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Dupont, Anton
"The statement contained in the motto cited above, made as early as the year 1906, still has lost nothing of its value for the present time. Although innumerable investigations have been carried out on proteins, and much insight was gained from this, a lot of problems connected with the proteins, remain to be solved. As is well known, proteins are found in nature in the animal and vegetable kingdoms. The differences between these two groups of proteins are situated in the number and the amount of the various acids present in these proteins.
Up to comparatively recent years, however, the knowledge of the requirement for the amino acids in the diet was limited to in-formation obtained with the young rat. This animal was able to grow when receiving only nine amino acids: namely histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine; and grew significantly better when arginine was also provided. These ten amino acids were called "essential" for the growing rat, the remaining amino acids were called "non essential". However, there was no implication that the dietetically non-essential amino acids are of little or no value. In spite of the cautions issued, there has been a general tendency to assume, that the above classification has a very broad application. The investigations made by Rose et all. (1,2,3,4,5), have revealed that for adult man only eight amino acids are essential, namely, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine. Moreover in these investigations, it was demonstrated, that only foods with sufficient caloric and nitrogen supply and adequate amounts of these eight amino acids simultaneously are able to main﷓ thin a nitrogen balance. This fact is of far reaching importance, because, from this it follows, that the nutritive value of a meal, with respect to the proteins, is determined by the total amount of essential amino acids - in the proper proportions - present in the constituents of the meal. In Indonesia, rice is the most important constituent of a meal. However rice contains such a small amount of some essential amino acids, that even larger use is not sufficient to meet the requirements of man. Therefore, other foods, which complete this shortage, must be used in combination with rice.
In general, in Indonesia, foods containing animal protein are relatively scarce; therefore, it is necessary to replace these with foods containing vegetable protein. An important source of plant protein is found in the soybean. As a general rule, the soybean yields fair crops, without asking too much care. Botanically, the soybean has been referred to as Glycine hispida (Moench) Max. Recent studies indicate that the correct botanical name should be Glycine max (L.) Merril according to international botanical rules (6).
The early history of the soybean, like that of most important food crops, is lost in obscurity. In the Far East story tellers for centuries have related, tales of the remarkable history of the soybean. Ancient Chinese literature reveals that the soybean was extensively cultivated and highly valued as a food. There it is said to be one of the grains planted by one of the gods of agriculture, named Hou Tsi. The first written record of the plant is contained in the books Pen Tsao Kong Mu, a materia medica written by Emperor Sheng Nung in 2838. B.C.
In many of the early writings advice of agricultural experts is given on soil preferences, proper time of planting, methods and rates of planting, the best varieties to plant under different conditions and for different uses, time to harvest, methods of storage, and utilization of the many varieties for different purposes. Some of this expert advice goes as far back as 2207 B.C. The soybean was included in the second class of drugs, and was regarded as having many medicinal virtues; it was regarded as a specific remedy for the propel functioning of the heart, and other organs."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 1954
D114
UI - Disertasi Membership  Universitas Indonesia Library
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London: Academic Press, 1991
581.19 MET
Buku Teks  Universitas Indonesia Library
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London: Academic Press, 1991
581.19 MET
Buku Teks  Universitas Indonesia Library
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Ria Andreinie
"Latar belakang: Indonesia dihadapkan pada masalah stunting yang cukup tinggi. Anak yang menderita stunting memiliki konsentrasi asam amino esensial yang lebih rendah. Mechanistic Target of Rapamycin Complex 1 (mTORC1) sebagai jalur utama regulasi pertumbuhan yang sensitif terhadap asam amino, mempromosikan sintesis protein melalui S6K1 dan 4EBP1. Penelitian ini bertujuan menganalisis keterlibatan asam amino esensial pada kadar mTORC1, S6K1, dan 4EBP1 dan korelasinya dengan kejadian stunting. Metode: Desain penelitian adalah kasus-kontrol. Anak berusia 6-24 bulan di Provinsi Sumatera Selatan diambil secara cluster random sampling berjumlah 137 orang. Pengumpulan data menggunakan kuesioner dan pengukuran antropometri. Pemeriksaan feses secara makroskopis dan mikroskopis, pemeriksaan darah dengan teknik LC-MS dan ELISA. Hasil: Terdapat perbedaan bermakna dalam hal usia anak, panjang badan lahir, BB/U, dan LK/U. Tidak ditemukan perbedaan makroskopis dan mikroskopis feses di antara kedua kelompok. Asam amino triptofan berbeda bermakna (p=0,004) dan berhubungan dengan kejadian stunting (p=0,045). Kadar mTORC1 dengan S6K1 dan 4EBP1 tidak berbeda pada kedua kelompok. Kadar mTORC1 tidak berhubungan dengan kejadian stunting. Kadar asam amino lisin berhubungan bermakna dengan kadar mTORC1 (p=0,003). Kadar mTORC1 berhubungan bermakna dengan kadar S6K1 dan 4EBP1 (masing-masing p<0,001). Simpulan: Asam amino esensial berimplikasi pada kejadian stunting dan kadar mTORC1, mTORC1 memengaruhi kadar S6K1 dan 4EBP1 anak.

