Hasil Pencarian  ::  Simpan CSV :: Kembali

"Chikungunya telah diidentifikasi di lebih dari 60 negara di Asia, Afrika, Eropa dan Amerika. Indonesia merupakan negara endemis chikungunya. Gejala yang ditimbulkan oleh chikungunya yaitu arthralgia yang dapat bertahan selama beberapa tahun. Senyawa α-mangostin ditemukan pada xanthone yang diambil dari perikarp buah manggis dan diketahui memiliki aktivitas antiviral terhadap virus Hepatitis C dan virus Dengue. Berdasarkan penyebaran virus yang cepat ke daerah baru, peningkatan jumlah penderita, dan potensi timbulnya cacat permanen, dibutuhkan agen antiviral terhadap infeksi CHIKV. Oleh karena itu, uji aktivitas antiviral α-mangostin terhadap virus chikungunya pada sel HepG2 dilakukan. Senyawa α-mangostin terlebih dahulu diuji toksisitasnya terhadap sel HepG2 dengan MTT assay. Selanjutnya dilakukan uji antiviral dengan konsentrasi α-mangostin sebesar 3,125 μM, 6,25 μM, dan 12,5 μM pada pre—treatment, full treatment, dan post—treatment. Supernatan sampel diambil dan titer virus diukur dengan metode plaque assay. Hasil uji toksisitas senyawa α-mangostin menunjukkan nilai CC50 sebesar 10,98 μM. Hasil uji antiviral menunjukkan adanya penurunan titer virus dalam concentration-dependent manner pada tiga perlakuan. Titer virus chikungunya berkurang lebih banyak pada full treatment (IC50 = 6,46 μM) dan post—treatment (IC50 = 6,99 μM) dibandingkan dengan pre—treatment (7,22 μM). Pemberian senyawa α-mangostin memiliki efek antiviral dengan menghambat replikasi CHIKV.

---

Chikungunya has been identified in more than 60 countries in Asia, Africa, Europe, and America. Indonesia is endemic country for chikungunya. The symptom is arthralgia which can last several years. α-mangostin is found in xanthones from mangosteen pericarp and is known to have antiviral activity against Hepatitis C virus and Dengue virus. Based on the rapid spread of virus to new areas, increasing number of sufferers, and potential cause of permanent disability, antiviral agents against CHIKV are needed. Antiviral activity test of α-mangostin against CHIKV on HepG2 cells was carried out. α-mangostin was tested for its toxicity against HepG2 cells by MTT assay. Furthermore, the antiviral test was carried out with 3,125 μM, 6,25 μM, and 12,5 μM α-mangostin in pre—treatment, full treatment, and post—treatment. The supernatant were taken and the virus titer was measured by plaque assay. Toxicity test of α-mangostin showed CC50 value of 10,98 μM. Antiviral test results showed decrease in virus titer (concentration-dependent manner). CHIKV titer reduction was more effective in full treatment (IC50 = 6,46 μM) and post—treatment (IC50 = 6,99 μM) compared to pre—treatment (IC50 = 7,22 μM). This suggests that α-mangostin has antiviral effect by inhibit CHIKV replication"

