Hasil Pencarian  ::  Simpan CSV :: Kembali

"Endometriosis merupakan penyakit inflamasi kronis pada organ reproduksi wanita dengan gejala utama nyeri pelvis kronis, dismenore, dan dispareunia yang dapat disebabkan oleh stres oksidatif akibat rendahnya kadar antioksidan, seperti vitamin C, sehingga terjadi kerusakan sel. Levonorgestrel adalah terapi hormonal yang sering digunakan untuk meredakan rasa nyeri pada endometriosis, tetapi dapat memperberat proses inflamasi, sehingga dibutuhkan suatu terapi adjuvan, seperti propolis yang mengandung antioksidan yang tinggi. Penelitian ini menggunakan desain uji klinis dengan alokasi acak dan tersamar ganda. Subjek penelitian adalah 24 wanita yang sedang mendapatkan terapi implan levonorgestrel dan diminta untuk menerima propolis atau plasebo dua kali sehari dengan dosis 1 tetes/10 kg berat badan (kgBB) per kali. Sampel darah kemudian diambil pada 4 minggu setelah intervensi dan dilakukan pemisahan serum. Pengukuran kadar vitamin C serum dilakukan dengan metode spektrofotometri dan analisis statistik dilakukan dengan uji t tidak berpasangan apabila data berdistribusi normal. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan antara kedua kelompok (p<0,001), yaitu kadar vitamin C serum lebih tinggi pada kelompok propolis (0,202+0,057) dibandingkan kelompok plasebo (0,069+0,028). Dengan demikian, pemberian propolis meningkatkan kadar vitamin C serum pada pasien endometriosis setelah intervensi 4 minggu. 

---

Endometriosis is a chronic inflammatory reproductive disease in women which main symptoms are chronic pelvic pain, dysmenorrhea, and dyspareunia that can be triggered by oxidative stress due to decreased antioxidants, such as vitamin C that may cause cell damage. Levonorgestrel is a hormonal therapy that is commonly used for endometriosis to relieve pain but it can worsen the inflammatory process, so an adjuvant therapy is needed, such as propolis that contains high antioxidant level. This study used clinical trial design with random allocation and double-blinded. The study subject is 24 women that receive levonorgestrel therapy and were asked to consume propolis or placebo randomly two times a day with a dose of 1 drop/10 kg body weight (kgBW) per time. Blood samples were then taken after 4 weeks and serum separation was performed. Serum vitamin C levels were measured using spectrophotometric method and statistical analysis used independent t-test if the data were normally distributed. The result showed that there is a significant difference between the two groups (p<0,001), in which the concentration of serum vitamin C is higher in the propolis group (0,202+0,057) compared to the placebo group (0,069+0,028). In conclusion, the administration of propolis results in significantly higher serum vitamin C concentration after 4-week intervention."

"Endometriosis merupakan penyakit ginekologi kronis yang dapat dipicu oleh stres oksidatif akibat peningkatan spesies oksigen reaktif (ROS), yang mengakibatkan ketidakseimbangan glutation sebagai antioksidan endogen dan kerusakan sel, dengan 8-Hidroksi-2-Deoksi guanosin (8-OHdG) sebagai biomarker. Levonorgestrel sebagai terapi hormonal untuk endometriosis dapat mengganggu dan mempengaruhi stres oksidatif juga. Flavonoid adalah bahan bioaktif alami seperti produk lebah, sebagai antioksidan yang dapat menekan proliferasi sel-sel patologis. Penelitian ini bertujuan untuk menentukan efek Flavonoid terhadap kadar glutation dan kondisi 8-OHdG. Penelitian ini menggunakan desain uji klinis dengan alokasi acak dan double-blinded. 24 wanita dengan terapi Levonorgestrel (LNG) karena endometriosis secara acak ditugaskan untuk menerima propolis yang mengandung 17,5 mg flavonoid per tetes atau plasebo. Intervensi diberikan dua kali sehari, pada pagi dan malam hari, dengan dosis 1 tetes/10 kg berat badan (kgBB) per kali. Sampel darah dan penilaian gizi diambil pada kunjungan pertama dan 4 minggu setelahnya. Hasil penelitian menunjukkan bahwa glutation dan 8-OHdG tidak memiliki perbedaan yang signifikan antara kedua kelompok (p>0,05), namun glutation mengalami penurunan sebesar 0,01 (-0,01-0,037) μg/mL setelah 4 minggu intervensi. Kadar 8-OHdG menunjukkan penurunan yang lebih besar pada kelompok propolis sebesar 17,30 ng/mL (-13,58 – 37,19) ng/mL dibandingkan dengan kelompok plasebo. Pemberian flavonoid dalam propolis tidak menghasilkan perubahan yang signifikan dalam kadar glutation dan 8-OHdG selama periode intervensi 4 minggu.

