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"Peridontitis kronis adalah salah satu penyakit gigi dan mulut dengan prevalensi terbesar di Indonesia . Bakteri utama penyebab periodontitis kronis adalah bakteri black pigmented, yaitu Porphyromonas gingivalis dan Prevotella intermedia. Binahong telah terbukti secara ilmiah memiliki zat antibakteri. Penelitian ini bertujuan untuk membuktikan efek antibakteri infusum daun binahong terhadap bakteri black pigmented. Konsentrasi infusum yang diuji 100%, 95%, 80%, 65%, dan 50%. Uji difusi cakram kertas dilakukan untuk mengetahui zona hambatan menggunakan media agar brucella Dari uji difusi pada media agar brucella didapatkan nilai zona hambatan sebesar : 0,42 mm (50%), 1,21 mm (65%), 1,18 mm (80%), 1,19 mm (95%) dan 1,36 (100%). Infusum daun binahong memiliki daya hambat terhadap pertumbuhan bakteri black pigmented.

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Chronic Periodontitis is one of the oral disease with highest prevalence in Indonesia. The major periodonthopathogens bacteria in chronic periodontics is black pigmented bacteria, which consist of Porphyromonas gingivalis and Prevotella intermedia. Binahong has been proved scientifically to have antimicrobial substance. This study is aimed to prove that ninahong leaves infusion is effective as an antibacterial agent against black pigmented bacteria in vitro. The concentration of infusion that were used in this test were 100%, 95%, 80%, 65% and 50%. Blank disc diffusion method was performed to measure zone of inhibition in brucella agar. From the diffusion test on brucella agar, the scores of inhibitory zones are : 0,42 mm (50%), 1,21 mm (65%), 1,18 mm (80%), 1,19 mm (95%) dan 1,36 (100%). It is concluded that binahong leaves infusion have inhibition effect on the growth of black pigmented bacteria."

"Tujuan penelitian ini menganalisis efek antibakteri infusum daun Binahong terhadap bakteri Black pigmented dan hubungan antara durasi pemaparan infusum terhadap efek antibakterinya. Efek antibakteri dianalisis melalui nilai Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM). Media yang digunakan adalah Brucella broth dengan darah dan Kanamycin.Infusum diencerkan sampai konsentrasi 20%, 30%, 40%, 50%, 65%, 80%, dan 95% kemudian ditambahkan bakteri 105 cfu/ml lalu diinkubasi selama 24,48, dan 72 jam. Hasil inkubasi kemudian digores padaBrucella agar lalu diinkubasi selama 24 jam. Hasil penelitian menunjukkan KHM >65%, KBM 80% dan tidak terdapat korelasi antara durasi pemaparan infusum daun Binahong terhadap efek antibakterinya.

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This study examines the antibacterial effect of Binahong leaves infusion against Black pigmented bacteria and the correlation between duration of infusion exposure and its antibacterial efficacy. The antibacterial efficacy was analyzed by defining the MIC and MBC. The medium used was Brucella broth containing blood and Kanamycin. Infusion was diluted until 20%,30%,40%,50%,65%,80%,95% concentrations, added with 105 cfu/ml bacteria and incubated for 24,48,72 hours. Each culture were streak on Brucella agar and incubated for 24 hours. Results shows that theMIC was >65%, the MBC was 80% and there were no correlation between duration of infusion exposure and its antibacterial efficacy."

"Latar Belakang: Xanthorrhizol dalam minyak atsiri temulawak memiliki efek antibakteri dan minyak atsiri tersebut berpotensi sebagai bahan dasar antibakteri dalam sediaan obat kumur. Tujuan: menguji efek antibakteri minyak astiri temulawak terhadap Streptococcus mutans. Metode: S. mutans ATCC 25175 dibiakkan dalam medium cair TYS20B selama 3 x 24 jam dipaparkan dengan delapan konsentrasi minyak atsiri yang berbeda selama 24 dan 48 jam dan menguji efek antibakteri dengan metode dilusi. Hasil: kadar hambat minimum minyak atsiri temulawak terlihat pada konsentrasi 35% sedangkan kadar bunuh minimum pada konsentrasi 50%. Kesimpulan: minyak atsiri temulawak memiliki efek antibakteri terhadap S. mutans.

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Background: Xanthorrhizol contained in Curcuma xanthorrhiza Roxb essential oil.has an antibacterial effect and its essential oil is potentially to be an antibacterial basic ingredients in mouthwash.

