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"Tujuan : Penelitian ini bertujuan untuk mengamati ketahanan korosi dari TAD berbahan SS setelah pemaparan pada tiga jenis larutan kumur yang berbeda yang ditinjau dari permukaan topografi dan komposisi atomik. Metode : 28 unit TAD berbahan Stainless Steel dibagi secara merata ke dalam 4 kelompok larutan kumur (sodium fluoride 0,2%, povidone iodine 1%, kitosan 1,5%, dan air destilasi) yang masing-masing terdiri dari 7 unit TAD. Setelah 3 bulan perendaman dilakukan evaluasi ketahanan korosi material TAD SS menggunakan scanning electrone microscope (SEM) untuk melihat topografi permukaan dan energy-dispersive x-ray spectroscopy (EDS) untuk melihat komposisi atomik pada permukaan logam TAD SS. Hasil : Uji SEM menunjukkan tidak terdapat perbedaan bermakna antara permukaan TAD SS setelah pemaparan dalam larutan sodium fluoride, povidone iodine, dan larutan kontrol (air destilasi) yaitu permukaan menjadi kasar dan terbentuk korosi lubang/intergranular. Namun pada TAD SS yang direndam dalam larutan kitosan hanya mengalami perubahan permukaan menjadi kasar tanpa disertai korosi lubang/intergranular. Sementara uji EDS menunjukkan tidak terdapat perbedaan antara komposisi atomik TAD berbahan logam stainless steel setelah dipaparkan dalam larutan Fluoride, povidone iodine, kitosan, dan air destilasi (kontrol). Kesimpulan : Perendaman TAD SS 316L pada ketiga larutan kumur memicu proses korosi yang terlihat dari kekasaran permukaan logam paska perendaman, dengan larutan sodium fluoride dan povidone iodine bersifat lebih korosif, sementara larutan kitosan yang paling tidak korosif. TAD SS 316L memiliki biokompatibilitas yang baik terlihat dari pelepasan ion nikel dan kromium yang minimal pada seluruh sampel TAD SS paska perendaman.

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Objectives : This study aims to evaluate the corrosion resistance of stainless steel TAD after immersion in three mouthwash solutions marked by topography surface and atomic composition. Methods : 28 unit stainless steel TADs were divided into 4 group of mouthwashes (0,2% sodium fluoride, 1% povidone iodine, 1,5% chitosan, and distilled water as control group) each consisting of 7 TADs. After 3 months of immersion, the corrosion resistance of SS TAD will be evaluated using scanning electron microscope (SEM) to analyze the surface topography and energy-dispersive x-ray spectroscopy (EDS) to analyze the atomic composition. Results: SEM images showed no significant difference between the surface topography of SS TAD after immersion in sodium fluoride, povidone iodine, and distilled water as they exhibit surface roughness and the presence of pitting/intergranular corrosion. However, SS TAD immersed in chitosan solution only displayed surface roughness without any sign of pitting/intergranular corrosion. EDS examination showed no significant difference between the atomic composition of SS TAD immersed in all mouthwash solutions. Conclusions : Immersion of SS TAD 316L in three different mouthwashes induced corrosion process which is shown by the surface roughness after 3 months of immersion. Sodium fluoride and povidone iodine mouthwash have shown to be more corrosive, while chitosan mouthwash was the least corrosive. SS TAD 316L displayed good biocompatibility which is shown by minimal release of nickel and chromium ions on all TAD samples after immersion"

