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"Stomatitis Aphtous Rekuren (SAR) merupakan kondisi inflamasi kronis yang terjadi pada mukosa mulut yang ditandai dengan terbentuknya ulser. Patch mukoadhesif dapat ditujukan untuk melindungi ulser dari lingkungan mulut dan meningkatkan efektivitas terapi RAS dengan memperpanjang durasi perlekatan. Kurkumin merupakan senyawa yang memiliki aktivitas antiinflamasi sedangkan brazilin memiliki aktivitas antimikroba yang keduanya dapat digunakan untuk terapi RAS. Oleh sebab itu, tujuan penelitian ini adalah membuat dan mengevaluasi patch mukoadhesif dari ekstrak kayu secang dan kurkumin untuk terapi RAS. Patch mukoadhesif dibuat dengan teknik solvent casting dengan menggunakan polikarbofil dan etil selulosa sebagai polimer. Bahan padat dilarutkan dengan etanol lalu ditambahkan plasticizer kemudian dituang ke dalam cawan petri. Patch dibuat dengan 3 formulasi kurkumin-ekstrak kayu secang yang berbeda, yaitu 1:1 (F1), 3:1 (F2), dan 1:3 (F3). Patch yang terbentuk dievaluasi sifat fisiko-mekanik serta profil pelepasannya. Patch memiliki bobot yang seragam dengan ketebalan kurang dari 0,1 mm. Nilai kekuatan tarik sekitar 10,0 N/cm2, kekuatan mukoadhesif berkisar 6,7 sampai 7,5 N/cm2 dan mampu melekat hingga 2 jam. Dalam waktu 2 jam, brazilin telah terdisolusi sebanyak 100,58 % (F1) hingga 104,75% (F3) sedangkan kurkumin terdisolusi sebanyak 97,15% (F3) hingga 101,64% (F1). Secara keseluruhan, patch dapat dibuat dan memiliki karakteristik fisiko-mekanik dan profil pelepasan obat yang baik.

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Recurrent Aphtous Stomatitis (RAS) is a chronic inflammatory disease of the oral mucosa characterized by the formation of ulcers. Mucoadhesive patch is designed to protect the ulcers from the oral environment and increase the effectiveness of RAS therapy by prolonging the duration of attachment. Curcumin is compound that have antiinflammatory activity while brazilin have antimicrobial activity, both of which can be used for RAS therapy. For those reasons, this study aimed to produce and evaluate mukoadhesive patch containing sappanwood extract and curcumin for RAS therapy. Mucoadhesive patch were prepared by solving casting method using polycarbophil and ethyl cellulose as polymers. The patch were made in 3 formulations with different concentration of curcumin and sappanwodd extract, namely 1:1 (F1), 3:1 (F2), and 1:3 (F3). Mucoadhesive patch were evaluated of physicomechanical properties and dissolution profile. The patch of all formulations have a uniform weight with a thickness of less than 0,1 mm. The tensile strength of the patch were around 10,0 N/cm2 and mucoadhesive strength were in range of 6,7 to 7,5 N/cm2 and was able to adhere for up to 2 hours. Within 2 hours, the release of brazilin reached 100,58 % (F1) to 104,75% (F3) while the release of curcumin was 97,15% (F3) to 101,64% (F1). In conclusion, the patch has been produced with good physicomechanical properties and drug release profile."

