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"Asam lemak merupakan salah satu produk yang cukup aplikatif di industri. Produk ini umum didapatkan dengan bahan baku minyak nabati. Metode produksi asam lemak tersebut melibatkan ekstraksi minyak dari BSFL (black soldier fly larvae), yang dapat dilakukan melalui berbagai metode, khususnya ekstraksi cair dengan pelarut organik dan ekstraksi mekanis menggunakan screw pressing. Proses simulasi ini menggunakan perangkat lunak SuperPro Designer v.13 dan AspenPlus v.12.1. BSFL yang telah dibudidayakan akan dijadikan sebagai umpan proses sebesar 10 ton/batch. Produksi asam lemak dan produk samping lainnya berupa produk padatan dan gliserin. Proses dimulai dengan pretreatment dan proses ekstraksi minyak, baik dengan pelarut cair atau screw press. Ekstrak berupa minyak BSFL kemudian akan di-splitting sehingga jumlah asam lemak bebasnya maksimal untuk difraksinasi dengan distilasi. Proses ini bernilai ekonomis dengan mempertimbangkan gate fee sebesar $57,6 USD/ton untuk pengolahan limbah oleh BSFL sebagai salah satu penghasilannya. Adapun dari proses dengan ekstraksi pelarut cair, didapatkan nilai NPV (net present value) sebesar  $1.281.065 USD, IRR (internal rate of return) 11,4%, ROI (rate of investment) 5,4%, dan PBP (payback period) 7,5 tahun. Sedangkan untuk ekstraksi dengan screw press, NPV sebesar  $3.584.990 USD, IRR 17,5%, ROI 10,7% dan PBP 5,5 tahun. Berdasarkan analisis sensitivitas, gate fee menjadi variabel yang relatif sensitif pada kedua jenis proses.

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Fatty acids are one of the quite applicable products in the industry. This product is commonly obtained from vegetable oil raw materials. The production method of these fatty acids involves extracting oil from BSFL (black soldier fly larvae), which can be done through various methods, particularly liquid extraction with organic solvents and mechanical extraction using screw pressing. This simulation process uses SuperPro Designer v.13 and AspenPlus v.12.1 software. Cultivated BSFL will be used as the process feed at a rate of 10 tons/batch. The production includes fatty acids and other by-products such as solid products and glycerin. The process starts with pretreatment and oil extraction, either with liquid solvent or screw press. The extracted BSFL oil will then be split so that the amount of free fatty acids is maximized for fractionation by distillation. This process is economically valuable, considering the gate fee of $56,7 USD for waste processing by BSFL as one of its revenue sources. From the liquid solvent extraction process, a NPV (net present value) of $1.281.065 USD, IRR (internal rate of return) of 11,4%, ROI (rate of investment) of 5,4%, and PBP (payback period) of 7,5 years were obtained. Meanwhile, a NPV of screw press extraction is $3.584.990 USD, IRR of 17,5%, ROI of 10,7%, and PBP of 5,5 years were obtained. Based on sensitivity analysis, the gate fee is a relatively sensitive variable in both types of processes."

"One main drawback of the local animal industry is the inavailability of affordable and sustainable protein supply for the livestock. Insect larvae, such as the Black Soldier Fly (Hermetia illucens) larvae (BSFL), have been considered as a protein source which can be produced at a large scale using low cost organic wastes as feeding material. This study was designed to determine the response of BSFL to various waste combinations of vegetable and animal remains, Pak Choi (Brassica chinensis) residues (S) and carp (Cyprinus caprio) fish offal (I)). A total of 540 BSFL were fed with 100 mg/larvae/day combination of vegetable wastes: animal waste 70%: 30% (S > I), 50%: 50% (S = I), and 30%: 70% (S < I). Among the feed combinations, the S < I group showed the best results as it produced the significantly highest weight of BSFL at 122.8 mg/larvae and approximate digestibility of 62.01%, with the least pupae mortality rate at 4.29%.Keywords: bioconversion, biomass, Brassica chinensis, Cyprinus carpio, Hermetia illucen"