Objective: to analyze the antibacterial effect in Curcuma xanthorrhiza Roxb. essential oils is tested to Streptococcus mutans.

Method: S.mutans ATCC 25175 are cultured in TYS20B broth medium for 3 x 24 hours. An antibacterial activity tested by dilution method.

Results: the MIC of essential oil was seen in 35% where the MBC was 50%.

Conclusion: essential oils Curcuma xanthorrhiza Roxb. has antibacterial effect against S. mutan."

"Latar Belakang: Demineralisasi dentin akibat karies menyebabkan hilangnya mineral dan serabut protein dari dentin intertubular dan peritubular. Silver memiliki efek bakterisidal dan dalam bentuk silver nano memiliki cakupan kontak permukaan yang lebih baik. Propolis memiliki aktivitas mikroba. Penggabungan silver, silver nano, dan propolis dengan fluoride merupakan suatu keuntungan dan menjadi inovasi baru dalam upaya pencegahan karies. Tujuan: Menganalisis kerja dari SDF, SNF, dan PPF dengan konsentrasi yang berbeda terhadap daya hambat demineralisasi dilihat dari morfologi permukaan dentin dan peningkatan kekerasan dentin. Metode: Dilakukan demineralisasi pada 105 sampel dentin disc 70 sampel morfologi permukaan; 35 sampel kekerasan selanjutnya diaplikasikan SDF Ag 25,4 F- 4,48 ; NSF Ag 1,4 F- 2.26 , NSF Ag 1,9 F- 2,26 ; PPF Propolis 6,67 F- 1,19 , PPF Propolis 10 F- 1,19 yang diikuti proses pH-cycling, morfologi permukaan dentin diobservasi menggunakan alat Scanning Electron Micrograph, Vickers Microhardness Tester untuk menganalisis kekerasan dentin. Hasil: Terdapat gambaran kristal fluorapatit pada permukaan dentin SDF Ag 25,4 F- 4,48 dan NSF Ag 1,4 F- 2.26 , NSF Ag 1,9 F- 2,26 . Terdapat lapisan amorf pada permukaan dentin PPF Propolis 10 F- 1,19. Terdapat peningkatan nilai kekerasan dentin gigi pada seluruh konsentrasi SDF, NSF, dan PPF dengan peningkatan terbesar terjadi pada PPF Propolis 6,67 F- 1,19. Simpulan: SDF, NSF, dan PPF dapat menghambat demineralisasi serta dapat meningkatkan kekerasan dentin walaupun peningkatan tersebut tidak dapat mengembalikan kekerasan awal dentin.

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Background: Dentin demineralization due to caries causes loss of mineral and protein fiber in peritubular dentin and intertubular dentin. Silver has a bactericidal effect, silver nano has a broader contact surface and propolis is an antimicroba agent. The combination of silver, silver nano, and propolis with fluoride is a new innovation in caries prevention.

Aim: To analyze the effect of SDF, SNF, and PPF with different concentration towards inhibitory effect of demineralization through observed from dentine morphology and increase in hardness.

Methods: 105 samples of dentin disc were demineralised and allocated to surface morphology group 70 samples and hardness group 35 samples. Each group received topical application of SDF Ag 25,4 F 4,48 NSF Ag 1,4 F 2.26, NSF Ag 1,9 F 2,26 PPF Propolis 6,67 F 1,19, PPF Propolis 10 F 1,19, followed by pH cycling process, surface morphology of dentin was observed by Scanning Electron Micrograph, Vickers Microhardness Tester was used to analyze dentine hardness.

Result: The surface morphology under SEM showed crystal fluorapatite on the dentin surface of SDF Ag 25,4 F 4,48 , NSF Ag 1,4 F 2.26 , and NSF Ag 1,9 F 2,26 . There was an amorphous layer on the dentin surface of Propolis 10 F 1,19 . Increased of dentin hardness were observed after application of SDF, NSF, and PPF with the highest increase of hardness was in the PPF Propolis 6,67 F 1,19.