"Latar Belakang: Mukositis merupakan salah satu efek samping yang timbul akibat kemoterapi. Mukositis menyebabkan timbulnya rasa sakit, ketidaknyamanan, kesulitan berbicara, menelan, makan, minum, kekurangan nutrisi, kelemahan sistemik hingga infeksi. Probiotik mengandung mikroorganisme nonpatogen yang memberikan manfaat bagi kesehatan dan membantu dalam pencegahan inflamasi pada rongga mulut. Beberapa studi telah melaporkan manfaat probiotik bagi kesehatan oral. Tujuan: Menganalisis efek probiotik Lactobacillus casei terhadap keadaan klinis mukosa oral pada anak dengan Leukemia Limfositik Akut yang menjalani kemoterapi sebelum berkumur probiotik L.casei, setelah berkumur L.casei selama 7 hari dan 14 hari. Metode Penelitian: Penelitian dilakukan di RS Kanker Dharmais dan RS Kramat 128. Sebelas pasien yang memenuhi kriteria. Tiga hari setelah pemberian kemoterapi, peneliti memeriksa keadaan klinis rongga mulut menggunakan Oral Assessment Guide (OAG). Pasien kemudian mulai berkumur probiotik L.casei dua kali sehari selama 7 hari dan 14 hari. Skor OAG diperiksa kembali setelah berkumur probiotik L.casei selama 7 hari dan 14 hari. Hasil: Terdapat penurunan skor OAG yang bermakna antara sebelum berkumur dengan setelah berkumur probiotik L.casei selama 7 hari dan 14 hari. Kesimpulan: Probiotik L.casei memberikan efek pada anak dengan leukemia limfositik akut yang menjalani kemoterapi dan dapat menjadi terapi alternatif terhadap mukositis oral.

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Mucositis is one of the side effects induced by chemotherapy. It results in pain, discomfort, difficulties in talking, swallowing, eating, drinking, poor nutrition, systemic weakness and life-threatening infections. Probiotics contain nonpathogenic live microorganisms that give benefit to our health and help in preventing inflammation in the oral cavity. Several studies have reported the use of probiotics for oral health purposes. Objective: To analyze the effect of probiotic Lactobacillus casei to clinical appearance of oral mucosa in children with Acute Lymphocytic Leukemia that underwent the chemotherapy process before gargling, 7 days after gargling, and 14 days after gargling with probiotic. Methods: The study was held in National Cancer Hospital “Dharmais” and Kramat 128 Hospital. Eleven patients were meet the criteria. Three days after the chemotherapy started, the researcher checked the patient’s oral mucosa condition using Oral Assessment Guide (OAG). Then the patient started to gargle the probiotic twice a day for 7 days and the researcher rechecked the score of oral mucositis after 7 days and 14 days. Results: There was a significance decreasing OAG score between before gargling with 7 days and 14 days after gargling L.casei probiotics. Conclusion: L.casei probiotics gave effects in children with acute lymphocytic leukemia during chemotherapy and could be an alternative therapy for oral mucositis."

"Stomatitis Aftosa Rekuren (SAR) merupakan penyakit mulut ditandai dengan ulkus berwarna putih-kekuningan yang umumnya sembuh selama 14 hari. Berbagai bentuk sediaan memiliki waktu kontak terbatas dengan lesi sehingga dapat mengurangi efek terapinya. Aktivitas anti-inflamasi, antiulkus, antioksidan, antibakteri glycyrrhizin (GL) dan ekstrak kayu secang sudah banyak dilaporkan tetapi belum ada yang menguji efeknya pada penyembuhan ulkus oral. Penelitian dilakukan bertujuan untuk membuat film mukoadhesif menggunakan zat aktif GL dan ekstrak kayu secang dengan karakteristik yang baik yang dapat diterima, memiliki aktivitas anti-inflamasi, dan tidak mengiritasi. Uji aktivitas anti-inflamasi zat aktif dilakukan sebagai uji pendahuluan penentuan dosis formulasi. Sembilan formula dengan variasi chitosan (CH) dan propilen glikol (PG) diuji terhadap parameter indeks mengembang, kekuatan mukoadhesif, waktu mukoadhesif, ketahanan regangan, dan pH permukaan. Formula optimal film dievaluasi karakteristik fisik, aktivitas anti-inflamasi, dan iritasinya. Total 3% kombinasi GL:ekstrak kayu secang (2:1) merupakan dosis optimal untuk formulasi. Formula optimal film (CH 0,53%; PG 3,00%) memiliki karakteristik sediaan film mukoadhesif yang baik yaitu mengembang >200%; melekat kuat pada mukosa selama 180,67 ± 9,85 menit; pH 6,39 ± 0,02 sama dengan rongga mulut; tahan terhadap lipatan >300 kali; stabil; dan tidak mengiritasi. Formula optimal film secara signifikan (p<0,05) menurukan diameter ulkus >90% sejak hari ke-4 dan jumlah leukosit mendekati normal yaitu 8975 ± 435,5/μL dibandingkan triamcinolone salep yaitu 9575 ± 415,1/μL. Pengamatan histologi menunjukkan formula optimal film memberikan profil regenerasi jaringan mirip dengan mukosa oral yang sehat. Formula optimal film dengan 3% kombinasi GL:ekstrak kayu secang (2:1) yang dihasilkan disimpulkan berpotensi dikembangkan sebagai alternatif pengobatan untuk SAR

