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Hasil Pencarian

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Cindy Gisella Zahrany
"Tingginya insiden infeksi demam berdarah yang terjadi dan tidak adanya vaksin efektif menyebabkan banyak peneliti mencoba ekstrak tumbuhan sebagai pengobatan alternatif pada virus Dengue (DENV). Curcumin merupakan salah satu ekstrak tumbuhan yang telah dibuktikan memiliki efek antiviral. Penelitian ini bertujuan untuk mengetahui apakah curcumin memiliki efek antiviral pada virus dengue. Oleh karena itu dilakukan tes untuk mengetahui persen hambatan curcumin pada replikasi DENV dan efek cytotoxic curcumin pada sel mamalia. Penelitian ini merupakan penelitian eksperimental yang dilakukan di Departemen Mikrobiologi FKUI.
Pada penelitian ini terdapat enam kelompok yaitu perlakuan oleh curcumin dengan empat konsentrasi yang berbeda kontrol negatif dan juga Dimethil Sulfoxide (DMSO). Data yang didapatkan dari eksperimen ini akan dianalisis dengan metode T-test. Dari hasil penelitian terlihat bahwa curcumin terbukti dapat menghambat replikasi virus dengue. Pemberian dosis yang lebih tinggi dapat menghambat 100% replikasi virus. Pada saat konsentrasi curcumin diturunkan, maka penghambatan replikasi DENV secara dratis menurun. Dari data tersebut IC50 dari curcumin diperoleh yaitu kurang dari 0.1 µg/ml.
Hasil data menunjukkan bahwa efek cytotoxic curcumin pada sel sangat signifikan pada kosentrasi yang tinggi. Pada konsentrasi yang lebih rendah, viabilitas sel terhitung lebih tinggi. Dari data tersebut dapat dihitung nilai CC50 yaitu 3,46 µg/ml. Dengan membandingkan nilai CC50 dan IC50 dari curcumin, didapatkan nilai selectivity index yaitu lebih dari 34. Dari penelitian ini dapat disimpulkan bahwa curcumin dapat digunakan sebagai antiviral virus dengue di masa mendatang.

The high incidence of dengue virus infection and also the absence of effective vaccine cause researchers to look up to use the natural extract as the alternative remedy against the dengue virus (DENV). Curcumin is one of the natural extracts that has already proven to have antiviral effect. The objective of this study experiment aimed to see whether curcumin can be used as the antiviral against dengue virus. Several experiments were conducted to obtain the percentage of inhibition of DENV replication and also to determine the cytotoxic effect of curcumin to mammalian cells. This study was an experimental study that had been conducted at Microbiology Departement of Faculty Medicine of Universitas Indonesia.
In this experiment, there were six treatment groups such as four different concentrations of curcumin, negative control and Dimethyl sulfoxide (DMSO). The data from this study were analyzed using T-test method. From this study, the curcumin had been proven to successfully inhibit the replication of dengue virus. The treatment with higher dose of curcumin could totally inhibit the replication of DENV. When we gave less dose of curcumin, the percentage inhibition dropped significantly.
This showed that inhibition by curcumin was in dose-dependent manner. Furthermore, from these data we determined the IC50 of curcumin which was less than 0.1 µg/ml. The CC50 of curcumin was 3,46µg/ml. By comparing the result of CC50 and IC50, we found the selectivity index value was more than 34. From this study, it can be concluded that Curcumin can be used as antiviral against dengue virus in the future.
