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"Latar Belakang: SARS-CoV-2 menyebabkan pandemi COVID-19 yang telah menyebar di seluruh dunia termasuk Indonesia. Pandemi membuat masyarakat umum menderita masalah psikologis, salah satunya adalah kecemasan. Kecemasan dapat terjadi sebagai akibat dari pembatasan sosial serta paparan media yang berlebihan. Kecemasan sendiri merupakan salah satu Tujuan: Menganalisis hubungan antara tingkat kecemasan terhadap SARS-CoV-2 dengan gangguan sendi temporomandibula di masa pandemi COVID-19 pada masyarakat Jabodetabek dan menganalisis hubungan antara faktor sosiodemografi (usia dan jenis kelamin) dengan tingkat kecemasan terhadap SARS-CoV-2 dan gangguan sendi temporomandibula di masa Metode: Desain penelitian ini adalah potong lintang pada 421 masyarakat Jabodetabek. Partisipan mengisi kuesioner Coronavirus Anxiety Scale bahasa Indonesia untuk mengukur kecemasan terhadap SARS-CoV-2 serta Indeks Diagnostik Temporomandibular Disorder untuk mengukur gangguan sendi temporomandibula. Pengambilan data dilakukan secara daring melalui google form pada bulan November 2021 hingga Desember 2021. Hasil Penelitian: Uji Chi-Square menunjukkan tingkat kecemasan terhadap SARS-CoV-2 tidak memiliki hubungan bermakna dengan gangguan sendi temporomandibula di masa pandemi COVID-19 pada masyarakat Jabodetabek (p=0.151). Uji Chi-Square juga menunjukkan hubungan yang tidak bermakna antara usia dengan tingkat kecemasan terhadap SARS-CoV-2 (p=1) serta jenis kelamin dengan tingkat kecemasan terhadap SARS-CoV-2 (p=0.719). Uji Chi-Square menunjukkan hubungan yang bermakna antara usia dengan gangguan sendi temporomandibula (p=0.008), namun tidak pada hubungan antara jenis kelamin dengan gangguan sendi temporomandibula (p=0.137).Kesimpulan: Tidak terdapat hubungan antara tingkat kecemasan terhadap SARS-CoV-2 dengan gangguan sendi temporomandibula di masa pandemi COVID-19 pada masyarakat Jabodetabek. Tidak terdapat hubungan antara faktor sosiodemografi (usia dan jenis kelamin) dengan kecemasan terhadap SARS-CoV-2 di masa pandemi COVID-19 pada masyarakat Jabodetabek. Terdapat hubungan antara usia dengan gangguan sendi temporomandibula, namun tidak antara jenis kelamin dengan gangguan sendi temporomandibula di masa pandemi COVID-19 pada masyarakat Jabodetabek.

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Background: SARS-CoV-2 causes the COVID-19 pandemic which has spread throughout the world, including Indonesia. The pandemic makes the general public suffer from psychological problems, one of which is anxiety. Anxiety can occur as a result of social impact as well as excessive media exposure. Anxiety is one of many risk factors for temporomandibular joint disorders.

Objective: This study aims to analyze the association between anxiety levels against SARS-CoV-2 and temporomandibular joint disorders during the COVID-19 pandemic in the Jabodetabek population and analyzing the association between sociodemographic factors (age and gender) and anxiety levels against SARS-CoV-2 as well as temporomandibular joint disorders in the COVID-19 pandemic in the Jabodetabek population.

Methods: Cross-sectional study was conducted to 421 Jabodetabek population. Participant filled out the Indonesian Coronavirus Anxiety Scale questionnaire to assess the anxiety levels against SARS-CoV-2 and the Indeks Diagnostik Temporomandibular Disorder to assess the temporomandibular joint disorder. Data were collected online via google form in November 2021 until December 2021.

Result: The Chi-Square test showed that the anxiety levels against SARS-CoV-2 did not have a significant association with temporomandibular joint disorders during the COVID-19 pandemic in the Jabodetabek population (p=0.151). The Chi-Square test also showed a non-significant association between age and anxiety levels against SARS-CoV-2 (p=1) as well as gender and anxiety levels against SARS-CoV-2 (p=0.719). The Chi-Square test showed a significant association between age and temporomandibular joint disorders (p=0.008), but not on the association between gender and temporomandibular joint disorders (p=0.137).

Conclusion: There was no association found between anxiety levels against SARS-CoV-2 and temporomandibular joint disorders during the COVID-19 pandemic in the Jabodetabek Population. There was no association found between sociodemographic factors (age and gender) and anxiety levels against SARS-CoV-2 during the COVID-19 pandemic in the Jabodetabek population. There was an association found between age and temporomandibular joint disorders, however no association was found between gender and temporomandibular joint disorders during the COVID-19 pandemic in the Jabodetabek population."