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Recurrent Aphthous Stomatitis (RAS) is the oral mucosal lesions characterized by round ulcers with yellow-white color and heals up to 14 days. Many commercialized forms of treatment have limited residence time with the lesion that may decrease therapeutic efficiency. The anti-inflammatory, anti-ulcer, antioxidant, antibacterial properties of glycyrrhizin (GL) and C. sappan extract (CSE) have been demonstrated in many recent studies but no study has demonstrated the effect on the oral mucosal ulcer. The objective of this study was to optimize mucoadhesive oral film containing GL and CSE that is aesthetically acceptable, provides anti-inflammatory activity, and not irritant. Anti-inflammatory activity of GL and CSE was conducted as the preliminary study to determine the dosage of the formulation. All nine experimental runs with the various chitosan (CH) and propylene glycol (PG) concentrations were optimized against swelling index, mucoadhesive strength, residence time, tear resistance, and surface pH then physical characteristics, anti-inflammatory activity, and irritancy of the optimum formula were evaluated. Combination of 3% GL:CSE (2:1) showed the optimum dosage for formulation. The optimum formula (0.53% CH; 3.00% PG) showed a swelling index >200%; residence time up to 180.67 ± 9.85 minutes; pH 6.39 ± 0.02 similar to oral cavity; folding endurance >300 times; physical stable; and not irritant. The optimum formula was significantly (p<0.05) decreased the ulcer size up to >90% since day 4 with the leukocyte number 8975 ± 435.5/μL that was similar to the normal value compared to the triamcinolone paste 9575 ± 415.1/μL. In addition, the histological examination from optimum formula treatment showed a similar tissue regeneration profile with the healthy oral mucosa. This study was concluded that the mucoadhesive film containing combination of 3% GL:CSE (2:1) may be potential as the alternative treatment for RAS."

"Stomatitis Aftosa Rekuren (SAR) merupakan penyakit mulut ditandai dengan ulkus berwarna putih-kekuningan yang umumnya sembuh selama 14 hari. Berbagai bentuk sediaan memiliki waktu kontak terbatas dengan lesi sehingga dapat mengurangi efek terapinya. Aktivitas anti-inflamasi, antiulkus, antioksidan, antibakteri glycyrrhizin (GL) dan ekstrak kayu secang sudah banyak dilaporkan tetapi belum ada yang menguji efeknya pada penyembuhan ulkus oral. Penelitian dilakukan bertujuan untuk membuat film mukoadhesif menggunakan zat aktif GL dan ekstrak kayu secang dengan karakteristik yang baik yang dapat diterima, memiliki aktivitas anti-inflamasi, dan tidak mengiritasi. Uji aktivitas anti-inflamasi zat aktif dilakukan sebagai uji pendahuluan penentuan dosis formulasi. Sembilan formula dengan variasi chitosan (CH) dan propilen glikol (PG) diuji terhadap parameter indeks mengembang, kekuatan mukoadhesif, waktu mukoadhesif, ketahanan regangan, dan pH permukaan. Formula optimal film dievaluasi karakteristik fisik, aktivitas anti-inflamasi, dan iritasinya. Total 3% kombinasi GL:ekstrak kayu secang (2:1) merupakan dosis optimal untuk formulasi. Formula optimal film (CH 0,53%; PG 3,00%) memiliki karakteristik sediaan film mukoadhesif yang baik yaitu mengembang >200%; melekat kuat pada mukosa selama 180,67 ± 9,85 menit; pH 6,39 ± 0,02 sama dengan rongga mulut; tahan terhadap lipatan >300 kali; stabil; dan tidak mengiritasi. Formula optimal film secara signifikan (p<0,05) menurukan diameter ulkus >90% sejak hari ke-4 dan jumlah leukosit mendekati normal yaitu 8975 ± 435,5/μL dibandingkan triamcinolone salep yaitu 9575 ± 415,1/μL. Pengamatan histologi menunjukkan formula optimal film memberikan profil regenerasi jaringan mirip dengan mukosa oral yang sehat. Formula optimal film dengan 3% kombinasi GL:ekstrak kayu secang (2:1) yang dihasilkan disimpulkan berpotensi dikembangkan sebagai alternatif pengobatan untuk SAR.