Background: Indonesia is faced with a fairly high stunting problem. Children who suffer from stunting have lower concentrations of essential amino acids. The Mechanistic Target of Rapamycin Complex 1 (mTORC1) as the main pathway of growth regulation that is sensitive to amino acids, promotes protein synthesis through S6K1 and 4EBP1. This study aims to analyze the involvement of essential amino acids in mTORC1, S6K1, and 4EBP1 levels and their correlation with stunting. Methods: The study design was a case-control study. Children aged 6-24 months in South Sumatra Province were taken using cluster random sampling totaling 137 people. Data collection used questionnaires and anthropometric measurements. Macroscopic and microscopic examination of feces, and blood examination using LC-MS and ELISA techniques. Results: Significant differences were in child age, birth length, BB/A, and LK/A. There were no macroscopic and microscopic differences in feces between the two groups. Tryptophan amino acids were significantly different (p=0.004) and associated with stunting (p=0.045). mTORC1 levels with S6K1 and 4EBP1 were not different in both groups. mTORC1 levels were not associated with stunting. Lysine amino acid levels were significantly associated with mTORC1 levels (p=0.003). mTORC1 levels were significantly associated with S6K1 and 4EBP1 levels (each p<0.001). Conclusion: Essential amino acids are implicated in stunting and mTORC1 levels, and mTORC1 affects children's S6K1 and 4EBP1 levels."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2025
D-pdf
UI - Disertasi Membership  Universitas Indonesia Library
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Coppola, Gary M.
New York: John Wiley & Sons, 1987
547.2 COP a
Buku Teks  Universitas Indonesia Library
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Arif Kurniawan
"Latar Belakang: Sel kanker membutuhkan asam amino untuk metabolismenya telah ditemukan pada beberapa studi. Metabolisme ini meyebabkan profil asam amino mengalami perubahan pada kanker payudara. Profil asam amino pada kanker payudara ini dapat menentukan prognosis penyakit pada kanker payudara.
Tujuan: Mengetahui hubungan profil asam amino dengan faktor risiko kanker payudara di RS Ciptomangunkusumo, Indonesia.
Metode: Penelitian ini merupakan penelitian cross-sectional pada 19 subjek kanker payudara dan 19 subjek kontrol yang memenuhi kriteria penelitian di RSCM, Jakarta, Indonesia. Asam amino dianalisis menggunakan teknik liquid chromatography. Data tersebut dianalisis menggunakan uji independent sample t-test dan uji Mann-Whitney U.
Hasil:
Perbedaan profil asam amino pada kanker payudara yang signifikan dibandingkan dengan kontrol ditemukan pada peningkatan asam amino sistin dan penurunan asam amino valin, lisin, histidin, alanin, ornitin, tirosin, glutamin, fenilalanin, dan asam amino prolin. Terdapat signifikansi antara asam amino dengan faktor risiko usia, jumlah paritas, riwayat pemberian ASI, usia menarke, dan kebiasaan berolahraga.

Background: Cancer cell needs amino acids for its metabolism has been found in recent studies. Amino acids profile changes due to cancer cells’ metabolism. This amino acids profile in breast cancer could determine the prognosis of disease in breast cancer.