"One main drawback of the local animal industry is the inavailability of affordable and sustainable protein supply for the livestock. Insect larvae, such as the Black Soldier Fly (Hermetia illucens) larvae (BSFL), have been considered as a protein source which can be produced at a large scale using low cost organic wastes as feeding material. This study was designed to determine the response of BSFL to various waste combinations of vegetable and animal remains, Pak Choi (Brassica chinensis) residues (S) and carp (Cyprinus caprio) fish offal (I)). A total of 540 BSFL were fed with 100 mg/larvae/day combination of vegetable wastes: animal waste 70%: 30% (S > I), 50%: 50% (S = I), and 30%: 70% (S < I). Among the feed combinations, the S < I group showed the best results as it produced the significantly highest weight of BSFL at 122.8 mg/larvae and approximate digestibility of 62.01%, with the least pupae mortality rate at 4.29%."

"Limbah industri kelapa sawit Palm Kernel Meal (PKM) dan sampah organik mengandung bahan organik yang tinggi namun belum dimanfaatkan dengan optimal. Pengelolaan sampah yang baik harus dilakukan dengan daur ulang sampah organic. Oleh karena itu, diperlukan solusi berupa biokonversi menggunakan larva Black Soldier Fly (Hermetia illucens) dengan penambahan perlakuan fermentasi untuk mengoptimalkan proses biokonversi. Penelitian ini bertujuan untuk menganalisis pengaruh fermentasi PKM dan sampah organik terhadap pertumbuhan larva Black Soldier Fly (BSF); dan kandungan nutrisi larva BSF. Larva BSF berusia 6 hari (6-DOL) dipelihara pada media pertumbuhan dengan lima perlakuan berbeda, yaitu campuran limbah PKM dan sampah organik fermentasi dan tanpa fermentasi, Analisis data yang digunakan adalah uji normalitas Shapiro-Wilk dan uji homogenitas Levene’s test, dilanjutkan dengan uji ANOVA dengan derajat kepercayaan α=0,05. Hasil penelitian diperoleh campuran limbah dengan fermentasi efektif digunakan sebagai media pertumbuhan larva BSF dalam meningkatkan kelangsungan hidup, konsumsi pakan, indeks reduksi limbah, dan kandungan nutrien berupa protein dan lemak yang tinggi. Sedangkan campuran limbah tanpa fermentasi efektif dalam meningkatkan biomassa larva dan efisiensi konversi pakan. Hasil analisis data menunjukkan penambahan perlakuan fermentasi PKM dan sampah organik sebagai media pertumbuhan tidak berpengaruh signifikan terhadap pertumbuhan larva BSF. Penambahan fermentasi PKM dan sampah organik berpengaruh terhadap kandungan nutrisi larva BSF.

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Palm oil industrial waste Palm Kernel Meal (PKM) and organic waste contain high organic matter but have not been utilized optimally. Good waste management must be done by recycling biowaste. Therefore, we need a solution, namely by bioconversion using Black Soldier Fly (Hermetia illucens) larvae with the addition of fermentation treatment to optimize this bioconversion process. This research aims to analyze the effect of PKM and organic waste fermentation on the growth of Black Soldier Fly (BSF) larvae and nutritional content of BSF larvae. BSF larvae aged 6 days (6-DOL) were maintained on the feed media with five different treatments, namely a mixture of PKM and organic waste fermented and unfermented. Data analysis used was the Shapiro-Wilk test of normality and the Levene's test of homogeneity, continued with ANOVA test with a degree of confidence α =0.05. The results of research showed fermented waste mixture was effectively used as a growth media for BSF larvae in increasing survival rate of BSF larvae, feed consumption rate, waste reduction index, and high nutrient content namely protein and lipid. While the unfermented waste mixture was effectively used as a growth medium for BSF larvae in increasing the biomass of BSF larvae and efficiency conversion of ingested food. The results of data analysis showed that the addition of PKM and organic waste fermentation treatment as a growth medium had no significant effect on the growth of BSF larvae. The addition of PKM and organic waste fermentation affected the nutritional content of BSF larvae."

