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"Telah dilakukan penelitian untuk mengidentifikasi efek krioprotektif madu lengkeng (Dimocarpus longan) terhadap integritas struktur folikel preantral dan profil ekspresi protein apoptosis jalur intrinsik. Sebanyak 24 ekor tikus (Rattus norvegicus L) dikelompokkan menjadi 8 kelompok, terdiri atas KKN, KKV (NaCl 0,9%), KKP1 (EG 7,5%), KKP2 (EG 15%), KP1 (EG 7,5% + ML 7,5%), KP2 (EG 7,5% + ML 15%), KP3 (EG 15% + ML 7,5%), dan KP4 (EG 15% + ML 15%). Pengamatan terhadap densitas,struktur folikel serta ekspresi protein Bax,Bcl2 dan Caspase3 dilakukan terhadap sayatan ovarium yang dibuat dengan metode parafin dengan pewarnaan Hematoksilin-Eosin (HE) dan imunohistokimia. Antibodi primer yang digunakan adalah antibodi poliklonal Rabbit Anti-Bax (A00183 Boster, USA), Rabbit Anti-Bcl-2 (A00040-2 Boster, USA) dan Active Caspase-3 Rabbit Polyclonal Antibody (ab4051 Abcam, UK) dan One Step Neopoly Detection System Kit (BGNK-0025 Biogear, USA). Identifikasi terhadap tiap tipe folikel preantral menggunakan mikroskop cahaya yang terhubung dengan perangkat lunak Image Raster dan IHC profiler. Hasil penelitian menunjukkan, efek krioprotektif madu lengkeng dapat meningkatkan densitas folikel, indeks folikel intak G2 dan G3, menurunkan indeks folikel G1, menekan ekspresi protein Bax dan caspase 3 serta meningkatkan ekspresi protein Bcl2. Dengan demikian, madu lengkeng memiliki potensi untuk dikembangkan sebagai krioprotektan ekstraselular alami dalam aplikasi vitrifikasi ovarium.

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A study was conducted to identify the cryoprotective effect of longan honey (Dimocarpus longan) on the structural integrity of the preantral follicle and the expression profile of the intrinsic pathway of apoptosis protein. A total of 24 rats (Rattus norvegicus L) were grouped into 8 groups, consisting of KKN, KKV (NaCl 0.9%), KKP1 (EG 7.5%), KKP2 (EG 15%), KP1 (EG 7.5% + LH 7.5%), KP2 (EG 7.5% + LH 15%), KP3 (EG 15% + LH 7.5%), and KP4 (EG 15% + LH 15%). Observations on the density, follicular structure and protein expression of Bax, Bcl2 and Caspase3 were carried out on ovarian sections made by paraffin method with Hematoxylin-Eosin (HE) staining and immunohistochemistry. The primary antibodies used were Rabbit Anti-Bax polyclonal antibody (A00183 Boster, USA), Rabbit Anti-Bcl-2 (A00040-2 Boster, USA) and Active Caspase-3 Rabbit Polyclonal Antibody (ab4051 Abcam, UK) and One Step Neopoly Detection System Kit (BGNK-0025 Biogear, USA). Identification of each type of preantral follicle using a light microscope connected to Image Raster software and an IHC profiler. The results showed that the cryoprotective effect of longan honey could increase follicle density, G2 and G3 intact follicle index, decrease G1 follicle index, suppress Bax protein expression and caspase 3 and increase Bcl2 protein expression. Thus, longan honey has the potential to be developed as a natural extracellular cryoprotectant in ovarian vitrification applications."

"Telah dilakukan penelitian untuk mengidentifikasi efek krioprotektif madu lengkeng (Dimocarpus longan) terhadap integritas struktur folikel preantral dan profil ekspresi protein apoptosis jalur intrinsik. Sebanyak 24 ekor tikus (Rattus norvegicus L) dikelompokkan menjadi 8 kelompok, terdiri atas KKN, KKV (NaCl 0,9%), KKP1 (EG 7,5%), KKP2 (EG 15%), KP1 (EG 7,5% + ML 7,5%), KP2 (EG 7,5% + ML 15%), KP3 (EG 15% + ML 7,5%), dan KP4 (EG 15% + ML 15%). Pengamatan terhadap densitas,struktur folikel serta ekspresi protein Bax,Bcl2 dan Caspase3 dilakukan terhadap sayatan ovarium yang dibuat dengan metode parafin dengan pewarnaan Hematoksilin-Eosin (HE) dan imunohistokimia. Antibodi primer yang digunakan adalah antibodi poliklonal Rabbit Anti-Bax (A00183 Boster, USA), Rabbit Anti-Bcl-2 (A00040-2 Boster, USA) dan Active Caspase-3 Rabbit Polyclonal Antibody (ab4051 Abcam, UK) dan One Step Neopoly Detection System Kit (BGNK-0025 Biogear, USA). Identifikasi terhadap tiap tipe folikel preantral menggunakan mikroskop cahaya yang terhubung dengan perangkat lunak Image Raster dan IHC profiler. Hasil penelitian menunjukkan, efek krioprotektif madu lengkeng dapat meningkatkan densitas folikel, indeks folikel intak G2 dan G3, menurunkan indeks folikel G1, menekan ekspresi protein Bax dan caspase 3 serta meningkatkan ekspresi protein Bcl2. Dengan demikian, madu lengkeng memiliki potensi untuk dikembangkan sebagai krioprotektan ekstraselular alami dalam aplikasi vitrifikasi ovarium.

