Hasil Pencarian  ::  Simpan CSV :: Kembali

Hasil Pencarian

Ditemukan 71576 dokumen yang sesuai dengan query
cover
Mazytha Kinanti Rachmania
Identifikasi kapang dari manuskrip cina lama yang mengalami deteriorasi asal plot 5 Perpustakaan Pusat UI berdasarkan daerah ITS RDNA = moulds identification from deteriorated old chinese manuscripts from plot 5 central library ui based on ITS region of RDNA
" ABSTRAK
Penelitian ini bertujuan untuk memperoleh identitas spesies kapang dari dua manuskrip Cina lama yang mengalami deteriorasi asal plot 5 Ruang Naskah Perpustakaan Pusat Universitas Indonesia PP-UI , Depok berdasarkan data sekuens daerah internal transcribed spacers ribosomal DNA ITS rDNA . Pengambilan sampel pada manuskrip menggunakan metode swab dengan cotton bud steril. Isolasi kapang menggunakan metode culture-dependent. Polymerase chain reaction PCR dan DNA sequencing menggunakan primer forward ITS5 dan primer reverse ITS4. Pencarian homologi sekuens daerah ITS rDNA menggunakan program basic local alignment search tool BLAST dan pembuatan pohon filogenetik menggunakan metode Neighbor Joining, model dua parameter Kimura, serta bootstrap sebanyak 1.000 kali replikasi. Penentuan spesies terdekat dan posisi taksonomi menggunakan analisis filogenetik dan didukung oleh data morfologi. Isolasi kapang menghasilkan enam isolat kapang terpilih berdasarkan tipe morfologi yang berbeda dengan kapang dari manuskrip Cina lama asal plot 1, 2, 4, dan 6 Ruang Naskah PP-UI, Depok. Berdasarkan elektroforesis gel, panjang fragmen daerah ITS rDNA dari enam isolat kapang bervariasi antara 600--700 pb. Hasil DNA sequencing lengkap menunjukkan panjang daerah ITS rDNA enam isolat berkisar 582--625 pb. Enam strain UICC merupakan anggota dari tiga kelas Dothideomycetes, Eurotiomycetes dan Sordariomycetes , tiga ordo Capnodiales, Eurotiales dan Hypocreales , dan empat famili Cladosporiaceae, Nectriaceae, Ophiocordycipitaceae, dan Pleosporaceae . Strain UICC 1107 memiliki homologi 99,32 dengan type strain Purpureocillium lilacinum sin. Paecilomyces lilacinus ATCC 10114T. Lima strain UICC tidak dapat ditentukan spesiesnya. Strain UICC 1106 adalah Cladosporium sp. dengan homologi 100 terhadap type strain Cladosporium oxysporum CBS 125991T dan Cla. tenuissimum CPC 14235T. Strain UICC 1105 adalah Curvularia sp.1 dengan homologi 93,80 dan strain UICC 1108 adalah Curvularia sp.2 dengan homologi 94,70 terhadap type strain Curvularia carica-papayae CBS 135941T. Strain UICC 1109 adalah Rectifusarium sp. dengan homologi 85,87 terhadap type strain Rectifusarium robinianum CBS 430.91T. Strain UICC 1104 adalah Sarocladium sp. dengan homologi 97,13 terhadap type strain Sarocladium bifurcatum UTHSC 05-3311T. Enam strain UICC merupakan fungi anamorf dan bersifat xerofilik.