Conclusion: SDF, NSF, and PPF can inhibit demineralization of dentin and increase dentin hardness, although cannot restore the initial value of dentin hardness. "

"ABSTRAKLatar belakang: Prakiraan usia untuk usia remaja dan dewasa muda penting dalam konteks hukum dan medikolegal. Pada periode usia ini hanya gigi molar tiga yang masih mengalami proses perkembangan.Tujuan: mengetahui korelasi antara usia kronologis dengan perkembangan gigi molar tiga pada orang Indonesia menggunakan aplikasi metode Demirjian.Metode: Jumlah sampel terdiri dari 407 radiograf panoramik orang Indonesia yang telah diketahui usia kronologis (8-25 tahun). Analisis atatistik menggunakan uji korelasi Pearson. Analisis regresi dilakukan untuk mendapatkan rumus regresi untuk perhitungan prakiraan usia.Hasil: Hasil uji korelasi Pearson, korelasi antara masing-masing gigi molar tiga dengan usia sangat kuat (> 0,75) dan (P<0,05). Didapatkan hubungan regresi model penjumlahan empat gigi molar tiga, tiga gigi molar tiga, dua gigi molar tiga dan satu gigi molar tiga.Kesimpulan terdapat korelasi yang sangat kuat antara usia kronologis dengan perkembangan gigi molar tiga pada orang Indonesia.

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ABSTRACT

Background: Age estimation to adolescence and young adults is important in the context of law and medicolegal. At this age period only third molars are still a process of development.

Objective: To know the correlation between chronological age with the development of the third molars in Indonesia using Application method of Demirjian.

Material and Methods: The sample consisted of 407 panoramic radiographs Indonesian people who have known chronological age (8-25 years). Statistical analysis using Pearson correlation test. Regression analysis was performed to obtain the regression formula for the calculation of the age estimation.

Results: The results of the Pearson correlation test, the correlation between each of the third molars with a very strong age (> 0.75) and (P <0.05). Regression models obtained relationship summation four third molars, three molars three, two and one third molars third molars.

Conclusion: there is a very strong correlation between chronological age with the development of third molars on the Indonesian people."

"Latar Belakang : Indonesia merupakan negara yang sering dilanda bencana alam, kecelakaan dan kejahatan menyebabkan korban jiwa sehingga tidak jarang ditemukan jenazah yang hanya menyisakan tulang belulangnya. Observasi sifat anatomis dan morfologis adalah metode paling popular untuk menghubungkan ras terhadap tulang belulang. Tengkorak adalah bagian tubuh yang dipelajari secara luas dan bagian tengkorak hidung serta mulut adalah bagian terbaik untuk identifikasi ras. Tujuan: Mengetahui parameter morfologi dan morfometri pada orokraniofasial untuk menentukan ras. Metode: Sampel terdiri dari 20 tengkorak yang berasal dari pemakaman Sema Wayah di Desa Trunyan, Bali dan 7 tengkorak yang berasal dari Fakultas Kedokteran Gigi Universitas Indonesia. Penelitian ini dilakukan pengukuran pada setiap tengkorak berdasarkan parameter morfologi dan morfometri. Analisis data untuk membandingkan antara kelompok Trunyan dan Bukan Trunyan menggunakan uji univariat, bivariat dan multivariat. Hasil: Kemampuan parameter morfologi yakni Inferior Nasal Aperture, Nasal Bone Contour, Inter Orbital Breadth dalam menjelaskan ras sebesar 56,8%. Nilai rata-rata morfometri untuk total probability sebesar 2,0778 dan pada kategori sebesar 8,6296 sebagai ambang batas penentuan identifikasi ras. Apabila hasil perhitungan tersebut bernilai <0,5 artinya Trunyan >0,5 artinya Bukan Trunyan. Secara keseluruhan, model ini mampu mengidentifikasi ras Trunyan dan Bukan Trunyan sebesar 81,48%.

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Background: Indonesia is a country that is often hit by natural disasters, accidents and crimes that cause fatalities, so it is not uncommon to find bodies that only leave their bones. Observation of anatomical and morphological properties is the most popular method for relating race to bones. The skull is the most widely studied body part and the nose and mouth parts of the skull are the best parts for racial identification. Objective: To know the morphological and morphometric parameters on orocraniofacial to determine race. Methods: The sample consisted of 20 skulls from the Sema Wayah cemetery in Trunyan Village, Bali and 7 skulls from the Faculty of Dentistry, University of Indonesia. In this study, measurements were made on each skull based on morphological and morphometric parameters. Data analysis to compare between the Trunyan and Non Trunyan groups used univariate, bivariate and multivariate tests. Results: The ability of morphological parameters namely Inferior Nasal Aperture, Nasal Bone Contour, Inter Orbital Breadth in explaining race is 56.8%. The morphometric average value for the total probability is 2.0778 and in the category is 8.6296 as the threshold for determining racial identification. If the result of the calculation is <0.5, it means Trunyan > 0.5, it means Not Trunyan. Overall, this model is able to identify the Trunyan and Non-Trunyan races by 81.48%."

