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Madinna Rahmadewi
Identifikasi kapang dari manuskrip Cina lama yang mengalami deteriorasi asal plot 1 Perpustakaan Pusat UI berdasarkan daerah ITS rDNA = Moulds identification from deteriorated old Chinese manuscripts from plot 1 Central Library UI based on ITS rDNA region
"Penelitian ini bertujuan untuk mengidentifikasi kapang dari dua manuskrip Cina lama yang mengalami deteriorasi asal plot 1 Ruang Naskah PP-UI Depok berdasarkan data sekuens daerah internal transcribed spacers ribosomal DNA ITS rDNA . Pengambilan sampel kapang dari manuskrip dengan metode swab dan isolasi kapang dengan metode culture-dependent. Amplifikasi daerah ITS rDNA dan DNA sequencing menggunakan primer forward ITS5 dan primer reverse ITS4.
Pencarian homologi sekuens daerah ITS rDNA menggunakan program basic local alignment search tool BLAST. Pembuatan pohon filogenetik menggunakan metode Neighbor Joining, model dua parameter Kimura dan bootstrap sebanyak 1.000 kali replikasi. Lima isolat kapang terpilih diperoleh berdasarkan tipe morfologi yang berbeda dengan kapang dari manuskrip Cina lama asal plot 2, 4, 5, dan 6.
Hasil elektroforesis gel produk PCR daerah ITS rDNA menunjukkan lima strain memiliki ukuran fragmen ITS rDNA dengan kisaran 500--700 pb dan DNA sequencing menunjukkan panjang daerah ITS rDNA berkisar 579--610 pb. Lima strain UICC merupakan anggota dari dua kelas Class Eurotiomycetes dan Dothideomycetes , dua ordo Order Eurotiales dan Capnodiales serta tiga famili Family Aspergillaceae, Cladosporiaceae dan Trichocomaceae.
Strain UICC 1099 dan UICC 1102 memiliki homologi 99,4 dan 99,8 dengan type strain Aspergillus pseudodeflectus NRRL 6135T. Strain UICC 1103 memiliki homologi 99,7 dengan type strain Cladosporium colocasiae ATCC 200944 T. Strain UICC 1101 memiliki homologi 99,8 dengan type strain Penicillium coffeae NRRL 35363T. Strain UICC 1100 memiliki homologi 99,4 dengan type strain Penicillium mallochii DAOM 239917T. Lima strain UICC merupakan fungi anamorf dan bersifat xerofilik.
The objective of this study was to identify moulds isolated from two deteriorated old Chinese manuscripts from plot 1 Ruang Naskah Central Library Universitas Indonesia Depok based on sequence data of internal transcribed spacer regions of ribosomal DNA ITS rDNA . Sterile cotton swab was used to obtain samples and culture dependent method was used to isolate moulds. Forward primer ITS5 and reverse primer ITS4 were used to amplify ITS rDNA region and sequencing the DNA.
Basic Local Alignment Search Tool BLAST program was used to determine the sequence homology of ITS rDNA region. A phylogenetic tree was constructed by Neighbor Joining method with Kimura rsquo s two parameter model and bootstrap with 1,000 replicates. Five selected mould isolates were obtained based on the morphological type differences compared to moulds from old Chinese manuscripts from plot 2, 4, 5, and 6.
Gel electrophoresis showed that the fragment lengths of ITS rDNA region from five strains were on the range of 500 700 bp and DNA sequencing showed that the length variations of ITS DNA fragments were 579 to 610 bp. The five UICC strains belonged to two classes Class Eurotiomycetes and Dothideomycetes , two orders Order Eurotiales and Capnodiales and three families Family Aspergillaceae, Cladosporiaceae and Trichocomaceae.