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Jakarta: Fakultas Kedokteran Universitas Indonesia, 2016
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UI - Skripsi Membership  Universitas Indonesia Library
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Agriana Puspitasari
"[ABSTRAK
Latar Belakang: Endometriosis diperkirakan ditemukan pada 2-22% wanita usia reproduksi yang asimptomatik, sedangkan pada wanita yang mengalami dismenore, prevalensinya meningkat menjadi 40-60%. Terapi yang ada saat ini adalah terapi medikamentosa, terapi pembedahan, atau gabungan dari keduanya. Namun belum ada yang dapat berhasil menghilangkan penyakit ini. Hal ini dibuktikan dengan angka kekambuhan endometriosis yang cukup tinggi, yaitu 33,3-40,3%. Pada penderita endometriosis, terjadi proses inflamasi akibat adanya stress oksidatif yang berasal dari perdarahan siklik. Pada perdarahan siklik ini didapatkan heme dan besi yang merupakan suatu oksidan. Beratnya stress oksidatif yang terjadi dapat dilihat dari kadar malondialdehida dalam darah karena radikal bebas yang merupakan bagian dari ROS akan mengubah asam lemak jenuh menjadi aldehid dan malondialdehida (MDA). Telah diketahui bahwa kadar MDA pada jaringan endometriosis lebih tinggi secara bermakna dibandingkan dengan endometrium eutopik. Kurkumin diketahui mempunyai efek antiinflamasi, antioksidan, dan imunomodulator. Efek antioksidan dari kurkumin bekerja dengan cara mengurangi jumlah radikal bebas yang beredar.
Tujuan: Menilai pengaruh pemberian kurkumin terhadap stress oksidatif pada penderita endometriosis.
Metode: Penelitian ini merupakan suatu penelitian uji klinis acak tersamar ganda dengan kontrol pasien yang mendapat kapsul plasebo selama periode Desember 2014 ? Mei 2015. Pengambilan sampel dilakukan dengan consecutive sampling.
Hasil: Sejumlah 12 subjek dari kelompok kurkumin diberikan perlakuan dengan 1x100 mg kurkumin selama 2 bulan, sedangkan 12 subjek pada kelompok kontrol diberikan kapsul plasebo selama 2 bulan, setelah sebelumnya diambil MDA pre perlakuan. Satu pasien dari kelompok kurkumin dan 2 dari kelompok kontrol drop-out karena tidak kembali pada akhir bulan kedua untuk pengambilan MDA pasca perlakuan. Rerata awal kadar MDA subjek kelompok plasebo adalah 0,39 ± 0,39 nmol/ml dengan rerata kadar MDA di akhir intervensi 0,32 ± 0,14 nmol/ml. Penurunan tersebut tidak bermakna berdasarkan uji statistik dengan nilai p=0,80. Rerata awal (baseline) kadar MDA subjek dengan suplementasi kurkumin adalah 0,33 ± 0,21 nmol/ml dengan rerata kadar MDA pasca intervensi berkurang menjadi 0,31 ± 0,13 nmol/ml. Secara statistik penurunan kadar MDA pasca suplementasi kurkumin tidak bermakna (p=0,84). Tidak didapatkan perbedaan bermakna kadar MDA awal antar kedua kelompok (p=0,56). Demikian juga pada kadar MDA akhir intervensi dan perubahan (delta) kadar MDA antar kedua kelompok setelah intervensi, tidak dijumpai berbedaan bermakna secara statistik dengan p=0,85 dan p=0,81, berturut-turut
Kesimpulan: Tidak terdapat penurunan kadar MDA yang bermakna pada subjek dengan suplementasi kurkumin maupun plasebo.

ABSTRACT
Background: Endometriosis is estimated to be found in 2-22% asymptomatic reproductive women, while women with dysmenorrhea, the prevalence increased to 40-60%. Current management is medical therapy, surgical therapy, or a combination of both. But no one has been able to successfully eliminate this disease. This is proven by endometriosis recurrence rate is high enough, ranging from 33.3 to 40.3%. In endometriosis, inflammatory process occurs as a result of oxidative stress originating from cyclic bleeding. At this cyclic bleeding obtained heme and iron which is an oxidant. Free radicals that are part of the ROS (reactive oxygen species) will change the saturated fatty acids to aldehydes and malondialdehydes (MDA), so oxidative stress that occurs can be seen from plasma malondialdehyde levels. In recent study, MDA levels in endometriosis tissue was significantly higher than the eutopic endometrium. Curcumin is known to have anti-inflammatory, antioxidant and immunomodulatory effects. Antioxidant effects of curcumin works by reducing the amount of circulating free radicals.