ABSTRACT The aim of this study was to determine the species identity of moulds from two deteriorated old Chinese manuscripts from plot 5 Ruang Naskah Central Library Universitas Indonesia, Depok based on internal transcribed spacers region of ribosomal DNA ITS rDNA . Samples from the manuscripts were collected by using swab method with sterile cotton swabs. Mould isolates were obtained by culture dependent method. Polymerase chain reaction PCR and DNA sequencing were performed using forward primer ITS5 and reverse primer ITS4. Homology search of ITS rDNA sequences was carried out using basic local alignment search tool BLAST program and phylogenetic tree construction was performed using Neighbor Joining method, Kimura rsquo s two parameter model, and bootstrap 1,000 replicates. The closest species and taxonomic position were obtained by phylogenetic analysis and supported by morphological data. Six mould isolates were selected based on morphological type differences compared to mould isolates from old Chinese manuscripts from plot 1, 2, 4, and 6 Ruang Naskah Central Library UI, Depok. Based on gel electrophoresis, the lengths of ITS rDNA fragments of six mould isolates varied between 600 700 bp. Full sequence data of ITS rDNA of six isolates showed that the lengths of their ITS rDNA varied between 582 625 bp. Six UICC strains belonged to three classes Dothideomycetes, Eurotiomycetes and Sordariomycetes , three orders Capnodiales, Eurotiales and Hypocreales , and four families Cladosporiaceae, Nectriaceae, Ophiocordycipitaceae, and Pleosporaceae . UICC 1107 strain showed 99.32 homology to the type strain, Purpureocillium lilacinum syn. Paecilomyces lilacinus ATCC 10114T. Five UICC strains were not able to be determined to the species level. UICC 1106 strain was identified as Cladosporium sp., with 100 homology to the type strains, Cladosporium oxysporum CBS 125991T and Cla. tenuissimum CPC 14235T. UICC 1105 strain was identified as Curvularia sp.1, with 93.80 homology and UICC 1108 strain was identified as Curvularia sp.2, with 94.70 homology to the type strain, Curvularia carica papayae CBS 135941T. Strain UICC 1109 was identified as Rectifusarium sp., with 85.87 homology to the type strain, Rectifusarium robinianum CBS 430.91T. Strain UICC 1104 was identified as Sarocladium sp., with 97.13 homology to the type strain, Sarocladium bifurcatum UTHSC 05 3311T. Six UICC strains were anamorphic and xerophilic fungi."
Depok: Universitas Indonesia, 2016
S66193
UI - Skripsi Membership  Universitas Indonesia Library
cover
Madinna Rahmadewi
Identifikasi kapang dari manuskrip Cina lama yang mengalami deteriorasi asal plot 1 Perpustakaan Pusat UI berdasarkan daerah ITS rDNA = Moulds identification from deteriorated old Chinese manuscripts from plot 1 Central Library UI based on ITS rDNA region
"Penelitian ini bertujuan untuk mengidentifikasi kapang dari dua manuskrip Cina lama yang mengalami deteriorasi asal plot 1 Ruang Naskah PP-UI Depok berdasarkan data sekuens daerah internal transcribed spacers ribosomal DNA ITS rDNA . Pengambilan sampel kapang dari manuskrip dengan metode swab dan isolasi kapang dengan metode culture-dependent. Amplifikasi daerah ITS rDNA dan DNA sequencing menggunakan primer forward ITS5 dan primer reverse ITS4.
Pencarian homologi sekuens daerah ITS rDNA menggunakan program basic local alignment search tool BLAST. Pembuatan pohon filogenetik menggunakan metode Neighbor Joining, model dua parameter Kimura dan bootstrap sebanyak 1.000 kali replikasi. Lima isolat kapang terpilih diperoleh berdasarkan tipe morfologi yang berbeda dengan kapang dari manuskrip Cina lama asal plot 2, 4, 5, dan 6.
Hasil elektroforesis gel produk PCR daerah ITS rDNA menunjukkan lima strain memiliki ukuran fragmen ITS rDNA dengan kisaran 500--700 pb dan DNA sequencing menunjukkan panjang daerah ITS rDNA berkisar 579--610 pb. Lima strain UICC merupakan anggota dari dua kelas Class Eurotiomycetes dan Dothideomycetes , dua ordo Order Eurotiales dan Capnodiales serta tiga famili Family Aspergillaceae, Cladosporiaceae dan Trichocomaceae.
Strain UICC 1099 dan UICC 1102 memiliki homologi 99,4 dan 99,8 dengan type strain Aspergillus pseudodeflectus NRRL 6135T. Strain UICC 1103 memiliki homologi 99,7 dengan type strain Cladosporium colocasiae ATCC 200944 T. Strain UICC 1101 memiliki homologi 99,8 dengan type strain Penicillium coffeae NRRL 35363T. Strain UICC 1100 memiliki homologi 99,4 dengan type strain Penicillium mallochii DAOM 239917T. Lima strain UICC merupakan fungi anamorf dan bersifat xerofilik.
The objective of this study was to identify moulds isolated from two deteriorated old Chinese manuscripts from plot 1 Ruang Naskah Central Library Universitas Indonesia Depok based on sequence data of internal transcribed spacer regions of ribosomal DNA ITS rDNA . Sterile cotton swab was used to obtain samples and culture dependent method was used to isolate moulds. Forward primer ITS5 and reverse primer ITS4 were used to amplify ITS rDNA region and sequencing the DNA.