"Respons antibodi spesifik SARS-CoV-2 dapat diperoleh dari paparan virus ketika infeksi ataupun dari vaksinasi. Studi mengenai rasio CD4+/CD8+ sebagai penanda status imunitas masih belum banyak dilakukan pada dewasa sehat. Vitamin D yang memiliki efek imunomodulatori pada sistem imun adaptif dan alamiah, mampu memodulasi pembentukan antibodi dan regulasi dari sel T. Penelitian ini bertujuan melihat hubungan kadar 25(OH)D serum terhadap titer antibodi SARS-CoV-2 dan rasio CD4+/CD8+ sebagai penanda status imunitas individu. Studi potong lintang ini dilakukan terhadap tenaga kesehatan di tiga rumah sakit rujukan COVID-19 di Jakarta dan Depok pada periode Juli–Desember 2021. Pengambilan data yang dilakukan berupa wawancara kuesioner data sosiodemografik, pemeriksaan tanda-tanda vital, pengukuran antropometri, dietary assessment menggunakan 24-h food recall dan SQ-FFQ. Pengambilan sampel darah dilakukan untuk menilai kadar 25(OH)D serum, rasio CD4+/CD8+, dan titer antibodi SARS-CoV-2. Didapatkan 154 tenaga kesehatan usia 22-53 tahun dalam kondisi sehat dan tanpa riwayat penyakit kronis. Median asupan vitamin D subjek penelitian sebesar 2,42 mcg/hari (1,23–4,00) dengan 94,7% subjek memiliki asupan vitamin D yang kurang. Median kadar serum 25(OH)D pada subjek sebesar 14,4 ng/mL (9,50–18,62) dengan 81,8% subjek mengalami defisiensi dan 14,9% subjek mengalami insufisiensi vitamin D. Median rasio CD4+/CD8+ 1,14 (0,88–1,34), 85,7% subjek memiliki titer antibodi SARS-CoV-2 >250 U/mL dan 14,3% subjek memiliki titer antibodi ≤250. Tidak ditemukan adanya hubungan yang siginifikan antara kadar 25(OH)D dengan titer antibodi SARS-CoV-2 (p 0,209 OR 4,101 95% CI 0,45–37,04) dan Rasio CD4/CD8 (p 0,385 𝛃 -0,005 95% CI -0,0015–0,006). Asupan dan kadar vitamin D pada subjek penelitian masih tergolong rendah. Penelitian ini tidak berhasil membuktikan adanya hubungan antara kadar serum 25(OH)D dengan rasio CD4+/CD8+ dan titer antibodi SARS-CoV-2.

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SARS-CoV-2 specific antibody response can be generated from exposure to the virus during infection or from vaccination. There is limited data on CD4+/CD8+ ratio in healthy individuals as a marker of immunity status. Vitamin D, which has an immunomodulatory effect on both  innate and adaptive immune systems, is able to modulate antibody formation and regulation of T cells. This study aimed to examine the association between serum 25(OH)D levels and SARS-CoV-2 antibody titers along with CD4+/CD8+ ratio as a marker of immunity status. This cross-sectional study was conducted on healthcare workers at three COVID-19 referral hospitals in Jakarta and Depok in the period of July–December 2021. Data collection was carried out using questionnaire, examination of vital signs, anthropometric measurements, dietary assessment using 24-h food recall, and SQ-FFQ. Blood samples were taken to assess serum 25(OH)D levels, CD4+/CD8+ ratio, and SARS-CoV-2 antibody titers. 154 healthcare workers aged 22-53 years who were in good health and had no history of chronic disease were examined in this study. The median intake of vitamin D was 2.42 mcg/day (1.23-4.00), with 94.7% of participants having insufficient intake of vitamin D. The median serum 25(OH)D level was 14.4 ng/mL (9.50-18.62), with 81.8% participants are vitamin D deficiency and 14.9% are insufficient. Median CD4+/CD8+ ratio was 1.14 (0.88 to 1.34). 85.7% participants had SARS-CoV-2 antibody titers >250 U/mL, while 14.3% were below 250 U/mL. There was no significant relationship of serum 25(OH)D levels to SARS-CoV-2 antibody titers (p 0.209 OR 4.101 95% CI 0.45–37.04) and CD4+/CD8+ ratio (p 0.385 o-0.005 95% CI -0.0015–0.006). Vitamin D intake and serum 25(OH)D levels are relatively low. This study disproves relationship between serum 25(OH)D levels with CD4+/CD8+ ratio and SARS-CoV-2 antibody."

"Coronavirus disease atau COVID-19 merupakan pandemi global yang mudah menular melalui droplet dan populasi yang paling berisiko adalah lansia dengan tingkat kematian akibat COVID-19 di Indonesia per 27 Juni 2020 adalah sebanyak 42,9%. Hal tersebut dapat menimbulkan kecemasan tersendiri bagi lansia, terlebih lagi secara psikologis lansia lebih mudah cemas daripada populasi lain. Kecemasan diketahui merupakan salah satu faktor penting penyebab kerentanan terinfeksi Coronavirus. Maka dari itu penulisan ini dibuat untuk menemukan gambaran ansietas pada 10 lansia sebelum tindakan swab PCR SARS CoV-2 di RSUI. Data yang dipakai adalah data umum, data skrining COVID-19, riwayat penyakit dalam, riwayat merokok, dan kuesioner kecemasan menggunakan Geriatric Anxiety Inventory Short Form (GAI-SF). Berdasarkan data yang didapatkan, terdapat banyak faktor yang mungkin mempengaruhi munculnya ansietas pada lansia seperti kecemasan terhadap hasil swab PCR. Hasil pengkajian GAI-SF juga menyatakan bahwa semua lansia mengalami ansietas dengan dua diantaranya memiliki gejala GAD. Antar pasien juga memiliki beberapa kesamaan terkait COVID-19 dan hubungannya dengan kecemasan. Sebagai kesimpulan, masalah ansietas pada lansia terkait COVID-19 sebelum tindakan swab PCR memiliki nilai yang tinggi disebabkan karena beberapa faktor sehingga perlu diberikan rekomendasi khusus. Penulis merekomendasikan penyediaan kursi prioritas untuk lansia, bilik swab khusus lansia dengan aromaterapi dan musik relaksasi jika memungkinkan, dan edukasi terkait COVID-19 oleh perawat melalui selebaran maupun follow up secara daring.

