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"Penelitian keragaman genetik udang mantis di Perairan Pelabuhan ratu dan Cirebon telah dilakukan pada bulan Februari ndash; November 2016. Penelitian ini bertujuan untuk mengetahui keragaman genetik udang mantis di perairan Pelabuhan ratu dan Cirebon. Identifikasi udang mantis menggunakan karakter morfologi dan DNA barcoding dengan menggunakan Cytochrome oksidase sub unit I COI . Analisis karakter morfologi menggunakan software PAST v.3.14 Paleontological Statistics dengan metode cluster. Rekonstruksi pohon filogenetik menggunakan software MEGA 6 dengan metode Neighbour Joining berdasarkan model Tamura-3 paramater dengan bootstrap 1000 kali. Hasil penelitian menunjukkan bahwa stomatopoda yang ditemukan dari lokasi pengambilan sampel terdiri atas Harpiosquilla harpax, Oratosquilla oratoria, Oratosquillina gravieri dan Harpiosquilla annandalei. Rata-rata kelimpahan larva Stomatopoda di perairan Cirebon pada stasiun I, II, III dan IV masing-masing 0,047; 0,018; 0,003 dan 0,003 ind/m3sedangkan larva di perairan Pelabuhan ratu hanya ditemukan di stasiun IV sebanyak 0,003 ind/m3. Hasil dendogram karakter morfometrik terdiri atas tiga kelompok, yaitu kelompok H. harpax Cirebon - H.harpax Pelabuhan ratu , kelompok O. oratoria-H. annandalei, dan kelompok O. gravieri. Kesamaan pada kelompok H. harpax dari Cirebon dan Pelabuhan ratu sebesar 94,5 sedangkan H. annandalei ndash; O.oratoria sebesar 92,5 . Hasil rekonstruksi filogenetik yang dibentuk berdasarkan sekuen yang sudah dicocokkan pada Gene bank yaitu terdiri atas 2 genus yaitu Harpiosquilla dan Oratosquilla.

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The study of genetic diversity mantis shrimp in the Pelabuhan Ratu and Cirebon waters was conducted in February November 2016. This study aimed to determine the genetic diversity of the mantis shrimp in the Pelabuhan Ratu and Cirebon waters. Mantis shrimp was identified using morphological characters and DNA barcoding used Cytochrome Oxidase subunit I COI . Analysis character morphological were done using PAST software v.3.14 Paleontological Statistics cluster method. Reconstruction of the phylogenetic tree used MEGA software 6 with Neighbour Joining method based on the model of Tamura 3 parameters by bootstrapping 1000 times. The results showed that stomatopods found from sampling sites consist of Harpiosquilla harpax, Oratosquilla oratoria, Oratosquillina gravieri and Harpiosquilla annandalei. The average abundance of larvae stomatopoda were found in Cirebon waters at station I, II, III and IV 0,047 0,018 0.003 and 0.003 ind m3, respectively, while in the Pelabuhan ratu water fourth station were found as much as 0,003 ind m3. Dendogram of morphometric character consists of three groups, namely H. harpax Cirebon H. harpax Pelabuhan ratu , O. Oratoria H. annandalei group, and the group O. gravieri. Similarities were found H.harpax group of Cirebon and Pelabuhan Ratu as much as 94.5 while H. annandalei O. oratoria was 92.5 . The results of phylogenetic reconstruction were formed by sequences that have been matched in the Gene bank which consists of two genera, Harpiosquilla and Oratosquilla."