Basic Local Alignment Search Tool BLAST program was used to determine the sequence homology of ITS rDNA region. A phylogenetic tree was constructed by Neighbor Joining method with Kimura rsquo s two parameter model and bootstrap with 1,000 replicates. Five selected mould isolates were obtained based on the morphological type differences compared to moulds from old Chinese manuscripts from plot 2, 4, 5, and 6.
Gel electrophoresis showed that the fragment lengths of ITS rDNA region from five strains were on the range of 500 700 bp and DNA sequencing showed that the length variations of ITS DNA fragments were 579 to 610 bp. The five UICC strains belonged to two classes Class Eurotiomycetes and Dothideomycetes , two orders Order Eurotiales and Capnodiales and three families Family Aspergillaceae, Cladosporiaceae and Trichocomaceae.
UICC 1099 and UICC 1102 strains showed 99.4 and 99.8 homologies with their type strain Aspergillus pseudodeflectus NRRL 6135T. UICC 1103 strain has 99.7 homology with its type strain Cladosporium clocasiae ATCC 200944T. UICC 1101 strain has 99.8 homology with its type strain Penicillium coffeae NRRL 35363T. UICC 1100 strain has 99.4 homology with its type strain Penicillium mallochii DAOM 239917T. The five UICC strains are anamorphic and xerophilic fungi."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2017
S69876
UI - Skripsi Membership  Universitas Indonesia Library
cover
Roni Wongso
Identifikasi Kapang dari Manuskrip Kuno Berbahan Daluang Berdasarkan Analisis Sekuens Daerah ITS pada rDNA = Identification of Moulds from Old Manuscripts of Daluang Paper Based on ITS region of rDNA Sequence Analysis
"Penelitian ini bertujuan untuk mengidentifikasi 15 strain kapang dari lima manuskrip kuno berbahan kertas daluang asal perpustakaan Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia (FIB UI) dan melakukan deskripsi morfologi kapang-kapang tersebut. Identifikasi dilakukan berdasarkan analisis sekuens daerah Internal Transcribed Spacers (ITS) rDNA dan pengamatan morfologi kapang dilakukan pada Czapek’s Dox Agar (CDA). Primer forward ITS1 dan primer reverse ITS4 digunakan untuk amplifikasi daerah ITS rDNA.
Hasil elektroforesis produk PCR menunjukkan panjang daerah ITS dari 15 strain kapang tersebut bervariasi antara 500 pb--900 pb. Sebelas strain kapang memiliki homologi ITS rDNA dengan spesies terdekat Aspergillus clavatus Desm. (1 strain), Aspergillus flavus group (1 strain), Aspergillus niger van Tieghem (1 strain), Penicillium citrinum Thom (6 strain), Penicillium janthinellum Biourge (1 strain), dan Penicillium oxalicum Currie & Thom (1 strain) dan termasuk anggota ordo Eurotiales, kelas Plectomycetes, dari filum Ascomycota. Satu strain memiliki homologi ITS rDNA dengan spesies terdekat Pseudocercospora sp. (1 strain) dan termasuk anggota ordo Capnodiales, kelas Dotthideomycetes, dari filum Ascomycota. Tiga strain kapang (Penicillium sp. FIB.PRI.6.1, Fraseriella sp. FIB.PRI.6.2, dan mycelia sterilia FIB.PRII.3) belum berhasil diidentifikasi hingga tingkat spesies.
The aims of this research were to identify 15 mould strains from five old manuscripts of daluang paper from the library of Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia (FIB UI) and to describe their morphology. Identification was carried out based on analysis of Internal Transcribed Spacers (ITS) region of rDNA sequence. Observation of the mould’s morphology was carried out on Czapek’s Dox Agar (CDA). Forward primer ITS1 and reverse primer ITS4 were used to amplify the ITS region of rDNA. Gel electrophoresis results showed that the lengths of ITS region from 15 mould strains were on the range of 500 bp--900 bp.