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A study was conducted to identify the cryoprotective effect of longan honey (Dimocarpus longan) on the structural integrity of the preantral follicle and the expression profile of the intrinsic pathway of apoptosis protein. A total of 24 rats (Rattus norvegicus L) were grouped into 8 groups, consisting of KKN, KKV (NaCl 0.9%), KKP1 (EG 7.5%), KKP2 (EG 15%), KP1 (EG 7.5% + LH 7.5%), KP2 (EG 7.5% + LH 15%), KP3 (EG 15% + LH 7.5%), and KP4 (EG 15% + LH 15%). Observations on the density, follicular structure and protein expression of Bax, Bcl2 and Caspase3 were carried out on ovarian sections made by paraffin method with Hematoxylin-Eosin (HE) staining and immunohistochemistry. The primary antibodies used were Rabbit Anti-Bax polyclonal antibody (A00183 Boster, USA), Rabbit Anti-Bcl-2 (A00040-2 Boster, USA) and Active Caspase-3 Rabbit Polyclonal Antibody (ab4051 Abcam, UK) and One Step Neopoly Detection System Kit (BGNK-0025 Biogear, USA). Identification of each type of preantral follicle using a light microscope connected to Image Raster software and an IHC profiler. The results showed that the cryoprotective effect of longan honey could increase follicle density, G2 and G3 intact follicle index, decrease G1 follicle index, suppress Bax protein expression and caspase 3 and increase Bcl2 protein expression. Thus, longan honey has the potential to be developed as a natural extracellular cryoprotectant in ovarian vitrification applications."

"Indonesia merupakan negara tropis yang memiliki faktor resiko kulit terpapar sinar ultraviolet yang dapat menyebabkan peningkatan melanin. Melanin dihasilkan oleh melanosit, semakin tinggi jumlah melanin dapat mempengaruhi kecerahan warna kulit. Penilitian ini bertujuan memformulasi krim fitosom yang mengandung ekstrak biji buah lengkeng (Dimocarpus longan L.) menggunakan kasein sebagai emulgator serta melakukan uji penghambatan aktivitas tirosinase oleh krim yang dihasilkan. Metode yang digunakan adalah presipitasi untuk isolasi kasein, uji penghambatan aktivitas tirosinase, uji aktivitas antioksidan dengan menggunakan metode peredaman DPPH, pembatan fitosom dengan menggunakan metode penguapan pelarut dan hidrasi lapis tipis. IC50 ekstrak biji buah lengkeng dalam penghambatan aktivitas tirosinase sebesar 1876,4 ppm. Selain itu, karena ekstrak biji buah lengkeng mengandung beberapa polifenol sehingga dilakukan pengukuran aktifitas antioksidan dengan metode peredaman DPPH (2,2-Difenil-1-pikrihidrazil) dan diperoleh EC50 sebesar 6,5809 ppm. Untuk meningkatkan absorbsi polifenol yang bersifat polar ke dalam epidermis kulit, maka dibuat dalam bentuk fitosom. Fitosom yang dibuat dengan perbandingan ekstrak biji buah lengkeng : fosfatidilkolin Lipoid S75 (250 mg : 375 mg) dalam 50 ml Etanol 96%. Efisiensi penjerapan fitosom yang dihasilkan adalah sebesar 68,26 %. Fitosom yang diperoleh dimasukkan ke dalam formula sediaan krim dengan menggunakan kasein sebagai emulgator dan menghasilkan formula yang stabil baik pada stabilitas pada suhu 4±2°C, 27±2°C, 40±2°C dan pada cycling test. Kasein yang digunakan sebagai emulgator diisolasi dari susu cair bebas lemak dengan indeks aktivitas emulsi pada menit ke-0 dan ke-10 sebesar 0,0766 dan 0,290 m2g dan indeks kestabilan emulsi selama 3,487 menit