"[ABSTRAKTelah dilakukan penelitian mengenai identifikasi spesies dan distribusilarva udang mantis di Teluk Banten selama bulan Oktober 2013--November 2013.Penelitian bertujuan untuk mengukur efektivitas aplikasi DNA barcoding dalamidentifikasi larva udang mantis dan mempelajari pola distribusinya di TelukBanten. Larva udang mantis sebanyak 138 individu dikoleksi denganmenggunakan jaring larva dengan besar mulut 30x30 cm2 dan besar jaring sebesar500 μm dari 6 stasiun penelitian. Daerah COI sebagai penanda DNA barcodingefektif dapat digunakan untuk identifikasi larva udang mantis dengan variasiintraspesies sekuen COI berkisar antara 0,7--2,4%. Distribusi larva udang mantisberpusat di Stasiun 4 yang ditandai dengan tingginya kelimpahan larva udangmantis pada lokasi tersebut (P<0,005; ANOSIM). Ordinasi NMDS danklusterisasi berdasarkan jarak Bray-Curtis menunjukkan distribusi larva udangmantis dipengaruhi oleh kondisi perairanTeluk Banten. Faktor lingkungan yangmemengaruhi kelimpahan larva udang mantis adalah suhu, salinitas dan kecerahandengan nilai R2 adjusted sebesar 94,5% (P<0,05). Distribusi, kelimpahan, dankomposisi larva udnag mantis di Teluk Banten juga dipengaruhi oleh polaperilaku larva (vertical migration) dan arah arus yang memengaruhi perairanTeluk Banten. Distribusi kelimpahan larva pada lokasi penelitian selama bulanOktober--November 2013 bergerak kearah barat Teluk Banten.

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ABSTRACTPlanktonic larvae of stomatopoda were collected at six stations in BantenBay from October 2013 to November 2013, aimed at assessing effectiveness ofusing COI gene for barcoding stomatopoda larvae and studying its distribution inBanten Bay. A total of 138 stomatopod larvae were obtained by deploying larvaltrap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10minutes just beneath the surface. Five species of stomatopod successfullyidentified using COI gene as barcode marker. Variation of intraspecies for COIgene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution ofstomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance atsix stations in Banten Bay affected by temperature, salinity, and visibility withscore of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity ofstomatopods larvae are affected by vertical migration and current on Teluk Bantenwater.;Planktonic larvae of stomatopoda were collected at six stations in BantenBay from October 2013 to November 2013, aimed at assessing effectiveness ofusing COI gene for barcoding stomatopoda larvae and studying its distribution inBanten Bay. A total of 138 stomatopod larvae were obtained by deploying larvaltrap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10minutes just beneath the surface. Five species of stomatopod successfullyidentified using COI gene as barcode marker. Variation of intraspecies for COIgene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution ofstomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance atsix stations in Banten Bay affected by temperature, salinity, and visibility withscore of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity ofstomatopods larvae are affected by vertical migration and current on Teluk Bantenwater.;Planktonic larvae of stomatopoda were collected at six stations in BantenBay from October 2013 to November 2013, aimed at assessing effectiveness ofusing COI gene for barcoding stomatopoda larvae and studying its distribution inBanten Bay. A total of 138 stomatopod larvae were obtained by deploying larvaltrap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10minutes just beneath the surface. Five species of stomatopod successfullyidentified using COI gene as barcode marker. Variation of intraspecies for COIgene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution ofstomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance atsix stations in Banten Bay affected by temperature, salinity, and visibility withscore of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity ofstomatopods larvae are affected by vertical migration and current on Teluk Bantenwater., Planktonic larvae of stomatopoda were collected at six stations in BantenBay from October 2013 to November 2013, aimed at assessing effectiveness ofusing COI gene for barcoding stomatopoda larvae and studying its distribution inBanten Bay. A total of 138 stomatopod larvae were obtained by deploying larvaltrap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10minutes just beneath the surface. Five species of stomatopod successfullyidentified using COI gene as barcode marker. Variation of intraspecies for COIgene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution ofstomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance atsix stations in Banten Bay affected by temperature, salinity, and visibility withscore of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity ofstomatopods larvae are affected by vertical migration and current on Teluk Bantenwater.]"