"Sampah sisa makanan menjadi salah satu masalah di Universitas Indonesia karena belum terdapat metode yang efektif dan efisien dalam pengelolaan limbah kantin dari fakultas-fakultas yang ada. Salah satu teknik pengelolaan limbah kantin adalah dengan pengomposan menggunakan larva Black Soldier Fly (BSF). Adanya proses fermentasi dan penguraian bahan kompleks oleh mikroorganisme pada substrat organik mampu mengoptimalkan daya biokonversi larva BSF. Namun, hingga saat ini, penelitian terkait pengaruh fermentasi limbah kantin terhadap efektivitas biokonversinya dengan larva BSF belum ditemukan sehingga penelitian ini perlu dilakukan. Penelitian ini bertujuan untuk mengidentifikasi pengaruh perlakuan durasi fermentasi limbah kantin dengan mikroorganisme EM4 terhadap efektivitas biokonversi limbah kantin menggunakan larva BSF dan juga kandungan kompos yang dihasilkan berdasarkan standar SNI 19-7030-2004. Metode penelitian yang digunakan, yakni berupa kombinasi fermentasi EM4 dengan perbedaan perlakuan durasi (0, 5, dan 10 hari) serta larva BSF sebagai agen pengomposan pada limbah kantin. Analisis data dilakukan terhadap berat biomassa larva BSF dan kompos, substrate consumption, growth rate, bioconversion rate, waste reduction index, serta analisis kandungan unsur hara kompos. Hasil penelitian menunjukkan bahwa efektivitas biokonversi larva BSF tertinggi didapatkan oleh perlakuan durasi fermentasi limbah kantin dengan EM4 selama 10 hari (F10) dengan berat biomassa total larva BSF sebesar 50,09 gram; nilai bioconversion rate sebesar 24,55%; nilai waste reduction index (WRI) sebesar 10,79%; dan berat kompos sebesar 27,38 gram. Sementara itu, sebagian besar kandungan unsur hara kompos pada semua perlakuan sudah sesuai dengan standar SNI 19-7030-2004, kecuali nilai kadar C-organik pada perlakuan F10 serta nilai rasio C/N pada semua perlakuan lainnya (F0, F5, F10, KL dan KF). Pada penelitian ini, didiskusikan juga potensi biokonversi dan peluang bioekonomi pengelolaan limbah kantin FMIPA UI dengan larva BSF.

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Food waste management has become one of the issues at the University of Indonesia due to the lack of an effective and efficient method in managing canteen waste from existing faculties. One common technique in food waste management is composting using larvae of Black Soldier Fly (BSF). The fermentation process and decomposition of complex substances by microorganisms in the organic substrate can optimize the bioconversion efficiency of BSF larvae. However, studies related to the influence of canteen waste fermentation on the effectiveness of bioconversion with BSF larvae have not been discovered to date. Hence, further study regarding this subject is necessary. This study aimed to identify the effects of canteen waste fermentation treatment with EM4 microorganisms on the effectiveness of canteen waste bioconversion using BSF larvae and the content quality of the resulting compost based on the SNI 19-7030-2004 standard. The method used in this study is combining EM4 fermentation with different durations treatment (0, 5, and 10 days) and BSF larvae as composting agent on canteen waste. Data analysis included the weight of BSF larvae biomass and compost, substrate consumption, growth rate, bioconversion rate, waste reduction index, and content quality analysis of the compost. The results showed that the highest effectiveness of bioconversion by BSF larvae was obtained with a 10-day EM4 fermentation treatment (F10). The total biomass of F10 BSF larvae reached 50,09 grams; with bioconversion rate of 24,55%; waste reduction index of 10,79%; and a compost weight of 27,38 grams. Meanwhile, most of the content qualities in the compost for all treatments were following the SNI 19-7030-2004 standard, except for the C-organic content value in the F10 treatment and the C/N ratio values in all other treatments (F0, F5, F10, KL, and KF). In this study, the bioconversion potency and the bioeconomic opportunities of managing the waste from the FMIPA UI canteen using BSF larvae were also discussed."