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Indonesia is a tropical country with a risk factor of skin exposed to ultraviolet that can cause increasing amount of melanin. Melanin is produced by melanocytes, the higher amount of melanin can affect the brightness of skin. This research aims is to formulate a cream containing fitosom extract from longan seed (Dimocarpus longan L.) using casein as emulsifier and tyrosinase inhibition assay from cream. The method used for isolation of casein is precipitation, tyrosinase inhibition assay, antioxidant activity was measured by the DPPH method, Phytosome are made using the solvent evaporation method and thin-layer hydration. The IC50 extract of longan seed have tyrosinase inhibition was 1876.4 ppm. That the extract of longan seed also have antioxidant activity was measured by the DPPH (2,2-diphenyl-1-pikrihidrazil) method and obtained EC50 is 6.5809 ppm. In order to improve the absorption of polyphenols into the epidermis, the extract was loaded into phytosome. Phytosome are made with extract of longan seed : phosphatidylcholine Lipoid S75 (250 mg: 375 mg) in 50 ml of ethanol 96%. Phytosome entrapment efficiency is 68.26 %. The phytosome loaded into cream preparation using casein as emulsifier and produce a formula that was stable both in stability at 4 ± 2 ° C, 27 ± 2 ° C, 40 ± 2 ° C and the cycling test. Casein is used as an emulsifier isolated from Skimmed milk with emulsion activity index at minute 0 and 10 by 0.0766 and 0.290 m2g and emulsion stability index for 3.487 minutes."

"Penelitian mengenai pengaruh kombinasi etilen glikol dan sari kurma terhadap struktur folikel preantral ovarium tikus (Rattus norvegicus l.) galur Sprague-Dawley pascavitrifikasi telah dilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi etilen glikol dan sari kurma dalam konsentrasi 3,75%; 7,5%; dan 15% terhadap morfologi dan persentase folikel preantral (folikel primordial, primer, dan sekunder) pada ovarium tikus setelah 48 jam vitrifikasi. Dua puluh satu ovarium tikus yang diisolasi pada fase proestrus terbagi menjadi 7 kelompok penelitian, yaitu KK, KKP 1, KKP 2, KKP 3, KP 1, KP 2, dan KP 3.Ovarium KK merupakan ovarium yang tidak divitrifikasi. Ovarium KKP 1, KKP 2, dan KKP 3 merupakan ovarium yang divitrifikasi dengan etilen glikol masing-masing berkonsentrasi 3,75%; 7,5%; dan 15%, sedangkan ovarium KP 1, KP 2, dan KP 3 merupakan ovarium yang divitrifikasi dengan kombinasi etilen glikol dan sari kurma pada tiga konsentrasi tersebut dengan perbandingan 1:1.Ovarium semua kelompok dibuat menjadi preparat dengan metode parafin dan pewarnaan HE, kemudian diamati di bawah mikroskop dengan perbesaran 400x dan hasil perhitungan folikel dianalisis secara statistik untuk mengetahui perbedaan antarkelompok.Berdasarkan hasil penelitian, rerata persentase folikel preantral dengan morfologi utuh tertinggi yaitu KK dan rerata persentase terendah yaitu KP 1. Hasil analisis folikel dengan morfologi utuh dengan uji Kruskal-Wallis dan analisis folikel dengan morfologi tidak utuh dengan ANOVA satu arah menunjukkan bahwa tidak berbeda nyata (P > 0,05). Kombinasi etilen glikol 7,5% dan sari kurma 7,5% lebih baik dalam menjaga keutuhan folikel preantral terutama folikel primordial pada ovarium tikus (Rattus norvegicus L.) galur Sprague-Dawley 48 jam pascavitrifikasi.

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The combination effects of ethylene glycol and palm dates juice on ovarian preantral follicles structure of Sprague-Dawley rats (Rattus norvegicus L.) post-vitrification was investigated. The aim of this research is to determine the combination effect of ethylene glycol and palm dates juice at concentration of 3.75%, 7.5%, and 15% respectively, to morphology and number of rats ovarian preantral follicles (primordial, primary, and secondary follicles) 48 hours post-vitrification. Twenty-one rat ovaries isolated on proestrous stage and categorized into 7 experimental groups: Normal Control group (NC), Treatment Control groups (TC 1, 2, 3), and Treatment groups (T1, 2, 3).

NC ovaries were not vitrified; TC 1, 2, 3 were vitrified with 3.75%, 7.5%, and 15% ethylene glycol solution, respectively; and T1, 2, 3 were vitrified with combination solution of ethylene glycol and palm dates juice at concentration 3.75%, 7.5%, and 15%, respectively with 1:1 ratio.

Ovaries from all experimental groups were made into histological slides with paraffin method and stained by HE and examined microscopically at x 400 magnification, then the results were statistically analyzed. Based on the results of the study, the mean percentage of preantral follicles with the highest intact morphology was NC and the lowest mean percentage was TC 1.

The results of statistical analysis with Kruskal-Wallis test for intact preantral follicles and one-way ANOVA for not intact preantral follicles showed that it is not significantly different (P > 0.05). The combination of 7.5% ethylene glycol and 7.5% palm dates juice is better at maintaining the integrity of preantral follicles especially the primordial follicles in the Sprague-Dawley rats ovary 48 hours post-vitrification than other Treatment groups (T1 and T3)."