---

Background: Endometriosis represents a chronic gynecological disease that  can be triggered by oxidative stress due to increased reactive oxygen species (ROS) resulting in an imbalance of glutathione as an endogen antioxidants and cell damage, with 8-Hidroksi-2-Deoxy guanosine (8-OHdG) as biomarker. levonorgestrel as a hormonal therapy for endometriosis can interfere and may affect the oxidative stress either. Flavonoids are natural bioactive ingredients such as bee product, as antioxidants that may suppress proliferation of pathological cells.

Objectives: This study aimed to determine the effect of Flavonoid on glutathione level and 8-OHdG condition.

Methods: This study used clinical trial design with random allocation and double-blinded. 24 women with Levonorgestrel (LNG) therapy due to endometriosis were randomly assigned to receive propolis-contained 17.5 mg of flavonoids per drop or placebo. The intervention given two times a day,in the morning and at night, with a dose of 1 drop /10 kg body weight (kgBW) per time. Blood samples and nutritional assessment were taken at the first time of visit and 30 days thereafter.

Results: The results showed that glutathione and 8-OHdG did not have a significant difference between the two groups (p>0,05), but glutathione decreased 0,01(-0,01-0,037) μg/mL after 4 weeks of intervention. The 8-OHdG levels showed a greater decrease in the propolis group by 17,30 ng/mL (-13.58 – 37.19) ng/mL compared to the placebo group.

Conclusion: The administration of flavonoids in propolis did not result in significant changes in glutathione and 8-OHdG levels during the 4-week intervention period."

"Endometriosis merupakan penyakit ginekologi umum yang dipicu terjadinya peradangan kronis yang ditandai dengan produksi beberapa sitokin pro-inflamasi, salah satu yang terbanyak yaitu TNF-α. Di sisi lain, sitokin anti-inflamasi, seperti IL-10, dapat mengakhiri proses inflamasi berlanjut ini. Propolis adalah bahan bioaktif alami produk lebah, sebagai imunomodulator dan efek anti-inflamasi yang dapat menekan proliferasi sel-sel patologis. Penelitian ini bertujuan untuk menentukan pengaruh pemberian propolis terhadap tumor necrosis factor alpha dan interleukin 10. Penelitian ini menggunakan desain uji klinis dengan alokasi acak dan double-blinded. 24 wanita dengan terapi Levonorgestrel (LNG) karena endometriosis secara acak ditugaskan untuk menerima propolis yang mengandung 17,5 mg flavonoid per tetes atau plasebo. Intervensi diberikan dua kali sehari, pada pagi dan malam hari, dengan dosis 1 tetes/10 kg berat badan (kgBB) per kali. Sampel darah dan penilaian gizi diambil pada kunjungan pertama dan 30 hari setelahnya. Hasil penelitian menunjukkan bahwa kadar tumor necrosis factor alpha dan interleukin 10 tidak memiliki perbedaan yang signifikan antara kedua kelompok (p>0,05). Kadar tumor necrosis factor alpha mengalami penurunan yang lebih besar pada kelompok propolis sebesar 4,17 (44,36-50,05) pg/mL dibandingkan dengan kelompok plasebo. Kadar IL-10 menunjukkan peningkatan sebesar 344,94 setelah 30 hari diberikan intervensi. Pemberian flavonoid dalam propolis tidak menghasilkan perubahan yang signifikan dalam kadar Tumor Necrosis Factor Αlpha dan Interleukin 10 selama periode intervensi 30 hari.