UICC 1099 and UICC 1102 strains showed 99.4 and 99.8 homologies with their type strain Aspergillus pseudodeflectus NRRL 6135T. UICC 1103 strain has 99.7 homology with its type strain Cladosporium clocasiae ATCC 200944T. UICC 1101 strain has 99.8 homology with its type strain Penicillium coffeae NRRL 35363T. UICC 1100 strain has 99.4 homology with its type strain Penicillium mallochii DAOM 239917T. The five UICC strains are anamorphic and xerophilic fungi."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2017
S69876
UI - Skripsi Membership  Universitas Indonesia Library
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Mazytha Kinanti Rachmania
Identifikasi kapang dari manuskrip cina lama yang mengalami deteriorasi asal plot 5 Perpustakaan Pusat UI berdasarkan daerah ITS RDNA = moulds identification from deteriorated old chinese manuscripts from plot 5 central library ui based on ITS region of RDNA
" ABSTRAK
Penelitian ini bertujuan untuk memperoleh identitas spesies kapang dari dua manuskrip Cina lama yang mengalami deteriorasi asal plot 5 Ruang Naskah Perpustakaan Pusat Universitas Indonesia PP-UI , Depok berdasarkan data sekuens daerah internal transcribed spacers ribosomal DNA ITS rDNA . Pengambilan sampel pada manuskrip menggunakan metode swab dengan cotton bud steril. Isolasi kapang menggunakan metode culture-dependent. Polymerase chain reaction PCR dan DNA sequencing menggunakan primer forward ITS5 dan primer reverse ITS4. Pencarian homologi sekuens daerah ITS rDNA menggunakan program basic local alignment search tool BLAST dan pembuatan pohon filogenetik menggunakan metode Neighbor Joining, model dua parameter Kimura, serta bootstrap sebanyak 1.000 kali replikasi. Penentuan spesies terdekat dan posisi taksonomi menggunakan analisis filogenetik dan didukung oleh data morfologi. Isolasi kapang menghasilkan enam isolat kapang terpilih berdasarkan tipe morfologi yang berbeda dengan kapang dari manuskrip Cina lama asal plot 1, 2, 4, dan 6 Ruang Naskah PP-UI, Depok. Berdasarkan elektroforesis gel, panjang fragmen daerah ITS rDNA dari enam isolat kapang bervariasi antara 600--700 pb. Hasil DNA sequencing lengkap menunjukkan panjang daerah ITS rDNA enam isolat berkisar 582--625 pb. Enam strain UICC merupakan anggota dari tiga kelas Dothideomycetes, Eurotiomycetes dan Sordariomycetes , tiga ordo Capnodiales, Eurotiales dan Hypocreales , dan empat famili Cladosporiaceae, Nectriaceae, Ophiocordycipitaceae, dan Pleosporaceae . Strain UICC 1107 memiliki homologi 99,32 dengan type strain Purpureocillium lilacinum sin. Paecilomyces lilacinus ATCC 10114T. Lima strain UICC tidak dapat ditentukan spesiesnya. Strain UICC 1106 adalah Cladosporium sp. dengan homologi 100 terhadap type strain Cladosporium oxysporum CBS 125991T dan Cla. tenuissimum CPC 14235T. Strain UICC 1105 adalah Curvularia sp.1 dengan homologi 93,80 dan strain UICC 1108 adalah Curvularia sp.2 dengan homologi 94,70 terhadap type strain Curvularia carica-papayae CBS 135941T. Strain UICC 1109 adalah Rectifusarium sp. dengan homologi 85,87 terhadap type strain Rectifusarium robinianum CBS 430.91T. Strain UICC 1104 adalah Sarocladium sp. dengan homologi 97,13 terhadap type strain Sarocladium bifurcatum UTHSC 05-3311T. Enam strain UICC merupakan fungi anamorf dan bersifat xerofilik.