"Penelitian mengenai kajian keragaman genetik, aspek pertumbuhan dan reproduksi udang mantis di perairan Teluk Banten Serang Propinsi Banten sebagai upaya konservasi dalam pengelolaan sumber daya udang mantis, khususnya Harpiosquilla raphidea telah dilakukan dari bulan Desember 2011 sampai dengan September 2013. Penelitian bertujuan untuk mengkaji dan mengetahui aspekaspek biologi populasi (keragaman genetik, aspek pertumbuhan dan reproduksi). Penelitian dilakukan mengunakan metode survei dan purposive sampling. Lokasi pengambilan sampel udang mantis Harpiosquilla raphidea di perairan Teluk Banten (Provinsi Banten). Hasil Penelitian menunjukkan bahwa keragaman morfologi udang mantis (H. raphidea) yang terekspresi dari perbedaan karakter ukuran tubuh, dari 22 karakter morfologi 20 karakter morfologi berbeda sangat nyata sedangkan 2 karakter lainnya tidak berbeda nyata yaitu lebar abdomen ruas kelima (ASL) dan ruas keenam (ASN). Analisis sekuens nukleotida daerah COI mtDNA udang mantis H. raphidea diperoleh sekuens udang mantis H. raphidea asal Indonesia yang saat ini belum ada di Gene Bank. Pola pertumbuhan udang mantis relatif sama antara jantan dan betina Allometrik (-). Laju pertumbuhan udang mantis Harpiosquilla raphidea tidak jauh berbeda dengan udang mantis lainnya dari Ordo Stomatopoda. Rentang hidup udang mantis di Teluk Banten relatif lebih panjang (5,6 -- 6,7 tahun), dengan nilai pertumbuhan (K) udang mantis jantan lebih tinggi dibandingkan betina. Faktor kondisi udang betina lebih tinggi dari pada jantan dengan nilai pertumbuhan (L¥) antara jantan dan betina pada kisaran yang sama adalah sebesar 369,08 mm. Tingkat kematangan gonad (TKG) udang mantis H. raphidea terindentifikasi dalam empat tingkat perkembangan. Ukuran matang gonad pertama udang mantis betina 199 mm dan udang mantis jantan adalah 230 mm. Udang mantis betina pada TKG II, III dan IV memiliki fekunditas berkisar 24,600--76,809 dengan rata-rata fekunditas 54,082±13,456 butir, sedangkan diameter telur pada TKG III, 510 ?675 μm dengan rata-rata diameter 574 ±6.98 μm. Pengelolaan sumber daya udang mantis di Teluk Banten diarahkan pada kegiatan perbaikan populasi melalui kegiatan konservasi atau upaya domestikasi. Upaya konservasi polulasi melalui pengendalian penangkapan berdasarkan ukuran tangkapan dengan perbaikan habitat serta upaya domestikasi. Populasi Teluk Banten dapat dijadikan sebagai sumber induk dan dapat disilangkan dengan populasi Jambi sebagai selective breeding.

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This study describes the variation in quantitative characters of both Harpiosquilla raphidea only from different location in Indonesia Research on the study of genetic diversity growth and reproductive aspects of mantis shrimp in the waters of the Banten Bay Banten Province as an initial effort in the mantis shrimp resource management particularly Harpiosquilla raphidea been done.