"Kulit merupakan sistem pertahanan eksternal, langsung menjalani proses penyembuhan luka ketika terjadi luka dan banyak orang cenderung memberi proses penyembuhan luka dengan agen antiseptik, povidone iodine 10 Betadine . Namun, terdapat ide baru tentang penggunaan povidone iodine 5 pada penyembuhan luka kulit yang dapat memberikan efek yang berbeda. Tujuan dari penelitian ini adalah untuk membandingkan efek berbeda dari penggunaan konsentrasi yang berbeda dari povidone iodine pada jumlah PMN, fibroblast, dan serat kolagen dan untuk menentukan kadar 5 atau 10 yang lebih cocok untuk digunakan. Penelitian ini menggunakan tikus sebagai sampel, masing-masing tikus diberikan 3 luka dengan 3 perlakuan berbeda terdiri dari kontrol, povidone iodine 10 , dan povidone iodine 5 . Pada hari ke-3, tiga tikus pertama dikorbankan dan pada hari ke-7 3 tikus berikut dikorbankan, lalu dibuat spesimen histologi dengan mengambil area luka dan diwarnai dengan Hematoksilin-Eosin untuk menganalisis jumlah PMN dan fibroblast, serta Van Gieson menganalisis serat kolagen. Hasil dari penelitian ini memperlihatkan tidak ada perbedaan yang signifikan antara povidone iodine 5 dan 10 dalam proses keseluruhan penyembuhan luka yang dilihat dari jumlah PMN, fibroblast, dan serat kolagen.

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Skin is an external defense system, directly undergo wound healing process when scars occur and people tend to interfere the wound healing process with antiseptic agents, in this case is the use of povidone iodine 10 Betadine . However, there is new idea about the appliance of povidone iodine 5 on cutaneous wound healing may give different effect. This research aims to compare the different effect of using different concentration of povidone iodine on number of PMN, fibroblast, and collagen fibers during wound healing process and to determine which one is more suitable to use.

This experiment using rats as samples, each rat is given 3 wounds with 3 different treatments consisted of control, povidone iodine 10, and povidone iodine 5. On the 3rd day, the first three rats were sacrificed and on the 7th day the following 3 rats were sacrificed, then made histological specimens by taking the wound area and stained it using Hematoxylin eosin to analyze number of PMN and fibroblast, also Van Gieson to analyze collagen fibers. The result of this experiment is that there is no significant difference among povidone iodine 5 and 10 in overall process or phases of wound healing, as seen from number of PMN, fibroblast, as well as collagen fibers. "

"In deep periodontal lesions, scaling and root planning (SRP) failed to complete elimination of periodontal bacteria, so chemical antimicrobial agents are used topically to destroy microorganism. Povidon-iodin 10% is one of antimicrobial agents that can be applied topically anddirectly in the pocket. The aim of the research were evaluated the efficacy of povidon-iodin l0% as chemical antimicrobial agents locally applied into periodontal pocket. The data are obtained from patients with chronic adult periodontitis baseline periodontal pocket depth (PPD) are 5-7 mm. The teeth are scaled and root planed after clinical examinations ( plaque index, papilla bleeding index and periodontal pocket depth ) and test sites or control sites are assisned randomly. Topically application of povidon-iodin 10% at test sites and aquabides at control sites is applied at day 1st and day 7th. The clinical parameters are assessed at day 14th. The results of the research showed that application of povidon-iodin l0% afer SRP provide statistically significant more favorable papilla bleeding index reduction than SRP + aquabides after 14 days. The pocket depth reduction at testsites are greater than control sites ( baseline PPD=6 and 7 mm). The conclusions of the research showed that application subgingival povidon-iodin 10% as adjunctive to SRP significantly reducePBI and PPD ( 6 & 7 mm ) than without application povidon-iodin 10%."

"Latar Belakang: Pasien perawatan ortodonti pada umumnya dianjurkan menggunakan obat kumur berfluoride untuk menjaga kebersihan rongga mulut dan mencegah terjadinya karies. Namun, Fluoride dapat mempengaruhi karakteristik kawat ortodonti Stainless Steel yang digunakan selama perawatan. Belum diketahui efek pemakaian obat kumur berfluoride terhadap kekuatan tarik kawat ortodonti Stainless Steel. Tujuan: Mengetahui efek pemakaian obat kumur berfluoride terhadap kekuatan tarik kawat ortodonti Stainless Steel. Metode: Menguji kekuatan tarik kawat ortodonti Stainless Steel 0,016 inci setelah dilakukan perendaman pada 100 ml obat kumur berfluoride 0,05 selama 30, 60, dan 90 menit. Hasil: Tidak terdapat perbedaan yang bermakna secara statistik dari kekuatan tarik kawat ortodonti Stainless Steel setelah direndam obat kumur berfluoride. Nilai p pada perendaman obat kumur berfluoride selama 30, 60, dan 90 menit masing-masing adalah 0,790; 0,742; dan 0,085 nilai p > 0,05. Kesimpulan: Pemakaian obat kumur berfluoride tidak mempengaruhi kekuatan tarik kawat ortodonti Stainless Steel.

