Hasil Pencarian  ::  Simpan CSV :: Kembali

"ABSTRAKHelicobacter pylori diperkirakan menginfeksi lebih dari setengah populasi orang dewasa. Deteksi dini H.pylori sangat diperlukan untuk mencegah berkembangnya infeksi menjadi keganasan lambung. Bentuk coccoid dari H. pylori sulit dideteksi dengan kultur dan histopatologi, namun dapat terdeteksi dengan metode molekuler seperti real-time PCR. Salah satu gen yang dapat digunakan sebagai gen target real-time PCR yaitu 16S rRNA, yang diketahui spesifik dan juga digunakan untuk menganalisis kekerabatan antar strain. Analisis ini bermanfaat untuk melihat penyebaran infeksi H.pylori di dunia. Penelitian ini merupakan penelitian eksperimental laboratorium. Metode pengambilan sampel yang digunakan yaitu, metode consecutive sampling. Biopsi diambil dari 2 antrum dan 2 korpus pada 42 penderita dispepsia untuk pemeriksaan real-time PCR dan histopatologi. Optimasi kondisi real-time PCR meliputi uji volume cetakan DNA, sensitifitas dan spesifisitas teknik, kemudian dilanjutkan aplikasi pada sampel klinis dari biopsi lambung. Delapan dari 11 sampel yang positif dilakukan sekuensing dan analisis filogenetik. Hasil optimasi diperoleh suhu annealing 64?C, konsentrasi primer 0,8 ?M dan konsentrasi probe 0,6 ?M. Ambang batas deteksi real-time PCR untuk mendeteksi jumlah DNA minimal H.pylori yaitu 46 bakteri. Spesifisitas uji reaksi silang real-time PCR ini tidak menunjukkan adanya reaksi silang dengan mikroorganisme lain. Proporsi positif hasil pemeriksaan real-time PCR sebesar 26,2 , sedangkan histopatologi sebesar 11,9 . Pemeriksaan real-time PCR mampu meningkatkan diagnosis sebesar 14,3 dibandingkan pemeriksaan histopatologi. Hasil sekuensing dan analisis filogenetik menunjukkan bahwa strain H.pylori dari sampel memiliki kekerabatan dengan strain Taiwan, India, dan Australia. Kata kunci : H.pylori, histopatologi, real-time PCR, analisis filogenetik

---

ABSTRACTHelicobacter pylori infection is estimated infect almost half of the adult population in the world. Early detection of H.pylori is needed to prevent the development of infections into gastric malignancies. The coccoid form of H. pylori is difficult to detect using culture and histopathology but it can be detected by molecular methods such as real time PCR. One of the genes that can be used as a real time PCR target gene is 16S rRNA, which is known to be specific and also used to analyze closely related strain. This analysis were useful to showed the spread of H.pylori infection in the world.This study is an experimental laboratory. The sampling method used is the consecutive sampling method. Biopsy was taken from 2 antrum and 2 corpus in 42 patients with dyspepsia for real time PCR and histopathology examination. Optimization real time PCR conditions include DNA template volume testing, sensitivity and specificity of the technique, followed by application of clinical samples from gastric biopsy. Eight of the 11 positive samples were sequenced and analyzed for phylogenetics pattern. The optimization result obtained annealing temperature 64 C, primer concentration was 0,8 M and probe concentration was 0,6 M. Limit detection of the DNA was 46 bacteria. The specificity of the PCR 39 s real time indicate that there was no cross reaction with other microorganisms. The positive proportion of PCR real time examination was 26.2 , while histopathology was 11.9 . A real time PCR examination was able to improve the diagnosis by 14,3 compared to histopathology examination. Sequencing and phylogenetic analysis results showed that our strain were closely related to Taiwan, India and Australia strains. Keywords H.pylori, histopathology, real time PCR, phylogenetic analysis"