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Recurrent Aphthous Stomatitis (RAS) is the oral mucosal lesions characterized by round ulcers with yellow-white color and heals up to 14 days. Many commercialized forms of treatment have limited residence time with the lesion that may decrease therapeutic efficiency. The anti-inflammatory, anti-ulcer, antioxidant, antibacterial properties of glycyrrhizin (GL) and C. sappan extract (CSE) have been demonstrated in many recent studies but no study has demonstrated the effect on the oral mucosal ulcer. The objective of this study was to optimize mucoadhesive oral film containing GL and CSE that is aesthetically acceptable, provides anti-inflammatory activity, and not irritant. Anti-inflammatory activity of GL and CSE was conducted as the preliminary study to determine the dosage of the formulation. All nine experimental runs with the various chitosan (CH) and propylene glycol (PG) concentrations were optimized against swelling index, mucoadhesive strength, residence time, tear resistance, and surface pH then physical characteristics, anti-inflammatory activity, and irritancy of the optimum formula were evaluated. Combination of 3% GL:CSE (2:1) showed the optimum dosage for formulation. The optimum formula (0.53% CH; 3.00% PG) showed a swelling index >200%; residence time up to 180.67 ± 9.85 minutes; pH 6.39 ± 0.02 similar to oral cavity; folding endurance >300 times; physical stable; and not irritant. The optimum formula was significantly (p<0.05) decreased the ulcer size up to >90% since day 4 with the leukocyte number 8975 ± 435.5/μL that was similar to the normal value compared to the triamcinolone paste 9575 ± 415.1/μL. In addition, the histological examination from optimum formula treatment showed a similar tissue regeneration profile with the healthy oral mucosa. This study was concluded that the mucoadhesive film containing combination of 3% GL:CSE (2:1) may be potential as the alternative treatment for RAS."

"Stomatitis aphthous rekuren (SAR) adalah lesi yang umum terjadi pada mukosa mulut yang ditandai dengan gejala inflamasi dan ulkus berwarna putih kekuningan dengan bentuk bulat atau oval. Banyak obat antiseptik, anestesi lokal, dan kortikosteroid telah digunakan sebagai terapi SAR. Namun, penggunaan yang berulang dapat menyebabkan efek samping dan resistensi obat. Berdasarkan penelitian sebelumnya, kurkumin dan brazilin memiliki efek sebagai antiinflamasi dan antioksidan, tetapi belum ada penelitian terkait kombinasi keduanya sebagai terapi SAR. Film hidrogel dirancang sebagai pembalut untuk memisahkan lesi mukosa dari lingkungan mulut, sehingga dapat meningkatkan efektivitas terapi. Tujuan dari penelitian ini adalah untuk mengembangkan film hidrogel berbasis alginate-kitosan dengan zat aktif kurkumin dan ekstrak kayu secang untuk SAR. Film hidrogel dibuat menjadi 3 formulasi, dengan perbedaan konsentrasi CaCl2 0,3%(F1); 0,4%(F2); dan 0,5%(F3). Film yang dihasilkan dievaluasi pH permukaan, ketahanan pelipatan, kekuatan tarik, indeks mengembang, profil pelepasan obat, kekuatan dan durasi mukoadhesif. Film memiliki ketebalan 0,01 mm, dengan pH permukaan berada pada rentang 6,9. Indeks mengembang film F1 merupakan yang tertinggi. Kekuatan mukoadhesif film berada pada rentang 4,72 N/cm2 (F3) hingga 4,88 N/cm2 (F1) serta memiliki waktu mukoadhesif tertinggi 11 menit (F1). Pelepasan kurkumin dari film antara 67-70% dan brazilin mencapai 100% selama 2 jam. Film F1 dengan konsentrasi CaCl2 0,3% menunjukkan karakteristik fisik yang paling baik