"Preservasi sel atau jaringan erat kaitannya dengan cryoinjury akibat pembentukan kristal es yang akan merusak struktur memban sel dan peningkatan kadar radikal bebas yang dapat memicu kejadian apoptosis. Vitrifikasi ovarium menggunakan krioprotektan intraseluler saja, seperti etilen glikol, diketahui masih memiliki efek toksik terhadap sel. Madu berpotensi sebagai krioprotektan ekstraseluler dengan cara menjaga kestabilan membran sel selama proses vitrifikasi dan kombinasinya dengan etilen glikol dapat mengurangi efek toksik dari etilen glikol tersebut. Penelitian ini bertujuan untuk memanfaatkan madu kelengkeng dalam menjaga integritas folikel preantral ovarium dan menekan laju apoptosis folikel selama vitrifikasi, melalui analisis densitas, indeks folikel, dan rasio Bax/Bcl-2. Pulasan histologis digunakan untuk menganalisis integritas folikel menggunakan pewarnaan Hematoksilin-Eosin, serta pewarnaan Imunohistokimia untuk melihat ekspresi dari protein proapoptosis dan antiapoptosis. Pengamatan dilakukan menggunakan Optilab 3.0, software Image Raster, dan ImageJ. Hasil penelitian menunjukkan bahwa pemberian madu dengan konsentrasi 7,5% yang dikombinasikan dengan etilen glikol, baik 7,5% maupun 15%, dapat mempertahankan densitas dan indeks folikel preantral intak, serta menunjukkan rasio Bax/Bcl-2 yang lebih rendah dibandingkan kontrol vitrifikasi pada folikel preantral. Oleh karena itu, pemberian madu mampu mempertahankan integritas folikel preantral dan menekan laju apoptosis folikel preantral sesudah vitrifikasi.

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Preservation of cells or tissues is related to cryoinjury due to the formation of ice crystals that will damage the cell and increase free radicals that can trigger apoptosis. Ovarian vitrification using single intracellular cryoprotectants, such as ethylene glycol, is known to still have a toxic effect on cells. Honey has the potential as an extracellular cryoprotectant by maintaining the stability of cell membranes during the vitrification process and their combinations can reduce the toxic effects of ethylene glycol. This study aims to utilize longan honey to maintain the integrity of the ovarian preantral follicles and reduce the rate of follicle apoptosis during vitrification, through density, follicle index, and Bax/Bcl-2 ratio analysis. Histological staining was used to analyze the integrity of the follicles using Hematoxylin-Eosin staining, and Immunohistochemical staining to see the expression of proapoptotic and antiapoptotic proteins. Observations were made using Optilab 3.0, Image Raster, and ImageJ software. The results showed that honey with a concentration of 7.5% combined with ethylene glycol, both 7.5%, and 15%, could maintain follicle density and intact preantral follicle index, and showed a lower Bax/Bcl-2 ratio than the vitrified control in the preantral follicle. Therefore, honey was able to maintain the integrity of the preantral follicles and reduce the rate of apoptosis of the preantral follicles after vitrification."

"Tujuan Studi ini adalah untuk mengetahui pengaruh hipoksia terhadap pola ekspresi gen HIF-1α pada jantung tikus serta mengamati timbulnya apoptosis pada kardiomiosit akibat hipoksia sistemik.Metode Hewan coba (tikus Sprague-Dawley) dibagi secara acak menjadi 7 kelompok (n= 4 per kelompok): kelompok kontrol normoksia (oksigen atmosfir), dan beberapa kelompok hipoksia yang ditempatkan dalam sungkup-hipoksik (kadar O2 8%) selama 1, 3, 7, 14, 21, dan 28 hari. Pemeriksaan ekspresi gen HIF-1α dilakukan dengan real-time PCR dan apoptosis dengan metode TUNEL.Hasil Dibandingkan dengan kelompok normoksia, ekspresi gen HIF-1α meningkat secara bertahap sejalan dengan lamanya hipoksia dan mencapai puncak pada hari ke-21. Tidak ada sel yang terlabel dengan cara TUNEL pada kelompok kontrol. Dibandingkan dengan kontrol, indeks apoptotik meningkat sejalan dengan lamanya hipoksia. Tidak ada hubungan bermakna antara peningkatan ekspresi HIF-1α dengan peningkatan indeks apoptotik.Kesimpulan Hipoksia sistemik kronik mengakibatkan peningkatan ekspresi mRNA HIF-1α dan apoptosis pada kardiomiosit.

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AbstractAim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte.Methods Male Sprague-Dawley rats, were randomized into 7 groups (n= 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method.Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index.Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes."