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Coronavirus disease or called COVID-19 is a global pandemic that is easily transmitted through droplets and the population with higher risk of it are the elderly within death rate in Indonesia per June 27, 2020 is 42.9%. This problem can cause anxiety in the elderly even more as psychologically the elderly are more vulnerable to feel anxious than other populations. Whereas anxiety is one of the important factors causing decreased immunity which makes the elderly more susceptible to Coronavirus. Therefore, this paper was made to find an overview of anxiety in 10 elderly people before the swab polymerase chain reaction severe acute respiratory syndrome coronavirus-2 or swab PCR SARS CoV-2 procedure at RSUI. The data used are general data, COVID-19 screening data, history disease, smoking history, and anxiety questionnaires using Geriatric Anxiety Inventory Short Form (GAI-SF). Based on the data obtained, there are many factors that might influence the emergence of anxiety in the elderly such as anxiety about the swab PCR results. The results of the GAI-SF also stated that all respondents were experiencing anxiety with two of them were having general anxiety disorder symptoms. Furthermore, inter-patients also have some similarities related to COVID-19 and anxiety. In conclusion, the anxiety problem among elderly related to COVID-19 before the swab PCR procedure has a high value due to several factors so that special recommendations for the elderly should be given. The recommendation are using priority seats for the elderly, special swab PCR’s room with aromatheraphy and music of relaxation if possible, and nurses could give COVID-19 educations through flyer or follow up them within online educations"

"Latar Belakang: Prevalensi kecemasan pada mahasiswa lebih tinggi dibandingkan pada populasi umum. Hal ini dipengaruhi oleh beberapa faktor, antara lain faktor akademis, status sosioekonomi, faktor budaya, moral, psikologis, dan biologis. Tingginya tingkat kecemasan juga meningkatkan risiko terjadinya gangguan sendi temporomandibula pada mahasiswa.Tujuan: Mengetahui hubungan antara kecemasan dan gangguan sendi temporomandibula pada mahasiswa, serta mengetahui hubungan antara jenis kelamin, status sosioekonomi (tingkat pendidikan orang tua dan jumlah pendapatan keluarga), dan faktor budaya (asal daerah) dengan kecemasan dan gangguan sendi temporomandibula pada mahasiswa.Metode: Studi dengan desain cross-sectional berupa kuesioner online, yang disebarkan pada bulan November 2021 kepada mahasiswa Universitas Indonesia dengan jumlah responden 527 mahasiswa. Kuesioner yang diberikan merupakan State-Trait Anxiety Inventory yang terdiri dari 2 bagian dengan total 40 pertanyaan dan berfungsi untuk mengukur kecemasan, serta Temporomandibular Disorder Diagnostic Index (TMD-DI) yang berjumlah 8 pertanyaan dan digunakan untuk mengukur gangguan sendi temporomandibula.Hasil Penelitian: Uji chi-square menunjukkan adanya perbedaan bermakna (p<0.05) kecemasan, baik A-State maupun A-Trait, terhadap gangguan sendi temporomandibula. Uji korelasi kendall menunjukkan kecemasan, baik A-State maupun A-Trait, memiliki korelasi bermakna yang bersifat positif dan lemah terhadap gangguan sendi temporomandibula. Uji chi-square menunjukkan adanya perbedaan bermakna (p<0.05) jenis kelamin terhadap kecemasan,baik A-State maupun A-Trait. Kemudian, uji korelasi kendall menunjukkan jenis kelamin memiliki korelasi bermakna secara statistik terhadap kecemasan. Namun, uji continuity correctionmenunjukkan tidak adanya perbedaan bermakna (p 0.05) jenis kelamin terhadap gangguan sendi temporomandibula. Uji chi-square juga menunjukkan tidak terdapat perbedaan bermakna (p 0.05) status sosioekonomi (tingkat pendidikan orang tua dan jumlah pendapatan keluarga) dan faktor budaya (asal daerah) terhadap kecemasan dan gangguan sendi temporomandibula. Kesimpulan: Terdapat hubungan antara kecemasan, baik A-State maupun A-Trait, dengan gangguan sendi temporomandibula pada mahasiswa. Terdapat pula hubungan antara jenis kelamin dengan kecemasan, baik A-State maupun A-Trait, pada mahasiswa. Namun, tidak terdapat hubungan antara jenis kelamin dengan gangguan sendi temporomandibula, serta status sosioekonomi (tingkat pendidikan orang tua dan jumlah pendapatan keluarga) dan faktor budaya (asal daerah) dengan kecemasan, baik A-State maupun A-Trait, dan gangguan sendi temporomandibula pada mahasiswa.

