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Dodin Koswanudin
"ABSTRACT
The Asian corn borer, Ostrinia furnacalis (Guenee) (Lepidoptera: Crambidae), is one of the most serious insect pests of maize in Indonesia, Japan, China and other Asian countries. The insect is not easily controlled by insecticides because the larvae develop and hide in the corn stem. The application of pheromone usually aims to control insect pests, to monitor its occurrence, or for mass trapping. Sex pheromone components of this spesies has been intensively studied in Japan and China. However, in Indonesia this has not been extensively explored yet. Samudra et al. (2006, unpublished) analyzed sex pheromone gland extracts of O. furnacalis from Bogor (Indonesia) by gas chromatography (GC) and GC combined with mass spectrometry (GC-MS) and identified as (Z)-12- tetradecenyl acetate and (E)-12-tetradecenyl acetate at ratio of about 62 : 38.
In this research, we studied the attractiveness of (Z)-12- tetradecenyl acetate and (E)-12-tetradecenyl acetate at different ratios and quantities in a laboratory wind-tunnel bioassay and field experiments. The percentages (ratios) of Z-12-tetradecenyl acetate tested were 100, 90, 65, 50, 35, 10 and 0 % in 100 μg mixture of Z- and E-12-tetradecenyl acetate. The quantities of mixture of two components tested were 50, 100, 250, 500,1000 ug per rubber septum.
An indoor wind-tunnel (30 cm in diam. x 200 cm) was used to test the biological activities of the chemicals, and the experiment was carried out in a scotophase (a dark period). Four criteria were used to judge the behavioral responses of the insect :1. starting flight, 2. orientation, 3. reaching the source, and 4. landing on the source. Semi-field experiment was carried out in a screen house (20 m x 6 m). One hundred males of one day old moth, were released in screen house and number of males caught in each trap were recorded in three days. Field experiments were conducted in sweet corn fields at Bogor and Bandung, West Java. Number of male(s) caught in each trap were recorded every three days during 54 days. The experiment was aranged in a complete randomize designed and the data were analysed with ANOVA and subjected to HSD test.
The results of wind tunnel bio-assay showed that the mixture of (Z) and (E)-12-tetradecenyl acetate with ratio 65 : 35 was the most attractive, 60 ? 100% of tested males landed on pheromone source. The semi-field experiment, it also showed that the pheromone mixture of (Z)- and (E)-12-tetradecenyl acetate with ratio 65 : 35 caught more males, for both corn borer populations from Bogor and Bandung. The best quantity of pheromone was showed by 500 μg/rubber septum with ratio 65 : 35 (Z : E)-12-tetradecenyl acetate. Field experiment data in Bogor and Bandung showed that binary blend of synthetic (Z)- and (E)-12-tetradecenyl acetate with ratio 65 : 35 was significantly more attractive than 3 virgin females. Insect caught in trap increased with the increase of amount of pheromone sex in a range of 50 to 500μg/ rubber septum. There was less males caught in the treatment of 1000 μg/rubber septum, both in Bogor and Bandung field experiments.
Based on laboratory and field trap experiments, we conclude that the sex pheromone of O. furnacalis, composed of (Z)-12-teradecenyl acetate and (E)-12-tetradecenyl acetate at ratio 65 : 35, is the most attractive to the asian corn borer. The binary blend at amount 500 μg/rubber septum attracted nearly three times more males than did by virgin females"
[, ], 2008
T39489
UI - Tesis Open  Universitas Indonesia Library
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Dodin Koswanudin
"[ABSTRAK
Telah dilakukan penelitian bioekologi lalat buah (Bactrocera carambolae) (Drew and Hancock) dan (Bactrocera papayae) (Drew and Hancock) selama satu tahun (Februari 2011 ? Januari 2012) di Taman Buah Mekarsari, Cileungsi, Bogor, Jatibarang, Indramayu dan
Pancoran Mas, Depok, Provinsi Jawa Barat. Kegiatan yang dilakukan meliputi penelitian sebaran populasi lalat buah B. carambolae dan B. papayae di lapangan, uji preferensi lalat buah di laboratorium, dan parasitasi parasitoid lalat buah pada tanaman belimbing, mangga, jambu bol, jambu air dan jambu biji. Penelitian sebaran populasi lalat buah dilakukan dengan cara pemasangan perangkap botol bekas air mineral yang beratraktan metil eugenol (ME) dan digantungkan pada tanaman inang ketinggian 2 m. Populasi lalat buah yang tertangkap dalam perangkap diamati dua kali dalam seminggu. Penelitian preferensi tanaman inang terhadap lalat buah dilakukan di laboratorium pada alat ?winntunel?, lalat buah betina dimasukkan dalam dalam sangkar lalat buah di bagian tengah yang dihubungkan dengan toples wadah buah inang di sekelilingnya. Melalui udara yang dialirkan dari toples buah ke sangkar lalat buah akan menarik lalat buah untuk memilih buah inang yang paling disukai. Peubah yang diamati meliputi jumlah lalat buah yang masuk, kerusakan buah, jumlah pupa dan perbandingan kelamin, pengamatan dilakukan setiap hari. Penelitian parasitasi parasotid lalat buah dilakukan dengan cara mengumpulkan buah-buah yang terserang sebanyak 30 buah/tanaman