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Latar Belakang: Celah bibir dan palatum merupakan kelainan kongenital yang paling sering terjadi pada regio orofasial. Pasien celah bibir dan palatum menunjukkan sejumlah permasalahan seperti defek tulang alveolar yang lebar, kehilangan gigi kongenital, supernumerary teeth, hipoplasia dan gigi impaks. Autologous alveolar bone grafting dianggap sebagai perawatan gold standard untuk rekonstruksi tulang alveolar dengan menggunakan tulang kanselus dari puncak ilium anterior. Namun, pengambilan tulang ilium bersifat invasif dan memiliki potensi terjadinya komplikasi. Mengingat hal tersebut, teknik rekayasa jaringan yang memanfaatkan scaffolds, faktor pertumbuhan, dan sel punca dipertimbangkan sebagai pilihan perawatan yang baru. Sel punca mesenkim bisa didapatkan dari jaringan pulpa, yang disebut dengan sel punca pulpa gigi sulung atau stem cells from exfoliated deciduous teeth (SHED) dan sel punca pulpa gigi permanen atau dental pulp stem cells (DPSC). Salah satu kriteria yang harus dimiliki sel punca mesenkim adalah mengekspresikan surface marker CD73, CD90, dan CD105. Pada pasien normal, penelitian yang membandingkan karakteristik antara SHED dan DPSC telah membuktikan bahwa keduanya merupakan sel punca mesenkim dengan mengekspresikan surface markersesuai dengan kriteria. Namun, pada pasien celah bibir dan palatum belum banyak diteliti. Tujuan: Menganalisis perbedaan persentase sel yang mengekspresikan surface marker (CD73, CD90, dan CD105) pada SHED dan DPSC pasien celah bibir dan palatum. Metode: Sel punca pulpa gigi sulung dan gigi permanen diisolasi dari jaringan pulpa pasien celah bibir dan palatum. Persentase sel yang mengekspresikan surface marker (CD73, CD90, dan CD105) dianalisis dengan uji flow cytometry. Hasil: Analisis flow cytometry menunjukkan bahwa baik SHED maupun DPSC mengekspresikan masing-masing surface marker dalam persentase yang tinggi (>90%). Setelah dilakukan uji Independent T-test untuk membandingkan ekspresi masing-masing surface markerpada kedua grup, didapatkan hasil >0,05. Kesimpulan:  Tidak terdapat perbedaan bermakna antara ekspresi masing-masing surface marker pada SHED dan DPSC pasien celah bibir dan palatum.

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Background: Cleft lip and palate is the most common congenital anomaly in the orofacial region. Cleft lip and palate patients present with a number of complaints such as wide alveolar bone defects, congenitally missing teeth, supernumerary teeth, hypoplastic dan impacted teeth. Autologous bone grafting is considered to be the gold standard for alveolar bone reconstruction using the cancellous bone harvested from the anterior iliac crest. However, the procedure is invasive and carries a risk of complications. Bearing all that in mind, tissue engineering that utilizes scaffolds, growth factors, and stem cells arises as a new therapeutic option. Mesenchymal stem cells can be obtained from dental pulp, which are called stem cells from exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSC). One of the criterias to define mesenchymal stem cells is the expression of surface markers CD73, CD90, and CD105. In normal patients, both SHED and DPSC have been known to express those surface markers. However, the expression of CD73, CD90, and CD105 in SHED and DPSC from cleft lip and palate patients has not been fully explored. Objective: To analyze the difference in the percentage of cells that express CD73, CD90, and CD105 in SHED and DPSC from cleft lip and palate patients. Methods: SHED and DPSC were isolated from dental pulp. The expression of surface markers were analyzed with flow cytometry. Results: Flow cytometry analysis showed that SHED and DPSC from cleft lip and palate patients highly express (>90%) surface markers that are associated with mesenchymal stem cells such as CD73, CD90, and CD105. The Independent T-Test was then performed to see a comparison between the expression of each surface marker in both groups and the value was >0.05. Conclusions: There is no significant difference between the percentage of cells that express each surface marker in SHED and DPSC from cleft lip and palate patients.