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Background: Patients with orthodontic treatment are commonly recommended to use Fluoride mouthwash for maintaining their oral hygiene and preventing dental caries. However, Fluoride may affect the characteristics of Stainless Steel orthodontic archwires that used during the treatment. The effect of Fluoride mouthwash on tensile strength of Stainless Steel orthodontic archwires is still unknown.

Purpose: To know the effect of Fluoride mouthwash on tensile strength of Stainless Steel orthodontic archwires.

Method: Examine the tensile strength of Stainless Steel orthodontic archwires 0,016 inch after immersed in 100 ml Fluoride mouthwash 0,05 for 30, 60, and 90 minutes.

Result: There is no statistically significant difference on tensile strength of Stainless Steel orthodontic archwires after immersed in Fluoride mouthwash. The p values on immersion Fluoride mouthwash for 30, 60, and 90 minutes consecutively are 0,790 0,742 and 0,085 p value 0,05.

Conclusion: The using of Fluoride mouthwash didn rsquo t have an effect on tensile strength of Stainless Steel orthodontic archwires."

"Latar belakang: Penelitian sebelumnya banyak melihat pengaruh paparan obat kumur dan aplikasi bleaching terhadap kekasaran permukaan nanohibrida dan mikrohibrida, sedangkan nanofil dan bulkfil merupakan restorasi estetika. Oleh karena itu, dilakukan pengaruh paparan obat kumur klorheksidin 0,2% dan povidone iodine 1% selama penggunaan 14 hari sesuai aturan pakai lalu dilakukan aplikasi bleaching karbamid peroksida 10% selama 4jam/hari dalam 14 hari. Tujuan: Menganalisa pengaruh aplikasi bleaching karbamid peroksida 10% terhadap kekasaran permukaan resin komposit nanofil dan bulkfil setelah paparan obat kumur klorheksidin 0,2% dan povidone iodine 1%. Metode: Spesimen resin komposit nanofil dan bulkfil (n=24) dibagi ke dalam 4 kelompok, lalu masing-masing resin komposit direndam obat kumur klorheksidin 0,2% (n=6) dan povidone iodine 1% (n=6) selama 14 hari pemakaian dan diaplikasikan bleachingkarbamid peroksida 10% selama 4 jam/hari dalam waktu 14 hari. Pengukuran kekasaran permukaan dilakukan sebelum dan setelah perendaman obat kumur, serta setelah bleaching menggunakan surface roughness tester (Surtronic® S-128).Hasil: Kekasaran permukaan resin komposit nanofil dan bulkfil setelah aplikasi bleaching berbeda bermakna (p<0,05), dalam hal ini antara sebelum perendaman dan setelah perendaman obat kumur berbeda bermakna (p<0,05), tetapi antara setelah perendaman obat kumur dan setalah aplikasi bleaching tidak berbeda bermakna (p>0,05). Kesimpulan: Resin komposit nanofil dan bulkfil mengalami peningkatan kekasaran permukaan setelah terpapar obat kumur klorheksidin 0,2% dan povidone iodine 1% serta setelah aplikasi bleaching karbamid peroksida 10%. Peningkatan kekasaran permukaan nanofil lebih tinggi dari peningkatan kekasaran bulkfil.