The study aims to assess and determine aspects of population biology genetic differentiation growth and reproductive biology aspects The study was conducted from December 2011 to September 2013 using the survey method and purposive sampling Sampling sites Harpiosquilla raphidea mantis shrimp in the waters of the Banten Bay Banten Province For comparison samples were taken mantis shrimp in the waters of Kuala Tungkal Jambi waters Gebang Kulon Cirebon West Java and water Kubu Raya Pontianak West Kalimantan especially for the analysis of genetic differentiation Research addressing the diversity of morphology of mantis shrimp H raphidea expressed from differences in body size characters morphometric 20 morphometric characters of 22 different characters are very real and 2 were not significantly different character is the fifth segment of the abdomen width ASL and a sixth segment ASN Value farthest distance Banten Bay population is the population of Edinburgh while the population closest to Cirebon Nucleotide sequence analysis of mtDNA COI region of 4 mantis shrimp population dendrogram obtained with the 3 groups clusters first main cluster consisting population Cirebon Jambi Pontianak second cluster population and the third cluster Banten Bay population Farthest genetic distance values based on mtDNA COI sequences are among the Banten Bay population with a population of Jambi While the population closest to Pontianak Aspects of the growth of mantis shrimp have the same relative weight of the long relationship between male and female is a pattern of allometric growth In general the rate of growth of mantis shrimp Harpiosquilla raphidea almost the same and not much different from other mantis shrimp of the order stomatopods but have a life span that is slightly longer ie 5 6 years to 6 7 years Value growth K is higher than male shrimp shrimp females because the female mantis shrimp growth offset to gonadal development and reproductive organs but from the condition factor obtained higher scores than males meaning that the weight gain is also influenced by the gonads and reproductive organs the female mantis shrimp Value growth L only between males and females in the same range of 369 08 mm Reproductive biology into basic information in an effort to better management and conservation efforts of domestication therefore it is very necessary to search the biological aspects of reproduction So the reproductive biology research can provide data and information on the morphological and histological characteristics of gonadal female mantis shrimp and mantis shrimp male Harpiosquilla rahpidea so levels and gonad maturity index can be determined Gonadal matury level of mantis shrimp H raphidea identified in four stages of development Size mature gonads first female mantis shrimp mantis shrimp 199 mm and males is 230 mm Gonadal matury level of mantis shrimp females in II III and IV have fecundity ranged from 24 600 to 76 809 with an average of 54 082 13 456 grains fecundity whereas the diameter of the eggs at Gonadal matury level of III 510 675 m with an average diameter of 574 6 98 mDescriptive method with univariate and multivariate analyses based on quantitative character ratio was used in this study The study aims to assess the distribution of population structure growth and exploitation rate of mantis shrimp Harpiosquilla raphidea Fabricius 1798 Growth coefficient K H raphidea is 0 16 for males and 0 14 for females and Loo is 369 08 same for males and females The value K mantis shrimp H raphidea is same relatively with some other mantis shrimp species such as Squilla mantis Oratosquilla oratoria and O stephensoni but H raphidea have longer life span wich is 5 5 to 6 7 years Mantis shrimp Harpiosquilla raphidea in an abundant marine crustacean in Indonesia It is common amng the most important predators in many shallow tropical and subtropivcal marine habitats The objective of theis study is to provide information on the histological characteristics of the ovary of female mantis shrimp Harpiosquilla rahpidea and spanwned egg The ovaries and spermatogonia a pronounced macroscopic differentiation in size and color with maturation of the ovary and spermatogonia in four developmental The average size of female 199 mm and males mantis shrimp first maturity is 230 mm Female mantis shrimp has a relatively small fecundity which is ranged from 24 600 to 76 809 eggs and eggs diameter in matury stage III is ranged from 510 to 675 m Mantis shrimp resource management in Banten Bay are focused on the repair and conservation of the population Polulasi conservation improvements with domestication and control efforts based on the size of the catchment arrest Banten Bay population may serve as a source of stem and can be crossed with a population of Pontianak and Jambi Recommendations effort did domestication activities in Banten Bay waters can be done by using floating cages enlargement of selected activities on measures that category have not entered the market or small 12 cm Key words genetic diversity growth and reproductive aspects mantis shrimp Banten Bay"