"ABSTRAKManifestasi klinis yang menonjol pada demam berdarah dengue adalah kebocoran plasma karena gangguan pada sel endotel vaskular. Anti NS-1 dapat bereaksi silang dengan Protein Disulfide Isomerase (PDI) pada sel endotel. Jambu biji biasa digunakan untuk mengatasi gejala dengue namun belum pernah ada penelitian secara in vitro untuk mengetahui mekanisme senyawa aktif yaitu lycopene yang terdapat di dalamnya. Tujuan penelitian ini adalah untuk mengetahui apakah lycopene dapat menurunkan apoptosis yang ditandai dengan Annexin V dan Heme oxygenase -1 (HO-1). Penelitian ini menggunakan kultur Human Umbillical Vein Endothelial Cells (HUVEC) yang diberi stimulasi anti NS-1 dan terdiri atas 5 perlakuan yaitu kontrol positif, kontrol negatif, dan lycopene dosis 0.5, 1 dan 2 μM. Kontrol positif adalah HUVEC yang diberi anti NS-1 dan basitrasin karena basitrasin sudah diketahui bekerja sebagai anti PDI dan dapat menghambat apoptosis. Kontrol negatif adalah kultur HUVEC yang diberi anti NS-1 tanpa perlakuan lycopene. Hasil penelitian menunjukkan bahwa hanya Annexin V pada kontrol positif menunjukkan hasil yang rendah secara bermakna dibandingkan perlakuan lainnya. Hal ini menunjukkan bahwa lycopene dengan dosis 0.5, 1 dan 2 μM tidak dapat menghambat apoptosis. Kadar HO-1 pada semua perlakuan tidak menunjukkan perbedaan bermakna. Hal ini menunjukkan bahwa baik basitrasin maupun lycopene tidak mempengaruhi metabolisme HO-1. Dapat disimpulkan bahwa senyawa lycopene dengan dosis dosis 0.5, 1 dan 2 μM tidak menunjukkan pengaruh dalam apoptosis sel endotel setelah terjadi infeksi dengue.

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ABSTRACTProminent clinical manifestation of dengue hemorrhagic fever is plasma leakage due to malfunction of endothelial cells. There is cross reaction between anti NS-1 and Protein Disulfide Isomerase (PDI) on endothelial cells. Psidium guajava is commonly used to improve condition in dengue symptoms but there is no research yet that study the in vitro mechanism how lycopene, a compound in Psidium guajava, works in this case. So this study aimed to know whether lycopene will decrease apoptosis of endothelial cells marked by Annexin V and Heme oxygenase-1 (HO-1) This study used Human umbillical vein endothelial cells (HUVEC) that given anti NS-1 stimulation and consisted of positive control, negative control and lycopene treatment with 0.5, 1 and 2 μM dose. Positive control is HUVEC with anti NS-1 and bacitracin that known act as anti PDI and inhibit apoptosis. Negative control is HUVEC with anti NS-1. Results showed that Annexin V only in positive control had lower Annexin V significantly compared to other treatments. This showed that lycopene 0.5,1 and 2 μM was not able to inhibit apoptosis. HO-1 in all treatments did not show significant difference. This showed that either bacitracin nor lycopene did not give effect to HO-1 metabolism. It was concluded that lycopene with 0.5, 1 dan 2 μM dose has no effect in endothelial cell apoptosis after dengue infection."

"Penelitian dilakukan untuk mengevaluasi pengaruh penggunaan kombinasi etilen glikol (EG) sebagai krioprotektan intraseluler dan kuning telur sebagai krioprotektan ekstraseluler dengan variasi konsentrasi dalam mempertahankan jumlah dan morfologi folikel preantral ovarium tikus setelah vitrifikasi selama 48 jam. 20 Sprague-Dawley betina berumur 12 minggu digunakan sebagai hewan model penelitian. Folikel yang diuji merupakan folikel tahap preantral yang berasal dari ovarium kanan saja. Sampel ovarium utuh (n = 20) dibagi ke dalam 7 kelompok yaitu KK, KKP 1, KKP 2, KKP 3, KP 1, KP 2, dan KP 3.Kelompok kontrol (KK) merupakan ovarium segar tanpa vitrifikasi. Kelompok kontrol perlakuan (KKP 1, KKP 2, dan KKP 3) merupakan ovarium yang diberi perlakuan vitrifikasi dalam etilen glikol (EG) dengan konsentrasi berturut-turut 3,75%; 7,5%; dan 15%. Kelompok perlakuan (KP 1, KP 2, dan KP 3) merupakan ovarium yang diberi perlakuan vitrifikasi dalam kombinasi EG dan kuning telur (1 : 1) dengan konsentrasi masing-masing berturut-turut 3,75%; 7,5%; dan 15%. Setelah diisolasi dari tikus, ovarium KK segera difiksasi sedangkan ovarium KKP dan KP dibekukan dalam nitrogen cair (−196 ° C). Setelah 48 jam, sampel ovarium dicairkan dan difiksasi.Seluruh kelompok ovarium difiksasi selama 48 jam. Masing-masing ovarium lalu dibuat sayatan histologis dengan ketebalan 5 um, kemudian diwarnai dengan pewarna hematoksilin eosin (HE). Folikel preantral ovarium diamati keutuhan morfologinya dan dihitung jumlahnya. Data hasil pengamatan menunjukkan bahwa berdasarkan uji Kruskall-Wallis (P < 0,05) tidak terdapat perbedaan yang signifikan pada jumlah folikel preantral ovarium 48 jam pascavitrifikasi. Berdasarkan hal tersebut, dapat disimpulkan bahwa vitrifikasi menggunakan kombinasi etilen glikol (EG) dan kuning telur tidak berpengaruh dalam mempertahankan folikel preantral ovarium pascavitrifikasi.