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Background: The prevalence of anxiety in college students are higher than in general population. This can be influenced by several factors, including academic, socioeconomic status, cultural, moral, and also biological factors. The high level of anxiety also increases the risk of temporomandibular disorder in college students.

Objective: This study aims to find out the association between anxiety and temporomandibular disorder, and also find out the association between gender, socioeconomic status (parental education and monthly family income), and cultural factor (origin) with anxiety and temporomandibular disorder in college students.

Methods: A cross-sectional study using online questionnaire of 527 students from University of Indonesia, that conducted on November 2021. There are two given questionnaires, State-Trait Anxiety Inventory that consisted of two part and 40 questions in total to assess anxiety, and Temporomandibular Disorder Diagnostic Index that consisted of 8 questions tao assess temporomandibular disorder.

Result: The chi-square test showed that there was a statistically significant difference (p<0.05) of temporomandibular disorder based on anxiety, either A-State or A-Trait. The kendall correlation test showed that temporomandibular disorder, have positive and weak statistically significant correlation to anxiety, either A-State or A-Trait. The chi-square test showed that there was a statistically significant difference (p<0.05) of anxiety, either A-State or A-Trait, based on gender. The kendall correlation test also showed that gender, have positive and weak statistically significant correlation to anxiety, either A-State or A-Trait. However, the continuity correction test showed that there was no statistically significant difference (p 0.05) of temporomandibular disorder based on gender. The chi-square test also showed that there was no statistically significant difference (p 0.05) of anxiety, either A-State and A-Trait, and temporomandibular disorder based on socioeconomic status (parental education and monthly family income) and cultural factor (origin).

Conclusion: There was an association between anxiety, either A-State or A-Trait, and temporomandibular disorder, as well as gender and anxiety, either A-State or A-Trait, in college students. However, no association was found between gender and temporomandibular disorder, as well as socioeconomic status (parental education and monthly family income) and cultural factor (origin) with anxiety,either A-State or A-Trait, and temporomandibular disorder in college students. "

"Latar Belakang: Reseptor ACE2 tidak hanya terdapat pada paru-paru, tetapi juga pada saluran pencernaan yang memungkinkan terjadinya infeksi SARS-COV-2 pada enterosit, menimbulkan manifestasi klinis gastrointestinal, dan terdeteksinya RNA virus pada pemeriksaan swab anal. Studi lain di seluruh dunia menunjukkan hasil yang berbeda-beda serta belum didapatkan penelitian serupa di Indonesia. Tujuan: Penelitian ini bertujuan untuk mengetahui luaran klinis infeksi COVID- 19 pada pasien yang dilakukan swab anal, mendapatkan hubungan hasil pemeriksaan PCR SARS-CoV-2 swab anal dengan manifestasi klinis gastrointestinal dan derajat keparahan pada pasien COVID-19 di Indonesia. Metode: Merupakan cabang penelitian dari penelitian utama yang berjudul “Nilai RT-PCR Swab Anal untuk Diagnosis COVID-19 pada Orang Dewasa di Indonesia”. Penelitian ini merupakan studi analitik dengan desain potong lintang. Sampel penelitian merupakan pasien COVID-19 yang menjalani rawat inap di RSUPN dr. Cipto Mangunkusumo (RSCM), RS Mitra Keluarga Depok, RS Mitra Keluarga Kelapa Gading, dan RS Ciputra selama periode April 2020 sampai dengan Januari 2021. Dikumpulkan data demografi, manifestasi klinis, derajat keparahan, dan hasil swab anal PCR SARS-CoV-2.Hasil: 136 subjek penelitian dengan swab nasofaring positif dianalisis. 52 pasien (38,2%) dengan swab anal PCR SARS-CoV-2 positif dan 84 pasien (61,8%) dengan swab anal negatif. Manifestasi klinis saluran cerna tersering, yaitu: mual-muntah 69 pasien (50,7%), nafsu makan menurun sebanyak 62 pasien (45,6%), dan nyeri perut sebanyak 31 pasien (22,8). Terdapat 114 pasien (83,8%) tergolong dalam derajat ringan-sedang dan 22 pasien (16,2%) tergolong dalam berat-kritis. Terdapat hubungan yang bermakna secara proporsi statistik antara variabel hasil pemeriksaan PCR SARS-CoV-2 swab anal dengan manifestasi klinis gastrointestinal berupa keluhan diare atau mual-muntah (nilai p 0,031). Tidak terdapat hubungan yang bermakna secara proporsi statistik antara variabel hasil pemeriksaan PCR SARS-CoV-2 swab anal dengan derajat keparahan (nilai p 0,844).Simpulan: Terdapat hubungan antara hasil pemeriksaan PCR SARS-CoV-2 swab anal dengan manifestasi klinis gastrointestinal berupa keluhan diare atau mual- muntah dan tidak terdapat hubungan antara variabel hasil pemeriksaan PCR SARS- CoV-2 swab anal dengan derajat keparahan infeksi COVID-19.