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Recurrent aphthous stomatitis (RAS) is a common lesion of the oral mucosa characterized by inflammatory symptoms and yellowish-white ulcers with a round or oval shape. Many antiseptic drugs, local anaesthetics, and corticosteroids have been used as RAS therapies. However, repeated use can cause side effects and drug resistance. In previous studies, curcumin and brazilin exhibit anti-inflammatory and antioxidant action, but there have been no study regarding the combination of them as RAS therapies. The hydrogel film is designed as a dressing to separate the mucosal lesions from the oral environment, thereby increasing the effectiveness of therapy. The purpose of this study is to develop an alginate-chitosan-based hydrogel film with the active substances curcumin and sampan wood extract for RAS. The hydrogel film was made into 3 formulations, with different concentrations of CaCl2 0.3%(F1); 0.4%(F2); and 0.5%(F3). The resulting film was evaluated for folding resistance, tensile strength, swelling index, drug release profile, mucoadhesive strength and duration. The film had a thickness of 0.01 mm, with a surface pH in the range of 6.9. The F1 film swelling index was the highest. The mucoadhesive strength of the film was in the range of 4.72 N/cm2 (F3) to 4.88 N/cm2 (F1) and had the highest mucoadhesive time of 11 minutes (F1). The release of curcumin from the film was between 67-70% and brazilin reached 100% for 2 hours. F1 film with 0,3% CaCl2 concentration exhibited the best physical characteristics"

"Oral lichen planus (OLP) is a rather common oral disease, mainly affecting adults, occurring more often in women than in men. The etiopathogenesis is still unclear. The diagnosis may be cumbersome, even in the presence of a biopsy. In addition, there are several lesions that may resemble lichen planus (lichenoid lesions) both clinicallyand histopathologically. Treatment of OLP can only be symptomatic and usually consists of topical application of corticosteroids. The disease is characterized by remissions and exacerbations and may persist in some patients lifelong. There is an ongoing debate in the literature as whether OLP is a potentially malignant disease. Because of this uncertainty, annual follow-up is advised."

"Indonesian Journal of Dentistry 2006; Edisi Khusus KPPIKG XIV: 215-220The purpose of this study was to disclose one of the etiopathogenesis of recurrent aphthous stomatitis (RAS) at molecular level by analyzing the expression of protein anomaly in oral mucosa. This was a cross-sectional explorative and analytic observational study. Samples, who met inclusion and exclusion criteria, were taken from total population. Samples of protein swab were obtained from oral mucosa, serum weretaken from 15 patients with major RAS, 20 patients with minor RAS and 15 were control. The characterization of protein anomaly expressed on the surface of oral mucosa epithelium was carried out using SDS-PAGE 12% and Westemblot methods. The result of oral mucosa protein anomaly expression analysis in patients with major RAS using SDS-PAGE 12% revealed five protein bands with molecular weights of 87,65, 30, 25, and 20 kDa. In minor RAS cases with protein anomaly expression there were four proteins with molecular weights of 87, 65, 25, and 20 kDa, and the protein in remission RAS had four proteins bands with molecular weight of 87, 65, 25 and 20 kDa. The band disappearances by using Westemblot test, of 30 kDa of major cases, 87 and 20 kDa of minor cases and 20 and 25 kDa of remission cases, indicated that those patients were not reacted with polyclonal antibodies of rabbit serum; therefore they had no role in the induction of RAS. ln conclusion, the antigenic protein expressed in oral mucosa of major, minor, and remission RAS was predominantly 65 kDa molecular weight."