Objective: Assess the effect of curcumin on oxidative stress in endometriosis patients
Methods: This study is a randomized double-blind clinical trial with control groups receiving placebo capsules for the period December 2014 - May 2015. Sampling was conducted by consecutive sampling.
Results: Twelve subjects of the treatment group was given curcumin 1x100 mg, while 12 subjects in the control group was given placebo capsules for 2 months. Peripheral blood was taken for MDA levels pre treatment. One patient from curcumin group and 2 from the control group dropped out because they do not come at the end of treatment for MDA measurement. The mean initial MDA level of placebo group was 0.39 ± 0.39 nmol / ml with a mean MDA levels at the end of the intervention 0.32 ± 0.14 nmol / ml. The decrease was not statistically significants with p = 0.80. The mean initial MDA levels of curcumin group was 0.33 ± 0.21 nmol / ml with a mean at the end of intervention was 0.31 ± 0.13 nmol / ml. The decrease was not statistically significants with p = 0.84. There were no significant differences between the initial MDA levels both groups (p = 0.56). Likewise, at MDA levels post intervention and delta between the MDA pre and post intervention on both groups, found no statistically significant with p = 0.85 and p = 0.81, respectively.
Conclusions: There was no significant decrease in MDA levels in subjects with curcumin supplementation or placebo., Background: Endometriosis is estimated to be found in 2-22% asymptomatic reproductive
women, while women with dysmenorrhea, the prevalence increased to 40-60%. Current
management is medical therapy, surgical therapy, or a combination of both. But no one has
been able to successfully eliminate this disease. This is proven by endometriosis recurrence
rate is high enough, ranging from 33.3 to 40.3%. In endometriosis, inflammatory process
occurs as a result of oxidative stress originating from cyclic bleeding. At this cyclic bleeding
obtained heme and iron which is an oxidant. Free radicals that are part of the ROS (reactive
oxygen species) will change the saturated fatty acids to aldehydes and malondialdehydes
(MDA), so oxidative stress that occurs can be seen from plasma malondialdehyde levels. In
recent study, MDA levels in endometriosis tissue was significantly higher than the eutopic
endometrium. Curcumin is known to have anti-inflammatory, antioxidant and
immunomodulatory effects. Antioxidant effects of curcumin works by reducing the amount
of circulating free radicals.
Objective: Assess the effect of curcumin on oxidative stress in endometriosis patients
Methods: This study is a randomized double-blind clinical trial with control groups receiving
placebo capsules for the period December 2014 - May 2015. Sampling was conducted by
consecutive sampling.
Results: Twelve subjects of the treatment group was given curcumin 1x100 mg, while 12
subjects in the control group was given placebo capsules for 2 months. Peripheral blood was
taken for MDA levels pre treatment. One patient from curcumin group and 2 from the control
group dropped out because they do not come at the end of treatment for MDA measurement.
The mean initial MDA level of placebo group was 0.39 ± 0.39 nmol / ml with a mean MDA
levels at the end of the intervention 0.32 ± 0.14 nmol / ml. The decrease was not statistically
significants with p = 0.80. The mean initial MDA levels of curcumin group was 0.33 ± 0.21
nmol / ml with a mean at the end of intervention was 0.31 ± 0.13 nmol / ml. The decrease
was not statistically significants with p = 0.84. There were no significant differences between
the initial MDA levels both groups (p = 0.56). Likewise, at MDA levels post intervention and
delta between the MDA pre and post intervention on both groups, found no statistically
significant with p = 0.85 and p = 0.81, respectively.
Conclusions: There was no significant decrease in MDA levels in subjects with curcumin supplementation or placebo.]"