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Background: ACE2 receptor is not only found in the lungs, but also in the digestive tract, which allows the occurrence of enterocyte infection, gastrointestinal clinical manifestations, and detection of viral RNA on anal swab PCR. Studies around the world show various results, yet there has been no similar study to be found in Indonesia.

Objective: This study aims to determine the clinical outcome of COVID-19 patients with gastrointestinal manifestations who were tested by anal swab, the relationship between anal swab PCR for SARS-CoV-2 test result with gastrointestinal clinical manifestations as well as the severity of COVID-19 patients in Indonesia.

Methods: This research is a branch of study titled. The Value of Anal Swab RT- PCR for COVID-19 Diagnosis in Adult Indonesian Patients. This is an analytical study with cross-sectional design. Samples were obtained from hospitalized COVID-19 patients at RSUPN dr. Cipto Mangunkusumo (RSCM), Mitra Keluarga Hospital Depok, Mitra Keluarga Kelapa Gading Hospital, and Ciputra Hospital from April 2020 to January 2021. Demographic data, clinical manifestations, severity, and SARS-CoV-2 PCR anal swab were collected.

Results: 136 subjects with positive nasopharyngeal swab were analyzed. Result showed that 52 patients (38.2%) had positive anal swabs PCR SARS-CoV-2 and 84 patients (61.8%) had negative anal swabs. Common gastrointestinal clinical manifestations were: nausea and vomiting in 69 patients (50.7%), anorexia in 62 patients (45.6%), and abdominal pain in 31 patients (22.8). There were 114 patients (83,8%) classified as mild-moderate and 22 patients (16,2%) as severe-critical. There was a statistically significant relationship between anal swab PCR for SARS- CoV-2 test result with gastrointestinal clinical manifestations (diarrhea or nausea- vomiting) (p value 0.031). There was no statistically significant relationship found between anal swab PCR for SARS-CoV-2 test result with the severity of COVID- 19 infection (p value 0.844).

Conclusions: There is a relationship between anal swab PCR SARS-CoV-2 test result with gastrointestinal clinical manifestations (diarrhea or nausea-vomiting) and there is no relationship between anal swab PCR SARS-CoV-2 test result with severity of COVID-19 infection."

"COVID-19 merupakan penyakit yang disebabkan oleh virus SARS-CoV-2 yang mengakibatkan pandemi global. Jakarta adalah salah satu kota di Indonesia dengan angka kasus dan kematian tertinggi akibat COVID-19. Salah satu cara paling efektif untuk mengurangi keparahan dan resiko penularan COVID-19 adalah dengan vaksinasi. Vaksin dapat merangsang respons imunitas humoral tubuh yang menghasilkan antibodi netralisasi. Selain vaksin, antibodi netralisasi dapat diinduksi secara natural oleh imunitas tubuh. Hybrid immunity merupakan gabungan antara antibodi netralisasi yang diinduksi secara natural dan yang diinduksi oleh vaksin. SARS-CoV-2 terus bermutasi memunculkan berbagai varian yang menyebabkan peningkatan jumlah kasus dan munculnya gelombang COVID-19 baru di Indonesia, yaitu gelombang Delta pada Juni 2021 dan gelombang Omicron pada Januari 2022. Penelitian ini bertujuan untuk mengevaluasi perubahan antibodi netralisasi 3 bulan setelah vaksinasi dosis lengkap dari beberapa jenis vaksin, yaitu vaksin virus inaktivasi (CoronaVac), vaksin viral vektor (ChAdOx1 nCoV-19), dan vaksin mRNA (BNT162b2) serta pengaruh riwayat infeksi SARS-CoV-2 pada penerima vaksin terhadap berbagai varian SARS-CoV-2 (Wuhan, Delta, Omicron B.1.1.529 dan BA.2). Penelitian dilakukan dengan menggunakan uji Surrogate Virus Neutralization Test (sVNT) yang memiliki prinsip kerja seperti enzyme-linked immunosorbent assay (ELISA) dan meniru interaksi antara receptor binding domain (RBD) dan angiotensin-converting enzyme 2 (ACE2) dalam pelat ELISA dengan RBD dan ACE2 yang telah mengalami pemurnian dengan sampel serum partisipan populasi umum (n = 76). Hasil penelitian menunjukkan adanya perbedaan signifikan antara antibodi netralisasi sebelum dan 3 bulan setelah vaksinasi dosis lengkap, tetapi tidak terdapat perbedaan signifikan pada antibodi netralisasi yang dihasilkan dari masing-masing jenis vaksin. Hal tersebut kemungkinan disebabkan oleh waktu pengambilan sampel setelah terjadi gelombang Omicron COVID-19 sehingga terjadi hybrid immunity yang menyebabkan tingginya kadar antibodi netralisasi yang merata pada setiap jenis vaksin. Partisipan dengan riwayat infeksi SARS-CoV-2 memiliki kadar antibodi netralisasi yang lebih tinggi. Terdapat perbedaan antibodi netralisasi yang signifikan terhadap berbagai varian SARS-CoV-2 dengan penurunan kadar antibodi netralisasi yang signifikan terhadap varian Omicron B.1.1.529 dan BA.2. Kesimpulan dari penelitian ini adalah vaksinasi dosis lengkap berhasil meningkatkan kadar antibodi netralisasi hingga 3 bulan pascavaksinasi yang dipengaruhi oleh riwayat infeksi SARS-CoV-2.

