Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Tumbuhan sukun (Artocarpus altilis) adalah salah satu tumbuhan nangkanangkaan (Artocarpus) dari suku Moraceae yang dikenal baik di Indonesia, merupakan tanaman asli Asia Selatan, Asia Tenggara, New Guinea dan Pasifik Selatan. Dua senyawa berhasil di isolasi dari ekstrak etil asetat daun sukun (Artocarpus altilis) yaitu β-Sitosterol yang berupa kristal jarum berwarna putih mempunyai titik leleh 133-135 ˚C yang memiliki massa molekul 414 dan suatu senyawa JS7 turunan flavonoid berupa padatan kuning cerah dengan massa molekul 404, Senyawa tersebut dapat dipisahkan dengan menggunakan metode ekstraksi secara maserasi, fraksinasi dengan berbagai cara kromatografi, dan pemurnian dengan cara kristalisasi menggunakan sistem dua pelarut. Struktur molekul ditentukan dengan memanfaatkan data fisika dan spektroskopi UV, IR dan 1H dan 13C-NMR. Uji antioksidan menggunakan metode radical scavenger terhadap DPPH menujukkan senyawa turunan flavonoid JS7 kurang aktif sebagai antioksidan dengan IC50 137,00 µg/mL dan uji antikanker menggunakan sel leukemia L-1210 cukup potensial sebagai antikanker dengan IC50 sebesar 5,12 µg/mL. ......Breadfruit (Artocarpus altilis) is one of the Moraceae family well known in Indonesia, is a native plant of South Asia, Southeast Asia, New Guinea and the South Pacific. Two compounds successfully were isolated from the ethyl acetate extract of breadfruit leaves (Artocarpus altilis), there are β-sitosterol (white needle crystals ) with melting point of 133 -135 ˚ C, which has a molecular mass of 414 and one derivative of flavonoid compounds JS7 a bright yellow solid with a molecular mass of 404, these compounds were separated by maceration extraction, fractionation and isolation on chromatography, and purification methods. Crystallization was done by using two solvent systems. The molecular structure were determined by utilizing the physical and spectroscopic data of UV, IR and 1H and 13C-NMR.The isolated compound showed antioxidant activity in DPPH method, JS7 flavonoid derivative compound showed less active as an antioxidant with IC 50 = 137,00 ug / mL and anti-cancer test using L-1210 live cell leukemin a cell exhibited a significant against L1210 cell live with IC50 = 5,12 gram/mL.

Abstrak :
Penelitian bertujuan mengetahui fase pertumbuhan Chlorella sp.; identitas molekuler Chlorella sp.; mendeteksi lipid Chlorella sp. secara kualitatif; waktu panen Chlorella sp. dengan biomassa terbanyak; waktu panen Chlorella sp. dengan lipid terbaik; dan mengkarakterisasi asam lemak pada tiga fase pertumbuhan Chlorella sp. Sepuluh mikroalga diseleksi menjadi tiga melalui identifikasi molekuler, pendeteksian lipid kualitatif menggunakan nile-red, dan pengamatan pertumbuhan dalam Erlenmeyer, selanjutnya karakterisasi pertumbuhan, pengamatan produksi biomassa dengan metode filtrasi, penghitungan kadar lipid dengan ekstraksi Bligh-Dyer, dan analisis profil asam lemak dengan karakterisasi FAME menggunakan GCMS. Hasil penelitian menunjukkan Chlorella sp. MA-84, Chlorella sp. MA-86, dan Chlorella sp. MA-90 diidentifikasi secara molekuler sebagai Chlorella vulgaris dan memiliki potensi lipid terbaik secara kualitatif tanpa hambatan pertumbuhan biomassa. Terdapat variasi fase-fase pertumbuhan pada ketiga strain. Chlorella vulgaris MA-84, Chlorella vulgaris MA-86, dan Chlorella vulgaris MA-90 menghasilkan biomassa tertinggi masing-masing 1,242±0,08 g/L (t12), 3,217±0,17 g/L (t14), dan 0.604±0.04 g/L (t16), kadar lipid tertinggi masing-masing 13,853±7,09% (t15), 26,810±22,62% (t16), dan 10,161±3,74% (t16). Ketiga strain mengandung asam palmitat, asam palmitoleat, asam heksadekadienoik, asam linoleat, asam stearat, dan asam arakidat. ......The study aims to identify the growth phases of Chlorella sp., molecularly identify of Chlorella sp., qualitatively detect Chlorella sp. lipids, determine the best harvesting time for maximum biomass and maximum lipid content, also characterize the fatty acids on three growth phases of Chlorella sp. Ten microalgae were selected into three strains through molecular identification, qualitative lipid detection using nile-red, and growth observation in Erlenmeyer, for analyze growth characterization, biomass production using filtration method, lipid content using Bligh-Dyer extraction, and fatty acid profiling through FAME characterization using GCMS. The research results showed that Chlorella sp. MA-84, Chlorella sp. MA-86, and Chlorella sp. MA-90 were molecularly identified as Chlorella vulgaris and demonstrated the best qualitative lipid potential with good growth. There were variations in growth phases among the three strains. Chlorella vulgaris MA-84, Chlorella vulgaris MA-86, and Chlorella vulgaris MA-90 produced the highest biomass of 1.242±0.08 g/L (t12), 3.217±0.17 g/L (t14), and 0.604±0.04 g/L (t16), respectively, and exhibited the highest lipid content of 13.853±7.09% (t15), 26.810±22.62% (t16), and 10.161±3.74% (t16), respectively. All three strains contained palmitic acid, palmitoleic acid, hexadecadienoic acid, linoleic acid, stearic acid, and arachidic acid.