Fakultas Kedokteran Universitas Indonesia, 2015
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UI - Tugas Akhir  Universitas Indonesia Library
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Sumbung, Nielda Kezia
"Latar Belakang: Cisplatin merupakan salah satu obat kemoterapi yang biasa digunakan untuk mengobati berbagai jenis kanker. Namun, meskipun kemampuannya sangat baik dalam mengatasi kanker, cisplatin dapat menyebabkan nefrotoksisitas. Curcumin memiliki efek antioxidan dan anti inflamasi yang diperkirakan dapat melindungi ginjal dari toksisitas cisplatin. Namun, bioavailabilitas curcumin yang rendah menjadi perhatian utama. Pada percobaan ini, kami akan membandingkan efektivitas kurkumin dan nanokurkumin dalam hal
proteksi terhadap ginjal pada tikus yang diberikan cisplatin injeksi diperiksa menggunakan KIM-1 dan NGAL sebagai biomarker nefrotoksisitas akut. Metode: Tikus Sprague-dawley jantan dipilih secara acak dan dikelompokkan ke dalam 5 grup (n = 5 tikus/grup) dengan perlakuan yang berbeda; normal, cisplatin, cisplatin + curcumin, cisplatin + nanocurcumin 50 mg/kgBB, dan cisplatin + nanocurcumin 100 mg/kgBB. Dosis cisplatin yang digunakan sebesar 7 mg/kgBB. Pada hari ke 10, tikus dikorbankan dan ginjal diambil untuk dianalisis. Ekspresi KIM-1 dan NGAL pada ginjal dianalisa menggunakan RT-PCR. Hasil: Tidak ada perbedaan diantara seluruh kelompok (p>0.05). Namun, ekspresi kedua gen lebih rendah pada grup yang diberikan nanocurcumin. Konklusi: Ekspresi KIM-1 dan NGAL menurun setelah administrasi nanocurcumin, meskipun tidak signifikan.
Background: Cisplatin is one of the chemotherapy drugs that is commonly used to treat many kinds of cancer. However, despite its great effect, cisplatin can trigger nephrotoxicity due to its usage. Curcumin, has antioxidant and anti-inflammatory effect that has been suggested to be able to protect the kidney from cisplatin
toxicity. Nevertheless, its low bioavailability has become one of the major concern. In this experiment, we will compare the effectivity of curcumin and nanocurcumin in protecting the kidney from cisplatin-induced nephrotoxicity using KIM-1 and NGAL as the biomarker of acute kidney failure Method: Sprague Dawley rats are randomly divided into 5 groups (n = 5 rats/group) with different treatment; normal, cisplatin, cisplatin+curcumin, cisplatin+nanocurcumin 50 mg/kgBW, cisplatin+nanocurcumin 100mg/kgBW. The dose of cisplatin used in this research is 7mg/kgBW. On the 10th day of experiment, the rat is sacrified and the kidneys are taken for analysis. Then, KIM and NGAL expression in the kidney is analyzed using qRT-PCR. Results: There are no statistical significancy between all group (p>0.05). However,
expression of both KIM-1 and NGAL decrease in group treated using
Nanocurcumin Conculsion: The expression of both KIM-1 and NGAL are repressed by nanocurcumin, although it is statistically not significant."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2018
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UI - Skripsi Membership  Universitas Indonesia Library
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Clarissa Fidelia
"Tuberkulosis adalah penyebab kematian tertinggi ke-10 di dunia yang disebabkan oleh bakteri Mycobacterium tuberculosis dan umumnya menyerang paru-paru. Pasien tuberkulosis seringkali diharuskan untuk mengonsumsi sejumlah Obat Antituberkulosis (OAT), salah satunya rifampisin (RIF), dalam rentang waktu enam bulan. Degradasi RIF akibat paparan oksigen menyebabkan terbentuknya senyawa degradasi inaktif rifampisin kuinon (RIF-Q). OAT yang tidak stabil dapat menyebabkan pengobatan yang kurang efektif dan meningkatkan kemungkinan resistansi pasien terhadap obat tersebut. Dalam penelitian ini, dilakukan penambahan antioksidan curcumin, piperine, gingerol dan mangostin untuk mencegah oksidasi RIF. Hasil analisis instrumen LC-MS/MS terhadap sampel RIF yang berumur tiga bulan menunjukkan adanya produk degradasi inaktif dalam rasio dua kali lipat dibanding senyawa aktif. Adanya pengaruh penambahan antioksidan terhadap sampel RIF dianalisis dengan penentuan potensial oksidasi menggunakan metode cyclic voltammetry (CV) dan uji stabilitas selama enam hari menggunakan instrumen HPLC. Penambahan gingerol memberikan pengaruh paling signifikan terhadap kestabilan RIF dalam larutan PBS dibandingkan antioksidan lain, serta penambahan curcumin mampu menggeser potensial oksidasi OAT RIF ke arah yang lebih positif serta mempertahankan konsentrasi RIF dengan lebih optimal. Senyawa curcumin dan gingerol memiliki potensi untuk dijadikan kandidat sebagai agen penstabil RIF.