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Background: Previous studies have researched the effect of mouthwash exposure and bleaching application on the surface roughness of nanohybrid and microhybrid, while nanofil and bulkfil are aesthetic restorations. Therefore, the effect of exposure to 0.2% chlorhexidine and 1% povidone iodine mouthwash was carried out for 14 days according to the instructions of use, then 10% carbamide peroxide bleaching was applied for 4 hours/day for 14 days. Objective: To analyze the effect of 10% carbamide peroxide bleaching application on the surface roughness of nanofil and bulkfil composite resins after exposure to 0.2% chlorhexidine and 1% povidone iodine mouthwash. Methods: Nanofill and bulkfill composite resin specimens (n=24) were divided into 4 groups, then each composite resin was immersed in 0.2% chlorhexidine (n=6) and 1% povidone iodine (n=6) mouthwash for 14 days of use and 10% carbamide peroxide bleaching was applied for 4 hours/day for 14 days. Surface roughness measurements were carried out before and after immersion in mouthwash, and after bleaching using a surface roughness tester (Surtronic® S-128) Results: The surface roughness of nanofill and bulkfill composite resins after bleaching application was significantly different (p<0.05), in this case between before and after immersion in mouthwash was significantly different (p<0.05), but between after immersion in mouthwash and after bleaching application there was no significant difference (p>0.05). Conclusion: Nanofil and bulkfil composite resins have increased surface roughness after exposure to 0.2% chlorhexidine and 1% povidone iodine mouthwash and after application of 10% carbamide peroxide bleaching. The increase in nanofil surface roughness was higher than the increase in bulkfil surface roughness."

"Bau mulut adalah masalah yang dapat membuat orang tidak nyaman. Stelechocarpus burahol (kepel) terbukti secara empiris dapat menghilangkan bau mulut, karena mengandung flavonoid yang berfungsi sebagai agen pengadsorbsi. Untuk meningkatkan potensinya dilakukan ekstraksi dari buah kepel. Untuk mendapatkan hasil yang baik, ekstrak distandardisasi. Standardiasi yang dilakukan antara lain parameter spesifik kadar total flavonoid adalah 0,30 %, kadar total fenol adalah 7,85 g GAE/100 g, kadar logam berat adalah 0 ppm, dan kadar sisa pelarut adalah 0 ppm, sedangkan pada parameter non spesifik berupa kadar abu adalah 94,95 %, kadar abu tidak larut asam adalah 0,97 %, kadar air adalah 28,36 %, uji kelarutan ekstrak 1:10. Ekstrak dibuat menjadi sediaan obat kumur agar mudah digunakan sehari-hari. Sediaan obat kumur ini mengandung ekstrak buah kepel, isomaltulosa, mentol, asam malat, natrium benzoat, sorbitol, dan etanol. Pada formulasi I, II, dan III secara berturut-turut memiliki pemanis isomaltulosa 4 %, 8 %, dan 12 %, dari ketiga formulasi tersebut diuji untuk menentukan formulasi yang paling disukai dan stabil. Formula II mendapatkan nilai rata-rata tertinggi pada parameter warna dan aroma, serta mendapat peringkat kedua pada parameter rasa, sedangkan pada pengujian fisik obat kumur, pada formulasi I memiliki bau mentol; jernih; warna incosete 0605 orange; pH 5,35; dan bobot jenis 1,0349; formula II memiliki bau mentol; jernih; warna incosete 0605 orange; pH 5,36; dan bobot jenis 1,1234; formula III memiliki bau mentol; jernih; warna incosete 0605 orange; pH 5,37; dan bobot jenis 0,9953.

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Halitosis is a problem that can make people uncomfortable. Stelechocarpus burahol (Kepel) empirically proven to eliminate bad breath, because it contains flavonoid which serves as an adsorbent agent. To improve the potency, kepel fruit is extracted. To get a good result, standardized extract. Standardization is carried out include specific parameters such as levels of total flavonoids was 0.30%, the total phenol content was 7.85 g GAE/100 g, heavy metal content is 0 ppm, and levels of residual solvent was 0 ppm, whereas the non-specific parameters form of ash content is 94.95%, acid insoluble ash content was 0.97%, the water content was 28.36%, solubility test extract 1:10. Extracts made ​​into a mouthwash preparations for daily use. This mouthwash contains fruit extracts Kepel, isomalt, menthol, malic acid, sodium benzoate, sorbitol and ethanol. In the formulations I, II, and III consecutive had a sweetener isomalt 4%, 8%, and 12%, all three formulations were tested to determine the most preferred formulation and stable. Formulation II get the highest average score on the parameters of color and aroma, and was ranked second in the taste parameters, whereas the physical testing of mouthwash, the formulation I have a menthol smell, transparant, 0605 incosete orange color, pH 5.35, and a specific gravity of 1.0349, formulation II has the smell of menthol, transparant, 0605 incosete orange color, pH 5.36, and a specific gravity of 1.1234, formulation III has a menthol odor, transparent, 0605 incosete orange color, pH 5.37, and a specific gravity of 0.9953."