"ABSTRAKGallus gallus domesticus atau yang dikenal sebagai ayam kampung merupakan hasil domestikasi dari jenis ayam hutan merah Gallus gallus gallus. Kabupaten Bangkalan merupakan salah satu Kabupaten yang terletak di Pulau Madura yang memiliki peternakan ayam ketawa hasil domestifikasi dari Kabupaten Sidrap. Penelitian ini bertujuan untuk mengetahui keragaman ayam ketawa dan solusi pemuliaan ayam ketawa untuk mempertahankan sumber daya hayati, oleh karena itu dilakukan penelitian ayam ketawa berdasarkan analisis bioakustik, morfometrik, dan DNA barcoding. Sampel ayam ketawa yang berasal dari Kecamatan Kamal mempunyai rata-rata durasi kokok terpanjang yaitu 6,00 3,0 detik dan rata-rata jumlah suku kata terbanyak berasal dari ayam ketawa Kecamatan Socah yaitu 7,20 5,80. Hasil analisis morfometrik seluruh populasi ayam ketawa di Kabupaten Bangkalan terdapat delapan karakter morfologi yang berbeda signifikan antara populasi ayam ketawa di Kecamatan Kamal dan Kecamatan Burneh yaitu bobot badan, panjang femur, panjang shank, lingkar shank, panjang sayap, tinggi jengger, panjang jari ketiga, dan panjang tulang dada, sedangkan empat karakter morfologi berbeda signifikan antara populasi ayam ketawa di Kecamatan kamal dan Kecamatan Socah yaitu panjang shank, panjang sayap, panjang jari ketiga, dan panjang tulang dada, serta tiga karakter morfologi berbeda signifikan antar individu di Kecamatan Burneh dan Kecamatan Socah yaitu bobot badan, panjang femur, dan lingkar shank. Adanya perbedaan nilai signifikan karakter morfologi antar populasi disebabkan oleh faktor eksternal. Hasil nilai bootstrap tinggi yaitu 1000, sedangkan pada nilai bootstrap terkecil yaitu 382. Jarak genetik sebagai dasar rekonstruksi pohon filogeni yang dihasilkan dari populasi ayam ketawa di Kabupaten Bangkalan yaitu berkisar antara 0,025--1,872. Analisis molekuler melalui teknik DNA Barcoding dengan Gen Cytochrome Oxidase Sub Unit 1 COI dapat digunakan untuk mengidentifikasi spesies ayam ketawa di Kabupaten Bangkalan G. g. domesticus dan persebarannya.

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ABSTRACTGallus gallus domesticus or known as native chicken became domesticated from wild junglefowls Gallus gallus gallus. Bangkalan District is one of the districts located in Madura Island which has breeding domesticated chicken from Sidrap District. The purpose of this research is to know the diversity of ketawa chicken and ketawa chicken Breeding solution to preserve the biological resources, therefore the research of ketawa chicken ketawa is done based on bioacoustic, morphometric, and DNA barcoding analysis. Ketawa chicken samples from Kamal Subdistrict had the longest duration of crowing length of 6.00 3.0 seconds and the average number of syllables ketawa chicken was mostly from Socah Subdistrict of 7.20 5.80. Result of morphometric analysis of whole population of ketawa chicken in Bangkalan District there are eight morphological characters significantly different between ketawa chicken population in Kamal Subdistrict and Burneh Subdistrict are body weight, femur length, shank length, shank circumference, wing length, height of comb, third finger length, and chest length, where as four morphological characters were significantly different between ketawa chicken population in Kamal Subdistrict and Socah Subdistrict, are shank length, wing length, finger length, and chest length, and three significantly different morphological characters between individuals in Burneh Subdistrict and Socah Subdistrict namely body weight, femur length, and shank circumference. The existence of significant difference of morphological character between population is caused by external factor. The result of high bootstrap value is 1000, while at the smallest bootstrap value is 382. Genetic distance as the basis of phylogenetic tree reconstruction resulting from ketawa chicken population in Bangkalan District is ranged from 0,025 1,872. Molecular analysis through DNA Barcoding technique with Cytochrome Oxidase Sub Unit 1 COI Genes can be used to identify Ketawa chicken species in Bangkalan District G. g. domesticus and their distribution."