"Latar Belakang: Diagnosis definitif Helicobacter pylori H.pylori hingga kini masih merupakan masalah. Biakan untuk isolasi dan identifikasi bakteri ini sulit. Uji cepat urease direkomendasikan sebagai uji diagnostik lini pertama pasien dispepsia. Tujuan: Mengembangkan komposisi medium biakan dan deteksi cepat H.pylori pada spesimen biopsi lambung pasien dispepsia. Metode: Desain penelitian merupakan studi potong lintang dan eksperimental laboratorium. Sampel diambil dengan cara consecutive sampling sebanyak 68 spesimen biopsi lambung 34 antrum, 34 korpus, masing-masing untuk biakan dan uji MIU. Sebagai pembanding digunakan histopatologi dan PCR. Mula-mula dilakukan optimasi medium biakan dan MIU konsentrasi merah fenol, pH, urea dan suhu inkubasi. Selanjutnya kondisi optimal yang diperoleh diaplikasikan pada spesimen biopsi pasien dispepsia. Hasil: Medium biakan agar darah Columbia ditambah vankomisin 5 mg / 500 mL dan darah domba 7 belum optimal, namun dapat digunakan untuk isolasi dan identifikasi. Hasil MIU modifikasi sebagai berikut: konsentrasi merah fenol 0,001 ; urea 4 ; pH medium 7; Suhu inkubasi optimal 35-370 C. Proporsi positif hasil uji MIU sebesar 35,29 12/34, biakan 32,35 11/34, PCR 32,35 11/34 dan histopatologi 20,59 7/34. Kesimpulan: Pemeriksaan MIU meningkatkan positivitas hasil pemeriksaan sebesar 14,7 bila dibandingkan dengan histopatologi.

---

Background: Until now, definitive diagnostic of H.pylori is still a problem. Culture for isolation and identification of this pathogen is difficult. Rapid urease test is recommended as a first line diagnostic test.

Aim: To obtain optimal composition for culture medium and Motility Indol Urease MIU test for the detection of H. pylori in dyspeptic patient biopsy specimens.

Method: A cross sectional and experimental laboratory study was performed. Sixty eight gastric biopsy samples 34 antrum, 34 corpus were collected by consecutive sampling method for culture and MIU test. Histopathology and PCR were conducted for comparison. Initially, we performed the optimation of culture medium and MIU test phenol red and urea concentration, pH, and temperature. The optimal condition obtained was then applied to the specimens.

Result: Columbia agar supplemented with vancomycin 5 mg 500 mL and 7 sheep blood was unable to create an optimal condition, but it can be used for isolation and identification. Modified MIU was performed by this following condition phenol red 0,001 urea 4 pH 7 incubation temperature 35 37oC. Positive proportion of MIU was 35.29 12 34, culture 32.35 11 34, PCR 32,35 11 34 and histopathology 20.59 7 34.

Conclusion: MIU test was able to improve the positivity rate by 14,7 compared to histopathology."

"Latar Belakang: Diagnosis definitif Helicobacter pylori (H.pylori) hingga kini masih merupakan masalah. Biakan untuk isolasi dan identifikasi bakteri ini sulit. Uji cepat urease direkomendasikan sebagai uji diagnostik lini pertama pasien dispepsia. Tujuan: Mengembangkan komposisi medium biakan dan deteksi cepat H.pylori pada spesimen biopsi lambung pasien dispepsia. Metode: Desain penelitian merupakan studi potong lintang dan eksperimental laboratorium. Sampel diambil dengan cara consecutive sampling sebanyak 68 spesimen biopsi lambung (34 antrum, 34 korpus), masing-masing untuk biakan dan uji MIU. Sebagai pembanding digunakan histopatologi dan PCR. Mula-mula dilakukan optimasi medium biakan dan MIU (konsentrasi merah fenol, pH, urea dan suhu inkubasi). Selanjutnya kondisi optimal yang diperoleh diaplikasikan pada spesimen biopsi pasien dispepsia. Hasil: Medium biakan agar darah Columbia ditambah vankomisin 5 mg/500 mL dan darah domba 7% belum optimal, namun dapat digunakan untuk isolasi dan identifikasi. Hasil MIU modifikasi sebagai berikut: konsentrasi merah fenol 0,001%; urea 4%; pH medium 7; Suhu inkubasi optimal 35-370 C. Proporsi positif hasil uji MIU sebesar 35,29% (12/34), biakan 32,35%(11/34), PCR 32,35%(11/34) dan histopatologi 20,59% (7/34). Kesimpulan: Pemeriksaan MIU meningkatkan positivitas hasil pemeriksaan sebesar 14,7% bila dibandingkan dengan histopatologi.