Eleven strains showed ITS rDNA sequence similarities to Aspergillus clavatus Desm. (1 strain), Aspergillus flavus group (1 strain), Aspergillus niger van Tieghem (1 strain), Penicillium citrinum Thom (6 strains), Penicillium janthinellum Biourge (1 strain), Penicillium oxalicum Currie & Thom (1 strain). The strains belong to order Eurotiales, Class Plectomycetes, phylum Ascomycota. One strain showed ITS rDNA sequence similarity to Pseudocercospora sp. (1 strain). The strain belongs to order Capnodiales, class Dotthideomycetes, phylum Ascomycota. Three strains (Penicillium sp. FIB.PRI.6.1, Fraseriella sp. FIB.PRI.6.2, and mycelia sterilia FIB.PRII.3) were unable to be identified to species level."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2013
S46009
UI - Skripsi Membership  Universitas Indonesia Library
cover
Teguh Prasetia Teja
Identifikasi kapang dari manuskrip berbahan kertas eropa berdasarkan analisis sekuens daerah its pada rDNA = Identification of moulds from old european paper manuscripts based on sequence analysis of rDNA its region
"Penelitian ini bertujuan untuk mengidentifikasi 16 strain kapang dari tujuh manuskrip kuno berbahan kertas Eropa dengan analisis sekuens daerah internal transcribed spacers (ITS) pada rDNA dan melakukan deskripsi morfologi terhadap kapang-kapang tersebut. Kapang tersebut berasal dari manuskrip kuno asal Keraton Kasepuhan Cirebon dan Perpustakaan Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia (FIB UI). Amplifikasi daerah ITS rDNA menggunakan forward primer ITS 1 dan reverse primer ITS 4. Deskripsi morfologi dilakukan pada medium Czapek’s Dox Agar (CDA). Panjang fragmen daerah ITS berdasarkan elektroforesis gel dari kapang genus Aspergillus adalah 500--800 pb, Penicillium 500--600 pb, dan mycelia sterilia 550--800 pb. Satu strain kapang merupakan anggota filum Ascomycota, kelas Loculoascomycetes,ordo Dothideales, famili Davidielaceae, dan memiliki homologi sekuens daerah ITS dengan spesies terdekatnya, yaitu Cladosporium cladosporioides (Fresen.).
Sebanyak 13 strain merupakan anggota filum Ascomycota, kelas Plectomycetes,ordo Eurotiales, famili Trichocomaceae, dan memiliki homologi sekuens daerah ITS dengan spesies terdekatnya, yaitu Aspergillus flavus Link. (satu strain),Aspergillus oryzae Cohn. (dua strain), Aspergillus niger (dua strain), Penicillium citrinum Thom (empat strain), Penicillium griseofulvum Dreckx. (satu strain),Penicillium janthinellum Biourd (satu strain), Eurotium amstelodami L. Mangin (satu strain), dan Eurotium rubrum Jos. Konig. (satu strain). Dua strain kapang lainnya, yaitu satu strain Aspergillus sp. dan satu strain Penicillium sp. belum berhasil diidentifikasi hingga tingkat spesies.
The aim of this research was to identify 16 strains of moulds from seven old European paper manuscripts based on sequence analysis of ITS region rDNA and to describe their morphology. The moulds were isolated from old manuscripts from Keraton Kasepuhan Cirebon and the library of Faculty of Humanities University of Indonesia. ITS 1 and ITS 4 primers were used as forward and reverse primers for PCR, respectively. The mould’s morphology was examined on Czapek’s Dox Agar (CDA). The lengths of ITS region of genus Aspergillus based on gel electrophoresis were on the range of 500--800 bp, Penicillium 500--600 bp, and mycelia sterilia 550--800 bp. One strain belongs to phylum Ascomycota, class Loculoascomycetes, order Dothideales, family Davidielaceae,and showed ITS region similarity to Cladosporium cladosporioides (Fresen.).
