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"Tujuan penelitian ini adalah membandingkan hasil pengobatan adenomiosis dengan reseksi dan pemberian inhibitor aromatase. Kasus adenomiosis dengan infertilitas dikumpulkan selama 3 tahuti (Januari 1999 sampai December 2001) yang ilikonfirmasi dengan USG transvaginal. Kasus dibagi 2 kelompuk, masing-masing kelompok I (dengan reseksi per laparotomi) dan kelompok 2 (mendapat inhibitor aromatase anastrozole). Keduanya dinilai tentang gejala klinik, angka kehamilan. dan angka perkainbuhan pascaoperasi. Selama 3 tahun telah ditangani 1619 kasus infertilitas, di antaranya 66 (4.07%) adenomiosis sebanyak 55 kasus dianalisis, terdiri alas 32 kasus kelompok I dan 23 kasus kelompok 2. Dan 32 kasus yang menjalani reseksi, hasil histopatologik menunjukkan 30 (93.75%) adenomiosis dan 2 (6.25%) mioma uteri. Dalatn kelompok 1, 3 kasus hamil, 2 melahirkan bay! hiditp, I kasus teraklnr dengan abortus pada kehamilan 6 minggu. Sebanyak 25 kasus (78.1%) tidak hamil,4 kasus (12.5%) mengalami perkainbuhan, dan pada 24 kasus (75.35%) gejala-gejala klinis hilang. Sementara itu, 23 kasus kelompok 2, sebanyak 2 (8.6%) hamil, masing-masing 1 lahir hidup dan I abortus. Sebanyak 14 kasus (59.1%) gejala klinik hilang. Selama pengobatan 3 bulan dengan inhibitor aromatase terjadi penunuum ukuran lesi antara 7.31 mm' dan 25.90 mm dengan Cl 95% (p < 0.001). Disimpulkan bahwa pengobatan dengan inhibitor aromatase tidak menyembuhkan lesi, hanya mengurangi ukuran lest adenomiosis. Sebaliknya reseksi dapat menghilangkan lesi walauptin perkainbuhan dapat terjadi (12.5%) sesudah I tahun pascaoperasi. (MedJ Indones 2006; 15:18-23).

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The objective of this study was to observe the results of adenomyosis management with resection and administration of aromatase inhibitor. Cases ofademyosis in infertile women were collected for rhree years (January 1999 to December 2001) and the diagnoses were confirmed using transvaginal USG. Cases were grouped into two groups, i.e. group 1 (undergoing laparotomic resection) and group 2 (receiving treatment with aromatase inhibitor of anastrozole). Both groups were evaluated for changes in clinical symptoms, rate of successful pregnancy, and postoperative recurrency rate. During three years as many as 1619 infertility cases were managed, and among which 66 (4.07%) cases of adenomyosis were diagnosed with transvaginal USG. As many as 55 cases were analyzed, i.e., 32 cases underwent resection and 23 cases received aromatase inhibitor. Of 32 cases of surgical resection, the histopathologica! results showed 30 (93.75%) cases of adenomyosis and 2 (6.25%) cases of uterus myoma. In the group undergoing resection three cases (9.4%) were successfully pregnant, i.e., two cases had live birth, one case ended up with 6-week abortion. Moreover. 25 (78.1%) cases were not pregnant and 4 (12.5%) cases had recurrency, while 24 (75.35%) cases experienced disappearance of symptoms yet not pregnant. On the other hand, of 23 canes in the group receiving aromatase inhibitor 2 (8,6%) cases were able to be pregnant, one case had live birth and another case ended up with abortion, while 14 (59.1%) cases had disappearance of symptoms yet not pregnant. During three months of treatment with aromatase inhibitor, a reduction in the lesion size between 7.3! mm and 25.90 mm' were observed with Cl 95% (p < 0.001). In conclusion, treatment with aromatase inihibitor did not heal lesions, but only reduced the size of adenomyosis lesions. On the other hand, resection could heat lesions, yet recurrency of disease may occur (12.5%) after one postoperative year. (Med J Indones 2006; 15:18-23)."