Tuberculosis is one of the top 10 cause of death worldwide which is caused by Mycobacterium tuberculosis infections, commonly occurs in lungs. TB patients are often prescribed with a few antitubercular drugs such as rifampicin (RIF) for six months period. Degradation of RIF caused by oxygen produce inactive product rifampicin quinone (RIF-Q). Unstable drugs may compromise treatment effectiveness and enhance the possibility of drug resistance in patient. Therefore, in this study natural antioxidant such as curcumin, piperine, gingerol and mangostin are added to prevent RIF oxidation. Analysis by LC-MS/MS on three-months-old RIF sample shows evidence of inactive degradation product twice the ratio of the active compound. Antioxidant addition influences on RIF are analyzed with determining oxidation potential utilizing cyclic voltammetry (CV) method and stability test for six days utilizing HPLC instrument. Addition of gingerol gives the most significant influence on the stability of RIF in PBS solution, while the addition of curcumin could shift oxidation potential of RIF to more positive value and optimally maintain RIF concentration compared to other antioxidants. Therefore, gingerol curcumin has the potential to be stabilizer agent for RIF drug."
Depok: Fakultas Teknik Universitas Indonesia, 2020
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UI - Skripsi Membership  Universitas Indonesia Library
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Candra Wijaya
"Tujuan: Membandingkan aktivitas angiogenik plasenta preeklampsia dengan dan tanpa pemberian kurkumin dan vitamin E.
Rancangan Penelitian: Penelitian ini merupakan studi eksperimental in vitro. Plasenta dari ibu hamil preeklampsia (n=11) dibagi dalam 3 kelompok: kelompok kontrol, kelompok pemberian kurkumin dosis 0,01 mM, dan kelompok pemberian vitamin E dosis 20 mg/L Aktivitas angiogenesis ditentukan dengan menilai skor migrasi sel-sel endotel menuju plasenta. Analisis perbedaan aktivitas angiogenesis antar kelompok digunakan tes wilcoxon.
Hasil: Aktivitas angiogenik kelompok pemberian kurkumin dosis 0,01 mM tidak berbeda bermakna dibandingkan kelompok kontrol (p>0,05). Sedangkan, aktivitas angiogenik kelompok pemberian vitamin E dosis 20 mg/L berbeda secara bermakna dibandingkan kelompok kontrol (p< 0,05).
Kesimpulan: Pemberian vitamin E meningkatkan aktivitas angiogenik pada plasenta dari ibu hamil preeklampsia.

Objective: To compare angiogenic activity in preeclamptic placenta with and without supplementation of curcumin and vitamin E.
