclopentane-carboxylate.

Abstrak :
Garcinia griffithii and Garcinia cowa belong to the genus Garcinia. The genus Garcinia has been known to be a rich source of secondary metabolites, such as xanthones, benzophenones, flavonoids, steroids, terpenoids, and other phenolic derivatives. Previous investigations of endophytic fungi from G. griffithii revealed the presence of three compounds not found in the host. In order to the continue the phytochemical work on endophytic fungi of G. griffithii, the constituent of the endophytic fungi of G. griffithii was re-examined. In this study, a benzoyl compound similar to that found in the endophytic fungus of G. cowa was observed. The same benzoyl compound was also isolated from the endophytic fungus Acremonium sp of G. griffithii and Aspergillus sp of G. cowa with cultivation of eight weeks in static conditions at room temperature. The culture medium was partitioned using ethyl acetate and evaporated to obtain the concentrated extract. Isolation of compounds was performed using the chromatography method. The chemical structure was proposed on the basis of spectroscopic data, including ultraviolet (UV), infrared (IR), mass spectrometry (MS), proton nuclear magnetic resonance (1H-NMR), carbon nuclear magnetic resonance (13C-NMR), heteronuclear single-quantum correlation spectroscopy (HSQC), heteronuclear multiple-bond correlation spectroscopy (HMBC), and correlation spectroscopy (COSY).

Suatu Senyawa Benzoil Baru Hasil Isolasi dari Jamur Endofit Tumbuhan Kandis Gajah (Garcinia griffithii) dan Asam Kandis (Garcinia cowa). Tumbuhan Garcinia griffithii dan Garcinia cowa termasuk ke dalam genus Garcinia. Genus Garcinia dikenal kaya dengan kandungan metabolit sekunder, seperti santon, benzofenon, flavonoid, steroid, terpenoid, dan turunan fenolik lainnya. Penelitian sebelumnya terhadap jamur endofit dari G. griffithii telah menemukan tiga senyawa yang tidak ditemukan dalam tumbuhan inangnya. Sebagai lanjutan dari penelitian fitokimia mengenai jamur endofitik tumbuhan G. griffithii, kandungan kimia dari jamur endofitik ini kembali diteliti. Dalam studi ini, juga diperoleh senyawa benzoil yang sama dengan yang ditemukan dalam jamur endofit tumbuhan G. Cowa. Senyawa benzoil yang sama juga dapat diisolasi dari jamur Acremonium sp tumbuhan G. griffithii dan dari jamur Aspergillus sp tumbuhan G. cowa dengan masa kultivasi delapan minggu dalam kondisi statis pada suhu kamar. Medium kultur dipartisi menggunakan etil asetat dan dievaporasi untuk mendapatkan ekstrak pekatnya. Isolasi senyawa dilakukan dengan metode kromatografi. Struktur kimia diusulkan berdasarkan data spektroskopi yang meliputi ultraviolet (UV), infrared (IR), mass spectrometry (MS), proton nuclear magnetic resonance (1H-NMR), carbon nuclear magnetic resonance (13C-NMR), heteronuclear single-quantum correlation spectroscopy (HSQC), heteronuclear multiple-bond correlation spectroscopy (HMBC), dan correlation spectroscopy (COSY).