"ABSTRAKTesis ini membahas kadar Anti Mullerian Hormone (AMH) sebagai salah satu prediktor cadangan ovarium dalam infertilitas. Tujuan penelitian adalah membandingkan kadar AMH perempuan berusia 40 tahun ke atas yang dapat hamil dengan perempuan berusia 40 taun ke atas yang mengalami infertilitas. Selain itu, melalui penelitian ini diharapkan juga diperoleh suatu nilai kadar AMH yang dapat digunakan untuk prediktor terjadinya kehamilan pada perempuan 40 tahun ke atas. Penelitian ini merupakan suatu studi potong lintang. Dari hasil studi ini, didapatkan bahwa tidak ada perbedaan signifikan kadar AMH pada perempuan usia 40 tahun ke atas yang mengalami kehamilan spontan dan dengan infertilitas. Di samping itu, penelitian ini belum bisa membuktikan peran kadar AMH untuk memprediksi terjadinya kehamilan pada perempuan usia 40 tahun ke atas.

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ABSTRACTThis thesis discusses the levels of Anti Mullerian Hormone (AMH) as a predictor of ovarian reserve in infertility. The research objective was to compare the levels of AMH women aged 40 years and over who can get pregnant naturally and in women aged 40 and over who are experiencing infertility. In addition, through this study is expected to also expect to have a value of AMH levels to be used for predictors of pregnancy in women 40 years and over. This study is a cross-sectional study. From the results of this study. From the results of this study, it was found that there was no significant difference in the levels of AMH in women aged 40 years and over who experienced spontaneous pregnancy and infertility. In addition, this study can’t prove a role for AMH levels predict the occurrence of pregnancy in women aged 40 years and over."

"Latar belakang: Infertilitas merupakan kegagalan mencapai kehamilan setelah 12 bulan atau lebih hubungan seksual teratur tanpa kontrasepsi. Secara global diperkirakan terdapat 48 juta pasangan dan 186 juta orang mengalami infertilitas yang juga dialami 10-15% pasangan usia reproduktif di Indonesia. Infertilitas dapat bersifat primer dan sekunder yang disebabkan oleh fakor perempuan, laki-laki, dan idiopatik. Pada perempuan, infertilitas dapat disebabkan oleh berbagai kelainan ovarium, uterus, saluran tuba, dan sistem endokrin. Histeroskopi merupakan baku emas evaluasi uterus yang dapat mendeteksi kelainan uterus yang mengganggu proses implantasi dan kehamilan serta mengevaluasi manfaat modalitas terapi dalam memperbaiki endometrium. Penelitian ini bertujuan mengidentifikasi peran dan temuan histeroskopi office pada diagnosis faktor uterus kasus infertilitas. Metode: Penelitian ini menggunakan studi cross sectional dengan metode deskriptif kuantitatif. Data yang digunakan adalah data sekunder rekam medis pasien RSCM Kintani pada kurun waktu 1 Juli 2020−30 Juni 2022. Sampel dipilih menggunakan metode consecutive sampling, diinput dan ditabulasi dalam bentuk tabel pada aplikasi Microsoft Excel yang kemudian disajikan dalam bentuk tabel, diagram, dan narasi deskriptif. Seluruh data dianalisis dengan analisis univariat deskriptif. Hasil: Temuan faktor uterus menggunakan histeroskopi office sebanyak 148 temuan terdiri dari 132 (89,19%) temuan abnormal dan 16 (10,81%) temuan normal uterus. Temuan abnormalitas yang diidentifikasi melalui histeroskopi office pada kasus infertilitas mencakup temuan mioma uteri sebanyak 12 (9,09%) temuan, adhesi (sinekia intrauterin) sebanyak 9 (6,82%) temuan, polip endometrium sebanyak 46 (34,85%) temuan, polip serviks sebanyak 17 (12,88%) temuan, stenosis OUI sebanyak 13 (9,85%) temuan, kelainan kongenital sebanyak 5 (3,79%) temuan, endometritis sebanyak 9 (6,82%) temuan, malignansi sebanyak 7 (5,30%) temuan, dan hiperplasia endometrium sebanyak 14 (10.61%) temuan. Kesimpulan: Temuan faktor uterus menggunakan histeroskopi office pada kasus infertilitas adalah 132 (89,19%) abnormal dan 16 (10,81%) normal. Dari 132 kasus abnormal, kasus terbanyak ialah polip endometrium 46 (34,85%), kemudian polip serviks sebanyak 17 (12,88%), hiperplasia endometrium sebanyak 14 (10,61%), stenosis OUI sebanyak 13 (9,85%), mioma uteri sebanyak 12 (9,09%), endometritis sebanyak 9 (6,82%), adhesi (sinekia intrauterine) sebanyak 9 (6,82%), malignansi sebanyak 7 (5,30%), dan kelainan kongenital sebanyak 5 (3,79%).