Thirteen strains belong to phylum Ascomycota, class Plectomycetes, order Eurotiales, family Trichocomaceae, and showed ITS region similarities to Aspergillus flavus Link. (one strain), Aspergillus oryzae Cohn. (two strains),Aspergillus niger (two strains), Penicillium citrinum Thom (four strains),Penicillium griseofulvum Dreckx. (one strain), Penicillium janthinellum Biourd (one strain), Eurotium amstelodami L. Mangin (one strain), and Eurotium rubrum Jos. Konig. (one strain). One strain of Aspergillus sp. and one strain of Penicillium sp. were unable to be identified to species level."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2013
S46010
UI - Skripsi Membership  Universitas Indonesia Library
cover
Eka Nurhangga
Identifikasi Khamir dari Saluran Pencernaan Lebah Pejantan dan Lebah Pekerja Pengumpul Polen Apis mellifera L. Berdasarkan Data Sequence Daerah ITS rDNA = Identification of Yeasts from Digestive Track of Drone and Pollen Collecting Bees of Apis mellifera L. Based on ITS Region of rDNA Sequence Data
"Penelitian bertujuan untuk mengetahui identitas khamir dari saluran pencernaan lebah madu Apis mellifera L. yang mengunjungi bunga kapuk (Ceiba pentandra (L.) Gaertn) di Jepara. Sebanyak 12 isolat khamir yang terdiri atas 3 isolat dari saluran pencernaan lebah pejantan (drone) dan 9 isolat dari lebah pekerja pengumpul polen (pollen collecting bees, PCB) diidentifikasi berdasarkan data sequence daerah internal transcribed spacer (ITS) rDNA. Primer yang digunakan untuk amplifikasi daerah ITS adalah primer forward ITS1 atau primer reverse ITS4. Hasil elektroforesis produk PCR menunjukkan daerah ITS rDNA khamirkhamir tersebut bervariasi antara 400 pb hingga 750 pb. Berdasarkan hasil pencarian homologi sequence daerah ITS rDNA melalui program basic local alignment search tool (BLAST), analisis filogenetik dengan metode Neighbor Joining, dan pengamatan karakter morfologi, 12 isolat tersebut diidentifikasi ke dalam empat genus dan tujuh spesies. Berdasarkan taksonomi, khamir-khamir tersebut termasuk kedalam family Candidaceae dan Saccharomycetaceae, order Saccharomycetales, class Hemiascomycetes dari phylum Ascomycota. Isolat-isolat tersebut diidentifikasi ke dalam spesies Candida magnoliae (isolat JZ078), C. orthopsilosis (isolat JZ068 dan JZ069), C. parapsilosis (isolat JZ067 dan JZ095), Debaryomyces hansenii (isolat JZ083 dan JZ096), Meyerozyma caribbica (isolat JZ094), Zygosaccharomyces mellis (isolat JZ075), dan Z. siamensis (isolat JZ054,JZ055, dan JZ056).
The aim of this research was to determine the identity of the yeasts isolated from the digestive tract of honey bee Apis mellifera that visit flowers of kapok (Ceiba pentandra (L.) Gaertn) in Jepara. A total of 12 yeast isolates consist of 3 isolates from digestive tract of drones and 9 isolates from digestive tract of pollen collecting bees (PCB) were identified based on sequence data of internal transcribed spacers regions of ribosomal DNA (ITS rDNA). The primer set of ITS1 (forward primer) and ITS4 (reverse primer) were used to amplify the ITS rDNA of yeasts. The results of electrophoresis of PCR products showed ITS region rDNA of yeast isolates varied between 400bp--750 bp. Based on sequence homology search results by basic local alignment search tool (BLAST) program, phylogenetic analysis by Neighbor Joining method, and morphological characterization, those 12 isolates were identified into four genera and seven species. Taxonomically, 11 isolates belong to family Candidaceae and Saccharomycetaceae, order Saccharomycetales, class Hemiascomycetes of the phylum Ascomycota. Those isolates were identified as species Candida magnoliae (isolat JZ078), C. orthopsilosis (isolat JZ068 and JZ069), C. parapsilosis (isolat JZ067 and JZ095), Debaryomyces hansenii (isolat JZ083 and JZ096), Meyerozyma caribbica (isolat JZ094), Zygosaccharomyces mellis (isolat JZ075), and Z. siamensis (isolat JZ054, JZ055, and JZ056)."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2013
S47074
UI - Skripsi Membership  Universitas Indonesia Library
cover
Ana Khoirotun Nisa
Identifikasi khamir dari saluran pencernaan lebah pekerja pengumpul nektar apis mellifera l berdasarkan data sequence daerah its rDNA = Identification of yeasts from the digestive tracts of nectar collecting bees of apis mellifera l based on its region of rDNA sequence data
"Penelitian bertujuan mengetahui identitas khamir dari saluran pencernaan lebah pekerja pengumpul nektar (nectar collecting bee, NCB) Apis mellifera yang mengunjungi bunga kapuk (Ceiba pentandra) di Jepara. Sebanyak 12 isolat khamir diidentifikasi berdasarkan data sequence daerah Internal Transcribed Spacers (ITS) rDNA. Primer forward ITS1 dan primer reverse ITS4 digunakan untuk amplifikasi daerah ITS rDNA. Hasil elektroforesis produk PCR menunjukkan ukuran daerah ITS rDNA isolat khamir-khamir tersebut bervariasi antara 400 pb hingga 800 pb. Berdasarkan hasil pencarian homologi sequence daerah ITS rDNA m2elalui program Basic Local Alignment Search Tool (BLAST), analisis filogenetik dengan metode Neighbor Joining, dan pengamatan karakter morfologi, 12 isolat khamir tersebut diidentifikasi ke dalam empat genus dan lima spesies. Berdasarkan taksonomi, 11 isolat khamir termasuk ke dalam anggota order Sacharomycetales, class Hemiascomycetes dari phylum Ascomycota dan satu isolat termasuk ke dalam anggota order Ustilaginales, class Ustilaginomycetes dari phylum Basidiomycota. Isolat-isolat tersebut diidentifikasi sebagai Candida parapsilosis (JZ102, JZ105, JZ116, dan JZ121), Candida rugosa (JZ117, JZ121), Debaryomyces hansenii (JZ100, JZ113, JZ118), Meyerozyma caribbica (JZ124, JZ125), dan Pseudozyma sp. (JZ104).