Study design: The study was an in vitro experimental study. Placentae were obtained from woman with preeclampsia (n=11) divided into three groups. The first was control, to the second group 0,01 mM curcumin was added and the third with 20 mg/I, vitamin E. Angiogenic activity was assayed using an endothelial cell migration assay. Differences in placental angiogenic activity between three groups were analysed using the Wilcoxon test.
Results: The angiogenic activity in the 0,01 mM curcumin supplementation group was not significantly different than in the control group (p>0,05). While, angiogenic activity in the 20 mg/I, vitamin E group was significantly different than in the control group (p< 0,05).
Conclusion: Vitamin E supplementation increased angiogenic activity in the placenta from women with preeclampsia."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2006
T 17686
UI - Tesis Membership  Universitas Indonesia Library
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Gaviota Hartono
"Latar Belakang: Berbagai penelitian terdahulu telah membuktikan bahwa kurkumin memiliki sifat hepatoprotektif sehingga memungkinkannya untuk mengobati banyak jenis penyakit hepar. Meskipun kurkumin aman dan mempunyai banyak aktifitas biologis, penggunaan kurkumin belum dapat digunakan secara komersil sebagai obat terapeutik karena tingkat absorbsi, stabilitas, dan bioavailabilitas yang rendah serta metabolisme kurkumin yang cepat. Berhubung studi mengenai efek pengurangan ukuran partikel untuk meningkatkan distribusi jaringan belum dilakukan sepenuhnya, penelitian ini bertujuan untuk mengetahui apabila peningkatan konsentrasi kurkumin di jaringan hepar dapat dilakukan dengan menggunakan nanopartikel.Metode: Penelitian ini merupakan penelitian in vivo pada tikus. Tikus dirandomisasi menjadi 2 kelompok, masing-masing 5 ekor yang mendapatkan kurkumin konvensional 500 mg/kgBB atau nanokurkumin 500 mg/kgBB dosis tunggal secara oral. Sampel hati diambil setelah 3 atau 4 jam setelah pemberian obat dan konsentrasi kurkuminnya dikuantifikasi menggunakan UPLC-MS/MS.Hasil: Konsentrasi nanokurkumin lebih tinggi daripada konsentrasi kurkumin konvensional di jaringan hepar setelah 3 jam dan relatif lebih tinggi setelah 4 jam. Pada 3 jam, konsentrasi rerata nanokurumin (33.1934 ng/mg) adalah lebih dari 7 kali lipat dibandingkan konsentrasi rerata kurkumin (4.5189 ng/mg) dan bermakna secara statistik (p = 0.047). Pada 4 jam, konsentrasi rerata nanokurumin (11.8725 ng/mg) hanya sedikit lebih tinggi dibandingkan konsentrasi rerata kurkumin (11.6352 ng/mg) dan tidak bermakna secara statistik (p = 0.251).Konklusi: Pemberian nanokurkumin secara oral menghasilkan konsentrasi kurkumin yang lebih tinggi di hati tikus setelah 3 dan 4 jam daripada kurkumin konvensional.