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ntroduction: Infertility is the failure to achieve pregnancy after 12 months or more of regular sexual intercourse without contraception. Globally, estimates suggest 48 million couples and 186 million individuals live with this problem. Infertility experienced by 10-15% of couples of reproductive age in Indonesia. Hysteroscopy is the gold standard for evaluating the uterine. Hysteroscopy has a role in infertility examination, namely detecting uterine abnormalities that can interfere with the implantation process, pregnancy, and also evaluating the benefits of therapeutic modalities in repairing the endometrium. This study aims to identify the role and findings of hysteroscopy office for diagnosis of uterine factors in infertility case. Method: This study was conducted using cross-sectional study with quantitative descriptive methods. The data used is secondary data from medical records of RSCM Kintani patients in the period July 1, 2020−June 30, 2022. The sample was selected using the consecutive sampling method, inputted and tabulated in tabular form in the Microsoft Excel application which was then presented in the form of tables, diagrams, and descriptive narratives. The data was analyzed by descriptive univariate analysis. Result: Uterine factor findings using office hysteroscopy totaled 148 findings consisting of 132 (89,19%) abnormal findings and 16 (10,81%) normal findings. Abnormal findings identified through office hysteroscopy in infertility cases included findings of uterine myoma in 12 (9,09%) findings, adhesions (intrauterine synechiae) in 9 (6,82%) findings, endometrial polyps in 46 (34,85%) findings, cervical polyps 17 (12,88%) findings, OUI stenosis 13 (9,85%) findings, congenital abnormalities 5 (3,79%) findings, endometritis 9 (6.82%) findings , malignancy in 7 (5,30%) findings, and endometrial hyperplasia in 14 (10,61%) findings. Conclusion: Uterine factor findings using office hysteroscopy in infertility cases were 132 (89.19%) abnormal and 16 (10,81%) normal. Of the 132 abnormal cases, the most common was endometrial polyps 46 (34,85%), then cervical polyps in 17 (12,88%), endometrial hyperplasia in 14 (10,61%), OUI stenosis in 13 (9,85% ), uterine myoma in 12 (9,09%), endometritis in 9 (6.82%), adhesions (intrauterine synechiae) in 9 (6,82%), malignancy in 7 (5.30%), and congenital abnormalities in 5 (3,79%)."