The aim of this study was to identify the yeast isolates from the digestive tracts of nectar collecting bees (NCB) of Apis mellifera. A total of 12 yeast isolates from the digestive tracts of NCB foraging on flowers of Ceiba pentandra in Jepara, Central Java, were identified based on sequence data of Internal Transcribed Spacers Regions of ribosomal DNA (ITS rDNA). The primer set of ITS1 (forward primer) and ITS4 (reverse primer) were used to amplify the ITS rDNA of the isolates. Gel electrophoresis results showed that the size of ITS region rDNA of the isolates were varied on the range of 400 bp -- 800 bp. Based on sequence homology search results by Basic Local Alignment Search Tool (BLAST) program, phylogenetic analysis by Neighbor Joining method, and morphological characterization, those 12 isolates were identified into four genera and five species. Taxonomically, 11 isolates belong to order Sacharomycetales, class Hemiascomycetes from the phylum Ascomycota and 1 isolate belongs to order Ustilaginales, class Ustilaginomycetes from the phylum Basidiomycota. Those isolates were identified as Candida parapsilosis (JZ102, JZ105, JZ116, and JZ121), Candida rugosa (JZ117, JZ121), Debaryomyces hansenii (JZ100, JZ113, JZ118), Meyerozyma caribbica (JZ124, JZ125), and Pseudozyma sp. (JZ104). "
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2013
S53334
UI - Skripsi Membership  Universitas Indonesia Library
cover
Dyah Restu Pamuji
Identifikasi Khamir dari Saluran Pencernaan Lebah Pekerja Pengumpul Polen Apis mellifera L. Berdasarkan Data Sequence Daerah ITS rDNA = Identification of Yeasts Isolated from Digestive Tract of Pollen Collecting Bees Apis mellifera L. Based on ITS Region rDNA Sequence Data
"Penelitian bertujuan mengetahui identitas khamir dari saluran pencernaan lebah pekerja pengumpul polen (pollen collecting bees, PCB) Apis mellifera. Sebanyak 12 isolat khamir dari saluran pencernaan PCB yang mengunjungi bunga kapuk Ceiba pentandra di Jepara, Jawa Tengah, diidentifikasi berdasarkan data sequence daerah internal transcribed spacer (ITS) rDNA. Amplifikasi daerah ITS rDNA menggunakan primer forward ITS1 dan primer reverse ITS4. Elektroforesis produk PCR menunjukkan bahwa daerah ITS rDNA khamir-khamir tersebut berukuran antara 400--900 pb. Berdasarkan hasil pencarian homologi sequence menggunakan program basic local alignment search tool (BLAST), analisis filogenetik menggunakan metode Neighbor Joining (NJ), dan karakterisasi morfologi, 12 isolat khamir tersebut terdiri dari tujuh spesies yang termasuk dalam lima genus. Secara taksonomi, seluruh khamir tersebut termasuk phylum Ascomycota, class Hemiascomycetes, dan order Saccharomycetales. Isolat-isolat tersebut diidentifikasi sebagai Candida magnoliae (isolat JZ002), Candida orthopsilosis (isolat JZ003, JZ008, JZ011, dan JZ034), Candida rugosa (isolat JZ010); Debaryomyces hansenii (isolat JZ001); Meyerozyma caribbica (isolat JZ013 dan JZ014), Pichia guilliermondii (isolat JZ015), dan Zygosaccharomyces siamensis (isolat JZ005 dan JZ006).