"Penelitian ini membuktikan efektifitas teri jengki (Stolephorus insularis) sebagai fluoridasi gigi dengan acuan kedalaman intrusi fluor. Digunakan metode eksperimental laboratorik in vivo. Subjek 14 ekor tikus Sprague dawley dibagi menjadi kelompok baseline, kontrol negatif pakan, kontrol negatif oles, metode pakan teri, dan metode oles larutan teri. Setelah perlakuan 15 hari, gigi dipotong transversal, diproses untuk uji intrusi fluor menggunakan mikroskop fluoresensi. Didapatkan hasil peningkatan intrusi fluor pada kelompok eksperimental dibandingkan kontrol negatif (p<0,05). Intrusi fluor metode oles lebih tinggi dibandingkan metode pakan (p <0,05). Jadi, aplikasi teri jengki, baik lewat pengunyahan maupun pengolesan, meningkatkan intrusi fluor pada email.

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The effectiveness of anchovy (Stolephorus insularis) as a fluoridative agent is measured by depth of fluoride intrusion. This study used experimental laboratory method. 14 Sprague Dawley rats were divided into groups of baseline, experimental (feeding and smearing), and their negative controls. After 15 days, teeth were cut transversely and fluoride intrusions were observed using fluorescence microscope. There were increased fluoride intrusion in enamel of experimental groups compared to their negative controls (p<0.05). Fluoride intrusion of smearing group is higher than feeding group (p <0.05). Thus, application of anchovy substrate, either by chewing or smearing, increases fluoride intrusion in tooth enamel."

"Menganalisis sitotoksisitas TAD SS setelah paparan tiga jenis larutan kumur yang dilihat melalui parameter reaktivitas dan viabilitas sel fibroblast BHK-21. Metode: 28 unit TAD SS dibagi menjadi masing-masing 7 unit TAD dalam 4 kelompok larutan kumur (sodium fluoride 0.2%, povidone iodine 1%, kitosan 1.5%, akuades) dan direndam selama 90 hari. Elusi TAD SS dalam larutan kumur kemudian dimasukkan ke dalam kultur sel fibroblast BHK-21 dan diinkubasi selama 24 jam. Reaktivitas dianalisis dengan mikroskop inverted-light dan reliabilitasnya diuji dengan ICC serta diuji secara statistik dengan independent T-test. Viabilitas dilakukan dengan metode MTT Assay dan dianalisis secara statistik dengan independent T-test. Hasil: Sel fibroblast BHK-21 menunjukkan perbedaan reaktivitas yang bermakna (p<0.05) setelah paparan elusi TAD SS dalam larutan kumur povidone iodine dan kitosan dibanding larutan povidone iodine dan kitosan tanpa TAD SS. Uji viabilitas menunjukkan perbedaan bermakna (p<0.05) setelah paparan elusi TAD SS dalam larutan kumur povidone iodine dibanding larutan povidone iodine saja tanpa TAD SS. Reaktivitas sel fibroblast BHK-21 pada kelompok yang terpapar larutan fluoride dan akuades tidak berbeda bermakna (p>0.05) dibanding larutan fluoride dan akuades tanpa TAD SS. Viabilitas sel fibroblast BHK-21 pada kelompok yang terpapar larutan fluoride dan akuades tidak berbeda bermakna (p>0.05) dibanding kelompok larutan fluoride dan akuades tanpa TAD SS. Kesimpulan: Pasien ortodonti dengan TAD SS dapat dianjurkan menggunakan larutan kumur kitosan dengan kadar lebih rendah atau akuades. Larutan kumur fluoride tidak dianjurkan untuk diberikan pada pasien ortodonti dengan TAD SS. Larutan povidone iodine sebaiknya tidak digunakan secara terus menerus untuk memberi waktu bagi revitalisasi sel.