"[Angka kejadian penyakit mieloma multipel kecil, yaitu 0,8% di dunia dan 0,6% diAsia Tenggara dari seluruh kasus kanker yang ada. Namun, penyakit ini terjadisecara asimtomatik sehingga sulit didiagnosis, belum dapat disembuhkan, danmudah mempengaruhi organ dalam tubuh. Kulit buah manggis yang jarangdimanfaatkan diketahui mengandung senyawa xanton (polifenolat) yang memilikiaktivitas antikanker. Penelitian in vitro menggunakan sel jalur p3x63ag8 untukmenemukan ada tidaknya efek sitotoksisitas ekstrak etanol kulit buah manggis sertaIC50. Sel dibagi menjadi 9 kelompok, yaitu 1 kelompok kontrol dan 8 kelompokperlakuan dengan konsentrasi 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100μg/ml, 200 μg/ml, 400 μg/ml, dan 800 μg/ml. Data diambil dengan metode MTTassay dan hasilnya berupa nilai optical density. Setelah inkubasi 48 jammenggunakan ekstrak etanol kulit buah manggis, hasil persamaan garis diketahuiIC50 nya adalah 5,41 μg/ml. Analisis statistik dengan Kruskal Wallis menghasilkanadanya perbedaan efek sitotoksik pada konsentrasi yang berbeda . Uji Post Hocdidapatkan perbedaan bermakna antara kelompok kontrol dan kelompok perlakuan6,25 μg/ml dengan kelompok perlakuan lain.;Multiple myeloma disease has small incidence, namely 0,8% in the world and 0,6%in Southeast Asia of all cancer cases. However, the diasease occurs in asymptomaticthat so difficult to be diagnosed, can not be cured, and affects many organs. Themangosteen pericarp which rarely used evidently contain xanthone (polifenolat)compound which have anticancer activity. Research in in vitro manner using celllines p3x63ag8 to discover the presence of cytotoxicity effect of mangosteenpericarp ethanol extract and the IC50. Cells was divided into 9 groups, 1 controlgroup and 8 treatment groups (consentrations: 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml,50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml). Data taken by MTTassay method and the result is optical density value. After 48-hours incubationperiod and the result in line equation, found that IC50 was 5.41 ug / ml. Statisticalanalysis with Kruskal Wallis declared differences in the cytotoxic effects ofdifferent concentrations.Post Hoc test found significant difference beetwen thecontrol group and the treatment group of 6.25 ug / ml just than other groups;Multiple myeloma disease has small incidence, namely 0,8% in the world and 0,6%in Southeast Asia of all cancer cases. However, the diasease occurs in asymptomaticthat so difficult to be diagnosed, can not be cured, and affects many organs. Themangosteen pericarp which rarely used evidently contain xanthone (polifenolat)compound which have anticancer activity. Research in in vitro manner using celllines p3x63ag8 to discover the presence of cytotoxicity effect of mangosteenpericarp ethanol extract and the IC50. Cells was divided into 9 groups, 1 controlgroup and 8 treatment groups (consentrations: 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml,50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml). Data taken by MTTassay method and the result is optical density value. After 48-hours incubationperiod and the result in line equation, found that IC50 was 5.41 ug / ml. Statisticalanalysis with Kruskal Wallis declared differences in the cytotoxic effects ofdifferent concentrations.Post Hoc test found significant difference beetwen thecontrol group and the treatment group of 6.25 ug / ml just than other groups, Multiple myeloma disease has small incidence, namely 0,8% in the world and 0,6%in Southeast Asia of all cancer cases. However, the diasease occurs in asymptomaticthat so difficult to be diagnosed, can not be cured, and affects many organs. Themangosteen pericarp which rarely used evidently contain xanthone (polifenolat)compound which have anticancer activity. Research in in vitro manner using celllines p3x63ag8 to discover the presence of cytotoxicity effect of mangosteenpericarp ethanol extract and the IC50. Cells was divided into 9 groups, 1 controlgroup and 8 treatment groups (consentrations: 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml,50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml). Data taken by MTTassay method and the result is optical density value. After 48-hours incubationperiod and the result in line equation, found that IC50 was 5.41 ug / ml. Statisticalanalysis with Kruskal Wallis declared differences in the cytotoxic effects ofdifferent concentrations.Post Hoc test found significant difference beetwen thecontrol group and the treatment group of 6.25 ug / ml just than other groups]"