---

Background: Until now, definitive diagnostic of H.pylori is still a problem. Culture for isolation and identification of this pathogen is difficult. Rapid urease test is recommended as a first-line diagnostic test. Aim: to obtain optimal composition for culture medium and Motility-Indol-Urease (MIU) test for the detection of H. pylori in dyspeptic patient biopsy specimens. Method: A cross sectional and experimental laboratory study was performed. Sixty eight gastric biopsy samples (34 antrum, 34 corpus) were collected by consecutive sampling method for culture and MIU test. Histopathology and PCR were conducted for comparison. Initially, we performed the optimation of culture medium and MIU test (phenol red and urea concentration, pH, and temperature). The optimal condition obtained was then applied to the specimens. Result: Columbia agar supplemented with vancomycin 5 mg / 500 mL and 7% sheep blood was unable to create an optimal condition, but it can be used for isolation and identification. Modified MIU was performed by this following condition: phenol red 0,001%; urea 4%; pH 7; incubation temperature 35-37oC. Positive proportion of MIU was 35.29% (12/34), culture 32.35% (11/34), PCR 32,35% (11/34) and histopathology 20.59% (7/34). Conclusion: MIU test was able to improve the positivity rate by 14,7% compared to histopathology. "

"

Latar belakang: Prevalensi infeksi Helicobacter pylori pada anak di Indonesia 8%- 52%. Gejala dominan pada anak dengan infeksi H. pylori adalah refluks gastroesofageal yang mengganggu kualitas hidup (penyakit refluks gastroesofageal/PRGE), yang secara definitf di diagnosis dengan pemeriksaan esofagogastroduodenoskopi (EGD). Untuk mengetahui infeksi dilakukan uji Rapid Urease Test (RUT) pada saat bedside, namun uji ini belum diketahui akurasinya Tujuan: Mendapatkan proporsi positif RUT pada biopsi lambung dibandingkan real-time PCR. Selain itu ingin diketahui karakteristik gambaran klinis, demografi, dan hubungan faktor risiko pada anak PRGE yang menjalani prosedur EGD. Metode: Penelitian potong lintang pada 46 anak dengan PRGE di RSCM dan RS MMC. Semua subyek menjalani RUT, real-time PCR dan histopatologi. Hasil: Anak perempuan berusia lebih dari 10-18 tahun dengan tingkat pendidikan orangtua rendah mendominasi karakteristik subyek penelitian ini. Nyeri perut lebih dari 3 bulan, anemia, status nutrisi, orangtua dispepsia dan kepadatan kapling rumah pada penelitian ini tidak terbukti sebagai faktor risiko terhadap terjadinya PRGE. Namun, pola makan tidak teratur dan komsumsi makan berempah memengaruhi terjadinya gastropati pada lambung anak (p < 0,05). Proporsi positif RUT; 2,2% dan real-time PCR; 8,7%. Kesimpulan: Hasil negatif pada pemeriksaan RUT tidak menyingkirkan terjadinya infeksi H. pylori, terutama pada pasien dalam terapi proton pump inhibitor (PPI). Pemeriksaan lanjutan menggunakan real-time PCR dianjurkan untuk mendukung diagnosis ini.

 