The aim of this study was to obtain the identity of yeasts from digestive tracts of pollen collecting bees (PCB) Apis mellifera. A total of 12 yeast isolates obtained from digestive tract of PCB foraging on flowers Ceiba pentandra in Jepara, Central Java, were identified based on sequence data of internal transcribed spacer of ribosomal DNA (ITS rDNA). The primer set of ITS1 (forward primer) and ITS4 (reverse primer) were used to amplify the ITS region rDNA. Gel electrophoresis result showed that the size of ITS rDNA of those yeast were varied between 400--900 base pairs. Based on sequence homology search using basic local alignment search tool (BLAST) program, phylogenetic analysis by Neighbor Joining method, and morphological characterization, those 12 isolates belong to five genera and seven species. Taxonomically, all of those isolates belong to order Saccharomycetales, class Hemiascomycetes from the phylum Ascomycota. Those 12 isolates were identified as species Candida magnoliae (isolate JZ002); Candida orthopsilosis (isolates JZ003, JZ008, JZ011, and JZ034); Candida rugosa (isolate JZ010); Debaryomyces hansenii (isolate JZ001); Meyerozyma caribbica (isolates JZ013 and JZ014); Pichia guilliermondii (isolate JZ015); and Zygosaccharomyces siamensis (isolates JZ005 and JZ006)."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2013
S46972
UI - Skripsi Membership  Universitas Indonesia Library
cover
Annisa Dwi Aprilina
Pengujian kemampuan kapang dari manuskrip Dluwang Lama asal Cirebon menggunakan kertas merang sebagai substrat = Evaluation of moulds from Old Dluwang Manuscripts from Cirebon to utilize rice straw paper as a substrate.
"Kapang menyebabkan deteriorasi pada manuskrip dluwang lama di Indonesia. Penelitian bertujuan untuk mengetahui kemampuan kapang asal manuskrip dluwang lama dari Cirebon (Keraton Kasepuhan dan Mertasinga) dalam menggunakan kertas merang sebagai substrat. Deskripsi empat strain kapang yang telah diidentifikasi secara molekuler pada penelitian sebelumnya, dikonfirmasi berdasarkan karakterisasi morfologi pada Potato Dextrose Agar (PDA) dan Malt Extract Agar (MEA). Biakan kapang dalam PDA miring di suhu 26,5 oC, umur 7 hari digunakan untuk pembuatan suspensi sel dalam akuades steril 5 ml. Tiga ml suspensi sel diinokulasikan ke dalam 27 ml Czapek Dox Broth (CDB) tanpa sumber karbon dengan penambahan kertas merang (diameter 5,2 cm) sebagai substrat, dan pada 27 ml CDB tanpa kertas merang sebagai kontrol, inkubasi di suhu ruang (28 oC), selama 30 hari. Hasil karakterisasi morfologi mengkonfirmasi empat strain kapang adalah Penicillium rubens Biourge UICC 1062, Aspergillus jensenii Jurjević, S.W. Peterson & B.W. Horn UICC 1069, Cladosporium colocasiae Sawada UICC 1071, dan Eurotium rubrum Jos. König, E. Spieckermann & W. Bremer UICC 1006. Semua kapang menggunakan kertas merang sebagai substrat untuk sumber karbon dan nutrien berdasarkan adanya pertumbuhan (hifa dan sporulasi), perubahan kondisi kertas (kertas menjadi robek, rapuh, adanya titik sporulasi berwarna kehijauan dan cokelat kehitaman), dan persentase pengurangan berat kering kertas (3,44--15,92%).
Moulds causes deterioration on old dluwang manuscripts in Indonesia. This study aims to determine the ability of moulds from the old dluwang manuscripts from Cirebon (Keraton Kasepuhan and Mertasinga) in using rice straw paper as a substrate. Four mould strains which were identified by molecular method in previous study, were described to confirm their species identities based on morphology characterisation on Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA). Seven days-old mould cultures in PDA slants, at 26.5 oC, were used for cell suspensions in 5 ml sterile water. Three ml cell suspensions were inoculated into 27 ml Czapek Dox Broth (CDB) without a carbon source with the addition of rice straw paper (5.2 cm in diameter) as a substrate, and into 27 ml CDB without a carbon source and rice straw paper as a control, incubated at room temperature (28 oC), for 30 days. The results showed that description of four mould strains was confirmed as Penicillium rubens Biourge UICC 1062, Aspergillus jensenii Jurjević, S.W. Peterson & B.W. Horn UICC 1069, Cladosporium colocasiae Sawada UICC 1071, and Eurotium rubrum Jos. König, E. Spieckermann & W. Bremer UICC 1006. All mould strains were able to use rice straw paper as a substrate to obtain carbon source and nutrient for growth based on the presence of hyphae and sporulation, changes in paper conditions (tear, fragile, sporulation spots in light green and brownish-black) and percentage of paper dry weight loss (3.44--15.92%)."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2021
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
cover
Alvin Natalius
Isolasi, identifikasi, dan penapisan kapang selulolitik dari manuskrip kuno kertas Eropa asal Perpustakaan Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia = Isolation, identification, and screening of cellulolytic fungi from old manuscripts of European papers from the Library of Faculty of Humanities of Universitas Indonesia
"Penelitian bertujuan mengisolasi kapang manuskrip kuno kertas Eropa asal Perpustakaan Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia, mengetahui kemampuan kapang-kapang tersebut tumbuh pada kertas Eropa dan kemampuan selulolitiknya, serta mengidentifikasinya. Hasil isolasi dan pemurnian pada medium DG 18 menghasilkan 11 isolat kapang. Penapisan isolatisolat menunjukkan 9 isolat memiliki kemampuan tumbuh pada substrat kertas Eropa. Penapisan menggunakan Carboxymethyl Cellulose (CMC) dan Congo red memberikan indikasi delapan isolat memiliki enzim selulase berupa endoglukanase.