"Latar belakang: Fertilisasi in vitro (FIV) merupakan salah satu tata laksana utama dalam penanganan infertilitas. Penyuntikan human Chorionic Gonadotropin (hCG)eksogen merupakan salah satu tahapan penting dalam proses FIV untuk proses maturasi oosit. Walaupun sudah terdapat penelitian sebelumnya mengenai korelasi kedua hal tersebut, namun belum didapatkan suatu model prediksi maturitas oosit. Tujuan: Mengetahui korelasi kadar serum hCG 12 jam pascapenyuntikan terhadap tingkat maturitas oosit pada FIV dan model prediksi maturitas oosit. Metode: Penelitian ini merupakan sebuah penelitian potong lintang yang dilakukan pada peserta program FIV di Klinik Yasmin, RSUPN dr. Cipto Mangunkusumo, Jakarta, Indonesia sejak Januari 2020 hingga Desember 2020. Pasien dengan riwayat prosedur pembedahan ovarium, kemoterapi, radioterapi, dan peserta poor responder dieksklusi dari penelitian. Dilakukan penyuntikan r-hCG 250 µg secara subkutan pada semua subjek. Kadar serum hCG 12 jam pascapenyuntikan dan tingkat maturitas oosit setiap subjek dikumpulkan dan dianalisis. Hasil: Didapatkan sebanyak 28 subjek yang diikutsertakan dalam penelitian. Didapatkan korelasi yang tidak bermakna antara kadar hCG 12 jam pascapenyuntikan dan tingkat maturitas oosit (r = 0,052, p = 0,788). Namun, didapatkan kadar serum hCG 12 jam pascapenyuntikan yang lebih tinggi pada subjek dengan tingkat maturitas >75% (mean diff 34.78,p = 0.046). Didapatkan titik potong kadar serum hCG 12 jam pascapenyuntikan sebesar 90.15 mIU/mL untuk memprediksi tingkat maturitas yang baik. (sensitivitas 68.2%, spesifisitas 83.3%). Prediksi tingkat maturitas oosit dapat dilakukan dengan mengetahui kadar serum hCG 12 jam pascapenyuntikan dan indeks massa tubuh (IMT) subjek (sensitivitas 83.3%, spesifisitas 68.2%). Simpulan: Kadar serum hCG 12 jam pascapenyuntikan yang lebih tinggi berhubungan dengan tingkat maturitas oosit yang lebih baik pada peserta program FIV. Tingkat maturitas oosit dapat diprediksi melalui kadar serum hCG 12 jam pascapenyuntikan dan IMT.

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Background: In vitro fertilization (IVF) is one of the main treatments of infertility. Exogenous Human chorionic gonadotropin (hCG) injection is an important process of IVF and thought to be vital in determining oocyte maturation.

Purpose: This study aims to determine the relationship between 12 hours post-injection serum hCG and oocyte maturation rate on IVF participants.

Method: This is a cross-sectional study on IVF participants on Yasmin Clinic, dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia, during the period of January 2020 to December 2020. Subjects with history of ovarian surgery, chemotherapy, radiotherapy, and poor responder subjects were excluded from the study. Subjects were injected with 250 µg of r-hCG subcutaneously. Twelve hours post-injection serum hCG level and oocyte maturation rate were collected and analyzed accordingly.

Result: A total of 28 subjects were included in the study. It was found that higher 12 hours post-injection serum hCG was related with subjects with >75% oocyte maturation rate (mean diff 23.78, p = 0.046). The cut-off point of 12 hours post-injection serum hCG in order to predict better oocyte maturation rate was found to be 90.15 mIU/mL (sensitivity 68.2%, specificity 83.3%). Oocyte maturation rate predicted may be calculated using body mass index and 12 hours post-injection serum hCG. (sensitivity 83,3%, specificity 68,2%).

Conclusion: Higher 12 hours post-injection serum hCG was associated with higher oocyte maturation rate on IVF subjects. Oocyte maturation rate may be predicted using body mass index and 12 hours post-injection serum hCG."

"ABSTRAKEndometriosis menjadi salah satu penyebab infertilitas terbanyak pada perempuan. Penelitian ini bertujuan untuk menilai hubungan antara beberapa faktor risiko terhadap terjadinya endometriosis pada perempuan infertil. Studi kasus kontrol dilakukan pada 960 perempuan infertil di klinik Morula IVF Jakarta antara Januari 2015 sampai Desember 2016. Dari jumlah tersebut, diantaranya 320 perempuan infertil dengan endometriosis (kasus) dan 640 perempun infertil tanpa endometriosis (kontrol). Uji chi square digunakan untuk membandingkan kedua kelompok. Regresi logistik digunakan untuk membangun model prediksi endometriosis. Model regresi logistik menunjukkan bahwa dismenore dan indeks massa tubuh berhubungan secara signifikan dengan endometriosis. Faktor yang paling berhubungan dengan endometriosis adalah dismenore. Perempuan yang mengalami dismenore akan berpeluang 2,4 kali untuk mengalami endometriosis dibandingkan perempuan tanpa mengalami dismenore.Endometriosis is one of the most causes of female infertil.