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The study was conducted to evaluate the effect of combination of ethylene glycol (EG) as intracellular cryoprotectant and egg yolk as extracellular cryoprotectant with varied concentrations in maintaining morphology and counts of ovarian pre-antral follicles after vitrification for 48 hours. Twenty 12 week old Sprague-Dawley female rats were used as animal models. The follicles tested were preantral stage follicles from right ovary only. Whole ovary samples (n = 20) were divided into 7 groups, namely NC; TCG 1; TCG 2; TCG 3; TG 1; TG 2; and TG 3.

The control group (NC) consists of fresh ovaries without vitrification. The treatment control group (TCG 1; TCG 2; and TCG 3) consists of ovaries treated with vitrification in ethylene glycol (EG) with a concentration of 3.75%; 7.5%; and 15%, respectively. The treatment group (TG 1; TG 2; and TG 3) were ovaries treated with vitrification in a combination of EG and egg yolk (ratio 1: 1) with a concentration of 3.75%; 7.5%; and 15%, respectively. After being isolated from the rats, the NC ovaries were immediately fixated while the TCG and TG ovaries were frozen in liquid nitrogen (−196 °C). After 48 hours, the ovary sample is thawed and fixated.

All ovarian groups were fixated for 48 hours. Each of the ovaries was then made a histological incision with a thickness of 5 µm, then stained with a hematoxylin eosin (HE). Ovarian pre-antral follicles were observed for the integrity of the structure and counted. The data based on Kruskall-Wallis test (P < 0.05) show that there were no significant differences in the number of ovarian preantral follicles 48 hours after vitrification. It can be concluded that vitrification using a combination of ethylene glycol (EG) and egg yolk has no effect in maintaining ovarian preantral follicles after vitrification."

"[ABSTRAKLatar Belakang: Kanker prostat adalah kanker yang paling umum pada pria. Kanker terjadi karena hilangnya kontrol atas proliferasi sel dan apoptosis sehingga sel berproliferasi terus menerus tanpa ada kematian sel. Apoptosis diregulasi oleh beberapa protein tertentu diantaranya protein keluarga Bcl-2 dan protein kanal. Perkembangan kanker prostat memerlukan transformasi dari sel epitel yang normal menjadi sel ganas yang kehilangan kemampuan untuk mengakumulasi zinc. Salah satu efek utama zinc adalah mencegah pertumbuhan sel kanker prostat dengan menginduksi apoptosis dengan memfasilitasi proses pembentukan pori Bax yang memulai apoptogenesis mitokondria. Selain keluarga Bcl-2, VDAC1 juga berperan penting dalam proses apoptosis. Beberapa penelitian menyatakan Bcl-2 mempunyai kaitan erat dengan VDAC1 terkait proses apoptosis dan protein pro-apoptotik Bax juga secara langsung berinteraksi dengan VDAC yang kemudian menginduksi keluarnya sitokrom c dari membran mitokondria.Tujuan: Mengevaluasi ekspresi mRNA dari gen mengkode keluarga protein Bcl-2 (Bax dan Bcl-2) dalam proses apoptogenesis pada galur sel kanker prostat yg diinduksi oleh zinc; Mengevaluasi ekspresi mRNA dari gen VDAC1 dalam proses apoptogenesis pada galur sel kanker prostat yang diinduksi oleh zinc; Menganalisis hubungan antara ekspresi VDAC1 dengan protein keluarga Bcl-2 pada apoptogenesis galur sel kanker prostat.Desain: Penelitian ini menggunakan eksperimental in vitro dan analisis statistikMetode: Untuk memperbanyak galur sel kanker prostat (PC3) dilakukan kultur sel, kemudian diberi perlakuan dengan tiga kelompok (kontrol, zinc 20 μM dan staurosporin 0,16 μM). Selanjutnya dilakukan isolasi RNA dan elektroforesis RNA untuk mengetahui keutuhan RNA. Terakhir dilakukan qRT PCR yang kemudian datanya dianalisis secara statistika.Hasil: Ekspresi Bax, Bcl-2 dan VDAC1 pada galur sel kanker prostat (PC-3) yang diberi perlakuan zinc mengalami penurunan dibandingkan dengan kontrol (tidak diberi perlakuan). Akan tetapi penurunan ekspresi tersebut tidak bernilai signifikan karena nilai p > 0,05 (nilai signifikansi Bax = 0,309; nilai signifikansi Bcl-2 = 0,236; nilai signifikansi VDAC1 = 0,437). VDAC1 mempunyai korelasi yang signifikan (p < 0,05) dengan Bax (p = 0,01) dibandingkan dengan Bcl-2 (p = 0,118).Kesimpulan: Terjadi perubahan ekspresi pada setiap gen (Bax, Bcl-2 dan VDAC1) pada galur sel kanker prostat yang diberi perlakuan zinc dengan yang tidak diberi perlakuan, akan tetapi tidak bernilai signifikan. VDAC1 mempunyai korelasi yang bermakna dengan Bax dan mempunyai korelasi yang tidak bermakna dengan Bcl-2.