ABSTRACT The aim of this study was to determine the species identity of moulds from two deteriorated old Chinese manuscripts from plot 5 Ruang Naskah Central Library Universitas Indonesia, Depok based on internal transcribed spacers region of ribosomal DNA ITS rDNA . Samples from the manuscripts were collected by using swab method with sterile cotton swabs. Mould isolates were obtained by culture dependent method. Polymerase chain reaction PCR and DNA sequencing were performed using forward primer ITS5 and reverse primer ITS4. Homology search of ITS rDNA sequences was carried out using basic local alignment search tool BLAST program and phylogenetic tree construction was performed using Neighbor Joining method, Kimura rsquo s two parameter model, and bootstrap 1,000 replicates. The closest species and taxonomic position were obtained by phylogenetic analysis and supported by morphological data. Six mould isolates were selected based on morphological type differences compared to mould isolates from old Chinese manuscripts from plot 1, 2, 4, and 6 Ruang Naskah Central Library UI, Depok. Based on gel electrophoresis, the lengths of ITS rDNA fragments of six mould isolates varied between 600 700 bp. Full sequence data of ITS rDNA of six isolates showed that the lengths of their ITS rDNA varied between 582 625 bp. Six UICC strains belonged to three classes Dothideomycetes, Eurotiomycetes and Sordariomycetes , three orders Capnodiales, Eurotiales and Hypocreales , and four families Cladosporiaceae, Nectriaceae, Ophiocordycipitaceae, and Pleosporaceae . UICC 1107 strain showed 99.32 homology to the type strain, Purpureocillium lilacinum syn. Paecilomyces lilacinus ATCC 10114T. Five UICC strains were not able to be determined to the species level. UICC 1106 strain was identified as Cladosporium sp., with 100 homology to the type strains, Cladosporium oxysporum CBS 125991T and Cla. tenuissimum CPC 14235T. UICC 1105 strain was identified as Curvularia sp.1, with 93.80 homology and UICC 1108 strain was identified as Curvularia sp.2, with 94.70 homology to the type strain, Curvularia carica papayae CBS 135941T. Strain UICC 1109 was identified as Rectifusarium sp., with 85.87 homology to the type strain, Rectifusarium robinianum CBS 430.91T. Strain UICC 1104 was identified as Sarocladium sp., with 97.13 homology to the type strain, Sarocladium bifurcatum UTHSC 05 3311T. Six UICC strains were anamorphic and xerophilic fungi."
Depok: Universitas Indonesia, 2016
S66193
UI - Skripsi Membership  Universitas Indonesia Library
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Teguh Prasetia Teja
Identifikasi kapang dari manuskrip berbahan kertas eropa berdasarkan analisis sekuens daerah its pada rDNA = Identification of moulds from old european paper manuscripts based on sequence analysis of rDNA its region
"Penelitian ini bertujuan untuk mengidentifikasi 16 strain kapang dari tujuh manuskrip kuno berbahan kertas Eropa dengan analisis sekuens daerah internal transcribed spacers (ITS) pada rDNA dan melakukan deskripsi morfologi terhadap kapang-kapang tersebut. Kapang tersebut berasal dari manuskrip kuno asal Keraton Kasepuhan Cirebon dan Perpustakaan Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia (FIB UI). Amplifikasi daerah ITS rDNA menggunakan forward primer ITS 1 dan reverse primer ITS 4. Deskripsi morfologi dilakukan pada medium Czapek’s Dox Agar (CDA). Panjang fragmen daerah ITS berdasarkan elektroforesis gel dari kapang genus Aspergillus adalah 500--800 pb, Penicillium 500--600 pb, dan mycelia sterilia 550--800 pb. Satu strain kapang merupakan anggota filum Ascomycota, kelas Loculoascomycetes,ordo Dothideales, famili Davidielaceae, dan memiliki homologi sekuens daerah ITS dengan spesies terdekatnya, yaitu Cladosporium cladosporioides (Fresen.).
Sebanyak 13 strain merupakan anggota filum Ascomycota, kelas Plectomycetes,ordo Eurotiales, famili Trichocomaceae, dan memiliki homologi sekuens daerah ITS dengan spesies terdekatnya, yaitu Aspergillus flavus Link. (satu strain),Aspergillus oryzae Cohn. (dua strain), Aspergillus niger (dua strain), Penicillium citrinum Thom (empat strain), Penicillium griseofulvum Dreckx. (satu strain),Penicillium janthinellum Biourd (satu strain), Eurotium amstelodami L. Mangin (satu strain), dan Eurotium rubrum Jos. Konig. (satu strain). Dua strain kapang lainnya, yaitu satu strain Aspergillus sp. dan satu strain Penicillium sp. belum berhasil diidentifikasi hingga tingkat spesies.
The aim of this research was to identify 16 strains of moulds from seven old European paper manuscripts based on sequence analysis of ITS region rDNA and to describe their morphology. The moulds were isolated from old manuscripts from Keraton Kasepuhan Cirebon and the library of Faculty of Humanities University of Indonesia. ITS 1 and ITS 4 primers were used as forward and reverse primers for PCR, respectively. The mould’s morphology was examined on Czapek’s Dox Agar (CDA). The lengths of ITS region of genus Aspergillus based on gel electrophoresis were on the range of 500--800 bp, Penicillium 500--600 bp, and mycelia sterilia 550--800 bp. One strain belongs to phylum Ascomycota, class Loculoascomycetes, order Dothideales, family Davidielaceae,and showed ITS region similarity to Cladosporium cladosporioides (Fresen.).