"Kurkumin merupakan komponen utama yang terkandung dalam tumbuhan (Curcuma longa). Kurkumin diketahui memiliki beberapa sifat farmakologis. Salah satu khasiat farmakologis kurkumin adalah anti inflamasi yang diketahui dapat digunakan untuk mengobati stomatitis. Tumbuhan lain yang memiliki sifat farmakologis yang sama adalah Aloe vera, Aloe vera diketahui dapat memberikan efek penyembuhan pada stomatitis. Berdasarkan kemampuan dari Kurkumin dan Aloe vera, diharapkan dengan dikombinasikan antara keduanya maka dapat menjadi sebuah pilihan baru untuk terapi stomatitis yang lebih baik. Tujuan dari penelitian ini adalah untuk mengetahui efek anti stomatitis kombinasi zat aktif dan hasil formulasi dalam bentuk gel mukoadhesif dari kurkumin dan Aloe vera pada stomatitis yang diinduksi pada tikus. Model penelitian in vivo menggunakan 5 kelompok tikus dengan masing-masing 6 ekor tikus (Sprague Dawley). Semua tikus menerima aplikasi induksi asam asetat 50% pada mukosa rongga mulut. Pemberian secara topikal kombinasi zat aktif maupun hasil formula gel mukoadhesif yang mengandung Kurkumin-β-siklodekstrin dan Aloe vera (1:2) menunjukkan waktu penyembuhan yang lebih baik dibandingkan kombinasi lainnya. Hasil histopatologi juga menunjukkan bahwa kombinasi Kurkumin-β-siklodekstrin dan Aloe vera (1:2) memberikan efek penyembuhan yang baik. Kombinasi Kurkumin-β- siklodekstrin dan Aloe vera (1:2) maupun gel mukoadhesif yang mengandung Kurkumin- β-siklodekstrin dan Aloe vera (1:2) menunjukkan hasil yang positif untuk penyembuhan sariawan yang diinduksi oleh asam asetat 50%.

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Curcumin is the main component contained in plants (Curcuma longa). Curcumin is known to have several pharmacological properties. One of the pharmacological properties of curcumin is anti-inflammatory which is known to be used to treat stomatitis. Another plant that has the same pharmacological properties is Aloe vera. Aloe vera is known to have a healing effect on stomatitis. Based on the ability of curcumin and aloe vera, it is hoped that the combination of the two can become a new option for better stomatitis therapy. The purpose of this study was to determine the anti-stomatitis effect of the combination of active substances and the results of the formulation in the form of a mucoadhesive gel from curcumin and Aloe vera on induced stomatitis in rats. The in vivo research model used five groups of rats with six rats each (Sprague Dawley). All mice received an induction application of 50% acetic acid to the oral mucosa. Topical administration of a combination of active substances and the results of a mucoadhesive gel formula containing curcumin-β-cyclodextrin and aloe vera (1:2) showed a better healing time than other combinations. Histopathological results also showed that the combination of curcumin-β-cyclodextrin and aloe vera (1:2) gave an excellent healing effect. The combination of curcumin-β-cyclodextrin and aloe vera (1:2), as well as a mucoadhesive gel containing curcumin-β-cyclodextrin and aloe vera (1:2), showed positive results for the healing of stomatitis induced by 50% acetic acid."