---

Endometriosis is a common gynecological disease triggered by chronic inflammation characterized by the production of several pro-inflammatory cytokines, one of which is TNF-α. On the other hand, anti-inflammatory cytokines, such as IL-10, can end this ongoing inflammatory process. Propolis are natural bioactive ingredients contained in bee products, as immunomodulators and anti-inflammatory effects that can suppress the proliferation of pathological cells. This study aimed to determine the effect of propolis supplementation on tumor necrosis factor alpha and interleukin 10. This study used clinical trial design with random allocation and double- blinded. 24 women with Levonorgestrel (LNG) therapy due to endometriosis were randomly assigned to receive propolis-contained 17.5 mg of flavonoids per drop or placebo. The intervention given two times a day,in the morning and at night, with a dose of 1 drop /10 kg body weight (kgBW) per time. Blood samples and nutritional assessment were taken at the first time of visit and 30 days thereafter. The results showed that the levels of tumor necrosis factor alpha and interleukin 10 did not differ significantly between the two groups (p>0.05). Tumor necrosis factor alpha levels experienced a greater decrease in the propolis group by 4.17 (44.36-50.05) pg/mL compared to the placebo group. IL-10 levels showed an increase of 344.94 after 30 days of intervention. The administration of propolis supplementation did not result in significant changes in the levels of Tumor Necrosis Factor Αlpha and Interleukin 10 during the 30- day intervention period."

"Pendahuluan: Endometriosis berkaitan dengan adanya inflamasi  kronik,  gangguan  maturasi  oosit,  peningkatan stress  oksidatif  radikal,  dan  apoptosis  yang  kemudian  mendasari  terjadinya  infertilitas pada wanita usia subur.  Fertilisasi In Vitro (FIV)  merupakan  salah  satu  teknik  yang dapat menangani  infertilitas  dengan  tingkat  keberhasilannya  bergantung  dengan  kualitas  oosit  yang diambil  untuk  menjadi  embrio.  Kualitas oosit dipengaruhi  oleh  nutrisi. Profil  mikronutrien  seperti  vitamin  D  dan  zink  dianggap mempengaruhi  fungsi  reproduksi  melalui aktivitas  anti-inflamasi,  anti-apoptosis dan  anti-oksidan  yang dimiliki. Sayangnya, urgensi untuk menjaga adekuasi nutrisi  ini  sering diabaikan. Selain itu juga hingga saat ini  belum  ada  acuan  untuk  memprediksi  kadar  profil  vitamin  D  dan  zink  dalam serum  dan  cairan  folikular  yang  berhubungan  pada  pasien  endometriosis  itu  sendiri.Tujuan: Penelitian ini membandingkan kadar vitamin D dan zink di serum dan cairan folikular pada pasien endometriosis dan non-endometriosis.Metode: Studi ini merupakan studi potong lintang yang dilaksanakan pada pasien Fertilisasi In Vitro (FIV) di Klinik Yasmin, RSCM Kencana selama Juli –  Desember 2020. Data klinis diperoleh melalui rekam medis dan wawancara pasien. Data laboratorium diperoleh melalui sampel darah dan cairan folikuler yang diperoleh bersamaan dengan prosedur Ovum Pick Up (OPU). Sampel kemudian dikelompokkan menjadi kelompok endometriosis dan non-endometriosis. Setelah itu data disajikan dalam tabel dan dianalisis dengan uji parametrik, yaitu uji-t berpasangan bila sebaran data normal atau uji non parametrik, yaitu uji Mann-Whitney bila sebaran data tidak normal. Data dianalisis dengan SPSS 24.Hasil: Dari jumlah sampel 26 pasien pada studi ini, didapatkan tidak adanya perbedaan bermakna dari vitamin D serum pada pasien endometriosis (22,83 (5,00 – 40,00)) dan non endometriosis (30,11 (10,40-76,10)), namun secara rerata kadar vitamin D serum pada pasien endometriosis lebih rendah. Kadar vitamin D cairan folikular pada pasien endometriosis (15,33 (4,50-36,32)) dan non-endometriosis (23,64 (4,98-60,22)) tidak berbeda bermakna (P>0,05). Kadar zink serum pada pasien endometriosis (75,23 ± 11,58) dan non-endometriosis (79,46 ± 12,09) tidak berbeda bermakna (P>0,05). Konsentrasi zink paada cairan folikular tidak menunjukkan perbedaan bermakna antara pasien endometriosis (39,00 (22,00 – 49,00)) dan tanpa endometriosis (51,00 (19,00-95,00)) (P>0,05). Kesimpulan: Tidak ditemukan perbedaan bermakna antara kadar vitamin D serta zink serum dan cairan folikular pada pasien endometriosis dan non-endometriosis. 