ABSTRACT

Background: Prostate cancer is the most common cancer in men. Cancer occurs due to loss control of cell proliferation and apoptosis thus continuously proliferating cells without cell death. Apoptosis is regulated by specific proteins including Bcl-2 family proteins and channel proteins. The development of prostate cancer requires the transformation of normal epithelial cells into malignant cells that lose the ability to accumulate zinc. One of the main effects of zinc is to prevent the growth of prostate cancer cells by inducing apoptosis by facilitating the process of pore formation Bax that started apoptogenesis mitochondrial. In addition to Bcl-2 family, VDAC1 also plays an important role in the process of apoptosis. Some studies suggest Bcl-2 has close links with related VDAC1 apoptosis and pro-apoptotic protein Bax also directly interact with VDAC which then induces the release of cytochrome c from the mitochondrial membrane.

Objective: To evaluate the expression of mRNA of the gene encoding the Bcl-2 family proteins (Bax and Bcl-2) in the process apoptogenesis on prostate cancer cell line that is induced by zinc; Evaluate the mRNA expression of genes in the process VDAC1 apoptogenesis on prostate cancer cell line induced by zinc; Analyzing the relationship between the expression of VDAC1 with Bcl-2 family proteins in prostate cancer cell lines apoptogenesis.

Design: This study used an experimental in vitro and statistical analysis

Methods: To reproduce the prostate cancer cell lines (PC3) performed cell culture, then treated with three groups (control, zinc 20 μM and staurosporin 0,16 μM). Furthermore, the isolation of RNA and RNA electrophoresis to determine the integrity of the RNA. Recently performed qRT PCR and the data were analyzed statistically.

Results: The expression of Bax, Bcl-2 and VDAC1 on prostate cancer cell line (PC-3) were treated with zinc decreased than the control (untreated). However, a decrease in the expression of no significant value because the value of p > 0.05 (Bax significant value = 0.309; the value of the significance of Bcl-2 = 0.236; VDAC1 significant value = 0.437). VDAC1 has a significant correlation (p < 0.05) with Bax (p = 0.01) than Bcl-2 (p = 0.118).

Conclusion: There is a change in the expression of each gene (Bax, Bcl-2 and VDAC1) in prostate cancer cell lines that treated with zinc than untreated, but no significant value. VDAC1 has a significant correlation with Bax and had no significant correlation with Bcl-2.;Background: Prostate cancer is the most common cancer in men. Cancer occurs due to loss control of cell proliferation and apoptosis thus continuously proliferating cells without cell death. Apoptosis is regulated by specific proteins including Bcl-2 family proteins and channel proteins. The development of prostate cancer requires the transformation of normal epithelial cells into malignant cells that lose the ability to accumulate zinc. One of the main effects of zinc is to prevent the growth of prostate cancer cells by inducing apoptosis by facilitating the process of pore formation Bax that started apoptogenesis mitochondrial. In addition to Bcl-2 family, VDAC1 also plays an important role in the process of apoptosis. Some studies suggest Bcl-2 has close links with related VDAC1 apoptosis and pro-apoptotic protein Bax also directly interact with VDAC which then induces the release of cytochrome c from the mitochondrial membrane.

Objective: To evaluate the expression of mRNA of the gene encoding the Bcl-2 family proteins (Bax and Bcl-2) in the process apoptogenesis on prostate cancer cell line that is induced by zinc; Evaluate the mRNA expression of genes in the process VDAC1 apoptogenesis on prostate cancer cell line induced by zinc; Analyzing the relationship between the expression of VDAC1 with Bcl-2 family proteins in prostate cancer cell lines apoptogenesis.

Design: This study used an experimental in vitro and statistical analysis

Methods: To reproduce the prostate cancer cell lines (PC3) performed cell culture, then treated with three groups (control, zinc 20 μM and staurosporin 0,16 μM). Furthermore, the isolation of RNA and RNA electrophoresis to determine the integrity of the RNA. Recently performed qRT PCR and the data were analyzed statistically.

Results: The expression of Bax, Bcl-2 and VDAC1 on prostate cancer cell line (PC-3) were treated with zinc decreased than the control (untreated). However, a decrease in the expression of no significant value because the value of p > 0.05 (Bax significant value = 0.309; the value of the significance of Bcl-2 = 0.236; VDAC1 significant value = 0.437). VDAC1 has a significant correlation (p < 0.05) with Bax (p = 0.01) than Bcl-2 (p = 0.118).