Thirteen strains belong to phylum Ascomycota, class Plectomycetes, order Eurotiales, family Trichocomaceae, and showed ITS region similarities to Aspergillus flavus Link. (one strain), Aspergillus oryzae Cohn. (two strains),Aspergillus niger (two strains), Penicillium citrinum Thom (four strains),Penicillium griseofulvum Dreckx. (one strain), Penicillium janthinellum Biourd (one strain), Eurotium amstelodami L. Mangin (one strain), and Eurotium rubrum Jos. Konig. (one strain). One strain of Aspergillus sp. and one strain of Penicillium sp. were unable to be identified to species level."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2013
S46010
UI - Skripsi Membership  Universitas Indonesia Library
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Roni Wongso
Identifikasi Kapang dari Manuskrip Kuno Berbahan Daluang Berdasarkan Analisis Sekuens Daerah ITS pada rDNA = Identification of Moulds from Old Manuscripts of Daluang Paper Based on ITS region of rDNA Sequence Analysis
"Penelitian ini bertujuan untuk mengidentifikasi 15 strain kapang dari lima manuskrip kuno berbahan kertas daluang asal perpustakaan Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia (FIB UI) dan melakukan deskripsi morfologi kapang-kapang tersebut. Identifikasi dilakukan berdasarkan analisis sekuens daerah Internal Transcribed Spacers (ITS) rDNA dan pengamatan morfologi kapang dilakukan pada Czapek’s Dox Agar (CDA). Primer forward ITS1 dan primer reverse ITS4 digunakan untuk amplifikasi daerah ITS rDNA.
Hasil elektroforesis produk PCR menunjukkan panjang daerah ITS dari 15 strain kapang tersebut bervariasi antara 500 pb--900 pb. Sebelas strain kapang memiliki homologi ITS rDNA dengan spesies terdekat Aspergillus clavatus Desm. (1 strain), Aspergillus flavus group (1 strain), Aspergillus niger van Tieghem (1 strain), Penicillium citrinum Thom (6 strain), Penicillium janthinellum Biourge (1 strain), dan Penicillium oxalicum Currie & Thom (1 strain) dan termasuk anggota ordo Eurotiales, kelas Plectomycetes, dari filum Ascomycota. Satu strain memiliki homologi ITS rDNA dengan spesies terdekat Pseudocercospora sp. (1 strain) dan termasuk anggota ordo Capnodiales, kelas Dotthideomycetes, dari filum Ascomycota. Tiga strain kapang (Penicillium sp. FIB.PRI.6.1, Fraseriella sp. FIB.PRI.6.2, dan mycelia sterilia FIB.PRII.3) belum berhasil diidentifikasi hingga tingkat spesies.
The aims of this research were to identify 15 mould strains from five old manuscripts of daluang paper from the library of Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia (FIB UI) and to describe their morphology. Identification was carried out based on analysis of Internal Transcribed Spacers (ITS) region of rDNA sequence. Observation of the mould’s morphology was carried out on Czapek’s Dox Agar (CDA). Forward primer ITS1 and reverse primer ITS4 were used to amplify the ITS region of rDNA. Gel electrophoresis results showed that the lengths of ITS region from 15 mould strains were on the range of 500 bp--900 bp.