---

Background: Endometriosis associates with chronic inflammation, dysfunction of oocyte maturation, increase of oxidative stress, and apoptosis which underlie infertility problem in reproductive female. In-Vitro Fertilization (IVF) is a technique used to treat infertility with the success rate depending on the quality of the oocytes extracted to become embryos. Oocyte quality is influenced by nutrition. Micronutrient profiles such as vitamin D and zink are thought to influence reproductive function through their anti-inflammatory, anti-apoptotic and anti-oxidant activities. However, the urgency of maintaining nutritional adequacy is often overlooked. In addition, until now there is no reference for predicting levels of vitamin D and zink in serum and follicular fluid associated with endometriosis.

Method: This study is a cross-sectional study conducted on In Vitro Fertilization (FIV) patients at the Yasmin Clinic, RSCM Kencana during July – December 2020. Clinical data were obtained through medical records and patient interviews. Laboratory data were obtained through blood and follicular fluid samples obtained in conjunction with the Ovum Pick Up (OPU) procedure. The samples were then grouped into endometriosis and non-endometriosis groups. After that the data are presented in tables and analyzed by parametric test, namely paired t-test if the data distribution is normal or non-parametric test, namely the Mann-Whitney test if the data distribution is not normal. Data were analyzed by SPSS 24.

Result: From a total sample of 26 patients in this study, there was no significant difference in serum vitamin D in patients with endometriosis (22.83 (5.00 – 40.00)) and non-endometriosis (30.11 (10.40-76.10). )), but the mean serum vitamin D level in endometriosis patients was lower. Follicular fluid vitamin D levels in patients with endometriosis (15.33 (4.50-36.32)) and non-endometriosis (23.64 (4.98-60.22)) were not significantly different (P>0.05) . Serum zink levels in patients with endometriosis (75.23 ± 11.58) and non-endometriosis (79.46 ± 12.09) were not significantly different (P>0.05). Zink concentration in follicular fluid did not show a significant difference between endometriosis patients (39.00 (22.00 – 49.00)) and without endometriosis (51.00 (19.00-95.00)) (P>0.05) .

Conclusion: There was no significant difference between the levels of vitamin D and serum zink and follicular fluid in patients with endometriosis and non-endometriosis."

"ABSTRACT

Objective : To study the eH`ect of vitamin C 1000 mg i.v and E 400 mg oral supplementation on serum c-reactive protein level as parameter of inflammation in burn patients.