---

Background: The prevalence of detected Helicobacter pylori infection of children in Indonesia was 8%-52%. Gastroesophageal reflux was the dominant symptom and might be attributable to H. pylori infection which reduced quality of life. Current definitive diagnosis was using esophagogastroduodenoscopy (EGD). Rapid Urease Test (RUT) was used in bedside setting for H. pylori detection, however its accuracy was still unkown. Objectives: This study was done to determine the positive proportion of RUT on gastric biopsy specimens and real-time PCR. Moreover, this study explored the characteristics of clinical and demographic features, and examined the risk factors in children with GERD (gastroesophageal reflux disease) who underwent diagnostic EGD. Methods: This is a cross-sectional study on 46 children diagnosed as GERD, admitted to the RSCM and MMC Hospital. All subject underwent RUT, real-time PCR and histopathology examination. Results: Most subjects are girls, more than 10-18 years with low parental education dominated the proportion of subject included in this study. According to abdominal pain more than 3 months, anemia, nutritional status, parental dyspepsia and crowded household were not proven to be risk factors for increase of GERD. However, irregular feeding habit and consumption of spicy foods were be associated with gastropathy in child’s gastric mucosa (p < 0,05). The positive proportion of RUT was 2.2% and real-time PCR was 8.7%. Conclusion: The negative result of RUT could not rule out of H. pylori infection, especially in patients with proton pump inhibitor (PPI) therapy. Further examination using real-time PCR is needed to support the diagnosis.

 

"

"[ABSTRAKLatar Belakang : Risiko infeksi Helicobacter pylori (H.pylori) dikaitkan denganbanyak faktor yang terkait dengan pejamu-agen-lingkungan. Etnis adalah salahsatu faktor dari pejamu yang banyak diteliti di luar negeri. Prevalensi infeksiH.pylori didapatkan lebih tinggi pada etnis tertentu seperti misalnya di China.Berdasarkan teori Migrasi dan teori Transmisi diduga infeksi H.pylori akandibawa oleh penduduk yang bermigrasi dari daerah dengan prevalensi tinggi ketempat tujuan migrasi. Etnis Tionghoa di Kalimantan Barat berasal dari daerahChina Selatan dimana prevalensi infeksi H.pylori tinggi. Diperkirakan adaperbedaan angka prevalensi infeksi H.pylori pada etnis Tionghoa dibandingkanpada etnis asli Kalimantan Barat, yaitu etnis Dayak.Tujuan : Untuk mengetahui perbedaan angka prevalensi, karakteristikepidemiologis dan gambaran hasil pemeriksaan endoskopi pada subjek etnisTionghoa dan Dayak dengan sindrom dispepsia.Metode : Penelitian ini adalah studi potong lintang untuk mengetahui perbedaanangka prevalensi infeksi H.pylori. Penelitian dilakukan di RSU Santo AntoniusPontianak dari bulan Desember 2014 sampai Juni 2015 dengan metodepengambilan sampel secara consecutive sampling. Angka prevalensi infeksiH.pylori disajikan dalam angka persentase, sedangkan perbedaan karakteristikepidemiologis dan perbedaan gambaran hasil pemeriksaan endoskopi pada etnisTionghoa dan Dayak dianalisis dengan analisis bivariat menggunakan chi-squaredengan tingkat kemaknaan (p) = 0,05.Hasil : Dari 203 subjek yang diteliti, terdiri dari 102 subjek etnis Tionghoa dan101 subjek etnis Dayak didapatkan angka prevalensi H.pylori sebesar 40,8%.Prevalensi pada etnis Tionghoa didapatkan lebih tinggi dibanding etnis Dayak,berturut-turut sebesar 48,0% dan 33,7%. Tidak ada perbedaan karakteristikepidemiologis dan temuan hasil pemeriksaan endoskopi pada kedua kelompoketnis.Simpulan : Terdapat perbedaan angka prevalensi infeksi H.pylori pada etnisTionghoa (48,0%) dibanding etnis Dayak (33,7%). Tidak ada perbedaankarakteristik epidemiologis dan gambaran hasil pemeriksaan endoskopi padakedua kelompok etnis.