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"Latar belakang: Pembentukan biofilm dan sekresi enzim hidrolase merupakan faktor virulensi Candida albicans. Salah satu enzim hidrolase yang disekresikan adalah enzim proteinase yang banyak diekspresikan pada biofilm matur Candida albicans. Temulawak yang merupakan tanaman berkhasiat obat khas Indonesia yang diketahui mampu menghambat pertumbuhan biofilm dan aktivitas enzim fosfolipase pada Candida albicans planktonik.Tujuan: Melihat aktivitas enzim proteinase pada biofilm Candida albicans yang telah terinhibisi ekstrak etanol temulawak.Metode: Pemaparan ekstrak etanol temulawak pada biofilm Candida albicans yang telah diinkubasi 90 menit, lalu diinkubasi lebih lanjut hingga mencapai fase awal (6 jam), menengah (24 jam), dan maturasi (48 jam). Biofilm yang telah terinhibisi dipindahkan pada media uji berupa bovine serum albumin agar (BSAA). Aktivitas enzim proteinase dianalisis dengan mengukur zona proteolysis yang terbentuk di luar zona koloni Candida albicans pada BSAA.Hasil: Fase awal biofilm Candida albicans yang telah terinhibisi ekstrak etanol temulawak tidak terjadi aktivitas enzim proteinase, sementara pada fase menengah dan maturasi terlihat ada aktivitas enzim proteinase tetapi tidak setinggi aktivitas enzim proteinase pada kontrol negatif.Kesimpulan: Terjadi penurunan aktivitas enzim proteinase pada berbagai fase pertumbuhan biofilm Candida albicans Candida albicans yang telah terinhibisi oleh ekstrak etanol temulawak.

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Background: Biofilm formation and hydrolase enzyme secretion are the virulence factor of Candida albicans. One of the hydrolase enzyme is secreted aspartyl proteinase or proteinase enzyme. This enzyme expressed more on mature biofilm of Candida albicans rather than on Candida albicans planktonic. Java turmeric is a native Indonesian plant which is known to have inhibition effect toward Candida albicans biofilm and could decrease the phospholipase enzyme activity of planktonic Candida albicans.Objective: To observe the activity of proteinase enzyme in Candida albicans biofilm that had been inhibited by Java turmeric ethanol extract.Method: exposure of Java turmeric ethanol extract to Candida albicans biofilm that had been incubated for 90 minutes, was followed by further incubation to reach early phase (6 hours), intermediate phase (24 hours), and maturation phase (48 hours) of biofilm formation. Inhibited biofilm then moved to the solid medium containing bovine serum albumin agar (BSAA). The activity of proteinase enzyme was analyzed by measuring the proteolytic zone seen outside the zone of Candida albicans colony on the BSAA.Result: No activity of proteinase enzyme showed on early phase of biofilm formation that had been inhibited by Java turmeric ethanol extract. On intermediate and maturation phase of biofilm that had been inhibited by Java turmeric ethanol extract showed high activity on proteinase enzyme although not as high as the activity of biofilm that had not been inhibited.Conclusion: The activity of proteinase enzyme is decreased on Candida albicans biofilm that had been inhibited by Java turmeric ethanol extract."

"Latar belakang: Penyebab stunting bersifat multifaktorial, salah satu faktor risikonya adalah malnutrisi kronis akibat kurangnya asupan protein. Kurangnya asupan protein dapat menyebabkan terjadi penurunan IGF-1, yaitu salah satu faktor pertumbuhan penting dalam pembangunan sel tubuh. IGF-1 juga memiliki peran dalam perkembangan kompleks dentoalveolar, terutama pada enamel, akar gigi, dentin, ligamen periodontal, dan jaringan pulpa gigi. Perlu dianalisis apakah gangguan perkembangan kompleks dentoalveolar akibat penuruan kadar IGF-1 pada anak stunting juga mempengaruhi waktu erupsi gigi. Tujuan: Menganalisis hubungan antara kadar IGF-1 dengan waktu erupsi gigi pada anak stunting. Metode: Pencarian literatur dilakukan dengan menggunakan pedoman alur Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) pada tiga electronic database, yaitu PubMed, EBSCO, dan Scopus. Penilaian kualitas literatur dilakukan dengan menggunakan QUADAS-2. Hasil: Terdapat 5 studi yang terpilih berdasarkan kriteria inklusi dan eksklusi. Hasil temuan penelitian menunjukkan bahwa kadar IGF-1 lebih rendah pada anak stunting dibandingkan dengan kelompok anak normal. Hal ini disebabkan karena kadar IGF-1 dalam darah dipengaruhi oleh banyak faktor, di antaranya yaitu nutrisi, status gizi, dan usia. IGF-1 yang rendah pada anak stunting berpotensi menyebabkan keterlambatan waktu erupsi gigi karena mengganggu mekanisme persinyalan molekul selama erupsi gigi, seperti BMP-2, Runx-2, dan TGF-. Kesimpulan: Terdapat korelasi positif antara kadar IGF-1 yang rendah dengan erupsi gigi pada anak stunting. Ekspresi IGF-1 yang rendah dapat menyebabkan terjadinya gangguan waktu erupsi gigi karena mengganggu proses maturasi gigi.