Methods: This study was a one group pre post test that gave i.v 1000 mg vitamin C and oral 400 mg vitamin E supplementations to thirteen moderate-severe burn patients, with percentage of burn less than 60%, in burn unit Cipto Mangunkusumo Hospital. Data were collected using questionnaire, medical record, anthropometric measurement, dietary assessment using four consecutive days food record. Laboratory test for serum vitamin C, E and serum c-reactive protein levels- were evaluated before and after supplementations. Differences in mean values were assessed by Wilcoxon for the not normal distribution.

Results: Among thirteen subjects, Seven (53.80%) Subjects were female, median of age 35 (18-55) years. Body mass index in most subjects (69.2%) were categorized as normal. The median percentages of burn injury 22 (5~57)%, and the frequency of severe burn was 6l.50%, while the most cause of burn was flame (76.9%). Level of vitamin C after treatment was increased, but not significant. Level of vitamin E after treatment was significantly increased (p=0,016). Level of CRP after supplementation significantly increased (p=0.04).

Conclussion: There was significantly reduced of level serum CRP after four days vitamin C1000 mg i.v dan E 400 mg oral supplementations."

"Abstract

The Effect of Vitamin C and E Suplementations on Serum CReaetive Protein Level in Moderate-Severe Bum Patients.

To study the effect of vitamin C 1000 mg i.v and E 400 mg oral supplementation on serum c-reactive protein level as parameter of inflammation in bum patients.

This study was a one group pre post test that gave i. v 1000 mg vitamin C and ond 400 mg vitamin E supplementations to thirteen moderate-severe bum patients, with percentage of bum less than 60%, in bum unit Cipto Mangunkusumo Hospital. Data were collected using questionnaire, medical record, anthropometric measurement, dietary assessment using fol.lf consecutive days food record. Laboratory test for serum vitamin C, E and serum c-reactivc protein levels were evaluated before and after supplementations. Differences in mean values were assessed by Wilcoxon for the not normal distribution.

Results Among thirteen subjects, seven (53.80%) subjects were female, median of age 35 (18-55) years. Body mass index in most subjects (69.2%) were categorized as normal. The median percentages of bum injury 22 (5-57}%, and the frequency of severe bum was 61.50%, while the most cause of bum was flame (76.9%). Level of vitamin C after treatment was increased, but not significant. Level of vitamin E after treatment was significantly increased (!Pi),OI6). Level of CRP after supplementation significantly increased (!Pi).04).

Conclusion There was significantly reduced of level serum CRP after four days vitamin CI 000 mg i. v dan E 400 mg ond supplementations."