dibawa ke laboratorium disimpan secara terpisah satu buah dalam satu wadah toples. Buah-buah dipelihara sampai muncul imago lalat buah dan parasitoid yang muncul, diamati jenis parasitoid dan tingkat parasitasinya. Hasil penelitian menunjukkan bahwa populasi lalat buah B. carambolae di Mekarsari sangat tinggi diperoleh pada belimbing Malaya, jambu biji Getas merah, jambu bol Jamaica, jambu air Citra, sementara di Indramayu sangat tinggi pada mangga Harumanis, dan di Depok populasi tertinggi pada belimbing Dewi. Populasi B. papayae tertinggi di Mekarsari pada mangga Manalagi, di Indramayu pada mangga Harumanis dan di Depok pada belimbing Dewi. Baik populasi B. carambolae maupun B. papayae tampak rendah pada belimbing di Welahan. Iklim memengaruhi fluktuasi populasi B. carambolae dan B. papayae. Preferensi dan perkembangan B. carambolae tertinggi pada belimbing Malaya diikuti mangga Manalagi dan untuk B. papayae pada pepaya California yang diikuti mangga Manalagi. Penelitian ini juga menunjukkan bahwa preferensi dan perkembangan B. carambolae, B. papayae sangat rendah pada varietas belimbing Welahan. Parasitoid yang menyerang B. carambolae dan B. papayae di tiga tempat penelitian didominasi oleh dua spesies, yaitu, Diachasmimorpha longicaudata, dan Fopius arisanus (Hymenoptera: Braconidae). Tingkat parasitasi D. longicaudata lebih tinggi (21,9 ? 41,6%) dibandingkan F. arisanus (16,1 ? 38,9%) pada semua tanaman inang yang diamati;

ABSTRACT
Bio-ecology research on fruit flies (Bactrocera carambolae) (Drew and Hancock), and (Bactrocera papayae) (Drew and Hancock) were conducted for one year (February 2011 - January 2012) at three different areas: The Fruit Garden Mekarsari, Cileungsi, Bogor, Jatibarang, Indramayu and Pancoran Mas , Depok, all in West Java Province. The activities included studies on dispersal of fruit flies population B. carambolae and B. papayae in the field, and preferences tests of fruit flies in laboratory, and parasitic of parasitoids of fruit flies on star fruits, mangos, guava bol, water guavas and stone guavas. The studies of dispersal of Fruit flies population distribution were conducted at the above three named areas, using used bottles of mineral water as traps containing methyl eugenol (ME) hung on the host plants at 2 m height. The Fruit flies populations trapped in the used mineral water bottles were observed twice a week. The research preference of host plants for fruit flies were conducted at the laboratory using wind tunnel equipment, and the female of fruit flies were placed in the middle of flies cages and connected to the surrounding host fruit containers. Through the air flow from containers to the cages fruit flies were attracted to choose the most preferred host fruits. The measured variables included the trapped amount of fruit flies, damage of fruits, pupa number and sex ratios, and the observations were conducted daily . The studies of parasicity of fruit flies parasitoid were conducted by collecting approximately 30 attacked fruits from each plant and kept individually in the laboratory. The fruits were retained until the imago fruit flies and parasitoids emerged, then the type of parasitoids and their level parasitoicity wer observed. The results showed that the fruit flies population B. carambolae was very high at Mekarsari derived from Malaya star fruits, guava Getas Merah, jambu bol Jamaica, water guava Citra, while the high level of population at Indramayu was observed on Harumanis mangos, and in Depok was on star fruits Dewi. The population of B. carambolae and B.papayae appeared to below on star fruits at Welahan. Climatic fluctuations affected the population of B. carambolae and B. papayae. The preference and the regeneration of B. carambolae were higher on Malaya star fruits followed by mango Manalagi (a variety of Mango) and B. papayae was from the California variety, followed by mango Manalagi. The study also showed that the preferences and regenerations of B. carambolae, B. papayae were very low on star fruit varieties Welahan. The domination of Parasitoids on B. carambolae and B. papayae attacks in the three research locations were two species namely, Diachasmimorpha longicaudata and Fopius arisanus (Hymenoptera: Braconidae). The parasitoicity level of D. longicaudata was higher (21.9 to 41.6%) compared to F. arisanus (16.1 to 38.9%) on all of the observed host plants.;Bio-ecology research on fruit flies (Bactrocera carambolae) (Drew and Hancock), and (Bactrocera papayae) (Drew and Hancock) were conducted for one year (February 2011 - January 2012) at three different areas: The Fruit Garden Mekarsari, Cileungsi, Bogor, Jatibarang, Indramayu and Pancoran Mas , Depok, all in West Java Province. The activities included studies on dispersal of fruit flies population B. carambolae and B. papayae in the field, and preferences tests of fruit flies in laboratory, and parasitic of parasitoids of fruit flies on star fruits, mangos, guava bol, water guavas and stone guavas. The studies of dispersal of Fruit flies population distribution were conducted at the above three named areas, using used bottles of mineral water as traps containing