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"This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine.In this thesis, the author firstly describes the need and concept of partipetting for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications. "

"This book describes an effective framework for setting the right process parameters and new mold design to reduce the current plastic defects in injection molding. It presents a new approach for the optimization of injection molding process via (i) a new mold runner design which leads to 20 percent reduction in scrap rate, 2.5 percent reduction in manufacturing time, and easier ejection of injected part, (ii) a new mold gate design which leads to less plastic defects; and (iii) the introduction of a number of promising alternatives with high moldability indices. Besides presenting important developments of relevance academic research, the book also includes useful information for people working in the injection molding industry, especially in the green manufacturing field."

"This book presents innovative ideas and technical contributions in the area of metasurfaces and antenna technologies. On the one hand, it presents an effective method to analyze metasurfaces constituted by metallic texture with certain geometries. It shows how this method can be applied to the design of metasurface (MTS) antennas for deep space communications and other planar microwave devices. On the other hand, the book reports on a general methodology developed for analyzing flat devices realized by using modulated MTSs, which opens new design possibilities for a large number of microwave devices based on the manipulation of SWs. Finally, a novel approach of reconfigurability, which is based on a class of checkerboard MTS, is explored. All in all, this book covers important insights and significant results on the emerging topic of metasurfaces, from theoretical and computational aspects to experiments."

"This book describes an original improvement in power quality of photovoltaic generation systems obtained by the use of a multilevel inverter implemented with level doubling network (LDN). Modulation principles and harmonic analysis of output voltages are proposed and introduced in detail for both single and three-phase LDN configurations. The analysis is then extended to dc-link current and voltage, with emphasis to low-frequency harmonics and switching frequency ripple. This work represents the first comprehensive implementation of maximum power point tracking (MPPT) schemes using the ripple correlation control (RCC) algorithm in the presence of multiple ripple harmonics, such as in the case of multi­level inverters. Numerical simulations and experimental tests are carefully reported here, together with practical insights into the design of dc-link capacitors."

"Teknologi microfluidics untuk tujuan terapi telah berkembang khususnya dalam bidang drug delivery. Double emulsion droplet mampu memenuhi kebutuhan drug delivery karena terdiri dari geometry shell and core yang mana mampu melindungi drug yang terdapat di dalam shell. Perkembangan drug delivery bergantung pada geometri microchannel dan parameter kontrol laju aliran yang mana membutuhkan riset lebih lanjut untuk memenuhi kebutuhan industri. Kebutuhan industri saat ini adalah untuk membentuk double emulsion droplet yang mampu memenuhi dua karakteristik droplet, yaitu tingkat monodispersity dan high-throughput. Kemudian, modified flow-focusing device dengan injection model sebelum junction pertama dan sudden expansion model pada junction kedua dipertimbangan untuk dikembangkan dalam riset ini. Mengingat terdapat kesulitan dalam proses manufaktur dan keterbatasan riset dalam penelitian eksperimental untuk menentukan kualitas dan channel yang bervariasi, CFD (Computational Fluid Dynamics) dipertimbangkan untuk menganalisis performa device. Lebih lanjut, VOF (Volume of Fluid) diimplementasikan dalam penelitian ini untuk mendapatkan pengetahuan mendalam dalam fenomena aliran multifasa karena masih sedikit riset yang menganalisis dengan model tersebut khususnya untukk menganalisis pembentukkan double emulsion droplet. Kemudian, adanya kompleksitas dalam penentukan flow rate control parameter untuk menentukan solusi terbaik yang mana menentukan kualitas double emulsion droplet generation. Tujuan dalam riset ini adalah untuk menganalisis modified flow-focusing device, memvisualisasikan evolusi droplet dan evolusi tekanan selama proses, memetakan flow regime, dan analisis performa untuk membentuk produk double emulsion yang cocok untuk aplikasi drug delivery. Riset ini menggunakan software Design Expert untuk menentukan jumlah run minimal yang dibutuhkan dalam simulasi untuk mendapatkan hasil yang signifikan. Software ANSYS Fluent untuk simulasi CFD yang mana memungkinkan untuk memvisualisasikan evolusi droplet dan tekanannya. Software ImageJ untuk analisis statistik gambar untuk memeroleh diameter rata-rata droplet, coefficient of variation (CV) dan droplet generation rate. TOPSIS untuk menentukan solusi terbaik dari parameter kontrol laju aliran. Berdasarkan hasil analisis, modified flow-focusing device yang dilengkapi dengan injection model dan sudden expansion model dapat memastikan kestabilan dalam pemebentukkan double emulsion droplet. Hasil CFD menunjukkan bahwa terdapat dua flow regime yang diamati, yaitu dripping dan narrowing jetting. Evolusi droplet dapat menyediakan fenomena dua dripping instabilities yang terjadi selama mekanisme break-up dalam pembentukkan droplet. Kemudian, flow regime map menunjukkan metode kontrol flow regime untuk memproduksi double emulsion droplet untuk kebutuhan drug delivery. Parameter kontrol laju aliran terbaik yang menenuhi tingkat monodispersity dan high-throughput yang dibutuhkan dapat diperoleh menggunakan TOPSIS, yaitu droplet yang memiliki diameter outer droplet rata-rata sebesar 34.51 μm dan diameter inner droplet rata-rata sebesar 14.76 μm, kemudian CV (Coefficient of Variation) dari outer droplet sebesar 1.95% dan CV dari inner droplet sebesar 2.34% yang memenuhi kebutuhan monodispersity, serta frekuensi pembentukkan droplet sebesar 818.18 Hz untuk kedua outer dan inner droplet yang memenuhi high-throughput. Hasil ini diperoleh menggunakan kombinasi dari 2.3 μL/h inner phase flow rate, 52.4 μL/h middle phase flow rate, and 348.3 μL/h outer phase flow rate dan memiliki potensi untuk drug delivery khususnya dalam pemenuhan kebutuhan di industri.