"Larva lalat tentara hitam (Hermetia illucens L.) atau BSF merupakan agen biologis yang umum digunakan dalam pengelolaan sampah organik. Kemampuan larva lalat tentara hitam dalam mencerna bahan organik didukung oleh berbagai aktivitas bakteri yang hidup di dalam saluran pencernaanya. Pencarian bakteri dengan berbagai aktivitas pendukung pertumbuhan dan proteksi tanaman berpotensi dalam mengurangi dampak akibat penggunaan pupuk dan pestisida sintetis. Penelitian ini dilakukan untuk mengisolasi, menghitung jumlah populasi bakteri, mengetahui potensi aktivitas isolat, serta mengarakterisasi bakteri saluran pencernaan larva BSF. Pengisolasian bakteri dilakukan dengan metode filtrasi membran dengan pengenceran bertingkat dan uji aktivitas dilakukan pada beberapa medium. Berdasarkan morfologi, sebanyak 21 isolat berhasil terisolasi dan hasil perhitungan kepadatan populasi bakteri pada sampel saluran pencernaan BSF, yaitu 1,136 x 1010 CFU/gr. Berdasarkan uji aktivitas isolat, sebanyak dua isolat memiliki aktivitas selulolitik, yaitu isolat G18 dan G21; empat isolat yang dapat mendegradasi kitin, yaitu isolat G9, G16, G18, dan G21; empat isolat dengan kemampuan pelarutan fosfat dan memproduksi IAA, yaitu isolat G7, G10, G13, dan G19. Isolat G18 dan G10 merupakan isolat terunggul karena isolat G18 memiliki keunggulan dalam proteksi tanaman, sedangkan isolat G10 unggul dalam mendukung pertumbuhan tanaman. Isolat G1 hingga G22 memiliki karakteristik yang beraneka ragam. Berdasarkan identifikasi bakteri secara biokimia, isolat G9, G10, G16, dan G18 memiliki karakteristik bakteri dari kelompok Bacillus.

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Black soldier fly (Hermetia illucens L.) larvae are biological agent that commonly used in organic waste management. The ability to digest organic compounds was known to be supported by the diversity of bacterial activities inside their body. The exploration of certain bacterial activities in promoting plant growth and protection may be needed to reduce the impact of synthetic fertilizers and pesticides uses. This study aims to isolate, enumerate the bacterial population from BSF larval gut, screening for potential activities, and characterize isolates. Isolation of bacteria was carried out by membrane filtration method combined with serial dilutions and the bacterial activities carried out on various media. A total of 21 bacteria were isolated from BSF larval gut and the bacterial population density is 1.136 x 1010 CFU/gr. The isolate activity test showed that; two isolates G18 and G21 were positives for cellulolytic activity; four isolates G9, G16, G18, and G21 were able to degrade chitin; four isolates G7, G10, G13, and G19 have the ability to solubilize phosphate and produce IAA. Isolates G18 and G10 were the best isolates because G18 had the advantage in plant protection, while G10 excelled in supporting plant growth. Isolates G1 to G22 had various characteristics. Based on the biochemical identification, isolates G9, G10, G16, and G18 had the characteristics of bacteria from the Bacillus group."