"Recurrent Aphthous Ulceration (RAU) is a type of local inflammation of the oral mucosa with symptomatic soft tissue damage. The prevalence of RAU is about 17-67 %. Dominant factors causing this disease are understood, but there are predicted internal and external factors that cause related immune disorders. RAU is initiated by mucous proteins which continuously stimulate a physiological response required for a pathophysiological reaction. The aim of this study was to characterize specific anomaly proteins in oral mucosa as causing the initiation of RAU. Samples of mucosal proteins from 30 RAU patients were analyzed with sodium dodexylsulphate polyacrylamid gelelectrophorese (SDS-PAGE) and visualized with silver stain (AgNO3) showing proteins with a range of molecular weight 27 - 180 kDa. Western blotting using a polyclonal antibody specific to RAU showed that the specific proteins of RAU have molecular weights of 23, 27, 65, 70 and 87 kDa. The finding of so many proteins appears to be a new phenomenon, suggesting that the initiation of RAU is possibly due to a continuous induction of internal and external reactions by several mucosal proteins, that become anomaly proteins of high reactivity and antigenicity. This situation can cause overreaction on the oral mucosa with specific symptoms that are known as a RAU."

"Latar Belakang :Pesantren merupakan institusi pendidikan di Indonesia yang menjalankan sistem tempat tinggal asrama. Kondisi status kesehatan gigi mulut di beberapa pesantren masih menunjukkan hasil sedang hingga rendah, padahal terdapat lebih dari empat juta remaja yang menempuh pendidikan di pesantren. Upaya meningkatkan kesehatan gigi mulut adalah melaksanakan program promosi kesehatan mulut berbasis sekolah, dan program ini dapat disusun dengan sebelumnya melakukan identifikasi perilaku kebersihan gigi mulut.Tujuan : Menganalisis hubungan antara perilaku kebersihan gigi mulut dengan indeks plak, laju alir saliva, dan kuantifikasi bakteri Veillonella Parvula dalam saliva di komunitas pesantren populasi anak usia 12 – 14 tahun. Metode: Penelitian dilakukan pada 101 siswa Ibnu Hajar Boarding School. Pengisian kuesioner indeks OHB untuk menilai perilaku kebersihan gigi mulut. Pengambilan sampel saliva tanpa stimulasi dan diukur lajur alir, dilanjutkan pemeriksaan indeks plak. Sampel saliva dibawa ke laboratorium untuk mengetahui kuantifikasi bakteri Veillonella parvula melalui metode RT-PCR. Hasil: Koefisien korelasi antara OHB dengan Indeks plak adalah r = 0.127 p-value = 0.204. Koefisien korelasi antara OHB dengan laju alir saliva adalah r = -0.211, p-value = 0.034. Koefisien korelasi antara OHB dengan Ct Veillonella parvula adalah r = -0.156 , p-value = 0.119. Kesimpulan: Terdapat hubungan berbanding terbalik dan bermakna antara perilaku kebersihan gigi mulut dengan laju alir saliva, dan hubungan tidak bermakna antara perilaku kebersihan gigi mulut dengan indeks plak dan kuantifikasi bakteri Veillonella parvula.

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Background: Boarding schools in Indonesia operate as residential educational institutions. The oral health status in some boarding schools still indicates moderate to low results, despite more than four million adolescents pursuing education in these institutions. Efforts to improve oral health include implementing a school-based oral health promotion program, which can be designed after identifying oral hygiene behaviors. To date, there has been no study examining the relationship between oral hygiene behaviors and plaque index, saliva flow rate, and quantification of Veillonella Parvula. Objective: To analyze the relationship between oral hygiene behaviors and plaque index, saliva flow rate, and quantification of Veillonella Parvula in a population of 12- to 14-year-old students in a boarding school. Method: The OHB index questionnaire was used to assess oral hygiene behaviors. Unstimulated saliva samples were collected and saliva flow rate measured, followed by plaque index examination. Saliva samples were taken to the laboratory to determine the quantification of Veillonella Parvula bacteria using RT-PCR. Results: The correlation coefficient between OHB and the plaque index was r = 0.127, p-value = 0.204. The correlation coefficient between OHB and saliva flow rate was r = -0.211, p-value = 0.034. The correlation coefficient between OHB and Ct Veillonella Parvula was r = -0.156, p-value = 0.119. Conclusion: There was an inverse and significant relationship between oral hygiene behavior and salivary rate, and a non-significant relationship between oral hygiene behavior and plaque index and quantification of Veillonella parvula bacteria."