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Analyzing the cytotoxicity of SS TAD after exposure to three types of mouthwash seen through the parameters of reactivity and viability of BHK-21 fibroblast cells. Methods: 28 units of SS TAD were divided into 7 units each of TAD in 4 groups of mouthwash (sodium fluoride 0.2%, povidone iodine 1%, chitosan 1.5%, aquadest) and immersed for 90 days. The elution of SS TAD in mouthwash was then fed into BHK-21 fibroblast cell culture and incubated for 24 hours. Reactivity was analyzed with an inverted-light microscope and reliability was tested with ICC also statistically tested with an independent T-test. Viability was performed using MTT Assay and statistically analyzed with an independent T-test. Results: BHK-21 fibroblast cells showed significant differences in reactivity (p<0.05) after exposure to TAD SS elution in povidone iodine and chitosan mouthwash compared to its solution groups without TAD SS. Viability test showed significant differences (p<0.05) after exposure to TAD SS elution in povidone iodine mouthwash compared to povidone iodine without TAD SS. The reactivity of BHK-21 fibroblast cells exposed to fluoride and aquadest was not significantly different (p>0.05) compared to its solution groups without TAD SS. The viability of BHK-21 fibroblast cells exposed to fluoride and aquadest did not significantly different compared to its solution groups without TAD SS. Conclusions : Orthodontic patients with TAD SS may be recommended to use lower levels of chitosan mouthwash or aquadest. Fluoride mouthwash is not recommended to be given to orthodontic patients with TAD SS. Povidone iodine solution should not be used continuosly to give time for cell revitalization"

"Tujuan: Mini implan ortodontik berbahan titanium alloy sebagai penjangkaran skeletal diketahui memiliki ketahanan korosi yang tinggi, namun beberapa penelitian menemukan adanya perubahan ketahanan korosi setelah berkontak dengan larutan kumur. Penelitian ini bertujuan untuk menganalisis perubahan topografi permukaan dan perubahan komposisi elemental mini implan ortodontik titanium alloy setelah pemaparan dengan tiga jenis larutan kumur. Metode: Sebanyak 28 mini implan ortodontik dibagi menjadi empat kelompok secara merata dan direndam selama 28 hari dalam larutan klorheksidin glukonat 0.2%, sodium fluoride 0.2%, dan kitosan 1.5%, dan air destilasi. Topografi permukaan bagian kepala dan leher mini implan ortodontik diperiksa menggunakan scanning electron microscopy (SEM) dan komposisi elemental dinilai menggunakan energy-dispersive x-ray spectroscopy energi (EDS). Hasil: Topografi permukaan mini implan ortodontik pada semua kelompok menunjukkan beberapa iregularitas permukaan karena cacat manufaktur, tetapi tidak ditemukan korosi celah maupun korosi lubang. Mini implan ortodontik yang direndam dalam kitosan menunjukkan permukaan yang lebih halus. Komposisi elemental hanya menunjukkan perbedaan bermakna pada elemen titanium dan aluminium antara kelompok sodium fluorida dan kitosan. Kesimpulan: Mini implan ortodontik titanium alloy menunjukkan ketahanan korosi yang baik setelah pemaparan dalam larutan klorheksidin glukonat, sodium fluoride, dan kitosan selama 28 hari. Mini-implan ortodontik yang direndam dalam kitosan menunjukkan permukaan yang lebih halus dan komposisi elemen titanium dan aluminium yang lebih tinggi dibandingkan kelompok lain.