"Pertanian merupakan salah satu sektor utama yang berperan dalam perkembangan ekonomi Indonesia. Produktivitas pertanian dan ketahanan pangan berkaitan erat dengan kondisi kesehatan tanaman. Salah satu patogen penginfeksi tanaman adalah fungi. Pengendali hayati atau biokontrol merupakan teknik pengendalian menggunakan aktivitas metabolisme agen hayati seperti bakteri untuk mengendalikan patogen tanaman. Penelitian terdahulu berhasil mengisolasi bakteri saluran pencernaan black soldier fly larvae (BSFL) dan diketahui mampu mendukung pertumbuhan tanaman. Penelitian ini bertujuan untuk mengetahui kemampuan 10 isolat bakteri saluran pencernaan BSFL terhadap 4 fungi patogen tanaman, yaitu Ganoderma boninense, Fusarium oxysporum, Colletotrichum siamense KA, dan Curvularia lunata BM. Pengujian aktivitas antifungi isolat bakteri dilakukan melalui uji aktivitas antagonistik dan uji aktivitas antibiosis yang dilanjutkan dengan uji aktivitas enzim ekstraseluler isolat potensial. Uji aktivitas antagonistik menggunakan metode dual culture secara kualitatif dan semi-kuantitatif. Uji aktivitas antibiosis menggunakan filtrat medium fermentasi berusia 5 hari yang dicampur dengan bubuk medium potato dextrose agar. Uji aktivitas enzim ekstraseluler isolat potensial meliputi uji enzim protease, lipase, amilase, kitinase, dan selulase menggunakan medium selektif. Hasil uji aktivitas antagonistik menunjukkan 4 dari 10 isolat bakteri saluran pencernaan BSFL, yaitu G7, G17, G20, G21 mampu menghambat pertumbuhan keempat fungi uji. Hasil uji aktivitas antibiosis menunjukkan bahwa efektivitas terbesar diperoleh dari filtrat medium fermentasi isolat G17 dalam menghambat pertumbuhan fungi Ganoderma boninense dengan persentase hambatan sebesar 85,56% – 91,98%. Hasil uji aktivitas enzim menunjukkan isolat G17 memiliki aktivitas enzim ektraseluler protease, lipase, dan kitinase. Isolat G17 teridentifikasi sebagai Stenotrophomonas maltophilia.

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Agriculture is one of the main sectors that play a role in Indonesia's economic development. Agricultural productivity and food security are closely related to plant health conditions. One of the plant infecting pathogens is a fungus. Biological control or biocontrol is a control technique using the metabolic activity of biological agents such as bacteria to control plant pathogens. Previous studies have succeeded in isolating black soldier fly larvae (BSFL) gut bacteria and are known to be able to support plant growth. This study aims to determine the ability of 10 bacterial isolates from BSFL gut against 4 plant pathogenic fungi, namely Ganoderma boninense, Fusarium oxysporum, Colletotrichum siamense KA, and Curvularia lunata BM. Antifungal activity assay of bacterial isolates was carried out through antagonistic activity assay and antibiosis activity assay followed by potential isolates extracellular enzyme activity assay. The antagonistic activity assay used the dual culture method qualitatively and semi-quantitatively. The antibiosis activity assay used 5 days old fermented medium filtrate mixed with potato dextrose agar medium powder. The extracellular enzyme activity assay of potential isolates included protease, lipase, amylase, chitinase, and cellulase enzyme assay using selective media. The results of the antagonistic activity assay showed that 4 out of 10 BSFL digestive tract bacterial isolates namely G7, G17, G20, G21 were able to inhibit the growth of the four tested fungi. The results of the antibiosis activity assay showed that the greatest effectiveness was obtained from the fermented medium filtrate of isolate G17 in inhibiting the growth of the Ganoderma boninense fungus with an inhibition percentage of 85.56% – 91.98%. The results of the enzyme activity assay showed that isolate G17 had extracellular enzyme activity of protease, lipase, and chitinase. Isolate G17 was identified as Stenotrophomonas maltophilia."