Hasil identifikasi konvensional berdasarkan karakter morfologi menunjukkan kapang-kapang tersebut adalah Aspergillus E.FIB.UI.1.1.2, Aspergillus E.FIB.UI.1.2, Aspergillus E.FIB.UI.4.2, Aspergillus E.FIB.UI.5.3, Penicillium E.FIB.UI.2.1.1, Penicillium E.FIB.UI.2.1.2, Penicillium E.FIB.UI.2.8, mycelia sterilia E.FIB.UI.1.1.1, dan mycelia sterilia E.FIB.UI.3.3.
This research was to isolate fungi from the Library of Faculty of Humanities of Universitas Indonesia, to screen cellulolytic isolates that grow on old manuscripts of European papers and to identify the isolates. Eleven mould isolates were obtained on medium DG 18 Agar. Nine isolates were able to grow on European papers. Eight isolates were able to grow on Carboxymethyl Cellulose (CMC) and Congo red indicating they have endoglucanase.
Identification by conventional method showed that they were Aspergillus E.FIB.UI.1.1.2, Aspergillus E.FIB.UI.1.2, Aspergillus E.FIB.UI.4.2, Aspergillus E.FIB.UI.5.3, Penicillium E.FIB.UI.2.1.1, Penicillium E.FIB.UI.2.1.2, Penicillium E.FIB.UI.2.8, mycelia sterilia E.FIB.UI.1.1.1, and mycelia sterilia E.FIB.UI.3.3."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2012
S42963
UI - Skripsi Open  Universitas Indonesia Library
cover
Michelle
Isolasi, identifikasi, dan pengujian kemampuan kapang selulolitik dari manuskrip kuno berbahan daluang asal Perpustakaan Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia = Isolation, identification, and investigation of cellulolytic fungi from old manuscripts of daluang materials from Library of Faculty of Humanities University of Indonesia
"Penelitian bertujuan untuk mengisolasi, mengidentifikasi, dan menguji kemampuan kapang selulolitik dari lima manuskrip daluang asal Perpustakaan FIB UI. Hasil isolasi pada medium PCA menghasilkan 19 isolat kapang, sedangkan isolasi pada medium DG18 menghasilkan 15 isolat kapang xerofilik. Sebanyak 15 isolat kapang memiliki kemampuan tumbuh pada kertas daluang, sedangkan 14 isolat dapat menggunakan CarboxyMethyl Cellulose (CMC) dan Congo red yang mengindikasikan dapat menghasilkan endoglukanase. Hasil identifikasi konvensional berdasarkan karakter morfologi menunjukkan 4 isolat merupakan genus Aspergillus, 8 isolat merupakan genus Penicillium, 1 isolat merupakan genus Fraseriella, dan 2 isolat merupakan mycelia sterilia.
This research was to isolate fungi from old daluang manuscripts from Library of Faculty of Humanities University of Indonesia, to investigate cellulolytic isolates and to identify the isolates. Nineteen mould isolates were obtained on medium PCA, whilst fifteen xerophilic mould isolates were obtained on medium DG18 agar. Fifteen isolates were able to grow on daluang paper. Fourteen isolates were able to grow on Carboxymethyl Cellulose (CMC) and Congo red indicating they have endoglucanase. Identification by conventional method showed that 4 isolates were Aspergillus, 8 isolates were Penicillium, 1 isolate were Fraseriella, and 2 isolates were mycelia sterilia."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2012
S43553
UI - Skripsi Open  Universitas Indonesia Library
<<   1 2 3 4 5 6 7 8 9 10   >>