---

ABSTRACTBackground : Helicobacter pylori (H. pylori) infection risk is associated withmany factors related to host-agent-environment matter. Ethnicity is one of the hostfactors which was the most studied factor overseas. The prevalence of H. pyloriinfection was found higher in certain ethnic such among Chinese. Based onmigration and transmission theory, it was suspected that H. pylori infection weretransmitted by people migrating from areas with a high prevalence of infection tothe destination area. Chinese in West Borneo are originated from South Chinaregion where the prevalence of H. pylori infection is high. It is estimated thatthere are differences in the prevalence of H. pylori infection among Chinesecompared to the native people of West Borneo, the Dayaknese.Objective: To determine the differences in the prevalence, epidemiologicalcharacteristics and endoscopic finding between Chinese and Dayaknese withdyspeptic syndrome.Methods: This study was a cross-sectional study to determine the differences inthe prevalence of H. pylori infection. The study was conducted at St. AntoniusGeneral Hospital Pontianak from December 2014 to June 2015 with consecutivesampling method. H.pylori infection prevalence is presented in percentagenumbers, while the epidemiological characteristics and endoscopic findingdifferences among Chinese and Dayaknese were analyzed by bivariate analysisusing the chi-square with significance value (p) = 0.05.Results : From a total of 203 subjects in this study, consisted of 102 Chinesesubjects and 101 Dayaknese subjects, the prevalence of H.pylori infection was40.8%. The prevalence among Chinese is higher than Dayaknese, which is 48.0%and 33.7%, respectively. There is no difference in the epidemiologicalcharacteristics and endoscopic findings in both ethnic groups.Conclusions : The prevalence of H. pylori infection among the Chinese (48.0%)is higher than among Dayaknese (33.7%). There is no difference in theepidemiological characteristics and endoscopic finding among both ethnic groups.;Background : Helicobacter pylori (H. pylori) infection risk is associated withmany factors related to host-agent-environment matter. Ethnicity is one of the hostfactors which was the most studied factor overseas. The prevalence of H. pyloriinfection was found higher in certain ethnic such among Chinese. Based onmigration and transmission theory, it was suspected that H. pylori infection weretransmitted by people migrating from areas with a high prevalence of infection tothe destination area. Chinese in West Borneo are originated from South Chinaregion where the prevalence of H. pylori infection is high. It is estimated thatthere are differences in the prevalence of H. pylori infection among Chinesecompared to the native people of West Borneo, the Dayaknese.Objective: To determine the differences in the prevalence, epidemiologicalcharacteristics and endoscopic finding between Chinese and Dayaknese withdyspeptic syndrome.Methods: This study was a cross-sectional study to determine the differences inthe prevalence of H. pylori infection. The study was conducted at St. AntoniusGeneral Hospital Pontianak from December 2014 to June 2015 with consecutivesampling method. H.pylori infection prevalence is presented in percentagenumbers, while the epidemiological characteristics and endoscopic findingdifferences among Chinese and Dayaknese were analyzed by bivariate analysisusing the chi-square with significance value (p) = 0.05.Results : From a total of 203 subjects in this study, consisted of 102 Chinesesubjects and 101 Dayaknese subjects, the prevalence of H.pylori infection was40.8%. The prevalence among Chinese is higher than Dayaknese, which is 48.0%and 33.7%, respectively. There is no difference in the epidemiologicalcharacteristics and endoscopic findings in both ethnic groups.Conclusions : The prevalence of H. pylori infection among the Chinese (48.0%)is higher than among Dayaknese (33.7%). There is no difference in theepidemiological characteristics and endoscopic finding among both ethnic groups.;Background : Helicobacter pylori (H. pylori) infection risk is associated withmany factors related to host-agent-environment matter. Ethnicity is one of the hostfactors which was the most studied factor overseas. The prevalence of H. pyloriinfection was found higher in certain ethnic such among Chinese. Based onmigration and transmission theory, it was suspected that H. pylori infection weretransmitted by people migrating from areas with a high prevalence of infection tothe destination area. Chinese in West Borneo are originated from South Chinaregion where the prevalence of H. pylori infection is high. It is estimated thatthere are differences in the prevalence of H. pylori infection among Chinesecompared to the native people of West Borneo, the Dayaknese.Objective: To determine the differences in the prevalence, epidemiologicalcharacteristics and endoscopic finding between Chinese and Dayaknese withdyspeptic syndrome.Methods: This study was a cross-sectional study to determine the differences inthe prevalence of H. pylori infection. The study was conducted at St. AntoniusGeneral Hospital Pontianak from December 2014 to June 2015 with consecutivesampling method. H.pylori infection prevalence is presented in percentagenumbers, while the epidemiological characteristics and endoscopic findingdifferences among Chinese and Dayaknese were analyzed by bivariate analysisusing the chi-square with significance value (p) = 0.05.Results : From a total of 203 subjects in this study, consisted of 102 Chinesesubjects and 101 Dayaknese subjects, the prevalence of H.pylori infection was40.8%. The prevalence among Chinese is higher than Dayaknese, which is 48.0%and 33.7%, respectively. There is no difference in the epidemiologicalcharacteristics and endoscopic findings in both ethnic groups.Conclusions : The prevalence of H. pylori infection among the Chinese (48.0%)is higher than among Dayaknese (33.7%). There is no difference in theepidemiological characteristics and endoscopic finding among both ethnic groups., Background : Helicobacter pylori (H. pylori) infection risk is associated withmany factors related to host-agent-environment matter. Ethnicity is one of the hostfactors which was the most studied factor overseas. The prevalence of H. pyloriinfection was found higher in certain ethnic such among Chinese. Based onmigration and transmission theory, it was suspected that H. pylori infection weretransmitted by people migrating from areas with a high prevalence of infection tothe destination area. Chinese in West Borneo are originated from South Chinaregion where the prevalence of H. pylori infection is high. It is estimated thatthere are differences in the prevalence of H. pylori infection among Chinesecompared to the native people of West Borneo, the Dayaknese.Objective: To determine the differences in the prevalence, epidemiologicalcharacteristics and endoscopic finding between Chinese and Dayaknese withdyspeptic syndrome.Methods: This study was a cross-sectional study to determine the differences inthe prevalence of H. pylori infection. The study was conducted at St. AntoniusGeneral Hospital Pontianak from December 2014 to June 2015 with consecutivesampling method. H.pylori infection prevalence is presented in percentagenumbers, while the epidemiological characteristics and endoscopic findingdifferences among Chinese and Dayaknese were analyzed by bivariate analysisusing the chi-square with significance value (p) = 0.05.Results : From a total of 203 subjects in this study, consisted of 102 Chinesesubjects and 101 Dayaknese subjects, the prevalence of H.pylori infection was40.8%. The prevalence among Chinese is higher than Dayaknese, which is 48.0%and 33.7%, respectively. There is no difference in the epidemiologicalcharacteristics and endoscopic findings in both ethnic groups.Conclusions : The prevalence of H. pylori infection among the Chinese (48.0%)is higher than among Dayaknese (33.7%). There is no difference in theepidemiological characteristics and endoscopic finding among both ethnic groups.]"