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COVID-19 is a disease caused by the SARS-CoV-2 virus which has resulted in a global pandemic. Jakarta is one of the cities in Indonesia with the highest number of COVID-19 cases and deaths. One of the most effective ways to reduce the severity and transmission risk of COVID-19 is by getting vaccinated. Vaccines can stimulate the body's humoral immune response to produce neutralizing antibodies. Apart from vaccines, neutralizing antibodies can be induced naturally by the body's immunity. Hybrid immunity is a combination of naturally induced neutralizing antibodies and those induced by vaccines. The continuously mutating SARS-CoV-2 has led to the emergence of various variants which have resulted in an increase in the number of cases and the emergence of new COVID-19 waves in Indonesia, namely the Delta variant which appeared in June 2021 and the Omicron variant in January 2022. This study aims to evaluate changes in neutralizing antibodies 3 months after complete doses of several types of vaccines, namely inactivated virus vaccine (CoronaVac), viral vector vaccine (ChAdOx1 nCoV-19), and mRNA vaccine (BNT162b2) and the effect of a history of SARS-CoV-2 infection in vaccine recipients against various variants SARS-CoV-2 (Wuhan, Delta, Omicron B.1.1.529 and BA.2). The study was conducted using the Surrogate Virus Neutralization Test (sVNT) test which has a working principle like the enzyme-linked immunosorbent assay (ELISA) and mimics the interaction between the receptor binding domain (RBD) and angiotensin-converting enzyme 2 (ACE2) in ELISA plates using RBD and ACE2 that had undergone purification with sera samples of general population participants (n = 76). The results showed that there were significant differences between the neutralizing antibodies before and 3 months after the full dose of vaccination, but there were no significant differences in the neutralizing antibodies produced from each type of vaccine. This was probably caused by the sampling time after the Omicron COVID-19 wave occurred, resulting in hybrid immunity which resulted in high levels of neutralizing antibodies that were evenly distributed in each type of vaccine. Participants with a history of SARS-CoV-2 infection had higher levels of neutralizing antibodies. There were significant differences in neutralizing antibodies against various variants of SARS-CoV-2 with a significant decrease in levels of neutralizing antibodies against Omicron B.1.1.529 and BA.2 variants. The conclusion of this study is that full dose vaccination has succeeded in increasing neutralizing antibody levels for up to 3 months after vaccination which are affected by a history of SARS-CoV-2 infection."

"Pada akhir tahun 2019 dilaporkan beberapa kasus pneumonia di Wuhan, Cina yang disebabkan oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Penyakit yang ditimbulkan oleh virus ini disebut sebagai coronavirus disease 2019 (COVID-19). Jumlah kasus COVID-19 terus mengalami peningkatan dan penyebarannya terjadi pada seluruh kelompok usia termasuk anak-anak. Pemeriksaan real-time reverse transcription-polymerase chain reaction (rRT-PCR) telah diotorisasi oleh Food and Drug Administration (FDA) dan pada pemeriksaan ini dikenal istilah cycle threshold (Ct). Nilai Ct sering dijadikan acuan dalam menentukan tingkat keparahan penyakit pada anak, akan tetapi masih terdapat kontroversi apakah nilai Ct berhubungan dengan tingkat keparahan penyakit. Penelitian ini bermaksud mencari hubungan bermakna antara nilai Ct khususnya gen ORF1ab dan gen N dengan tingkat keparahan COVID-19 pada anak yang dibagi menjadi tingkat keparahan ringan dan sedang sampai kritis. Didapat 52 responden anak dalam penelitian ini, dengan 24 responden terdeteksi gen ORF1ab dan 49 responden terdeteksi gen N. Rerata nilai Ct gen ORF1ab kelompok ringan (33,5 ± 4,4) lebih tinggi dibandingkan dengan kelompok sedang sampai kritis (31,0 ± 6,0). Median nilai Ct gen N kelompok ringan (34,8 [21,3 – 39,4]) lebih tinggi dibandingkan dengan kelompok sedang sampai kritis (31,7 [19,4 – 38,9]). Tidak didapatkan hubungan bermakna baik antara nilai Ct gen ORF1ab (nilai p = 0,25) maupun gen N (nilai p = 0,159) dengan tingkat keparahan COVID-19 pada anak. Berdasarkan hasil penelitian ini, diperlukan berbagai pertimbangan dalam menginterpretasi nilai Ct.

