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"ABSTRAKLatar Belakang: Gel APF yang bersifat asam dapat meningkatkan kekasaranpermukaan bahan restorasi, yang menyebabkan adhesi bakteri dan karies sekunder.Tujuan: Menganalisis pengaruh aplikasi gel APF terhadap kekasaran permukaanresin sealant. Metode: Tiga puluh enam spesimen resin sealant dibagi secara acakke dalam enam kelompok, yaitu aplikasi gel APF dan akuades selama tiga puluhmenit sebanyak satu, dua dan tiga kali. Nilai kekasaran permukaan rerata diukurdengan surface roughness tester Mitutoyo SJ201. Hasil: Kekasaran permukaanmeningkat bermakna setelah satu kali aplikasi, dan menurun bermakna setelah duakali aplikasi. (p<0,05)Kesimpulan: Gel APF menurunkan kekasaran permukaanresin sealant setelah dua kali aplikasi.

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ABSTRACTBackground: Acidity of APF gel could increase restorative material surfaceroughness, leading to increased bacterial adhesion and secondary caries. Aim: Toanalyze the effect of APF gel application on the surface roughness of resin sealant.Method: Thirty six resin sealant specimens were randomly divided into six groups,APF gel and aquadest one, two and three times application for thirty minutes each.Mean roughness was measured by Mitutoyo SJ201 surface roughness tester. Result:Surface roughness was significantly increased after one time gel application, anddecreased significantly after twice gel application. (p<0,05)Conclusion: APF geldecreased resin sealant surface roughness after second application."

"Latar Belakang: L-arginin merupakan asam amino semiesensial yang produksinya tidak mencukupi kebutuhan dalam kondisi stres oksidatif akibat inflamasi. L-arginin adalah satu-satunya substrat bagi enzim nitric oxide synthase (NOS) yang memproduksi nitric oxide (NO) yang dapat mengaktivasi focal adhesion kinase (FAK) pathwaydan memicu terjadinya proses migrasi sel. Tujuan: Mengetahui potensi media kultur asam amino L-arginin terhadap laju kecepatan migrasi hDPSCs. Metode: Evaluasi media kultur asam amino L-arginin konsentrasi 300, 400, 500 ¼mol/L, serta DMEM sebagai kontrol terhadap laju kecepatan migrasi hDPSCs menggunakan uji scratch assay menggunakan uji scratch assay yang dihitung dengan rumus laju kecepatan migrasi setelah 24 jam. Analisis statistic menggunakan Paired T-Test dan Oneway ANOVA dengan post hoc LSD. Hasil: Terdapat perbedaan bermakna potensi L-arginin 500 μmol/L dibandingkan konsentrasi 300 dan 400 μmol/L, serta kontrol. Kesimpulan: Media kultur asam amino L-arginin 500 ¼mol/L memiliki potensi laju kecepatan migrasi yang lebih baik dibandingkan konsetrasi 300, 400 ¼mol/L dan kontrol.

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Background: L-arginine is semiessential amino acid which the production is insufficient under oxidative stress due to inflammation. L-arginine is the only substrate of nitric oxide synthase (NOS) enzyme that produces nitric oxide (NO) which activates focal adhesion kinase (FAK) pathway to stimulate cell migration.

Objective: To understand potential of L-arginine amino acid culture media towards speed rate of hDPSCs migration.

Methods: Evaluation of 300, 400, 500 ¼mol/L of L-arginin amino acid culture media and DMEM as control towars speed rate of hDPSCs migration using scratch assay and calculation of migration speed rate after 24 hours. Statistical analysis using Paired T-Test and Oneway ANOVA with post hoc LSD.

Results: Significant result was shown between 500 ¼mol/L of L-arginin amino acid culture media compared with 300 and 400 ¼mol/L concentration and control towards migration speed rate after 24 hours.

Conclusion: 500 ¼mol/L of L-arginin amino acid culture media has a better migration rate compared with lower concentrations and control."