"Sebagian besar sampah yang dihasilkan di Indonesia merupakan sampah organik dengan persentase sebesar 58. Kantin Fakultas Teknik Universitas Indonesia FT UI merupakan salah satu penyumbang sampah organik yang cukup tinggi. Pengolahan sampah makanan yang dilakukan masih terbatas pada pengomposan yang membutuhkah waktu 6-7 minggu. Salah satu alternatif pengolahan sampah makanan yang dapat dilakukan yaitu dengan proses biokonversi menggunakan larva Black Soldier Fly Hermetia Illucens. Penelitian ini bertujuan untuk mengetahui timbulan sampah organik Kantin FT UI, menganalaisis durasi optimal biokonversi, serta menganalisis potensi biokonversi dari sampah organik di Kantin FT UI. Penelitian ini dilakukan dengan skala laboratorium yang menggunakan 4 jenis variasi durasi biokonversi yaitu 7 hari, 14 hari, 21 hari, dan 28 hari. Durasi optimal biokonversi yang didapat selanjutnya digunakan untuk mengetahui potensi pengolahan dengan skala eksisting. Dari hasil pengukuran, rata-rata timbulan sampah sebesar 46,27 kg/hari. Selanjutnya dari keempat variasi durasi biokonversi, didapatkan durasi optimal yaitu selama 12 hari. Untuk potensi pengolahan sampah eksisting, proses biokonversi dengan larva Black Soldier Fly Hermetia Illucens dari sampah Kantin FT UI dapat mereduksi sampah sebesar 75 dengan nilai WRI sebesar 6,25 /hari, serta dapat menghasilkan biomassa larva sebanyak 800 gr per 4 kg sampah.

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Most of waste that produced in Indonesia is organic waste with percentage of 58. Canteen of Faculty of Engineering Universitas Indonesia FT UI is one of the high enough contributor of organic waste. Food waste processing is still limited to composting that takes 6 7 weeks. One of the alternative food waste processing that can be applied is bioconversion process using Black Soldier Fly larvae Hermetia Illucens.

This study aims to determine the generation of organic waste in Canteen FT UI, analyze the optimum duration of bioconversion, and analyze the bioconversion potential of organic waste in Canteen FT UI. This study was conducted on a laboratory scale that used 4 variations of bioconversion duration those are 7 days, 14 days, 21 days, and 28 days. The optimum duration of current bioconversion was used to know the potency of processing with the existing scale.

From the measurement result, the average of waste generation is 46,27 kg day. Then from within the variation of bioconversion duration, the optimum duration is 12 days. For the potential of existing waste processing, the bioconversion process with Black Soldier Fly larvae Hermetia Illucens from waste in Canteen of FT UI can reduce organic waste by 75 with WRI value of 6.25 day, and can produce larvae biomass as much as 800 gr per 4 kg organic waste."

"One of the materials with great potency for future nutrition source for animal feed is prepupae of black soldier fly larvae (BSFP) (Hermetia illucens) which is fed on organic wastes. This study was designed to observe the accumulation of specific substance (omega-3) of organic wastes (marine fish offal and tofu dregs) inside harvested biomass of BSFP. A total of 150 gram food consist of marine fish offal and tofu dreg with different proportion (10:90, 25:75, 50:50, and 100% tofu dregs) was fed to black soldier fly larvae (BSFL) for 21 days.At the end of feeding trial, all BSFL were harvested, weighed, and then analyzed for omega-3 fatty acids content. Fatty acid content was analyzed using Gas Cromatography with Flame Ionization Detector (GC-FID). Result of the experiment showed BSFL reared with 25% marine fish offal produced highest biomass (8.1 g / 50 larvae) with least development time (19 days). The total of omega-3 recorded from application of 0%, 10%, 25%, and 50% of marine fish offal was 0.02%, 0.87%, 2.16% and 2.61% in 100 g of dry weight, respectively. This result showed the possibility of transferring specific nutrient from organic wastes biomass of BSFP which provides base knowledgement for further application in design of specific animal feed from BSFP."