Objective: To aimed relationship between amino acids profile in breast cancer with its risk factor in Ciptomangunkusumo Hospital, Indonesia.
Method: A cross-sectional study in 19 breast cancer subjects and 19 control subjects that included into the criteria was conducted in this research. Amino acids was examined with liquid chromatography technique. The data was analyzed with independent sample t-test and Mann-Whitney U test.
Result: There are significant differences of amino acids profile between breast cancer subjects and control subjects, essentially for increased cystine profile and decreased valine, lysine, histidine, alanine, ornithine, tyrosine, glutamine, phenylalanine, and proline. There are also significance between amino acids profile in breast cancer with risk factors, including age, parity, breastfeeding history, menarche age, and workout habit.
"
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2021
T-pdf
UI - Tesis Membership  Universitas Indonesia Library
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Le Thandar Soe
"Penelitian ini bertujuan untuk mengetahui pengaruh dari optimized food-based recommendation FBR dan biskuit fortifikasi terhadap performa kognitif siswa sekolah dasar. Cluster randomized controlled trial selama enam bulan pada anak sekolah usia 7-9 tahun n=252 dilaksanakan di 12 sekolah di Kota Nyaundon yang terdiri dari 3 kelompok; kombimasi optimized FBR dan biskuit fortifikasi, optimized FBR, dan kontrol. Performa kognitif, antropometri, dan indicator biokimia diukur sebagai outcome. Pada kedua kelompok intervensi optimized FBR dengan biscuit fortifikasi dan optimized FBR terdapat pengaruh yang significant terhadap performa kognitif, weight-for-age z-scores dan kadar besi serum. Akan tetapi kombinasi optimized FBR dengan biscuit fortifikasi menghasilkan skor performa kognitif yang lebih tinggi dibandingkan optimized FBR saja dengan skor yang lebih tinggi secara signifikan pada daya ingat 1.1 0.1: p-vale

The study aimed to determine the effect of optimized food-based recommendation FBR and fortified biscuits on cognitive performance of primary school children. A six-month cluster randomized controlled trial among 7-9 years old school children n=252 were conducted at 12 schools in Nyaungdon Township with three intervention groups; optimized FBR with fortified biscuits, optimized FBR, and control. The cognitive performances, anthropometry and biochemical indicators were assessed as outcomes. Analysis of covariance and multiple linear regression analysis were done. Both intervention optimized FBR with fortified biscuits and optimized FBR groups had significant effect on the cognitive performances, weight-for-age z-scores and serum iron status. But combined optimized FBR with fortified biscuits improved cognitive performances higher scores than optimized FBR alone with significantly higher in memory 1.1 0.1: p-vale."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2018
D-Pdf
UI - Disertasi Membership  Universitas Indonesia Library
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Lanny Lestiani Sumali
"ABSTRACT
This study was carried out to ascertain the effect of
essential amino acids supplementation in renal failure patients on
dialysis. 22 renal failure patients on dialysis without any signs
and symptoms of G.I. disorders, liver disease, uncontrolled
diabetic mellitus were recruited to . the study. 11 patients served
as subjects and received essential amino acids supplementation, 11
others served as control group who received placebo. The subjects
had undergone basic physical examination, biochemical assessment
(Hb, transferrin, ureum and creatinine) and dietary assessment at
the beginning and at the end of the study. The study cone 1 uded
that the essential amino acids supplementation given to renal
failure patients on dialysis has significantly increased the
transferrin value, this may suggest an improvement of the protein
status of the patients. The study a 1 so pointed out that the
increased in transferrin as an effect of the essential amino acids
supplementation didt:l.'t cause an extra burden to the kidneys.