"Telah dilakukan penelitian untuk mengetahui keragaman genetik udang mantis di perairan pesisir Kabupaten Tangerang selama bulan Januari ? Desember 2015. Pengambilan sampel larva Stomatopoda dilakukan sebanyak 3 kali yaitu pada bulan Februari, Juli dan Sepember pada 4 stasiun penelitian yang terdapat di antara Tanjung Kait dan Muara Sungai Cisadane. Stomatopoda dewasa juga diambil sebanyak 1 kali pengambilan sebagai data konfirmasi dengan identifikasi secara morfologi. Empat spesies udang mantis dewasa berhasil diidentifikasi dari 37 individu yang didadapat. Keempat jenis tersebut antara lain Erugosquilla woodmasoni, Odontodactylus sp., Harpiosquilla harpax, dan Miyakea nepa. Dua puluh empat larva Stomatopoda berhasil didapatkan menggunakan jaring plankton pada 3 kali pengambilan sampel. Hanya 12 larva yang berhasil diamplifikasi gen Cytochrome oxidase sub unit 1 untuk identifikasi molekular menggunakan mesin PCR SimplyAmp Biosystem. Seluruh gen yang berhasil diamplifikasi dikirim ke Macrogen Korea untuk proses sekuensing.Hasil akhir menunjukkan terdapat 4 grup kekerabatan berdasarkan sekuens yang terdapat pada bank gen yaitu Oratosquilla interrupta, Harpiosquilla harpax, Stomatopoda sp. 1 BTN-2013 yang merupakan hasil sekuens penelitian sebelumnya dari Mariana dkk. (2013), dan Oratosquilla oratoria dengan nilai identitas 85% (NCBI 2015). Sebaran Stomatopoda di pesisir Tangerang cenderung menjauhi bagian muara sungai yang ditunjukkan dengan jumlah individu yang didapat lebih banyak pada stasiun 1 dan 3.

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The research was conducted to know mantis shrimp genetic diversity in Tangerang coastal area for periode of January--Desember 2015. The larva of Stomatopoda was sampled 3 times in February, July and September on 4 sampling stations betwen Tanjung Kait and the estuary of Cisadane river. Mature Stomatopoda was sampled once as confirmation data by morfological identification. Four species of mature Stomatopoda had been identified from 37 individuals, those are Erugosquilla woodmasoni, Odontodactylus sp., Harpiosquilla harpax, and Miyakea nepa. Twenty four larvas were found using plankton net in 3 times of sampling. Only 12 from all larvas have been successfully amplified for the sequence of Cytochrome Oxidase sub unit 1 (CO1) using PCR SimplyAmp Biosystem machine. All amplified gene were sent to macrogen for sequencing procedure.

The result shown 4 group closely related to species Oratosquilla interrupta, Harpiosquilla harpax, Stomatopoda sp. 1 BTN-2013 sequence from early research by Mariana et al. (2013), and Oratosquilla oratoria with identities 85% (NCBI 2015). The Distribution of Stomatopoda in Tangerang coastal area tend to getting far from the estuary which are statiun 1 and 3 showed the larger amount of individu."

"Penelitian mengenai keanekaragaman lobster air tawar di Kabupaten Sorong Selatan, Maybrat, dan Jayawijaya dilakukan pada bulan Agustus 2016. Penelitian bertujuan untuk melihat keanekaragaman lobster air tawar melalui analisis morfometrik dan DNA Barcoding. Hasil penelitian ini menunjukkan bahwa karakteristik A1-D6 dari ke empat lokasi pengambilan sampel terdapat perbedaan yang signifikan p.