"Pertanian merupakan salah satu sektor yang mendukung dalam pemenuhan kebutuhanpangan di Indonesia. Peningkatan jumlah penduduk yang ada di Indonesia setiap tahuntidak sebanding dengan hasil panen pertanian yang semakin menurun. Pemilihan pupukkimia untuk meningkatkan hasil pertanian dinilai tidak efektif karena dinilai tidak ramahlingkungan. Salah satu cara alternatif untuk meningkatkan hasil panen adalah denganmemanfaatkan bakteri sebagai pendukung pertumbuhan tanaman atau Plant GrowthPromoting Bacteria (PGPB). Penelitian ini bertujuan mendapatkan bakteri yangberpotensi sebagai pendukung pertumbuhan tanaman meliputi kemampuan dalamproduksi Indole Acetic Acid (IAA), pelarutan fosfat, kitinase, dan selulase. Larva lalattentara hitam dinilai dapat menjadi sumber bakteri karena kemampuan konversi biowasteyang relatif cepat, sehingga diduga berkorelasi dengan bakteri yang terdapat di larva.Isolasi bakteri dari larva lalat tentara hitam dilakukan pada fase larva instar I dan Vdengan 4 variasi medium isolasi (Casein Starch Agar, Carboxymethyl Cellulose agar,Yeast Extract-suplemented Minimal agar, dan Skim Milk Agar). Setiap isolat yang telahdidapat telah berhasil dilakukan penapisan produksi IAA, aktivitas pelarutan fosfat,aktivitas enzim selulolitik, dan aktivitas enzim kitinolitik. Selain itu, setiap isolat telahdilakukan karakterisasi secara morfologi dan sifat biokimia. Sebanyak 22 isolat telahdidapatkan dengan kode B1-B22, 5 (B1, B11, B15, B16, dan B21) dari seluruh isolattersebut memiliki hasil positif pada dua penapisan uji aktivitas dan 9 isolat memiliki hasilpositif pada satu penapisan uji aktivitas. Isolat B21 merupakan isolat terbaik karenamemiliki hasil positif pada dua uji (uji aktivitas enzim selulolitik dan kitinolitik) danmemiliki nilai tertinggi pada kedua uji tersebut. Isolat B21 diduga berasal dari dari genusGardnerella atau Haemophilus berdasarkan tabel identifikasi.

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Agriculture is one of the sectors that support the fulfillment of food demand in Indonesia.

Increasing in the number of people in Indonesia every year is not proportional to the

decreasing agricultural yields. The use of chemical compounds to increase agricultural

yields is considered ineffective because it can damage the surrounding environment. One

alternative way to increase crop yields is to use bacteria as Plant Growth Promoting

Bacteria (PGPB). This study aims to obtain bacteria that have the potential to support

plant growth including the ability to produce Indole Acetic Acid (IAA), phosphate

solubilization, chitinase, and cellulase. Black soldier fly (BSF) larvae are considered a

source of bacteria because their role in bioconversion of organic waste are thought to be

correlated with bacteria in BSF larvae. Isolation of bacteria from BSF larvae was carried

out using larval stages instars I and V with 4 variations of isolation medium (Casein

Starch Agar, Carboxymethyl Cellulose agar, Yeast Extract-supplemented Minimal agar,

and Skim Milk Agar). Isolates have been successfully screened for IAA production,

phosphate solubilization activity, cellulolytic enzyme activity, and chitinolytic enzyme

activity. In addition, each isolate has been characterized by morphological

characterization and biochemical tests. A total of 22 isolates were obtained with codes

B1-B22, five isolates (B1, B11, B15, B16, and B21) of these isolates had positive results

in two activity tests and nine isolates had positive results in one activity test. Isolate B21

was the best isolate because the isolate showed positive results in two tests (cellulolytic

and chitinolytic enzyme activity tests) and had the highest score in both tests. The isolate

B21 is believed to be a member of the genus Gardnerella or Haemophilus based on the

identification table."