Eleven strains showed ITS rDNA sequence similarities to Aspergillus clavatus Desm. (1 strain), Aspergillus flavus group (1 strain), Aspergillus niger van Tieghem (1 strain), Penicillium citrinum Thom (6 strains), Penicillium janthinellum Biourge (1 strain), Penicillium oxalicum Currie & Thom (1 strain). The strains belong to order Eurotiales, Class Plectomycetes, phylum Ascomycota. One strain showed ITS rDNA sequence similarity to Pseudocercospora sp. (1 strain). The strain belongs to order Capnodiales, class Dotthideomycetes, phylum Ascomycota. Three strains (Penicillium sp. FIB.PRI.6.1, Fraseriella sp. FIB.PRI.6.2, and mycelia sterilia FIB.PRII.3) were unable to be identified to species level."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2013
S46009
UI - Skripsi Membership  Universitas Indonesia Library
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Trifena Krista Mustikaning Sekar
Pengujian Kemampuan Kapang dari Manuskrip Cina Lama Koleksi Perpustakaan Universitas Indonesia Menggunakan Kertas Cina sebagai Substrat = Evaluation of Moulds from Old Chinese Manuscript-Collection of Universitas Indonesia Library to Utilize Chinese Paper as a Substrate
"Fungi pada manuskrip lama dapat menyebabkan deteriorasi. Tujuan penelitian untuk memperoleh identitas genus kapang dari manuskrip cina lama koleksi Perpustakaan Universitas Indonesia berdasarkan karakter morfologi dan kemampuan kapang menggunakan kertas Cina sebagai substrat. Kapang berasal dari manuskrip cina lama yang mengalami deteriorasi asal plot 2, 4, dan 6 Ruang Naskah Perpustakaan UI, Depok. Empat isolat kapang pada Potato Dextrose Agar (PDA) dan Malt Extract Agar (MEA) dikarakterisasi berdasarkan morfologi sampai genus. Biakan untuk suspensi sel dipersiapkan dengan teknik gores pada PDA miring, inkubasi di suhu 26,5 oC, selama 7 hari. Tiga mL suspensi sel isolat kapang, dan A. versicolor UICC 1037 masing-masing diinokulasikan pada 27 mL Czapek Dox Broth (CDB) tanpa sumber karbon dengan penambahan kertas cina (diameter 5,2 cm) sebagai substrat, dan pada CDB tanpa kertas cina sebagai kontrol. Inkubasi pada suhu 28 oC selama 30 hari. Hasil menunjukkan tiga isolat termasuk Aspergillus P. Micheli ex Haller dan satu isolat termasuk Penicillium Link. Semua isolat dapat menggunakan kertas cina sebagai substrat mengandung sumber karbon dan nutrien untuk pertumbuhan berdasarkan adanya hifa dan sporulasi, penurunan pH medium (dari 8 menjadi 6), perubahan kondisi kertas (bentuk menjadi terlipat atau remuk, struktur menjadi rapuh, warna menjadi kekuningan atau kecokelatan), dan persentase pengurangan berat kering kertas (1,57—6,66%).
Fungi on old manuscripts cause deterioration. The aims of this study were to characterize moulds from old Chinese manuscripts, collection of Universitas Indonesia, by morphology to the genus and evaluate the mould ability to utilize chinese paper as a substrate. The moulds were obtained from deteriorated old Chinese manuscripts from plot 2, 4, and 6 Ruang Naskah UI Library. Four mould isolates on Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA) were characterized by morphology to the genus. Cultures were inoculated on PDA slants by streak method, incubated at 26,5 oC for seven days, and used for cell suspensions in 5 mL sterile water. Three mL cell suspensions of four isolates and A. versicolor UICC 1037, each, were inoculated to 27 mL Czapek Dox Broth (CDB) without a carbon source with the addition of chinese paper (5.2 cm in diameter) as a substrate, incubated at 28 oC for 30 days. Three isolates were characterized as Aspergillus P. Micheli ex Haller and one isolate was characterized as Penicillium Link. All isolates were able to utilize chinese paper as a substrate containing a carbon source dan nutrient for growth, based on the presence of hyphae and sporulation, a decrease in medium pH (from 8 to 6), changes of paper conditions (paper shape became folded or mashed, paper structure became fragile, paper color changed to yellowish or brown), and percentage of loss of paper dry weight (1.57—6.66%)
"
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2021
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
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Annisa Dwi Aprilina
Pengujian Kemampuan Kapang dari Manuskrip Dluwang Lama Asal Cirebon Menggunakan Kertas Merang sebagai Substrat = Evaluation of Moulds From Old Dluwang Manuscripts From Cirebon to Utilize Rice Straw Paper as a Substrate
"Kapang menyebabkan deteriorasi pada manuskrip dluwang lama di Indonesia. Penelitian bertujuan untuk mengetahui kemampuan kapang asal manuskrip dluwang lama dari Cirebon (Keraton Kasepuhan dan Mertasinga) dalam menggunakan kertas merang sebagai substrat. Deskripsi empat strain kapang yang telah diidentifikasi secara molekuler pada penelitian sebelumnya, dikonfirmasi berdasarkan karakterisasi morfologi pada Potato Dextrose Agar (PDA) dan Malt Extract Agar (MEA). Biakan kapang dalam PDA miring di suhu 26,5 oC, umur 7 hari digunakan untuk pembuatan suspensi sel dalam akuades steril 5 ml. Tiga ml suspensi sel diinokulasikan ke dalam 27 ml Czapek Dox Broth (CDB) tanpa sumber karbon dengan penambahan kertas merang (diameter 5,2 cm) sebagai substrat, dan pada 27 ml CDB tanpa kertas merang sebagai kontrol, inkubasi di suhu ruang (28 oC), selama 30 hari. Hasil karakterisasi morfologi mengkonfirmasi empat strain kapang adalah Penicillium rubens Biourge UICC 1062, Aspergillus jensenii Jurjević, S.W. Peterson & B.W. Horn UICC 1069, Cladosporium colocasiae Sawada UICC 1071, dan Eurotium rubrum Jos. König, E. Spieckermann & W. Bremer UICC 1006. Semua kapang menggunakan kertas merang sebagai substrat untuk sumber karbon dan nutrien berdasarkan adanya pertumbuhan (hifa dan sporulasi), perubahan kondisi kertas (kertas menjadi robek, rapuh, adanya titik sporulasi berwarna kehijauan dan cokelat kehitaman), dan persentase pengurangan berat kering kertas (3,44--15,92%).