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At the end of 2019, several cases of pneumonia were reported in Wuhan, China caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The disease caused by this virus is known as Coronavirus Disease 2019 (COVID-19). The number of cases of COVID-19 continues to increase and its spread occurs in all age groups including children. Real-time reverse transcription-polymerase chain reaction (rRT-PCR) has been authorized by the Food and Drug Administration (FDA) and in this method a cycle threshold (Ct) value were obtained. The Ct value is often used as a reference in determining the clinical severity in children, but there is still controversy whether the Ct value is related to the clinical severity. This study intends to find a significant relationship between the Ct values, especially the ORF1ab gene and the N gene, with the COVID-19 clinical severity in children which is divided into mild and moderate to critical severity. There were 52 children in this study, with 24 children have ORF1ab gene detected and 49 children have N gene detected. The mean of ORF1ab gene Ct value in mild group (33.5 ± 4.4) was higher than moderate to critical group (31.0 ± 6.0). The median of N gene Ct value ​​in mild group (34.8 [21.3 – 39.4]) was higher than moderate to critical group (31.7 [19.4 – 38.9]). There was no significant relationship between the Ct value of the ORF1ab gene (p value = 0.25) and the N gene (p value = 0.159) with COVID-19 clinical severity in children. Based on the results of this study, various considerations are needed in interpreting the Ct value."

"Salah satu terapi COVID-19 adalah plasma konvalesen yang disiapkan Unit Transfusi Darah dari donor yang telah sembuh dari COVID-19. Plasma konvalesen mengandung antibodi netralisasi yang menghambat interaksi antara protein S dengan reseptor ACE2 dengan persyaratan minimal titer 1:160 sehingga diperlukan sistem deteksi antibodi netralisasi seperti tes serologi berbasis ELISA kompetitif yang mudah, murah, cepat dan tidak membutuhkan BSL 3 atau 2. Uji ini membutuhkan protein rekombinan spike S1 yang dapat diekspresikan pada sistem ekspresi mamalia. Penelitian ini bertujuan untuk mendeteksi antibodi spesifik SARS-CoV-2 pada plasma konvalesen COVID-19 menggunakan protein rekombinan Spike S1.Penelitian ini menggunakan plasmid pD609 sebagai vektor ekspresi yang terdapat gen spike S1. DNA ditransfeksi secara transien ke sel CHO. Immunostaining dilakukan setelah transfeksi untuk melihat ekspresi protein rekombinan spike S1 pada sel CHO. Supernatan media sel CHO post transfeksi dianalisis dengan western blot dan ELISA untuk melihat reaktifitas terhadap serum konvalesen COVID-19. Hasil immunostaining menunjukkan plasmid pD609 S1 Spike Foldon-His dapat mengekspresikan protein rekombinan spike S1 SARS-CoV-2 pada sel CHO. Hasil Western Blot dan ELISA menunjukkan supernatan media sel kultur CHO post transfeksi reaktif terhadap serum konvalesen COVID-19. Protein rekombinan spike S1 memiliki potensi untuk dikembangkan dan digunakan dalam uji antibodi spesifik namun hasil ekspresi protein masih rendah.

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One of the therapies for COVID-19 is convalescent plasma prepared by the Blood Transfusion Unit from donors who have recovered from COVID-19. Convalescent plasma contains neutralizing antibodies that inhibit the interaction between S protein and ACE2 receptors with a minimum requirement of a titer of 1:160 so that a neutalizing antibody detection system is needed such as a competitive ELISA-based serological test that is easy, inexpensive, fast, and does not require BSL 3 or 2. S1 spike recombinant protein that can be expressed in mammalian expression systems. This study aims to detect SARS-CoV-2 specific antibodies in COVID-19 convalescent plasma using recombinant Spike S1 protein. This study used the pD609 plasmid as an expression vector containing the spike S1 gene. DNA was transiently transfected into CHO cells. Immunostaining was performed after transfection to see the expression of the S1 spike recombinant protein in CHO cells. The post-transfected CHO cell media supernatans were analyzed by western blot and ELISA to see the reactivity to COVID19 convalescent serum. Immunostaining results showed that the plasmid pD609 S1 Spike Foldon-His could express the SARS-CoV-2 spike S1 recombinant protein in CHO cells. The results of Western blot and ELISA showed that the post-transfection CHO cell culture media supernatant was reactive to COVID-19 convalescent serum. S1 spike recombinant protein has the potential to be developed and used in specific antibody assays, but the results of protein expression is still low."

"Latar belakang: Pandemi coronavirus disease 2019 (COVID-19) secara global menyebabkan pemerintah Indonesia untuk menerapkan Pembatasan Sosial Berskala Besar (PSBB) sebagai upaya pencegahan transmisi COVID-19. Kebijakan ini berdampak pada perubahan gaya hidup anak melalui penutupan sekolah dan fasilitas rekreasional sehingga terjadi penurunan aktivitas fisik dan perubahan pola tidur anak. Penelitian ini bertujuan untuk mengetahui hubungan tingkat aktivitas fisik dengan gangguan tidur pada anak usia sekolah dasar di masa pandemi COVID-19.Metode: Penelitian ini menggunakan desain studi cross-sectional yang dilakukan pada 437 subjek. Penelitian ini menggunakan data sekunder tahun 2020 yang menggunakan Physical Activity Questionnaire for Older Children (PAQ-C) dan Children Sleep Habits Questionnaire-Abbreviated (CSHQ-A). Data yang diperoleh dianalisis menggunakan uji Chi-square.Hasil: Hasil penelitian menunjukkan sebanyak 60,87% subjek tidak aktif dan sebanyak 73,23% subjek mengalami gangguan tidur selama pandemi COVID-19. Hasil uji analisis statistik menunjukkan tidak terdapat hubungan bermakna antara tingkat aktivitas fisik dengan gangguan tidur (p=0,248).Kesimpulan: Sebagian besar anak usia sekolah dasar tidak aktif melakukan aktivitas fisik, kurang tidur pada hari biasa, tidur cukup pada akhir pekan, dan mengalami gangguan tidur selama pandemi COVID-19. Tidak terdapat hubungan bermakna antara tingkat aktivitas fisik dengan gangguan tidur pada anak usia sekolah dasar selama pandemi COVID-19.