methyl eugenol (ME) hung on the host plants at 2 m height. The Fruit flies populations trapped in the used mineral water bottles were observed twice a week. The research preference of host plants for fruit flies were conducted at the laboratory using wind tunnel equipment, and the female of fruit flies were placed in the middle of flies cages and connected to the surrounding host fruit containers. Through the air flow from containers to the cages fruit flies were attracted to choose the most preferred host fruits. The measured variables included the trapped amount of fruit flies, damage of fruits, pupa number and sex ratios, and the observations were conducted daily . The studies of parasicity of fruit flies parasitoid were conducted by collecting approximately 30 attacked fruits from each plant and kept individually in the laboratory. The fruits were retained until the imago fruit flies and parasitoids emerged, then the type of parasitoids and their level parasitoicity wer observed. The results showed that the fruit flies population B. carambolae was very high at Mekarsari derived from Malaya star fruits, guava Getas Merah, jambu bol Jamaica, water guava Citra, while the high level of population at Indramayu was observed on Harumanis mangos, and in Depok was on star fruits Dewi. The population of B. carambolae and B.papayae appeared to below on star fruits at Welahan. Climatic fluctuations affected the population of B. carambolae and B. papayae. The preference and the regeneration of B. carambolae were higher on Malaya star fruits followed by mango Manalagi (a variety of Mango) and B. papayae was from the California variety, followed by mango Manalagi. The study also showed that the preferences and regenerations of B. carambolae, B. papayae were very low on star fruit varieties Welahan. The domination of Parasitoids on B. carambolae and B. papayae attacks in the three research locations were two species namely, Diachasmimorpha longicaudata and Fopius arisanus (Hymenoptera: Braconidae). The parasitoicity level of D. longicaudata was higher (21.9 to 41.6%) compared to F. arisanus (16.1 to 38.9%) on all of the observed host plants., Bio-ecology research on fruit flies (Bactrocera carambolae) (Drew and Hancock), and (Bactrocera papayae) (Drew and Hancock) were conducted for one year (February 2011 - January 2012) at three different areas: The Fruit Garden Mekarsari, Cileungsi, Bogor, Jatibarang, Indramayu and Pancoran Mas , Depok, all in West Java Province. The activities included studies on dispersal of fruit flies population B. carambolae and B. papayae in the field, and preferences tests of fruit flies in laboratory, and parasitic of parasitoids of fruit flies on star fruits, mangos, guava bol, water guavas and stone guavas. The studies of dispersal of Fruit flies population distribution were conducted at the above three named areas, using used bottles of mineral water as traps containing methyl eugenol (ME) hung on the host plants at 2 m height. The Fruit flies populations trapped in the used mineral water bottles were observed twice a week. The research preference of host plants for fruit flies were conducted at the laboratory using wind tunnel equipment, and the female of fruit flies were placed in the middle of flies cages and connected to the surrounding host fruit containers. Through the air flow from containers to the cages fruit flies were attracted to choose the most preferred host fruits. The measured variables included the trapped amount of fruit flies, damage of fruits, pupa number and sex ratios, and the observations were conducted daily . The studies of parasicity of fruit flies parasitoid were conducted by collecting approximately 30 attacked fruits from each plant and kept individually in the laboratory. The fruits were retained until the imago fruit flies and parasitoids emerged, then the type of parasitoids and their level parasitoicity wer observed. The results showed that the fruit flies population B. carambolae was very high at Mekarsari derived from Malaya star fruits, guava Getas Merah, jambu bol Jamaica, water guava Citra, while the high level of population at Indramayu was observed on Harumanis mangos, and in Depok was on star fruits Dewi. The population of B. carambolae and B.papayae appeared to below on star fruits at Welahan. Climatic fluctuations affected the population of B. carambolae and B. papayae. The preference and the regeneration of B. carambolae were higher on Malaya star fruits followed by mango Manalagi (a variety of Mango) and B. papayae was from the California variety, followed by mango Manalagi. The study also showed that the preferences and regenerations of B. carambolae, B. papayae were very low on star fruit varieties Welahan. The domination of Parasitoids on B. carambolae and B. papayae attacks in the three research locations were two species namely, Diachasmimorpha longicaudata and Fopius arisanus (Hymenoptera: Braconidae). The parasitoicity level of D. longicaudata was higher (21.9 to 41.6%) compared to F. arisanus (16.1 to 38.9%) on all of the observed host plants.]"
2015
D2098
UI - Disertasi Membership  Universitas Indonesia Library