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Background: The causes of stunting are multifactorial, one of the risk factors causing stunting is chronic malnutrition due to lack of protein intake. Lack of protein intake can cause the decrease of IGF-1 level, which is one of the important growth factor supporting the growth and development of somatic cells. Furthermore, IGF-1 also has a role in the development of the dentoalveolar complex, especially enamel, tooth roots, dentin, periodontal ligament, and dental pulp tissues. It should be clarified whether the disturbances of dentoalveolar complex development due to decreased IGF-1 level in the stunted children would also affect the eruption time of the dentition. Objective: To analyze the relationship between IGF-1 level and the timing of tooth eruption in stunted children. Methods: Literature researches were done by using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines through three electronic databases, which were PubMed, EBSCO, and Scopus. Quality assessment of bias was examined using QUADAS-2 tool. Results: There were 5 selected studies based on inclusion and exclusion criteria. The results of the study showed that IGF-1 levels were lower in stunted children compared to normal children. The influencing factors of the level of IGF-1 in the blood, are nutritional status and age. Low level of IGF-1 in stunted children has the potential to cause delays in the timing of tooth eruption, by interrupting the activity of BMP-2, Runx-2, and TGF-β. Conclusion: There is a positive correlation between low IGF-1 level and the timing of tooth eruption in stunted children. Low IGF-1 expression can cause disturbances in the timing of tooth eruption because it interferes with the dental maturity process."

"Latar Belakang: Obat antifungal sintetik dilaporkan menimbulkan reaksi gastrointestinal. Ekstrak etanol temulawak merupakan tanaman obat yang memiliki efikasi sebagai antijamur. Untuk dijadikan obat alternatif, ekstrak etanol temulawak harus biokompatibel terhadap sel inang. Tujuan: Menganalisis efek sitotoksitas ekstrak etanol temulawak terhadap sel fibroblast gingiva secara in vitro dengan live/dead staining. Metode: Sel fibroblast gingiva passage kedua dikultur sebanyak 1,4 x 104 sel/wells di atas cover glass dalam 12 wells plate. Sel diberi perlakuan dengan konsentrasi ekstrak etanol temulawak 5% dan 20% dengan waktu paparan 1 jam, 3 jam, dan 24 jam. Viabilitas dilihat dari uji live/dead staining menggunakan confocal laser scanning microscope dengan fluorescent dye SYTO9 ex/em max: 480/500nm, PI ex/em max: 490/635nm. Hasil: intensitas fluorescent semakin tinggi berbanding lurus dengan peningkatan konsentrasi ekstrak etanol temulawak. Kesimpulan: ekstrak etanol temulawak memiliki efek sitotoksik pada konsentrasi 5% dan 20% pada sel fibroblast gingiva.

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Background: Synthetic antifungal drugs are reported to cause gastrointestinal reactions. Ethanol turmeric extract is a herbal drug that has antifungal efficacy. To be used as an alternative drug, ethanol turmeric extract must be biocompatible with host cells. Objective: Analyze the cytotoxicity of ethanol turmeric extract on gingival fibroblasts in vitro with live/dead staining. Methods: The second passage gingival fibroblast cell was cultured as much as 1.4 x 104 cells / wells on the cover glass in 12 well plates. Cells were treated with ethanol turmeric extract concentrations of 5% and 20% with exposure time of 1 hour, 3 hours and 24 hours. Viability seen from live/dead staining assay using confocal laser scanning microscope with fluorescent dye SYTO9 ex/em max: 480/500nm, PI ex/em max: 490/635nm. Results: The higher fluorescent intensity is linear to increase in concentration of dilution ethanol turmeric extract. Conclusion: Ethanol turmeric extract has a cytotoxic effect at concentrations of 5% and 20% on gingival fibroblast cells."