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Microfluidics technology for therapeutics purpose has further developed especially in the drug delivery. The double emulsion droplet satisfies the drug delivery since it consists of core and shell geometry which can protect the drug inside the shell. Drug delivery development depends on microchannel geometry and flow rate control parameter which needs follow up research to fulfil industrial need. The industrial current need is to generate double emulsion droplet which meets both high-throughput and monodispersity. Then, modified flow-focusing device with an injection model before first junction and sudden expansion model at second junction is considered in this research. Because of difficulty in manufacturing and limitation in experimental for determining the quality of various channels, CFD (Computational Fluid Dynamics) is considered to analyze the device performance. Furthermore, the VOF (Volume of Fluid) is implemented to obtain insights in the multiphase flow phenomenon since this model lacks research especially to analyze the double emulsion droplet generation. Then, the complexity in determination of flow rate control parameter leads to find the best solution which prescribes quality of double emulsion droplet generation. The research aims to analyze the modified flow-focusing microfluidics device, visualizing droplets evolution and pressure evolution during the process, mapping the flow regime, and performance analysis to generate a double emulsion product suitable for drug delivery application. The research utilizes Design Expert software to determine number of simulations run required to obtain significant result. ANSYS Fluent is utilized for CFD simulation which enables to visualize droplet and pressure evolution. ImageJ is used for image statistical analysis to gain average droplet diameter, coefficient of variation (CV) and droplet generation rate. TOPSIS for choosing the best solution of flow rate control parameter. Based on the analysis, the modified flow-focusing device equipped with the injection model and sudden expansion can ensure the stability in double emulsion droplet generation. The CFD result shows that there are two flow regimes observed, that is dripping and narrowing jetting. The droplet evolution can provide two dripping instability occurs during the break-up mechanism in droplet generation. Then, the flow regime map depicts the way to control the flow regime to produce double emulsion droplet for drug delivery. The best flow rate control parameter which satisfies both high-throughput and monodispersed need is obtained using TOPSIS, that is 34.51 μm outer droplet and 14.76 μm inner droplet for average droplet diameter, then 1.95% outer droplet CV and 2.34% inner droplet CV for satisfying the monodispersity, and 818.18 Hz of both outer and inner droplet generation rate satisfying the high-throughput. This result can be achieved using combination of 2.3 μL/h inner phase flow rate, 52.4 μL/h middle phase flow rate, and 348.3 μL/h outer phase flow rate and potential to satisfy the drug delivery in accordance with the industrial need."

"Next generation sequencing is revolutionizing molecular biology. Owing to this new technology it is now possible to carry out a panoply of experiments at an unprecedented low cost and high speed. These go from sequencing whole genomes, transcriptomes and small non-coding RNAs to description of methylated regions, identification protein – DNA interaction sites and detection of structural variation. The generation of gigabases of sequence information for each of this huge bandwidth of applications in just a few days makes the development of bioinformatics applications for next generation sequencing data analysis as urgent as challenging."

"Backed by leading authorities, this is a professional guide to successful compound screening in pharmaceutical research and chemical biology, including the chemoinformatic tools needed for correct data evaluation. The authors discuss such factors as chemical genetics, binding, cell-based and biochemical assays, the efficient use of compound libraries and data mining using cell-based assay results."