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ABSTRACTThis study aims to assess the association between some of risk factors of endometriosis on infertile women. The case-control study was implemented on 960 infertile women at In Vitro Fertilization (IVF) Morula IVF Jakarta between January 2015 and December 2016. Of these, there were 320 infertile women with endometriosis (cases) and 640 infertile women without endometriosis (comparison group). Chi square test was used to compare the two groups. Logistic regression was used to build prediction model for an endometriosis diagnosis. In the logistic regression model contraception and body mass index were significantly associated with endometriosis. The most factors associated with endometriosis is dysmenorrhea. The women with dysmenorrhea will be 2.4 times higher risk in endometriosis than the women without dysmenorrhea."

"Latar belakang: Infertilitas dapat berasal dari pihak perempuan maupun laki-laki, termasuk di antaranya akibat jumlah spermatozoa yang kurang (oligozoospermia) ataupun gabungan dari gangguan pada jumlah, motilitas, dan morfologi spermatozoa (oligoastenoteratozoospermia/ OAT). Infertilitas sendiri biasanya dapat dideteksi menggunakan analisis semen konvensional, namun ternyata didapatkan bahwa 15% laki-laki yang infertil memiliki hasil analisis semen yang normal, sehingga perlu pula dilakukan analisis fragmentasi DNA dan maturasi kromatin spermatozoa untuk mengetahui kualitas spermatozoa lebih lanjut. Metode: Penelitian bersifat cross sectional, dilakukan terhadap 34 sampel (15 sampel oligozoospermia, 10 sampel OAT, dan 9 sampel fertil normozoospermia) yang diperoleh dari pasien dan petugas Klinik Infertilitas Yasmin Rumah Sakit Pusat Nasional Cipto Mangunkusumo (RSCM) Jakarta. Sampel kemudian dianalisis menggunakan SpermFunc® DNA-f kit untuk mengetahui indeks fragmentasi DNA (IFD)-nya serta SpermFunc® Histone kit untuk tingkat maturasi kromatinnya.Hasil: Untuk IFD spermatozoa, hasil uji ANOVA didapatkan bermakna (p: 0,003), dengan uji Post Hoc menunjukkan kelompok yang berbeda secara bermakna yaitu IFD OAT dan fertil (p: 0,003) serta IFD oligozoospermia dan OAT (p: 0,021). Sementara itu, uji Kruskal-Wallis menunjukkan perbandingan antara tingkat maturasi spermatozoa pada kelompok infertil dan fertil yang tidak bermakna (p: 0,289). Korelasi antara IFD maupun tingkat maturasi kromatin spermatozoa pada ketiga kelompok sangat lemah juga tidak bermakna, sehingga dapat diabaikan (r: -0,014; p: 0,936). Kesimpulan: Terdapat hubungan yang bermakna antara IFD OAT dibandingkan dengan kelompok fertil normozoospermia, namun sebaliknya pada hubungan IFD oligozoospermia dengan kelompok fertil. Adapun perbandingan tingkat maturasi kromatin spermatozoa kelompok infertil oligozoospermia dan OAT dengan kelompok fertil serta korelasi antara IFD dan tingkat maturasi kromatin spermatozoa pada kelompok yang diujicobakan bersifat tidak signifikan.