"[Vitamin A berperan dalam diferensiasi sel sehingga memodulasi diferensiasi sel TH2 menjadi IL-4.Infeksi A.lumbricoides merupakan suatu penyakit yang menginduksi dominansi respons imun sel TH2.Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian vitamin A pada ibu hamil terinfeksi A.lumbricoidesterhadap sitokin IL-4.Penelitian ini menggunakan desain eksperimental analitik berdasarkan data sekunder.Total sampel yang digunakan adalah 39 sampel, terbagi menjadi dua kelompok, yaitu plasebo (21) dan vitamin A (18). Pemeriksaan telur per gram tinja (TPG) dilakukan dengan metode Kato-Katz, sedangkan konsentrasi IL-4 serum diperiksa dengan metode ELISA.Pemeriksaan tersebut dilakukan sebelum dan setelah intervensi.Sebelum intervensi, rerata konsentrasi IL-4 pada kelompok vitamin A 70,7 pg/mL, sedangkan kelompok plasebo 60,6 pg/mL.Setelah intervensi didapatkan perubahan konsentrasi IL-4 yang bernilai positif(p=0,000) pada kelompok vitamin A (53,98 pg/mL) dan plasebo (99,55 pg/mL). 16 dari 18 subjek penelitian di kelompok vitamin A mengalami peningkatan konsentrasi IL-4.Hal tersebut disertai penurunan telur per gram tinja (TPG) pada 17dari 18 subjek, namun secara statistik tidak bermakna (p=1,000).Pada kelompok plasebo, seluruh subjek penelitian mengalami peningkatan konsentrasi IL-4, akan tetapipenurunan TPG hanya terjadi pada 4 dari 21 subjek (p=1,000). Kesimpulan pada penelitian ini yaitupemberian vitamin A secara bermakna mempengaruhi perubahan konsentrasi sitokin IL-4 yang bernilai positif sehingga berdampak terhadap penurunan TPG A.lumbricoides pada ibu hamil terinfeksi., Vitamin A plays a role in cells differentiation so that it modulates TH2 differentiation into IL-4. A.lumbricoides infection generates a modified TH2 immune response during its course. The aim of this study is to know the effect of vitamin A supplementation among A.lumbricoides infected pregnant women on IL-4 serum concentration. This is a experimentalstudy based on a secondary data. Total sample used is 39 which divided into two groups, placebo(21) and vitamin A(18). Egg per gram (EPG) of feces is measured by using Kato-Katz method before and after intervention. ELISA is used to measure IL-4 serum concentration. After intervention, there was a significant differentiation (p=0,000) between the alteration of IL-4 serum concentration in vitamin A (mean=53,98 pg/mL) and placebo (mean=99,55 pg/mL) group. 16 of 18 subjects in vitamin A group had an increasing level of IL-4 serum concentration, followed by the reduction of EPG (p=1.000) in 17 of 18 subjects. On the other hand, IL-4 serum concentration increased in all subjects in placebo group but the reduction of EPG only happened in 4 of 21 subjects (p=1.000). The conclusion of this study is vitamin A supplementation significantly affect the alteration of IL-4 serum concentration. Besides, IL-4 may play a role to decrease EPG in A.lumbricoides infected pregnant women.]"

"Serat asbes yang terinhalasi masuk ke dalam alveolus menyebabkan terjadinya peningkatan produksi reactive oxigen spesies (ROS) yang dapat memicu terjadinya reaksi inflamasi. Interleukin 6 merupakan penanda reaksi inflamasi akibat pajanan serat asbes. Vitamin C dan E merupakan antioksidan yang bekerja sebagai scavenger ROS. Vitamin C juga dapat menghambat aktivitas faktor transkripsi NFқB. Vitamin E selain dapat menghambat aktivitas faktor transkripsi JAK/STAT3 dan NFқB, juga dapat menghambat aktivitas COX2 dan LOX5. Penelitian potong lintang di sekretariat serikat buruh pabrik asbes X Kabupaten Karawang bulan Oktober 2014 dilakukan untuk menilai korelasi asupan vitamin C, E dengan kadar interleukin 6 pada pekerja pabrik asbes. Lima puluh dua pekerja pabrik asbes berhasil menyelesaikan protokol penelitian. Hasilnya menunjukkan tidak terdapat korelasi bermakna (p >0,05) antara asupan vitamin C dengan kadar IL-6 dan antara asupan vitamin E dengan kadar IL-6. Terdapat korelasi positif antara kadar vitamin C dengan kadar IL-6 (r = 0,31) dengan p <0,05, namun tidak terdapat korelasi antara kadar vitamin E dengan kadar IL-6.

---

Asbestos fibers that are inhaled into the alveoli cause increased production of reactive oxygen species (ROS) which may trigger inflammation reaction. Interleukin 6 (IL-6) is a marker of inflammation reaction caused by asbestos fibers exposure. Vitamin C and vitamin E are antioxidants acting as ROS scavengers. Vitamin C can also inhibit the activity of transcription factor NFқB. Vitamin E can inhibit the activities of transcription factors JAK/STAT3 and NFқB as well as the activities of COX2 and LOX5.