Moulds causes deterioration on old dluwang manuscripts in Indonesia. This study aims to determine the ability of moulds from the old dluwang manuscripts from Cirebon (Keraton Kasepuhan and Mertasinga) in using rice straw paper as a substrate. Four mould strains which were identified by molecular method in previous study, were described to confirm their species identities based on morphology characterisation on Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA). Seven days-old mould cultures in PDA slants, at 26.5 oC, were used for cell suspensions in 5 ml sterile water. Three ml cell suspensions were inoculated into 27 ml Czapek Dox Broth (CDB) without a carbon source with the addition of rice straw paper (5.2 cm in diameter) as a substrate, and into 27 ml CDB without a carbon source and rice straw paper as a control, incubated at room temperature (28 oC), for 30 days. The results showed that description of four mould strains was confirmed as Penicillium rubens Biourge UICC 1062, Aspergillus jensenii Jurjević, S.W. Peterson & B.W. Horn UICC 1069, Cladosporium colocasiae Sawada UICC 1071, and Eurotium rubrum Jos. König, E. Spieckermann & W. Bremer UICC 1006. All mould strains were able to use rice straw paper as a substrate to obtain carbon source and nutrient for growth based on the presence of hyphae and sporulation, changes in paper conditions (tear, fragile, sporulation spots in light green and brownish-black) and percentage of paper dry weight loss (3.44--15.92%).
"
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2021
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
cover
I Gusti Ngurah Sila Adiharta Jaksa
Pengujian Kemampuan Kapang dari Manuskrip Eropa Lama Asal Keraton Kasepuhan Cirebon Menggunakan Kertas Whatman sebagai Substrat = Investigation of Fungi from Old European Manuscripts from Keraton Kasepuhan Cirebon to Utilize Whatman Paper as a Substrate
"Kapang memiliki kemampuan untuk menggunakan kertas sebagai substrat dan menyebabkan deteriorasi pada kertas. Penelitian ini bertujuan untuk mengkarakterisasi isolat hingga tingkat genus secara morfologi dan mengetahui kemampuan kapang dari manuskrip Eropa lama asal Keraton Kasepuhan Cirebon menggunakan kertas Whatman sebagai substrat. Empat isolat kapang pada Potato Dextrose Agar (PDA) dan Malt Extract Agar (MEA) dikarakterisasi secara morfologi, di suhu 26,5°C, umur 7 hari. Isolat-isolat kapang tersebut diinokulasikan pada PDA miring dengan teknik gores, diinkubasi di suhu 26,5°C selama 7 hari. Biakan disuspensikan dalam akuades steril 5 ml, kemudian 3 ml suspensi sel empat isolat kapang dan Aspergillus versicolor (Vuill.) Tiraboschi UICC 1037 masing-masing diinokulasikan pada 27 ml Czapek Dox Broth (CDB) tanpa sumber karbon dengan penambahan kertas Whatman (diameter 6,4 cm), dan pada CDB tanpa kertas Whatman sebagai kontrol. Inkubasi pada suhu 26,5°C selama 30 hari. Hasil karakterisasi menunjukkan dua isolat termasuk Aspergillus Micheli, satu isolat termasuk Penicillium Link dan satu isolat termasuk Cladosporium Link. Hasil pengujian menunjukkan semua isolat memiliki kemampuan untuk menggunakan kertas Whatman sebagai substrat dan nutrien yang ditunjukkan dengan adanya hifa dan sporulasi, penurunan pH medium (dari 8 menjadi 6), perubahan bentuk dan struktur kertas (bentuk kertas tidak utuh dan tidak bulat, sobek, terlipat, ukuran kertas mengecil), perubahan warna kertas menjadi kuning-kecokelatan, dan pengurangan berat kering kertas dengan kisaran persentase 1,828—75,025%.