"Latar belakang: Dispepsia merupakan gangguan kesehatan yang sering ditemuierologi dan urin (RAPIRUN) dibandingkan dengan UBT sebagai baku emas dalam mengetahui infeksi H. pylori. Penelitian dilakukan pada pasien rawat jalan Puskesmas Kecamatan Koja Kotamadya Jakarta Utara. Yang dinilai adalah sensitivitas, spesivisitas, positive predictive value (PPV), negative predictive value (NPV) tes tersebut. Tujuan: Mengetahui akurasi diagnostik pemeriksaan non-invasif (serologi dan urin) dibandingkan dengan UBT (urea breath test) sebagai baku emas untuk mendeteksi infeksi H. pylori pada pasien dengan sindroma dispepsia. Metode: Penelitian yang digunakan adalah studi potong lintang untuk mengetahui akurasi pemeriksaan non-invasif yaitu s H. pylori menunjukkan hasil positif pada 36,5% subyek, sedangkan pada pemeriksaan serologi (Mataram, Biomedika) didapatkan hasil positif sebanyak 32,4%. Pemeriksaan RAPIRUN (Rapid Urine Test, Otsuka) menunjukkan hasil positif pada 24,3% subyek. Pada serologi didapatkan sensitivitas 74%, spesifitas 91%, PPV 83%, NPV 86%. Sedangkan pada RAPIRUN didapatkan sensitivitas 63%, spesifitas 98%, PPV 94%, NPV 82%. Hasil: Selama kurun waktu April 2015 sampai Juni 2015, 74 subyek, dengan mayoritas perempuan (82,4%), dengan rerata umur 45,05 tahun menjalani pemeriksaan non-invasif. Pemeriksaan UBT sebagai baku emas diagnosis infeksi di pelayanan kesehatan. Infeksi Helicobacter pylori adalah salah satu penyebab dispepsia. Diagnosis infeksi H.pylori dapat dilakukan melalui pemeriksaan invasif dan non invasif. Pemeriksaan non invasif lebih mampu laksana, murah dan memiliki risiko yang lebih sedikit. Simpulan: RAPIRUN lebih unggul dalam hal spesifisitas dibanding serologi.