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Introduction: Infertility can be attributed to both female and male factors, included in male infertility causes are decreased sperm number (oligoozoospermia) as well as combination of defect in sperm quantity, motility, and morphology (oligoasthenoteratozoospermia/OAT). Male infertility usually can be detected through conventional semen analysis, however it is known that 15% of infertile males have normal semen analysis result, therefore it has become essential to do sperm DNA fragmentation and chromatin maturation analysis to know more about sperm quality. Method: This is a cross sectional study done to 34 samples (15 oligozoospermic samples, 10 OAT samples, and 9 fertile normozoospermic samples) that were collected from patients and staff of Yasmin Infertility Clinic at Rumah Sakit Pusat Nasional Cipto Mangunkusumo (RSCM) Kencana Jakarta. Those samples were then analyzed using SpermFunc® DNA-f kit to measure its DNA fragmentation index (DFI) and also using SpermFunc® Histone kit to measure its chromatin maturation percentage.Result: For sperm DFI, ANOVA test showed significance (p: 0,003), in which Post Hoc test confirmed that the groups with significancy in difference were the DFI of OAT and fertile group (p: 0,003) as well as oligozoospermic and OAT group (p: 0,021). On the other hand, Kruskal-Wallis test showed no signficance in the difference of sperm chromatin maturation percentage between infertile and fertile group (p: 0,289). The correlation between DFI and sperm chromatin maturation percentage of those groups was very weak and insignificant, thus negligible (Pearson correlation coeficient: -0,014; p value: 0,936). Conclusion: There is a significant relationship between the DFI difference of OAT and fertile normozoospermic group, but not between the DFI difference of oligozoopsermic and fertile group. On the other hand, sperm chromatin maturation difference between infertile oligozoospermic and OAT group and fertile group as well as the correlation of DFI and sperm chromatin maturation percentage on the groups that are being observed are not significant."

"Tujuan : Membandingkan dan menentukan perbedaan kadar homosistein dalam darah dan zalir folikel pada wanita infertil dengan dan tanpa endometriosis, kemudian menganalisis pengaruh kadar homosistein tersebut dengan mutu oosit.Metode : Penelitian ini merupakan penelitian potong lintang (cross sectional). Lima puluh sembilan subjek mengikuti program fertilisasi in-vitro yang masuk dalam kriteria penerimaan dibagi menjadi dua kelompok sama besar, yaknikelompok endometriosis dan tanpa endometriosis secara konsekutif (consecutive sampling). Masing-masing subjek diambil percontoh dari darah dan zalir folikel kemudian diukur kadar homosisteinnya dengan metode teraimun CMIA. Rerata masing-masing kelompok diuji statistik dengan uji t independen.Hasil : Rerata kadar homosistein dalam darah pada kelompok endometriosis lebih tinggi dibandingkan dengan tanpa endometriosis dan secara statisik berbeda bermakna (8,34 ±2,68 vs 6,71 ±1,56, p=0.007;95%CI: 0,02417-0,14657).Demikian pula dengan kadar homosistein dalam zalir folikel, kelompok endometriosis lebih tinggi dan secara statistik berbeda bermakna (6,19 ±1,67 vs 3,46 ±1,03; p= 0,000; 95% CI : 0,19310-0,32353). Semua mutu oosit baik padakedua kelompok, yakni derajat 3. Terdapat korelasi antara kadar homosistein di dalam darah dan zalir folikel pada kelompok endometriosis dan dinilai dengan uji Pearson didapatkan bermakna (p = 0,002) dan nilai korelasi 0,553 (kekuatan korelasi sedang) dan arah korelasi positif.Kesimpulan : Rerata kadar homosistein dalam darah dan zalir folikel pada wanita infertil dengan endometriosis lebih tinggi dibandingkan dengan tanpa endometriosis dan secara statistik berbeda bermakna. Kadar homosistein ini tidakberpengaruh terhadap mutu oosit. Terdapat korelasi positif antara kadar homosistein dalam darah dan zalir folikel pada kelompok endometriosis.

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Purpose : Compare and determine the differences in levels of homocysteine in

the blood and follicular fluid in infertile women with and without endometriosis, then analyze the effect of homocysteine levels with oocyte quality.