Further investigations are needed to reconfirm this finding"
1993
T-Pdf
UI - Tesis Membership  Universitas Indonesia Library
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Annisa Azhari
"Protein biomassa mikroba diharapkan menjadi sumber protein tambahan besar selama krisis pangan Limbah lumpur aktif telah dilaporkan mengandung nutrisi penting dan potensial sebagai pengganti protein non konvensional Protein terdiri dari asam amino dengan mengetahui cara efisien mengekstrak asam amino dari limbah lumpur aktif sangat penting untuk meningkatkan pemahaman produksi protein intraseluler dari air limbah Terdapat tiga metode yang diusulkan untuk mengevaluasi ekstrak asam amino dan asam amino bebas dari dua sumber lumpur limbah sintetis dan asli Metode 1 menggunakan ultrasonication untuk solubilisasi dan 32 wt HCl untuk 16 jam hidrolisis Metode 2 dibedakan menjadi dua metode solubilisasi a Alkali ultrasonication dan b Hanya alkali dilanjutkan dengan 6N HCl untuk 24 jam hidrolisis Metode 1 menghasilkan sedikit lebih tinggi dari ekstraksi total asam amino dengan total konten asam amino g g MLVSS 28 6 dan 24 0 dibandingkan dengan Metode 2a dengan 29 3 dan 21 6 untuk limbah sintetis dan limbah asli masing masingnya Secara signifikan Metode 2b memberikan hasil terendah dengan 11 6 pada limbah asli Dalam kesimpulan umum untuk skala limbah asli Metode 1 memberikan hasil yang terbaik untuk ekstraksi asam amino.

Microbial biomass protein is expected to be the major supplementary protein source during the food crisis. Waste activated sludge (WAS) has been reported to contain important nutrients and is potential as non-conventional protein substitutes. Proteins are composed of amino acids, by knowing how to efficiently extract amino acids from WAS is crucial to improve the understanding of intracellular protein production from wastewater. Three methods were proposed to evaluate the amino acids and free amino acids extraction from two sludge sources; lab and full-scale sludge. Method 1 utilised ultrasonication for solubilisation and 32 wt% HCl for 16h hydrolysis. Method 2 differentiate to two solubilisation methods; a). Alkaline + ultrasonication and b). Alkaline only, followed by 24h hydrolysis with 6N HCl. Method 1 gave slightly higher result of total amino acids extraction with a total amino acids content (g/g MLVSS) 28.6% and 24.0% compared to Method 2a with 29.3% and 21.6%, for lab scale and full scale sludge, respectively. Significantly, Method 2b gave lowest result with 11.6% in full scale sludge. In general conclusion for full scale sludge, Method 1 gave the best result for amino acids extraction.
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Depok: Fakultas Teknik Universitas Indonesia, 2015
S60810
UI - Skripsi Membership  Universitas Indonesia Library
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Irsan Hasan
"Background: The determination of branched chain amino acids (BCAA) to tyrosine ratio (BTR) was available in making differentiation of chronic hepatitis from liver cirrhosis, because there was a strong association between BTR and staging (fibrosis) scores. Branched chain ammo acids to tvrosine ratio have a correlation with Fischer ratio and the examination is easier because it can be done by enzymatic assay.
Materials and Methods: To evaluate the correlation between BTR and Child-Pugh score, we examined the amino gram of 52 liver cirrhosis patients consisted of 26 Child-Pugh A, 19 Child-Pugh B, and 7 Child-Pugh C. The examination of amino gram was done by High Pressure Liquid Chromatograph (HPLC) analyzer. Branched chain amino acids to tyrosine ratio were compared to Child-Pugh score, albumin, ammonia level, number connection test to Fischer ratio.
Results: Significant differences in BTR among Child-Pugh A, B, C were observed (Child-Pugh A 7.75 ± 1.2; Child Pugh B 6.0 ± 1.23 and Child Pugh C 4.38 + 3.14 (p = 0.000)). Branched chain amino acids to tyrosine ratio had a weak correlation with albumin fr - 0.292; p = 0.036), ammonia level (r = 0.376; p = 0.006) and strong correlation with Fischer ratio (r = 0.818; p = 0.000). There was no significant correlation between BTR and number connection test.
Conclusion: These results showed that the determination of the molar ratio of branched chain amino acids to tyrosine well reflected the severity of liver cirrhosis and it can be used as a substitute of Fischer ratio."
2005
IJGH-6-1-April2005-1
Artikel Jurnal  Universitas Indonesia Library
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