A cross-sectional sudy at a labor union secretariat in Karawang Regency in October 2014 was conducted to evaluate the correlations between intakes and levels of vitamin C and vitamin E and level of IL-6 in asbestos factory workers. Fifty two asbestos factory workers finished the study. The result showed no significant correlation between vitamin C intake and IL-6 level or between vitamin E intake and IL-6 level. There was a moderate positive correlation between vitamin C level and IL-6 level (r = 0.31, p <0.05), but there was no correlation between vitamin E level and IL-6 level."

"Mengetahui pengaruh suplementasi vitamin C 1000 mg i.v dan E 400 mg oral selama empat hari berturut-turut terhadap kadar malondialdehid (MDA) plasma, sebagai penanda stres oksidatif pada penderita luka bakar sedang berat. Penelitian ini merupakan one group pre post test, yang memberikan suplementasi vitamin C 1000 mg i.v dan vitamin E 400 mg oral yang pada 13 subyek penclitian, yaitu penderita luka bakar kategori sedang berat dengan tuas luka bakar kurang dari 60%, yang dirawat di Unit Luka Bakar RSUPN Cipto Mangunkusumo. Data diperoleh melalui wawancara, rekam medik, pengukuran antropometri, analisis asupan menggunakan metodefood record, dan pemeriksaan laboratorium. berupa pemeriksaan kadar vitamin Ct E serum dan MDA plasma pada sebeium dan setelah suplementasi. Analisis data untuk data berpasangan menggunakan uji t berpasangan dan uji Wileoxon, sedangkan untuk dua kelompok tidak berpasangan menggunakan uji Mann Whitney. Batas kemaknaan pada penelitian ini adalah 5%. Sebanyak 13 orang subyek penelitian~ terdiri dari perempuan 53,85%, dengan median usia 32 (18-55) tahun, sebagian besar subyek memiliki status gizi normal (61.54%). Median luas luka bakar adalah 22 (5-57)"/o, dengan kasus terbanyak adalah luka bakar berat (61.50%), dan penyebab terbanyak adalah api (76.9%). Kadar vitamin C pasca suplementasi mengalami sedikit peningkatan yang tidak bennakna. Kadar vitamin E subyek penelitian meningkat bermakna (p=O,Ol6) pasea suplementasi, walaupun masih dalam kategori rendah. Kadar MDA pada suplementasi mengalami penurunan bermakna W'(l,O 19). Simpulan: Terdapat penurunan bermakna kadar MDA plasma pada subyek penelitian setelah suplementasi vitamin C 1000 mg i.v dan vitamin E 400 mg oral selama empat hari.

---

To study the effect of vitamin C 1000 mg i.v and E 400 mg oral supplementation on plasma malondialdehyde level as parameter of oxidative stress in bum patients.

This study was a one group pre post test that gave i.v 1000 mg vitamin C and omi 400 mg vitamin E supplementations to thirteen moderate-severe bum patient$. with percentage of bum less than 60%, in burn unit Cipto Mangunkusurno HospitaL Data were collected using questionnaire, medical record, anthropometric measurement. dietary assessment using four consecutive days food record. Laboratory test for serum vitamin C, B and plasma malondialdehyde levels were evaluated before and after supplementations. Differences in mean values were assessed by paired t-test for normal distribution data or Wilcoxon fur the not normal distribution. Mann Whitney was used for unpaired data. Values of p < O~OS were considered to indicate statistical significance.

Results Among thirteen subjects. seven (53.80%) subjects were female, median of age 32 (18-55) years. Body mass indek in most subjects (61.54%) were categorized as normal. The median percentages of bum injury 22 (5-51)%, and the frequency of severe bum was 61.50%, while the most cause of bum was flame (76,9%). Level of vitamin C after treatment was increased, but not significant. Level of vitamin E after treatment was significantly increased (p=O,Ol6). Level of MDA after supplementation significantly reduced (Jl 0,0l9).