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Introduction: Coronavirus disease 2019 (COVID-19) globally affected the Indonesian government to implement the large-scale social restriction to prevent the COVID-19 transmission. The policy has altered children’s lifestyles through the closure of schools and recreational facilities which caused the decline in children’s physical activity level and the alteration of children’s sleep pattern. This study aims to determine the relationship between elementary school-aged children’s physical activity level and sleep disorders during the COVID-19 pandemic.

Method: This study was conducted with a cross-sectional design on 437 subjects. The research used secondary data collected in 2020 to obtain the data through the Physical Activity Questionnaire for Older Children (PAQ-C) and the Children Sleep Habits Questionnaire-Abbreviated (CSHQ-A). The data were analyzed using the Chi-square analysis test.

Result: This study showed that 60,87% of the subjects were not physically active and 73,23% of the subjects had sleep disorders. The statistical analysis test showed no significant relationship (p 0.05) between children’s physical activity level and sleep disorders.

Conclusion: The majority of elementary school-aged children were not physically active and had sleep disorders during the COVID-19 pandemic. There was not a significant relationship between elementary school-aged children’s physical activity level and sleep disorders during the COVID-19 pandemic."

"Pandemi COVID-19 berdampak buruk terhadap kesehatan fisik dan mental. Hal ini disebabkan karena semakin menurunnya tingkat aktivitas fisik masyarakat selama pandemi berlangsung. Rendahnya tingkat aktivitas fisik juga berpengaruh terhadap kesehatan mental seseorang. Tidak terkecuali pada petugas layanan kesehatan yang memiliki risiko untuk terpapar COVID-19 lebih tinggi. Tujuan penelitian ini adalah mengetahui gambaran tingkat aktivitas fisik dan kesehatan mental (depresi, ansietas, stres) pada petugas layanan kesehatan di masa pandemi COVID-19. Metode: Penelitian potong lintang dengan menggunakan data primer. Pengambilan data di bulan Mei-Juni 2022 dan melalui metode kuesioner hybrid (daring dan luring). Aktivitas fisik dinilai dengan GPAQ (Global Physical Activity Questionaire) dan kesehatan mental dengan DASS-21 (Depression, Anxiety, Stress Scale-21). Hasil: Terdapat sebanyak 107 subjek yang ikut kedalam penelitian ini. Tingkat aktivitas fisik kurang didapatkan pada 55,1% petugas layanan kesehatan. Gejala kesehatan mental pada petugas layanan kesehatan didapatkan sebesar 23,4% untuk depresi, 31,8% untuk ansietas, dan 22,4% untuk stres. Dilakukan analisis bivariat untuk hubungan tingkat aktivitas fisik dengan depresi (PR = 0,881 (0,444-1,750); p>0,05), ansietas (PR = 0,915 (0,525-1,595); p>0,05), dan stres (PR = 0,961 (0,474-1,949); p>0,05). Kesimpulan: Tidak ditemukan hubungan yang bermakna antara tingkat aktivitas fisik dengan tingkat depresi, ansietas, dan stres. Kata Kunci: aktivitas fisik; ansietas; depresi; kesehatan mental; petugas layanan kesehatan; stres.

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The COVID-19 pandemic has devastating impact both on physical and mental health. This is due to decreasing level of physical activity among people during the pandemic. Low level of physical activity also affect a person's mental health. Healthcare workers are no exception, who might have higher risk of being exposed to COVID-19. The purpose of this study was to describe the level of physical activity and mental health (depression, anxiety, stress) among healthcare workers during the COVID-19 pandemic. Methods: Cross-sectional study using primary data. Data collection was conducted in May-June 2022 and using questionnaire delivered through hybrid method (online and offline). Physical activity was assessed by GPAQ (Global Physical Activity Questionnaire) and mental health by DASS-21 (Depression, Anxiety, Stress Scale-21). Results: There were 107 subjects who participated in this study. Inadequate levels of physical activity were found in 55.1% of health care workers. Mental health symptoms among healthcare workers was 23.4% for depression, 31.8% for anxiety, and 22.4% for stress. Bivariate analysis was conducted for the association between levels of physical activity and depression (PR = 0.881 (0.444-1.750); p>0.05), anxiety (PR = 0.915 (0.525-1.595); p>0.05), and stress (PR = 0.961 (0.474-1.949); p>0.05). Conclusion: There was no significant association between the level of physical activity and the level of depression, anxiety, and stress."