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Research on the Research on the diversity of freshwater crayfish in Sorong Selatan Regency, Maybrat, and Jayawijaya conducted in August 2016. The study aims to see the diversity of freshwater crayfish through morphometric analysis and DNA Barcoding. These results indicate that the A1 D6 characteristics of all four sampling sites there are significant differences p "

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Cantigi ungu (Vaccinium varingiifolium) merupakan tumbuhan yang teramati hanya dapat tumbuh di daerah pegunungan dengan ketinggian 1800—3340 mdpl di Indonesia. Cantigi ungu memiliki manfaat sebagai sumber makanan dan memiliki kadar antioksidan yang tinggi. Vaccinium spp. di Amerika telah didomestikasi sehingga memiliki nilai komersial. Proses domestikasi tersebut melibatkan identifikasi morfologi, tetapi identifikasi secara molekuler lebih baik digunakan agar breeding menjadi efisien dan efektif. Identifikasi molekuler dapat menggunakan DNA barcoding dan rekonstruksi filogeni. Consortium for the Barcode of Life (CBOL) telah menetapkan bahwa matK dan rbcL merupakan gen penanda (DNA barcoding) yang ideal dalam identifikasi tumbuhan terestrial. Gen matK dan rbcL merupakan gen yang berada pada kloroplas. Hasil analisis filogenetik Cantigi ungu yang ada hanya menggunakan gen penanda ITS. Tujuan dari penelitian ini adalah menganalisis dan mengonfirmasi kekerabatan sekuens Cantigi ungu yang dikoleksi dari Gunung Gede, Gunung Tangkuban Parahu dan Kawah Putih Ciwidey menggunakan gen penanda matK dan rbcL dengan rekonstruksi pohon filogeni. DNA Cantigi Ungu dari tiga tempat berbeda di Jawa Barat diisolasi menggunakan kit ekstraksi DNA tanaman kemudian dilakukan amplifikasi PCR dan optimasi suhu annealing. Suhu annealing optimal Cantigi ungu pada matK adalah 52°C dan rbcL adalah 55°C. Hasil amplifikasi PCR tersebut kemudian disekuensing, dilakukan contig sekuens, di-trimming dan diunggah pada GenBank. Hasil BLAST sekuens ketiga sampel Cantigi ungu pada kedua gen menunjukkan bahwa ketiga sampel Cantigi ungu merupakan spesies yang sama. Hasil analisis alignment menunjukkan bahwa ketiga sampel Cantigi ungu memiliki indeks similaritas 100% pada kedua gen. Hasil Rekonstruksi pohon filogeni dengan Vaccinium spp. dan out-group menunjukkan bahwa ketiga sampel berada pada cabang yang sama, mengindikasikan bahwa ketiga sampel tersebut berasal dari spesies yang sama, Vaccinium varingiifolium.

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The Purple Cantigi (Vaccinium varingiifolium) is a plant observed to only grow in mountainous areas at altitudes of 1800—3340 meters above sea level in Indonesia. The Purple Cantigi has benefits as a food source and possesses a high level of antioxidants. Vaccinium spp. in America has been domesticated, thus having commercial value. The domestication process involves morphological identification, but molecular identification is preferably used for efficient and effective breeding. Molecular identification can utilize DNA barcoding and phylogenetic reconstruction. The Consortium for the Barcode of Life (CBOL) has established that matK and rbcL are ideal marker genes (DNA barcoding) for identifying terrestrial plants. The matK and rbcL genes are located in the chloroplast. The only available results of the phylogenetic analysis of purple Cantigi use the ITS marker gene. This research aims to analyze and confirm the sequence relationships of the Purple Cantigi collected from Mount Gede, Mount Tangkuban Parahu, and Kawah Putih Ciwidey using the matK and rbcL marker genes with phylogenetic tree reconstruction. DNA of the Purple Cantigi from three different places in West Java was isolated using a plant DNA extraction kit and then subjected to PCR amplification and annealing temperature optimization. The optimal annealing temperature for the Purple Cantigi in matK was 52°C, and in rbcL was 55°C. The PCR amplification results were sequenced; contig sequences were performed, trimmed, and uploaded to GenBank. BLAST results of the sequence of the three Purple Cantigi samples on both genes showed that all three samples are the same species. Alignment analysis showed that all three Purple Cantigi samples have a 100% similarity index on both genes. Phylogenetic tree reconstruction with Vaccinium spp. and out-group showed that all three samples are on the same branch, indicating they belong to the same species, Vaccinium varingiifolium.

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