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Objectives: Orthodontic mini-implants are widely used as an intraoral skeletal anchorages. Titanium alloy orthodontic mini-implants are known to have high corrosion resistance, but studies have found some corrosion behavior after contact with mouthwashes. The current in- vitro study aimed to examine surface topography and elemental composition as parameters of corrosion resistance for titanium alloy orthodontic mini-implants after being immersed in three different types of mouthwashes. Methods: A total of 28 titanium alloy orthodontic mini- implants were divided equally into four groups and immersed for 28 days in chlorhexidine gluconate 0.2% mouthwash, sodium fluoride 0.2% mouthwash, chitosan 1.5% mouthwash, and distilled water. All the orthodontic mini-implants’ heads and necks were then examined for surface topography using a scanning electron microscopy (SEM) and the elemental composition was assessed using energy-dispersive x-ray spectroscopy (EDS). Results: Surface topography of the orthodontic mini-implants immersed in chlorhexidine gluconate, sodium fluoride, chitosan, and distilled water exhibited some manufacturing defects and rough surfaces, but no signs of crevices or pitting corrosion on the heads and necks. The elemental composition of all groups was comparable, but there was a statistically significant difference between titanium and aluminum (at%) between the sodium fluoride group and the chitosan group. Conclusion: Titanium alloy orthodontic mini-implants exhibited good corrosion resistance after immersion for 28 days in chlorhexidine gluconate 0.2%, sodium fluoride 0.2%, and chitosan 1.5%. Orthodontic mini-implants immersed in chitosan showed a smoother surface and higher titanium and aluminum (at%) than other groups."

"Temporary anchorage device TAD yang akan digunakan kembali akibat kegagalan pemasangan reinsertion atau perubahan lokasi pemasangan relocation harus melalui proses sterilisasi ulang. Bakteri Porphyromonas gingivalis PG adalah salah satu bakteri yang ditemukan pada daerah peri ndash; implantitis. Penelitian ini bertujuan untuk mengetahui efek antibakteri nanopartikel perak terhadap PG pada TAD yang disterilisasi ulang menggunakan larutan nanopartikel perak P1 dibandingkan teknik autoclave P2 . Sebanyak 10 buah sampel pada masing ndash; masing kelompok direndam dalam larutan plak buatan dengan dominasi koloni PG ATCC 33277 selama 24 jam dalam suasana anaerob. Sampel kemudian diusap dan dibiakkan pada brusella agar darah selama 24 jam dalam suasana anaerob. P1 disterilisasi ulang dengan direndam dalam larutan nanopartikel perak selama 180 menit, P2 disterilisasi ulang dengan autoclave selama 40 menit pada suhu 1210C 2500F . Setelah sterilisasi ulang, sampel diusap dan dibiakkan kembali dengan teknik yang sama. Koloni PG sebelum dan setelah perlakuan 103 CFU / mL dihitung menggunakan Electronic Colony Counter ECC . Hasil penelitian ini menunjukkan bahwa larutan nanopartikel perak memiliki efek yang sama baiknya dengan autoclave terhadap PG.

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Objective The aim of this study was to evaluate the number of Porphyromonas gingivalis PG colonies in used Temporary Anchorage Device TAD ndash for relocating or reinserting as the antimicrobial effect of silver nanoparticles solution compared with autoclave re sterilization technique. Materials and Methods Samples were 20 new TADs which separated into 2 groups, P1 and P2. Before re ndash sterilized, samples were immersed in a plaque forming solution dominated with PG ATCC 33277 and cultured under anaerobic condition for 24 hours. The material was obtained from samples using sterile cotton pellet and cultured on Brusella agar plate for 24 hours under anaerobic condition. P1 was re sterilized by silver nanoparticle solution for 180 minutes and P2 was re sterilized using autoclave for 40 minutes in 1210C 2500F . The cultured steps above were repeated to get the number of surviving PG colonies after re sterilization. The number of PG colonies were counted using Electronic Colony Counter ECC . Their antimicrobial activity was evaluated by comparing the number of PG colonies 103 CFU mL before and after re sterilization. Results No surviving PG colony existed of Brusella agar plate on both group after re ndash sterilized. Conclusions Silver nanoparticle solution is as effective as autoclave to againts PG."