Conclusion: There is a change in the expression of each gene (Bax, Bcl-2 and VDAC1) in prostate cancer cell lines that treated with zinc than untreated, but no significant value. VDAC1 has a significant correlation with Bax and had no significant correlation with Bcl-2., Background: Prostate cancer is the most common cancer in men. Cancer occurs due to loss control of cell proliferation and apoptosis thus continuously proliferating cells without cell death. Apoptosis is regulated by specific proteins including Bcl-2 family proteins and channel proteins. The development of prostate cancer requires the transformation of normal epithelial cells into malignant cells that lose the ability to accumulate zinc. One of the main effects of zinc is to prevent the growth of prostate cancer cells by inducing apoptosis by facilitating the process of pore formation Bax that started apoptogenesis mitochondrial. In addition to Bcl-2 family, VDAC1 also plays an important role in the process of apoptosis. Some studies suggest Bcl-2 has close links with related VDAC1 apoptosis and pro-apoptotic protein Bax also directly interact with VDAC which then induces the release of cytochrome c from the mitochondrial membrane.

Objective: To evaluate the expression of mRNA of the gene encoding the Bcl-2 family proteins (Bax and Bcl-2) in the process apoptogenesis on prostate cancer cell line that is induced by zinc; Evaluate the mRNA expression of genes in the process VDAC1 apoptogenesis on prostate cancer cell line induced by zinc; Analyzing the relationship between the expression of VDAC1 with Bcl-2 family proteins in prostate cancer cell lines apoptogenesis.

Design: This study used an experimental in vitro and statistical analysis

Methods: To reproduce the prostate cancer cell lines (PC3) performed cell culture, then treated with three groups (control, zinc 20 μM and staurosporin 0,16 μM). Furthermore, the isolation of RNA and RNA electrophoresis to determine the integrity of the RNA. Recently performed qRT PCR and the data were analyzed statistically.

Results: The expression of Bax, Bcl-2 and VDAC1 on prostate cancer cell line (PC-3) were treated with zinc decreased than the control (untreated). However, a decrease in the expression of no significant value because the value of p > 0.05 (Bax significant value = 0.309; the value of the significance of Bcl-2 = 0.236; VDAC1 significant value = 0.437). VDAC1 has a significant correlation (p < 0.05) with Bax (p = 0.01) than Bcl-2 (p = 0.118).

Conclusion: There is a change in the expression of each gene (Bax, Bcl-2 and VDAC1) in prostate cancer cell lines that treated with zinc than untreated, but no significant value. VDAC1 has a significant correlation with Bax and had no significant correlation with Bcl-2.]"

"Kanker serviks termasuk salah satu jenis kanker dengan tingkat prevalensi kasus yang tinggi di Indonesia. Berdasarkan GLOBOCAN 2020, ditemukan 36.633 (36% dari total kasus kanker pada wanita) kasus kanker serviks baru dan 21.003 kematian. Pengobatan terhadap kanker serviks saat ini masih menimbulkan efek samping seperti resistensi obat pada sejumlah kasus sehingga diperlukan alternatif pengobatan. Salah satu senyawa yang memiliki potensi antikanker, yaitu Eucalyptol. Eucalyptol adalah senyawa utama dalam tumbuhan eukaliptus seperti Eucalyptus globulus. Studi pengaruh konsentrasi eucalyptol terhadap viabilitas dan deteksi apoptosis sel HeLa dilakukan dengan konsentrasi 25, 50, 100 dan 200 μg/mL. Uji viabilitas dengan WST-1 menunjukkan bahwa variasi konsentrasi eucalyptol tersebut belum dapat menekan viabilitas sel HeLa (p>0,05 dengan uji ANOVA). Selanjutnya, deteksi apoptosis (menggunakan pewarna Annexin V-FITC) dengan mikroskop fluoresens menunjukkan bahwa variasi konsentrasi eucalyptol dapat menginduksi apoptosis dibandingkan nekrosis. Meskipun demikian, belum dilakukan pengamatan pada sel hidup sehingga tidak diketahui persentase sel apoptosis dan nekrosis dalam populasi total. Pengujian lanjutan dengan pewarna DAPI yang mewarnai sel hidup dan metode kuantifikasi lainnya untuk validasi hasil pengamatan dengan mikroskop fluoresens diperlukan.

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Cervical cancer is one type of cancer with a high case prevalence rate in Indonesia. Based on GLOBOCAN 2020, found 36,633 (36% of total cancer cases in women) new cervical cancer cases and 21,003 deaths. Treatment of cervical cancer is currently still causing side effects such as drug resistance in a number of cases so that alternative treatment is needed. One of the compounds that have anticancer potential is Eucalyptol. Eucalyptol is the main compound in eucalyptus plants such as Eucalyptus globulus. The study of the effect of eucalyptol concentration on the viability and detection of apoptosis of HeLa cells was carried out at concentrations of 25, 50, 100 and 200 g/mL. The viability test with WST-1 showed that the variation in eucalyptol concentration had not been able to suppress HeLa cell viability (p>0.05 by ANOVA test). Furthermore, detection of apoptosis (using Annexin V-FITC dye) by fluorescent microscopy showed that variations in eucalyptol concentration could induce apoptosis rather than necrosis. However, no observations have been made on living cells so that the percentage of apoptotic and necrotic cells in the total population is unknown. Further testing with DAPI dye that stains living cells and other quantification methods for validation of observations by fluorescent microscopy is required.

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