Method : This study was cross-sectional study. Fifty-nine subjects following the

in-vitro fertilization program are included in the admission criteria were divided into two equal groups, ie groups of endometriosis and without endometriosis consecutively (consecutive sampling). Each subject taken from the blood and follicular fluid then measured the levels of homocystein levels with immuoassay

method : The mean of each group was statistically tested with an independent t

test.

Results : The mean levels of homocysteine in the blood is higher in the endometriosis group than without endometriosis group and it was statisticaly significance (8,34 ±2,68 vs 6,71 ±1,56, p=0.007;95%CI: 0,02417-0,14657).

Similarly, the levels of homocysteine in follicular fluid , the endometriosis group

is higher and statisticaly significance (6,19 ±1,67 vs 3,46 ±1,03; p= 0,000; 95% CI : 0,19310-0,32353). All oocytes are in good quality in both groups, maturation grade 3. There is a correlation between the levels of homocysteine in the blood and follicular fluid in the endometriosis group and assessed with Pearson test, and it found significant (p = 0.002) and the correlation value 0.553 (moderate correlation strength) and direction of a positive correlation.

Conclusion : The mean levels of homocysteine in the blood and follicular fluid in infertile women with endometriosis is higher than without endometriosis and were statistically significantly different. These homocysteine levels does not affect the quality of oocytes. There is a positive correlation between the levels of homocysteine in the blood and follicular fluid in endometriosis group."

"Infertilitas terjadi pada 10-15 pasangan di dunia. Faktor laki-laki terjadi pada 35 kasus infertilitas dan 10-20 terjadi karena keduanya. Salah satu uji untuk mendeteksi faktor infertilitas pada laki-laki adalah dengan uji fungsional spermatozoa yaitu uji integritas membran dan fragmentasi DNA spermatozoa. Uji integritas membran spermatozoa adalah uji untuk melihat keutuhan dan fungsi dari membran plasma spermatozoa. Uji fragmentasi DNA spermatozoa adalah uji untuk melihat keutuhan dari DNA spermatozoa. Penelitian ini ingin mengetahui hubungan antara integritas membran dengan fragmentasi DNA spermatozoa. Penelitian ini menggunakan metode potong lintang dengan 100 sampel dari hasil laboratorium laki-laki infertil di Klinik Yasmin RSCM Kencana. Uji integritas membrane spermatozoa dilakukan dengan uji Hypo-osmotic Swelling HOS sedangkan fragmentasi DNA spermatozoa diuji dengan uji Sperm Chromatin Dispersion SCD. Uji dan pengelompokan analisis semen ini dilakukan berdasarkan WHO laboratory manual for examination and processing of human semen edisi ke-lima. Hasil dari penelitian ini menyatakan bahwa terdapat korelasi negatif yang signifikan p=0,008 dengan kekuatan korelasi lemah r=-0,265 antara integritas membran dengan fragmentasi DNA spermatozoa.

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Infertility occurs in 10 15 of couple in the world. Male factor occurs in 35 of infertility cases and 10 20 occurs as both. One of the test to detect infertility factor in men is the spermatozoa functional test whereas the spermatozoa membrane integrity and DNA fragmentation test. Spermatozoa membrane integrity test is a test to see the integrity and function of the plasma membrane of spermatozoa. Sperm DNA fragmentation test is a test to determine the integrity of the DNA of spermatozoa. This study investigates the relationship between the membrane integrity of spermatozoa with DNA fragmentation.

This study using cross sectional method with 100 samples of laboratory result in Klinik Yasmin Kencana RSCM. Spermatozoa membrane integrity test conducted by Hypo osmotic swelling test and spermatozoa DNA fragmentation tested with Sperm Chromatin Dispersion test. The semen analysis is based on WHO laboratory manual for examination and processing of human semen 5th edition. The result of this study is that there is a significant negative weak correlation p 0,008, r 0,265 between the spermatozoa membrane integrity and DNA fragmentation. "