"[Buah manggis (Garcinia mangostana Linn) merupakan salah satu buah tropis dari Asia Tenggara seperti Indonesia dan kulitnya biasanya digunakan sebagai obat tradisional untuk mengatasi inflamasi dan mikroorganisme. Selain itu, kulit buah manggis juga diperkirakan dapat digunakan sebagai antikanker. Tujuan dari penelitian ini adalah mengetahui pengaruh ekstrak etanol kulit buah manggis terhadap viabilitas sel Raji secara in vitro melalui uji sitotoksisitas. Ekstrak etanol kulit buah manggis didapatkan melalui proses maserasi dan evaporasi dengan rotary evaporator. Ekstrak dibagi menjadi beberapa konsentrasi, yaitu 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, dan 800 μg/ml, kemudian diujikan ke sel Raji dan diinkubasi selama 48 jam. Uji sitotoksisitas yang digunakan adalah metode MTT-assay. Sifat sitotoksisitas ekstrak tersebut ditentukan oleh nilai IC50, lalu uji kemaknaan yang digunakan adalah Kruskal-Wallis. Hasil analisis menunjukkan nilai IC50 sebesar 3,07 μg/ml (p = 0,02). Kesimpulan dari penelitian ini adalah ekstrak etanol kulit buah manggis bersifat sitotoksik kuat terhadap viabilitas sel Raji dan ditemukan adanya perbedaan bermakna antar kelompok. Hasil uji Post Hoc memperlihatkan terdapat perbedaan bermakna antara kelompok kontrol dan kelompok perlakuan dengan konsentrasi 6,25 μg/ml dengan kelompok perlakuan lain.;Mangosteen (Garcinia mangostana Linn) is one of tropical fruit from south east Asia such as Indonesia and its pericarp usually used as traditional medicine for anti-inflammatory and anti-microorganism. Mangosteen pericarp is also expected can be used as anticancer. The aim of this study was to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on viability of Raji cells. The extract was obtained by maceration and evaporation process with rotary evaporator. The extract was divided into several concentration, such as 6.25 μg/ml, 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml, then it was tested with Raji cells and incubated during 48 hours. The cytotoxic effect against Raji cells is evaluated by MTT-assay. The cytotoxicity level of the extract is determined by IC50 value, then the significance test is used Kruskal-Wallis. The result of analysis showed that IC50 value was 3.07 μg/ml (p = 0.02). The conclusion of this research were the mangosteen pericarp ethanol extract has high cytotoxicity for viability Raji cells and there was a significant difference between groups. Post Hoc test result showed there were significant difference between control and 6.25 μg/ml group which compared with other groups;Mangosteen (Garcinia mangostana Linn) is one of tropical fruit from south east Asia such as Indonesia and its pericarp usually used as traditional medicine for anti-inflammatory and anti-microorganism. Mangosteen pericarp is also expected can be used as anticancer. The aim of this study was to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on viability of Raji cells. The extract was obtained by maceration and evaporation process with rotary evaporator. The extract was divided into several concentration, such as 6.25 μg/ml, 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml, then it was tested with Raji cells and incubated during 48 hours. The cytotoxic effect against Raji cells is evaluated by MTT-assay. The cytotoxicity level of the extract is determined by IC50 value, then the significance test is used Kruskal-Wallis. The result of analysis showed that IC50 value was 3.07 μg/ml (p = 0.02). The conclusion of this research were the mangosteen pericarp ethanol extract has high cytotoxicity for viability Raji cells and there was a significant difference between groups. Post Hoc test result showed there were significant difference between control and 6.25 μg/ml group which compared with other groups, Mangosteen (Garcinia mangostana Linn) is one of tropical fruit from south east Asia such as Indonesia and its pericarp usually used as traditional medicine for anti-inflammatory and anti-microorganism. Mangosteen pericarp is also expected can be used as anticancer. The aim of this study was to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on viability of Raji cells. The extract was obtained by maceration and evaporation process with rotary evaporator. The extract was divided into several concentration, such as 6.25 μg/ml, 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml, then it was tested with Raji cells and incubated during 48 hours. The cytotoxic effect against Raji cells is evaluated by MTT-assay. The cytotoxicity level of the extract is determined by IC50 value, then the significance test is used Kruskal-Wallis. The result of analysis showed that IC50 value was 3.07 μg/ml (p = 0.02). The conclusion of this research were the mangosteen pericarp ethanol extract has high cytotoxicity for viability Raji cells and there was a significant difference between groups. Post Hoc test result showed there were significant difference between control and 6.25 μg/ml group which compared with other groups]"

"Kulit buah manggis setelah diteliti ternyata mengandung beberapa senyawa dengan aktivitas farmakologi misalnya antiinflamasi antihistamin antibakteri antijamur dan antiviral Aktivitas antibakteri ekstrak kulit buah manggis dilaporkan memiliki uji antibakteri terhadap terhadap pseudomonas aeruginosa Pseudomonas aeruginosa merupakan bakteri gram negatif berflagel yang bersifat aerob Pseudomonas aeruginosatersebar luas di alam dan biasanya terdapat di lingkungan rumah sakit yang lembap Bakteri inibersifat invasif dan toksigenik menyebabkan infeksi pada pasien dengan daya tahan tubuh yang abnormal dan merupakan patogen nosokomial yang penting Pada penelitian ini uji aktivitas antibakteri Ekstrak kulit buah manggis Garcinia mangostana Linn menggunakanagar nutrient yang ditanami bakteri pseudomonas aeruginosa dan ditambahkan sumuran dengan Ekstrak sebagai subjek uji Uji yang digunakan adalah Ekstrak kulit buah manggis Garcinia mangostana Linn dengan pengenceran 10x 15x 20x 30x dan 40x yang dibandingkan dengan kontrol positif Eritromisin dan kontrol negatif Akuades Pengujian penghambatan pertumbuhan bakteridiukur denganmengukur zona bening disekitar sumuran dengan menggunakan jangka sorong dalam satuan mm terhadap seluruh sampel uji Hasil yang telah didapat dilakukan uji statistik Kruskal Wallis dengan post hoc Mann whitney didapatkan bahwa Ekstrak kulit buah manggis Garcinia mangostana Linn ternyata tidak mempunyai aktivitas antibakteri terhadap pseudomonas aeruginosa Terdapat hubungan yang tidak berbeda bermakna dengan kontrol negatif Akuades pada pengenceran Ekstrak 10x p 1 000 15x p 1 000 20x p 1 000 30x p 1 000 40x p 1 000 dan hubungan berbeda bermakna dengan kontrol positif Eritromisin pada pengenceran Ekstrak 10x p 0 013 15x p 0 013 20x p 0 013 30x p 0 013 40x p 0 013.