Background: Many previous researches have proven that curcumin possesses potent hepatoprotective propertiy which enables it to treat and prevent the progression of different hepatic disorders. However, despite its superior safety profile and biological activity, curcumin has not been commercially used as a therapeutic drug due to its extremely poor absorption and stability, low bioavailability and rapid metabolism. As the effect of decreasing its particle size to improve its tissue distribution have yet to be studied thoroughly, this research aims to find out if higher curcumin concentrations in liver tissue can be achieved by using nanoparticles.Method: This research is an in vivo research in rats. The rats are randomized into 2 groups, each with 5 rats which were given either single doses of 500 mg/kgBW conventional curcumin or 500 mg/kgBW nanocurcumin orally. The liver samples were obtained after 3 or 4 hours, followed by curcumin concentration measurement using the UPLC-MS/MS method. Results: Nanocurcumin concentrations were higher than curcumin concentrations in the liver tissue at 3 hours and relatively higher at 4 hours. At three hours, the mean nanocurcumin concentration (33.1934 ng/mg) is over 7 times higher than mean curcumin concentration (4.5189 ng/mg) and is statistically significant (p = 0.047). At 4 hours, the mean nanocurcumin concentration (11.8725 ng/mg) is slightly higher than mean curcumin concentration (11.6352 ng/mg) not statistically significant (p = 0.251)Conclusion: Oral administration of nanocurcumin results in higher curcumin concentrations in rat liver tissue after 3 and 4 hours compared to conventional curcumin."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2018
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UI - Skripsi Membership  Universitas Indonesia Library
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Dianne Adha
"Latar Belakang : Tingginya prevalensi penyakit HIV/AIDS di Indonesia, tidak banyak penelitian tentang pengaruh bahan alam terhadap imunitas sehubungan dengan infeksi HIV, adanya fakta berbagai penelitian yang menyatakan kurkumin dapat mempengaruhi berbagai faktor transkripsi dan belum terdapat informasi mengenai pengaruh kurkumin terhadap ekspresi mRNA APOBEC3G , maka dilakukan penelitian ini untuk mengetahui dan menilai aktivitas kurkumin terhadap ekspresi mRNA APOBEC3G pada sel PBMC manusia.
Metode : Isolasi PBMC dari whole blood dan mengkultur sel PBMC dengan kurkumin konsentrasi 10uM, 20uM dan 50uM , diinkubasi selama 24 dan 48 jam. Kemudian dilakukan isolasi RNA pada setiap kelompok perlakuan, mengukur konsentrasi dan kemurnian RNA. Dilakukan analisis ekspresi mRNA APOBEC3G menggunakan real-time RT PCR, nilai Ct dari setiap perlakuan diolah menggunakan metode Livak sehingga diperoleh nilai tingkat ekspresi relatif APOBEC3G yang dipengaruhi kurkumin. Dilakukan elektroforesis gel dan sekuensing untuk memastikan primer yang digunakan spesifik untuk APOBEC3G dan GAPDH.
Hasil : Pada perlakuan kurkumin konsentrasi 10uM, 20uM dan 50uM yang diinkubasi 24 jam didapatkan jumlah sel pada masing-masing perlakuan konsentrasi kurkumin 8x105, 7,5x105 dan 7x105. Jumlah total RNA masing-masingnya 103,4; 101,1 dan 95,9. Tingkat ekspresi relatif mRNA APOBEC3G 0,66; 0,60 dan 0,49. Sedangkan pada inkubasi 48 jam jumlah sel pada masing-masing perlakuan konsentrasi kurkumin 6,5x105, 6x105 dan 5x105. Jumlah total RNA masing-masingnya 74,95; 69,75 dan 53,7. Tingkat ekspresi relatif mRNA APOBEC3G 0,59; 0,47 dan 0,40.
Kesimpulan : Semakin tinggi konsentrasi kurkumin dan semakin lama waktu inkubasi menunjukkan semakin berkurang jumlah sel, jumlah RNA total dan tingkat ekspresi relatif mRNA APOBEC3G.

Background : High prevalence of HIV/AIDS in Indonesia, there are not many research about effects of natural resources to immunity in context of HIV infection, though there are facts about research which states curcumin can affect various transcription factors and none about effect of curcumin on expression of mRNA APOBEC3G, therefore this research is conducted in order to obtain information and measure curcumin's activity on APOBEC3G mRNA expression in human PBMC cell.
Methods : PBMC isolation from whole blood specimen and PBMC culture with curcumin concentration of 10uM, 20uM, 50uM, incubated for 24 and 48 hours. After incubation RNA isolation procedure was then conducted on each group treatment followed by concentration and purity measurement of RNA. Expression analysis of APOBEC3G mRNA using real-time RT PCR was performed. Livak method was used to procces Ct results from each treatment which results in relatives expression levels of APOBEC3G effected by curcumin. Gel electrophoresis and sequencing was done to make sure that primers being used are specific for APOBEC3G and GAPDH.