Fungi have the ability to utilize paper as a substrate and cause manuscript deterioration. This research aims were to characterize moulds to the genus level by morphology and to investigate moulds from old European manuscripts from Keraton Kasepuhan Cirebon to utilize Whatman paper as a substrate. Four mould isolates on Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA) were characterized by morphology at 26.5°C, for 7 days. The mould isolates were inoculated on PDA slants using streak technique, and incubated at 26.5°C, for 7 days. Cell suspensions in five ml of sterile water were prepared. Three ml cell suspension of each mould isolate and Aspergillus versicolor (Vuill.) Tiraboschi UICC 1037, were inoculated into 27 ml of Czapek Dox Broth (CDB) without a carbon source with addition of Whatman paper (6.4 cm in diameter) as a sole carbon source, and into 27 ml CDB without Whatman paper as control. The cultures were incubated at 26.5°C and observed for 30 days. The result showed two isolates belonged to Aspergillus Micheli, one isolate belonged to Penicillium Link, and one isolate belonged to Cladosporium Link. All isolates were able to utilize Whatman paper as a substrate and nutrient by the presence of hyphae and sporulation, a decrease in medium pH (from 8 to 6), changes in paper shape and structure (paper shape was not completely intact and round, folded, smaller in size), changes of paper colour to brownish-yellow, and the loss in dry weight of Whatman paper in the range of 1.828—75.025%."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2021
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
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Kenardo
Identifikasi kapang endofit ES5, ES6, ES7, dan ES8 dari broussonetia papyrifera vent. dan pengujian aktivitas antimikroba terhadap mikroba uji = Identification of ES5, ES6, ES7, dan ES8 fungal endophytes from broussonetia papyrifera vent. and their antimicrobial test
"Penelitian bertujuan mengisolasi dan mengidentifikasi kapang endofit dari Broussonetia papyrifera, serta mengetahui aktivitas antimikroba kapang endofit terhadap Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633, dan Candida albicans UICC Y-29. Hasil identifikasi konvensional berdasarkan karakter morfologi menunjukkan kapang-kapang endofit terdiri dari Aspergillus flavus ES6, Aspergillus sparsus ES5, Penicillium chrysogenum ES8, dan Mycelia sterilia ES7. Pengujian dengan blok agar memperlihatkan kapang A. flavus ES6 memiliki aktivitas antimikroba terhadap C. albicans dan kapang P. chrysogenum ES8 memiliki aktivitas antimikroba terhadap B. subtilis, sedangkan kapang A. sparsus ES5 dan mycelia sterilia ES7 tidak memperlihatkan aktivitas antimikroba.