---

Mangosteen pericarp after investigation turned out to contain several compounds with pharmacological activity such as anti inflammatory antihistamine antibacterial antifungal and antiviral Antibacterial activity of mangosteen pericarp against pseudomonas aeruginosa is reported by antibacterial test ofmangosteen pericarp extract Pseudomonas aeruginosa is a gram negative bacteria have flagellaand that are aerobic Pseudomonas aeruginosa is widespread in nature and are usually found in the moist environment of the hospital These bacteria are invasive and toxigenic causing infections in patients with abnormal immune system and is an important nosocomial pathogen In this study the antibacterial activity test of mangosteen pericarp extract Garcinia mangostana Linn were examined with nutrient agar contain of pseudomonas aeruginosa culture and there are well filled of extract as test subjects Mangosteen pericarp extract used in this test is devided into a number of dilution 10x 15x 20x 30x and 40x that of compared with erythromycin as positive control and a distilled as negative control Testing of bacterial growth inhibition was measured by measuring the clear zone using a vernier caliper of all test samples The results obtained statistical by Kruskal Wallis test with post hoc Mann Whitney showed that extracts of mangosteen pericarp Garcinia mangostana Linn apparently did not have antibacterial activity against Pseudomonas aeruginosa There is no significant difference in relation to the negative control of distilled water at 10x extract dilution p 1 000 15x p 1 000 20x p 1 000 30x p 1 000 40x p 1 000 and different relationships erythromycin significantly with positive control at 10x extract dilution p 0 013 15x p 0 013 20x p 0 013 30x p 0 013 40x p 0 013 "

"Kulit buah manggis diketahui memiliki efek antibakteri, khususnya bakteri Propionibacterium acnes. Bakteri Propionibacterium acnes merupakan bakteri gram positif yang bersifat anaerob obligat. Bakteri ini merupakan flora normal pada kulit namun merupakan agen penyebab munculnya jerawat/acne vulgaris. Selain itu, infeksi P. acnes juga dapat menyebabkan sindrom SAPHO (synovitis, acne, pustulosis, hyperostosis, osteitis), osteomyelitis, infeksi gigi, rheumathoid arthritis, peritonistis, inflamasi prostat, sarkoidosis, dan infeksi yang berkaitan dengan alat seperti kateter, implan, dan lainnya. Resistensi pada bakteri P.acnes terhadap antibiotik juga merupakan masalah yang cukup penting di dunia yang berkaitan dengan pemakaian antibiotik yang tidak rasional. Pada penelitian ini aktivitas antibakteri ekstrak kulit buah manggis digunakan dengan Agar Brucella yang ditanami dengan bakteri dan ditambahkan sumuran dengan ekstrak sebagai uji. Uji yang digunakan adalah ekstrak kulit buah manggis dengan pengenceran 10 kali, 15 kali, 20 kali, 30 kali, dan 40 kali yang dibandingkan dengan kontrol negatif akuades serta kontrol positif tetrasiklin yang dibagi menjadi beberapa pengeceran yaitu 10 kali, 15 kali, 20 kali, 30 kali, dan 40kali. Hasil yang didapat kemudian dilakukan uji statistik menggunakan One Way Anova yang didapatkan bahwa ekstrak kulit buah manggis mempunyai aktivitas antibakteri hubungan yang berbeda bermakna dengan kontrol negatif pada pengenceran 10 kali (p<0.001), 15 kali (p<0.001), 20 kali (p<0.001), dan 30 kali (p<0.001), sedangkan ekstrak pengenceran 40 kali tidak mempunyai aktivitas antibakteri (p=1.000). Namun, ekstrak kulit buah manggis jika di bandingkan dengan antibiotik tetrasiklin mempunyai aktivitas yang lebih rendah.