Results : On treatment concentration of curcumin 10uM, 20uM and 50uM which has been incubated for 24 hours received results of curcumin concentration of 8x105, 7,5x105 and 7x105. Total rna results 103,4; 101,1 and 95,9. Relative expression levels of APOBEC3G mRNA 0,66; 0,60 and 0,49. 48 hour incubation received results of curcumin concentration of 6,5x105, 6x105 and 5x105. Total rna results 74,95; 69,75 and 53,7. Relative expression levels of APOBEC3G mRNA 0,59; 0,47 and 0,40.
Conclusion : Higher levels of curcumin concentration and longer incubation periods shows decreased cell count, total RNA number and relative expression level of mRNA APOBEC3G."
Depok: Universitas Indonesia, 2013
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UI - Tesis Membership  Universitas Indonesia Library
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Basalamah, Ahmad Muhammad
"Kanker ovarium merupakan kanker ginekologi tersering kedua, setelah kanker serviks dan juga merupakan salah satu jenis kanker dengan angka insidensi tertinggi pada wanita Indonesia. Cisplatin adalah salah satu jenis kemoterapi yang sering digunakan dalam terapi kanker ovarium. Cisplatin bekerja dengan memproduksi oksidatif stress untuk melawan sel kanker, akan tetapi mekanisme ini berpotensi untuk membahayakan tubuh, seperti menyebabkan toksisitas pada organ seperti hati dan ginjal, maupun toksisitas sistemik. Untuk mengurangi efek samping dari cisplatin, curcumin dapat ditambahkan. Curcumin juga dapat meningkatkan efek anti kanker cisplatin, akan tetapi bioavailabilitas curcumin masih cukup rendah. Oleh karena itu, kelompok penelitian kami mengembangkan curcumin dalam bentuk nano partikel, atau Nanocurcumin dengan ukuran 11,5 nm, yang sudah terbukti meningkatkan bioavailabilitas kurkurmin dalam tubuh. Penelitian ini adalah penelitian in vivo pada tikus Wistar betina. Penelitian ini mengobservasi efek anti kanker kemoterapi terhadap stress oksidatif yang dihasilkan oleh efek samping pengobatan kemoterapi dengan melihat konsentrasi SOD, GSH dan MDA dari sampel darah yang diambil dari vena di ekor tikus. Hasil yang diperoleh peneliti adalah tidak signifikan secara statistik, sehingga disimpulkan bahwa bahwa curcumin dan nanocurcumin tidak memodulasi SOD, GSH dan MDA pada terapi kanker ovariaum yang mendapat terapi cisplatin.

Ovarian cancer is the second most common gynaecological cancer, and is also one of cancer that has the highest incidence level in Indonesian women. One of the main chemotherapy that is used to treat ovarian cancer therapy is cisplatin. Cisplatin works by generating oxidative stress to fight the cancer, however this would act as double edged knife. While generating oxidative stress is good for the therapeutic purpose, it would cause harm to the body, such as causing organ toxicity and systemic toxicity. To reduce the side effect of cisplatin, curcumin can be used. Curcumin also has an anti-cancer effect that can enhance the therapeutic effect of cisplatin. However, curcumin has a low bioavailability. To overcome this, our research group developed a nanosized curcumin, called Nanocurcumin, with the size of 11,5 nm, it has been proven to increase the bioavailability of curcumin in the body. This research is in an in vivo experiment on female Wistar rats. This research will observe the effect of anticancer co-chemotherapy on oxidative stress, by looking into GSH, SOD and MDA concentration from blood samples that were collected from the vein of the rat's tail. Results obtained for GSH, SOD and MDA were statistically insignificant (p>0,05). In conclusion, curcumin and nanocurcumin don't modulate GSH, SOD and MDA in ovarian cancer model in rats treated with cisplatin.
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Depok: Fakultas Kedokteran Universitas Indonesia, 2021
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UI - Skripsi Membership  Universitas Indonesia Library
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