---

Background: Dyspepsia is the common problem in the population. The main etiology of dyspepsia is Helicobacter pylori infection. The diagnosis of H. pylori infection is based on invasive examination and non-invasive examination. The non-invasive examination could be easier to do and have less risk than invasive examination.

Objective: To evaluate the diagnostic accuracy of the non-invasive test (serology and RAPIRUN) compared to UBT as gold standard examination to detect H. pylori infection in patients with dyspepsia syndrome.

Methods: A cross-sectional study for diagnostic H. pylori by using serology and Rapid Urine test (RAPIRUN) is conducted to evaluate the diagnostic accuracy of non-invasive test compared to UBT as gold standard examination in patients with dyspepsia syndrome. This study was conducted at outpatient Community Health Center in Koja District North Jakarta from middle April 2015 until Middle June 2015. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) were used to evaluate the diagnostic accuracy.

Results: From mid-April 2015 to Mid-June 2015, 74 subjects, with the majority of patients was female (82.4%), and the mean of age was 45.05 years old, had undergone non-invasive test The UBT test as the gold standard examination for H. pylori infection showed positive result in 36.5% patients while the serology test resulting positive in 32.4%. The RAPIRUN test resulting positive in 32.4% patients. The sensitivity of serology test was 74%, specificity 91%, PPV 83%, NPV 86%, meanwhile the RAPIRUN test was resulting as sensitivity 63%, specificity 98%, PPV 94%, NPV 82%.

Conclusion: RAPIRUN has a high diagnostic value for H. pylori in specificity than serology."

"Tujuan Pengobatan untuk eradikasi H pylori dengan kombinasi PPI dan Amoksisilin 1000 mg atau Clarithromycin 500 mg yang diberikan 2 kali sehari selama 2 minggu telah terbukti efektif. Kebanyakan penelitian menyebutkan bahwa pengobatan 7 dan 10 hari sama efektif. Tujuan dari penelitian ini adalah untuk membandingkan terapi eradikasi H pylori selama 5 dan 7 hari. Metode Penelitian ini dilakukan secara prospektif pada 60 pasien yang terinfeksi H pylori, pemeriksaan dilakukan dengan endoskopi dan biopsi saluran cerna bagian atas di 6 (enam) rumah sakit di Indonesia. Diagnosis H pylori ditegakkan berdasarkan pada 2 pemeriksaan rapid urea test (Pronto Dry) dan pemeriksaan histopatologi atau adanya urea breath test (UBT) yang positif. Kemudian secara terbuka pasien akan dibagi menjadi 2 kelompok, kelompok pertama mendapatkan pengobatan oral amoksisilin 1000 mg 2 kali sehari, clarithromycin oral 500 mg 2 kali sehari, rabeprazole 10 mg 2 kali sehari selama 5 hari dan kelompok kedua mendapatkan pengobatan oral amoksisilin 1000 mg 2 kali sehari, clarithromycin oral 500 mg 2 kali sehari, rabeprazole 10 mg 2 kali sehari selama 7 hari. Empat minggu kemudian, pasien dievaluasi dengan UBT untuk memastikan keberadaan H pylori. Hasil Pada penelitian ini didapatkan 60 pasien yang terdiri dari 42 laki-laki dan 18 perempuan dengan rata-rata (usia+SD) 47.63+13.93 tahun, rentang usia 21-74 tahun. Kelompok pengobatan 5 hari terdiri dari 25 (41,7%) pasien dan kelompok pengobatan 7 hari terdiri dari 35 (58.3%) pasien. Setelah kelompok pengobatan 5 hari didapatkan 18 (72%) pasien H Pylori negatif, dan setelah pengobatan 7 hari didapatkan 32 (91.4%) pasien H Pylori negatif. Kegagalan eradikasi ditemukan pada kelompok pengobatan 5 hari sebesar 7 (28%) pasien dan pada kelompok pengobatan 7 hari sebesar 3 (8.6%) (p=0,077). Kesimpulan Penelitian ini menunjukkan bahwa eradikasi H pylori dengan pengobatan tripel dengan rabeprazole pada kelompok 7 hari masih lebih baik daripada kelompok pengobatan 5 hari. Perbedaan yang tidak bermakna secara statistik ini mungkin karena jumlah sampel tidak mencukupi.