---

Mangosteen pericarp is known to have antibacterial effects, especially against Propionibacterium acnes bacteria. Propionibacterium acnes is a gram-positive bacteria that are obligate anaerobes. These bacteria are normal flora of the skin but is a causative agent of pimples/acne vulgaris. In addition, P. acnes could also cause SAPHO syndrome (synovitis, acne, pustulosis, hyperostosis, osteitis), osteomyelitis, dental infections, arthritis rheumathoid, peritonistis, prostate inflammation, sarcoidosis, and infections associated with medical devices such as catheters, implants, and more. P. acnes resistance to antibiotics is also a significant problem in the world related to the irrational use of antibiotics. In this study, the antibacterial activity of mangosteen pericarp extract is examined with Brucella Agar in which there are well-filled of test solution such as extract, placebo, and/or positive control to show that it could inhibit the growth of P.acnes by measuring the inhibitory zone diameter.

The tests are using mangosteen pericarp extract with 10, 15, 20, 30, and 40 times dilution compared to the negative control and positive control tetracycline which is divided into a number of dilution that are 10x, 15x, 20x, 30x, and 40x. After the tests were measured by assessing the inhibitory zone diameter produced by each test. The results then performed statistical tests using One Way Anova showed that mangosteen pericarp extract has antibacterial activity with significantly different to the negative control at 10 times dilution (p<0.001), 15 times (p<0.001), 20 times (p<0.001), and 30 times (p<0.001), whereas 40 time dilution extract didn?t have antibacterial activity (p = 1.000). However, mangosteen pericarp extract has lower activity than tetracycline."

"[Kanker kolorektal merupakan keganasan ketiga terbanyak di Indonesia dengan95% diantaranya adalah adenokarsinoma kolon. Saat ini, tatalaksana yang dapatdiberikan berupa bedah reseksi atau laparoskopi masih terbatas khususnya padakanker kolon stadiur akhir. Sehingga, masih diperlukan penelitian untukmenemukan terapi alternatif untuk mendukung tatalaksana yang ada. Salahsatunya adalah kulit buah manggis yang dikatakan memiliki berbagai manfaat,termasuk antikanker. Ekstrak etanol kulit buah manggis (Garcinia mangostana l.)pada penelitian ini diuji efek sitotoksisitasnya terhadap sel adenokarsinoma kolon(C2BBE1) secara in vitro. Kulit manggis utuh segar dikeringkan, ditumbukmenjadi serbuk, dimaserasi dalam alkohol 99%, kemudian dievaporasi untukmenghasilkan crude extract kulit manggis. Dilakukan uji KLT dan fitokimiauntuk mengidentifikasi kandungan ekstrak. Digunakan delapan variasi konsentrasiekstrak, yaitu 6,2 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml,400 μg/ml, dan 800 μg/ml yang dilarutkan dalam DMSO dan media RPMIsebelum ditambahkan ke sel uji. Sel uji merupakan sel adenokarsinoma kolon dariDepartemen Patologi Anatomik FKUI/RSCM yang sebelum digunakan sudahditumbuhkan, diamati pertumbuhannya, dihitung kepadatannya, dan dipeliharadalam medium kultur komplit, pada suhu 37 °C dengan kandungan 5% CO2 padainkubator. Penambahan ekstrak dilakukan saat pertumbuhan sel konfluens dandiinkubasi kembali selama 48 jam untuk kemudian diamati di bawah mikroskopdino-eye dan dilakukan uji sitotoksisitas dengan metode MTT assay. Didapatkannilai %inhibisi proliferasi sel dengan pemberian ekstrak berbeda bermaknaterhadap kontrol dengan nilai p=0.015 (< 0,05) dengan uji Kruskal Wallis. NilaiIC50nya adalah 1,11 μg/ml yang berarti ekstrak yang mengandung polifenolat(termasuk xanton) tersebut bersifat sitotoksik kuat terhadap sel uji., Colorectal cancer is the third most found cancer in Indonesia in which 95% ofthem are colon adenocarcinoma. Today, the therapy is still limited in resectionsurgey or laparoscopy which is not efficient especially in late stadium. Therefore,alternative treatments are needed to support existing therapies. One of them isMangosteen Pericarp which is known for its many benefits including as ananticancer. In this study, mangosteen (Garcinia mangostana Linn) pericarpethanol extract’s cytotoxicity is tested on colon adenocarcinoma cells (typeC2BBE1). The fruit’s pericarp is peeled, dried, ground into powder, macerated in99% ethanol, then evaporated to create a crude extract of mangosteen pericarp.TLC and phytocemical screening is done to detect the components of the extract.There were 8 variations of extract concentration; 6.2 μg/ml, 12,5 μg/ml, 25 μg/ml,50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, and 800 μg/ml which were dissolvedin DMSO and RPMI media before given to tested cell lines. Tested cell lines wereavailable from anatomic pathology laboratory of FKUI RSCM which werecultured, monitored, counted, and inccubated in culture media under moistciurcumstances (370C, 5% CO2), The extracts are given to cell lines which were50% confluent then incubated for 48 hours. The cells then observed undermicroscope with dino-eye camera and tested using MTT assay kit to know thecytotoxycity. The results show significant difference between inhibitionpercentage of tested extracts to control with the value of p=0.015 (< 0,05)measured with Kruskal Wallis test. The IC50 value is 1.11μg/ml which means thatthe xanthon containing extract is highly cytotoxic to the tested cell lines]"