Conclusion There was significantly reduced of level plasma MDA after four days vitamin CIOOO mg i.v danE 400 mg oral supplementations. "

"Tujuan penelitian adalah untuk mengetahui korelasi antara kadar vitamin C serumdengan kadar SOD eritrosit pada penderita HIV/AIDS . Penelitian dilakukan di UPTHIV RSUPNCM Jakarta mulai bulan Februari sampai Maret 2013. Penelitian inimerupakan studi potong lintang terhadap 52 orang penderita HIV. Data yang diambilmeliputi data karakteristik subyek berdasarkan usia, jenis kelamin dan pendidikan,asupan energi, asupan vitamin C, status gizi, riwayat pengobatan ARV, jumlahlimfosit T CD4. Dilakukan pemeriksaan laboratorium untuk mengukur kadar vitaminC serum dan kadar SOD eritrosit. Analisis korelasi menggunakan uji Pearson dengankemaknaan p<0,05. Hasil: Subyek penelitian 25 perempuan dan 27 laki-laki, reratausia 33,60±4,84 tahun. 80,8% berada dalam rentang usia 30–40 tahun dan 82,7%berpendidikan sedang. Asupan energi 76,9% kurang dengan rerata untuk perempuan1700,41±316,25kkal/hari dan rerata laki-laki 1996,33±525,72kkal/hari. Asupanvitamin C 100% kurang dengan rerata untuk perempuan 46,62±15,66mg/hari danlaki-laki 46,97±13,39mg/hari. Status gizi 44,2% cukup dan 40,4% lebih dengan rerataIMT 21,98±3,48kg/m2. Sebanyak 94,2% sudah mendapat ARV dan jumlah limfosit TCD4 terbanyak berada pada kategori II CDC (200–499sel/?L) yaitu sebanyak 63,5%dengan median 245(50–861)sel/?L. Kadar vitamin C serum sebanyak 92,3% dalamkategori rendah dengan median 0,23(0,10–0,56)mg/dL. Kadar SOD eritrositterbanyak (53,8%) dalam kategori normal dengan rerata 1542,10±5,42U/gHb.Terdapat korelasi negatif lemah yang tidak bermakna antara kadar vitamin C serumdengan kadar SOD eritosit (r= −0,109 dan p=0,442)

---

The objective of this study was to investigate the correlation between serum vitamin

C concentration and erythrocyte SOD concentration of HIV/AIDS patients. Study

was conducted at UPT HIV/AIDS RSUPNCM from February to March 2013. The

study was a cross sectional study of 52 HIV/AIDS patients. Data collected including

subject characteristic age, sex, education, energy intake by food record 2x24 hour,

vitamin C intake by FFQ semikuantitatif, nutritional status, history of ART, and CD4

lymphocyte count. Conducted laboratory tests to measure serum vitamin C

concentration and erythrocyte SOD concentration. Statistical analysis was done using

Pearson’s correlation test.

Result: Subject consisted of 27 men and 25 women, mean of age 33.60±4.84years

old. 80.8% age in range 30–40years old. 82.7% were medium education level. 76.9%

subject had low energy intake, mean 1700.41±316.25kcal/day for women and mean

1996.33±525.72kcal/day for men. 100% subject had low vitamin C intake with mean

46.62±15.66mg/day for women and 46.97±13.39mg/day for men. . Nutritional status

of 44.2% had normal and 40.4% over enough with a mean BMI 21.98±3.48 kg/m2.

94.2% had ART and 63.5% lymphocyte count at category II CDC with mean

245(50–861)cell/?L. 92.3% subyek had low serum vitamin C concentration with

median 0.23(0.10–0.56)mg/dL. 53.8% subject had normal erythrocyte SOD

concentration with mean 1542.10±5.42U/gHb. There was no correlation between

serum vitamin C and erythrocyte SOD. (r=−0.109 and p=0.442)"