---

Aim A combination of PPI and 1000 mg amoxicillin/500 mg clarithromycin twice daily for 2 weeks has been proven effective in the eradication of H. pylori. Most studies suggested that treatment for 7 and 10 days may be equally effective. Few data are available on the effi cacy of 5-day triple therapy. Aim of this study was to compare 5-day and 7-day rabeprazole triple therapy for eradication of H. pylori infection. Methods We prospectively studied 60 consecutive H. pylori-infected patients who came to hospitals in six centres in Indonesia and who underwent upper endoscopy and biopsy. H. pylori infection was confi rmed if two rapid urease tests (Pronto Dry) and histology or urea breath test were positive. Patients were assigned to either an open-labelled 5-day or 7-day course of oral amoxicillin 1000 mg b.i.d., clarithromycin 500 mg b.i.d., and rabeprazole 10 mg b.i.d. (RAC).Four weeks after therapy, all patients had a repeated UBT for evaluation of the presence of H. pylori. Results Of the 60 patients (42 males and 18 females) with mean age (± SD) 47.63 ± 13.93 years, range 21?74 years, 25 patients (41.7%) had 5-day treatment and 35 patients (58.3%) had 7-day treatment. With 5-day treatment, 18 patients (72%) and with 7-day treatment 32 patients (91.4%) became negative for H. pylori infection. The eradication failure was found on 7 patients (28.0%) in 5-day reatment and 3 patients (8.6%) in 7-day treatment. Conclusions The study showed that the eradication of H. pylori infection by triple rabeprazole-based treatment in 7-day is still better than in 5-day."

"Kami melaporkan sebuah kasus seorang laki-laki, 72 tahun, etnis Tionghoa dengan keluhan utama buangair besar berwarna hitam lembek. Pemeriksaan fisik menunjukkan warna pucat pada konjungtiva palpebrayang dikonfirmasi dengan hasil hitung darah lengkap. Pemeriksaan gastroduodenoskopi menemukan adanyaulkus berukuran 3 mm di antrum (Forrest stage III). Infeksi H. pylori dinyatakan positif berdasarkan limametode berbeda (urinary antibody tests, rapid urease test, kultur, histologi dan imunohistokimia). Analisisdengan sequencing berbasis polymerase chain reaction didapatkan bahwa pasien terinfeksi oleh strainberjenis East-Asian-type cagA dan vacA s1m1. Analisis lanjutan dengan menggunakan tujuh housekeepinggen mengkonfirmasikan bahwa strain tersebut tergolong dalam kelompok hspEAsia. Pasien diberikan infusintravena kontinyu pompa proton inhibitor dan standar triple therapy regimens untuk terapi eradikasi H. pylori.We reported a male, 72 yo, Chinese ethnic with chief complaint black mushy defecation. Physical examinationrevealed pale on conjunctival palpebra which confirmed as anemia on complete blood count. Gastroduodenoscopyrevealed a 3 mm ulcer at the antrum (Forrest stage III). H. pylori infection was positive based on five differenttest methods (urinary antibody tests, rapid urease test, culture, histology ad immunohistochemistry). Usedpolymerase chain reaction-based sequencing, we found the patient infected by CagA producing, East-Asian-typecagA and vacA s1m1-strain. Further analysis using 7 housekeeping genes confirmed that the strain categorizedin to hspEAsia group. The patient was given continuous intravenous infusions of proton pump inhibitor andstandard triple therapy regimens eradication of H. pylori."