"[Kanker merupakan salah satu penyebab kematian utama di dunia, termasuk Indonesia. Berbagai penelitian dilakukan untuk mencari alternatif terapi kanker. Kulit manggis dipercaya mempunyai kandungan senyawa yang bersifat sitotoksik terhadap sel kanker. Penelitian ini bertujuan untuk mengetahui efek sitotoksisitas ekstrak etanol kulit manggis terhadap sel limfoma Hodgkin. Ekstrak yang digunakan berasal dari proses ekstraksi kulit manggis dengan pelarut etanol menggunakan Vaccum Rotary Evaporator pada tekanan 1 atm dengan suhu 60o C. Ekstrak kulit manggis diberikan dalam 8 konsentrasi berbeda yaitu 6,25 μg/ml, 12,5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml, 400 μg/ml, dan 800 μg/ml. Sitotoksisitas dinilai dengan uji MTT-assay untuk mendapat nilai IC50. Hasil penelitian menunjukkan bahwa ekstrak etanol kulit manggis mempunyai efek sitotoksik terhadap sel limfoma Hodgkin dengan nilai IC50 sebesar 5.6 μg/ml. Uji kemaknaan menggunakan uji Kruskal-Wallis menunjukkan nilai p = 0.008 (p ≤ 0.05). Kesimpulan dari penelitian ini yaitu ekstrak etanol kulit manggis mempunyai efek sitotoksik kuat terhadap sel Limfoma Hodgkin.;Cancer is one of the leading cause of death in the world, including Indonesia. Various studies have been done to seek alternative cancer therapy. Mangosteen pericarp is believed to have substance that are cytotoxic to cancer cells. The purpose of this study is to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on Hodgkin Lymphoma cells. The extract used in this study is obtained from the mangosteen pericarp extraction using Vacuum Rotary Evaporator at a pressure of 1 atm and temperature of 60o C. Mangosteen pericarp extract is given in eight different concentration of 6.25 ug / ml, 12.5 pg / ml, 25 mg / ml, 50 pg / ml, 100 pg / ml, 200 mg / mL, 400 mg / ml, and 800 ug / ml. Cytotoxicity was assessed using MTT-assay test to obtain IC50 values. The results showed that ethanol extract of mangosteen pericarp has a cytotoxic effect on Hodgkin lymphoma cells with IC50 value of 5.6 ug / ml. The data were analyzed using Kruskal-Wallis test and had a p value of 0.008 (p ≤ 0.05). The conclusion of this study is that ethanol extract of mangosteen pericarp has a strong cytotoxic effect on Hodgkin lymphoma cells, Cancer is one of the leading cause of death in the world, including Indonesia. Various studies have been done to seek alternative cancer therapy. Mangosteen pericarp is believed to have substance that are cytotoxic to cancer cells. The purpose of this study is to determine the in vitro cytotoxicity of mangosteen pericarp ethanol extract on Hodgkin Lymphoma cells. The extract used in this study is obtained from the mangosteen pericarp extraction using Vacuum Rotary Evaporator at a pressure of 1 atm and temperature of 60o C. Mangosteen pericarp extract is given in eight different concentration of 6.25 ug / ml, 12.5 pg / ml, 25 mg / ml, 50 pg / ml, 100 pg / ml, 200 mg / mL, 400 mg / ml, and 800 ug / ml. Cytotoxicity was assessed using MTT-assay test to obtain IC50 values. The results showed that ethanol extract of mangosteen pericarp has a cytotoxic effect on Hodgkin lymphoma cells with IC50 value of 5.6 ug / ml. The data were analyzed using Kruskal-Wallis test and had a p value of 0.008 (p ≤ 0.05). The conclusion of this study is that ethanol extract of mangosteen pericarp has a strong cytotoxic effect on Hodgkin lymphoma cells]"