This research was to isolate endophytic fungi from Broussonetia papyrifera, to identify the isolates, and to investigate their antimicrobial activity against Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633, and Candida albicans UICC Y-29. Endophytic fungi were identified by conventional method and they were Aspergillus flavus ES6, Aspergillus sparsus ES5, Penicillium chrysogenum ES8, and Mycelia sterilia ES7. Agar block test results of A. flavus ES6 showed antimicrobial activity against C. albicans and P. chrysogenum ES8 against B. subtilis. Aspergillus sparsus ES5 and Mycelia sterilia ES7 showed no antimicrobial activity."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2011
S192
UI - Skripsi Open  Universitas Indonesia Library
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Yura Vebliza
Re-identifikasi lima strain rhizopus arrhizus fischer koleksi UICC berdasarkan data sequence daerah internal transcribed spacers ribosomal dna = Re identification of five strains of rhizopus arrhizus fischer from UICC collection based on internal transcribed spacers region of ribosomal dna sequemce data
"Universitas Indonesia Culture collection (UICC) memiliki koleksi strain-strain Rhizopus arrhizus Fischer yang diisolaasi dari tempe dan telah diidentifikasi berdasarkan karakter morfologi dan fisiologi. Penelitian bertujuan memperoleh identitas yang akurat pada tingkat spesies dari lima strain R. arrhizus (UICC 26, UICC 36, UICC 39, UICC 55, dan UICC 121) berdasarkan data sequence daerah internal transcribed spacers ribosomal DNA dan analisis filogenetik. Amplifikasi dan sequencing daerah ITS rDNA dilakukan menggunakan primer forward ITS5 dan primer reverse ITS4. Pencarian homologi sequence dilakukan dengan program BLAST. Sequence alignment dilakukan menggunakan program Clustal X. Konstruksi pohon filogenetik dilakukan menggunakan metode neighbor joining dengan model dua parameter Kimura dan nilai bootstrap 1000 pengulangan. Karakterisasi morfologi dan fisiologi (pengujian pertumbuhan pada variasi suhu) dilakukan untuk melengkapi deskripsi strain. Panjang fragmen daerah ITS rDNA kelima strain R. arrhizus sekitar 600--700 pb.
Hasil BLAST menunjukkan kelima strain UICC memiliki homologi dengan type strain R. oryzae CBS 112.07T pada kisaran 98,9--99,8%. Pohon filogenetik menunjukkan strain UICC 36 dan strain UICC 55 berada dalam satu grup yang monofiletik dengan type strain R. oryzae CBS 112.07T dan neo type R. arrhizus NRRL 1469NT. Saat ini R. arrhizus merupakan sinonim dari R. oryzae, sehingga kedua strain tersebut diidentifikasi sebagai R. oryzae. Strain UICC 26, UICC 39, dan UICC 121 berada dalam satu grup yang monofiletik dengan type strain R. delemar CBS 120.12T, sehingga ketiga strain diidentifikasi sebagai R. delemar. Rhizopus oryzae dan R. delemar merupakan dua spesies yang berkerabat sangat dekat, sehingga secara morfologi tidak dapat dibedakan. Re-identifikasi lima strain R. arrhizus UICC secara molekuler menghasilkan identitas spesies yang berbeda menjadi R. oryzae dan R. delemar, namun karakter morfologi dan fisiologi lima strain tersebut menunjukkan karakter sebagai R. oryzae.
Universitas Indonesia Culture Collection (UICC) has collection of Rhizopus arrhizus Fischer strains, which were isolated from tempeh and were identified based on morphological and physiological characters. The aim of this study was to obtain accurate identification at species level of five strains of R. arrhizus (UICC 26, UICC 36, UICC 39, UICC 55, and UICC 121) based on internal transcribed spacers (ITS) region of ribosomal DNA (rDNA) sequence data and phylogenetic analysis. Amplification and sequencing of ITS region of rDNA were performed using forward primer ITS5 and reverse primer ITS4. Sequence homology search was performed using BLAST. Sequence alignment was carried out using Clustal X. Construction of phylogenetic tree was performed using neighbor joining method, Kimura?s two parameter model and bootstrap values of 1000 iterations. Characterization of morphological features and growth at variation of temperature were carried out to support the description of the strains. The fragment length of ITS region rDNA from five strains of R. arrhizus was about 600--700 bp.
Results of BLAST homology search of the strains showed 98.9--99.8% similarities to the type strain R. oryzae CBS 112.07T. Phylogenetic tree showed that UICC 36 and UICC 55 were clustered together in a monophyletic group with the type strain R. oryzae CBS 112.07T and neo type strain R. arrhizus NRRL 1469NT. Currently, R. arrhizus is a synonym of R. oryzae, therefore both strains were identified as R. oryzae. Strains UICC 26, UICC 39, and UICC 121 were clustered together in a monophyletic group with the type strain R. delemar CBS 120.12T, therefore they were identified as R. delemar. Rhizopus oryzae and R. delemar are very closely related species and morphologically similar. Re-identification of five strains R. arrhizus UICC based on molecular has difference of species identity as R. oryzae and R. delemar, but the five strains show similar morphological and physiological characters as R. oryzae."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2016
S64952
UI - Skripsi Membership  Universitas Indonesia Library
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Piping through the manuscripts collection
Artikel Jurnal  Universitas Indonesia Library
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