"ABSTRACTInfeksi virus Dengue DENV merupakan salah satu masalah kesehatan di Indonesia yang hingga saat ini belum memiliki penanganan antivirus yang efektif. Tanaman Garcinia dulcis telah diketahui memiliki aktivitas antikanker, anti inflamasi, antimikroba maupun antivirus.Penelitian ini bertujuan untuk mengetahui aktivitas antivirus ekstrak daun tanaman Garcinia dulcis dalam menghambat replikasi virus Dengue serotipe 2 DENV-2 . Uji dilakukan secara in vitro pada sel Huh 7.5 terinfeksi DENV2 dengan multiplicity of infection 0.5 yang kemudian diberi ekstrak dalam berbagai konsentrasi 20 g/ml , 10 g/ml, 5 g/ml, 2,5 g/ml, dan 1,25 g/ml . Setiap kelompok perlakuan mendapat pengulangan sebanyak enam kali. Laju inhibisi replikasi DENV-2 dinilai melalui jumlah fokus virus yang terbentuksetelah proses immunostaining. Secara statistik, pemberian ekstrak daun Garcinia dulcis pada konsentrasi 20 g/ml , 10 g/ml, 5 g/ml, 2,5 g/ml menunjukkan penghambatan signifikan terhadap replikasi DENV2 p < 0,05 kecuali pada kelompok perlakuan ekstrak 1,25 g/ml p = 0,079 . Hambatan maksimum terlihat pada pemberian konsentrasi 20 g/ml dengan daya hambat replikasi sebesar 52,57 . Kata kunci: antivirus, Garcinia dulcis, virus Dengue.

---

ABSTRACTDengue Virus DENV infection remains a health problem in Indonesia without any specific antiviral treatment available yet. Garcinia dulcis has been known to have anticancer activity, antiinflamatory, antimicrobial activity, including antiviral. The aim of this research is to determine the antiviral activity of G.dulcis leaves extract in inhibiting the replication of DENV infection. This study conducted in vitro on Huh7.5 cellinfected by DENV2 with multiplicity of infection 0,5 followed by given the extract of G.dulcis in various concentrations 20 g ml, 10 g ml, 5 g ml, 2,5 g ml, 1,25 g ml . The treatment done in six time repetition to each groups. The inhibiton rate of DENV2 replication was assessed using focus assay after immunostaining process conducted. Treatment of G.dulcis leaves extract at concentration 20 g ml, 10 g ml, 5 g ml, 2,5 g ml shown a significant value inhibiting DENV2 replication p 0,05 , except the concentration of 1,25 g ml p 0,079 was insignificantly inhibits DENV2. Maximum inhibition shown at concentration of 20 g ml, which was inhibit 52,57 replication of DENV2. Keywords Antiviral, Dengue virus, Garcinia dulcis. "

"Pada kulit buah manggis (Garcinia mangostana L.) telah diketahui mengandung senyawa aktif berupa xanthone. Pada penelitian ini kandungan total fenolik, total flavonoid, aktivitas antioksidan dan uji sitotoksisitas dari fraksi etil asetat, n-butanol dan air dibandingkan dengan produk komersial obat ekstrak yang ditentukan dengan menggunakan metode spektrofotometri. Kandungan total fenolik di dalam hasil fraksinasi berkisar 1,69 – 15,87 mg ekivalen asam galat/g sampel fraksi, dinyatakan setara asam galat. Konsentrasi total flavonoid bervariasi 8,98 – 165,17 mg/ g ekstrak, dinyatakan setara quercetin. Aktivitas antioksidan dan uji sitotoksisitas fraksi etil asetat menunjukkan nilai sebesar 55.75 μg/mL dan 0.0029 μg/mL sebagai nilai IC50 dan LC50. Hasil analisis total fenolik dan total flavonoid menunjukkan nilai tertinggi pada sampel yang berasal dari fraksi etil asetat. Nilai IC50 dan LC50 menunjukkan bahwa sample dari fraksi etil asetat memiliki aktivitas antioksidan dan sitotoksisitas tertinggi dan terkuat. Hasil uji bioaktivitas maupun analisis fitokimia pada fraksi- fraksi yang mengandung xanthone ini dapat digunakan untuk menyeleksi sampel yang akan digunakan dalam pembuatan sistem pelepasan obat yang terkendali (controlled drug release).

---

Pericarp of mangosteen (Garcinia mangostana Linn) has been known one of the active compounds contained is Xanthone. In this study, total phenolic content, total flavonoid, antioxidant activity and cytotoxicity assay of fraction ethyl acetat, n-butanol and water compared to commercial product extracts in pericarp of mangosteen was determined using the spectrophotometric method.

The total phenolic content ranged from 1,69 - 15,87 mg/g extract, expressed as gallic acid equivalents. The total flavonoid concentrations varied from 8,98 - 165,17 mg/g extract, expressed as quercetin equivalents. Antioxidant activity and cytotoxicity assay ethyl acetat fraction showed a value of 55.75 μg/mL dan 0.0029 μg/mL were expressed as IC50 and LC50.

From the analysis it was found that ethyl acetate fraction showed the highest total phenolic content and flavonoid concentration also antioxidant activity and cytotoxicity assay ethyl acetate fraction a strong.

The test results on the bioactivity and phytochemical analysis of fractions containing xanthones can be used to select a sample that will be used in the manufacture of a controlled drug release."

"Kasus demam berdarah dengue DBD masih menjadi salah satu masalah kesehatan di dunia dan di Indonesia dengan tingginya angka kematian yang diakibatkan. Sampai saat ini belum terdapat terapi antiviral spesifik, sehingga terapi masih berupa suportif. Ekstrak daun Cynometra ramiflora Linn diketahui memiliki efek bakterisida, analgesik, antiviral, anti-inflamasi, dan anti-alergi. Kemampuan ekstrak daun Cynometra ramiflora Linn pada konsentrasi 20 ?g/ml , 10 ?g/ml, 5 ?g/ml, 2,5 ?g/ml, dan 1,25 ?g/ml sebagai anti-dengue virus DENV diujikan pada sistem in-vitro menggunakan sel Huh-7.5 terinfeksi DENV2 dengan multiplicity of infection moi 0,5. Kontrol positif dalam penelitian ini adalah sel Huh-7.5 yang terinfeksi DENV2, sel Huh-7.5 dengan pemberian pelarut dimethyl sulfoxide DMSO sebagai kontrol negaitf dan kontrol sel Huh-7.5 tanpa perlakuan, dengan enam ulangan pada setiap kelompok.. Efek hambat ekstrak terhadap replikasi DENV dinilai menggunakan metode foci-forming immunoassay. Secara statistik pemberian ekstrak daun Cynometra ramiflora Linn pada seluruh konsentrasi menunjukkan penghambatan signifikan terhadap replikasi DENV-2 p < 0,05 dibandingkan dengan kontrol positif. Tingkat penghambatan berturut-turut sebesar 36,06 , 45,96 , 47,35 , 55,94 , 62,70 pada konsentrasi 1,25 ?g/ml, 2,5 ?g/ml, 5 ?g/ml, 10 ?g/ml, dan 20 ?g/ml. Hasil penelitian ini menunjukkan ekstrak daun Cynometra ramiflora Linn berpotensi sebagai antiviral dengue.

---

Dengue hemorraghic fever DHF remains a major health problem of world particularly in Indonesia due to high moratlity rate of it. Until now, there is no specific antiviral therapy for DENV yet and the treatment is still supportive. The extract of Cynometra ramiflora Linn leaves known to have some effects such as bactericide, analgesic, antiviral, anti inflamation, and anti allergy. The potency Cynometra ramiflora Linn leaves extract at concentration of 1,25 g ml, 2,5 g ml, 5 g ml, 10 g ml, dan 20 g ml as anti viral dengue DENV was performed in vitro on Huh 7.5 cell infected by DENV 2 with MOI 0.5. Positive control in this research was Huh 7.5 cell infected by DENV 2, group of Huh 7.5 with dimethyl sulfoxide DMSO as negative control, and group of Huh 7.5 cell only as cell control. Each group was done in six repetition. The inhibition rate of the extract to DENV replication was measured using foci forming immunoassay. Statistically administration Cynometra ramiflora Linn leaves extract showed significant inhibition at each concentration p 0,05 compared with positive control. The inhibition rate were 36,06 , 45,96 , 47,35 , 55,94 , 62,70 at concentration of 1,25 g ml, 2,5 g ml, 5 g ml, 10 g ml, dan 20 g ml respectively. The result of this study showed that extract of Cynometra ramiflora Linn leaves has potency as antiviral dengue."