Hasil Pencarian  ::  Simpan CSV :: Kembali

"Latar Belakang: Propolis fluoride salah satu sediaan yang dapat menghambat perkembangan bakteri penyebab karies. Tujuan: Menganalisis pengaruh propolis fluoride terhadap viabilitas biofilm S. mutans dalam berbagai fase. Metode: Model biofilm S.mutans di inkubasi selama 4 jam fase adesi, 12 jam fase akumulasi aktif, dan 24 jam fase maturasi, kemudian dipaparkan dengan propolis fluoride 3,3 ; 6,6, 10 kelompok perlakuan, dan SDF 38 kelompok kontrol. Analisis Viabilitas biofilm S.mutans dilakukan dengan uji MTT untuk dibaca pada microplate reader. Hasil: Pada pemaparan Propolis 3,3, persentase viabilitas biofilm S.mutans pada fase adesi 14,89 3,19; fase akumulasi aktif 24,37 7,43; dan fase maturasi 21,35 3,06. Pada pemaparan Propolis 6,6, persentase viabilitas biofilm S.mutans pada fase adesi 10,10 2,43; fase akumulasi aktif 20,88 13,17; dan fase maturasi 18,82 4,51. Pada pemaparan Propolis 10, persentase viabilitas biofilm S.mutans pada fase adesi8,04 1,59; fase akumulasi aktif 11,17 8,90; dan fase maturasi 16,75 1,83. Kesimpulan: Propolis fluoride 10 dapat menurunkan viabilitas biofilm S.mutans pada fase adesi.

---

Background: Propolis fluoride in one of dosage could inhibit the growth of bacteria that cause caries.

Objective: To analyze the effect of propolis fluoride on the viability of S. mutans biofilm in various phases.

Method: S. mutans biofilm models were incubated for 4 hours adhesion phase, 12 hours active accumulation phase, and 24 hours maturation phase, then presented with propolis fluoride 3.3 6.6, 10 treatment group, and SDF 38 control group. Analysis of S. mutans biofilm viability is tested by MTT in the microplate reader.

Results: Exposure of Propolis Flouride 3.3, the percentage of S. mutans biofilm viability in the adhesion phase is 14.89 3.19 active accumulation phase is 24.37 7.43 and the maturation phase is 21.35 3.06. On exposure of Propolis Flouride 6.6, the percentage of S. mutans biofilm viability in adhesion phase is 10,10 2,43 active accumulation phase is 20.88 13.17 and the maturation phase is 18.82 4.51. On exposure of Propolis Fluoride 10, the percentage of S. mutans biofilm viability in the phase of adhesion is 8.04 1.59 active accumulation phase is 11.17 8.90 and the phase of maturation is 16.75 1.83.

Conclusion: Propolis fluoride 10 could reduced the viability of S. mutans biofilm in adhesion phase."

"Latar Belakang: Nano Silver Fluoride NSF memiliki efek antibakteri terhadap Streptococcus mutans bakteri penyebab karies. Tujuan: Menganalisis pengaruh NSF terhadap viabilitas S.mutans dalam berbagai fase pembentukan biofilm. Metode: Biofilm S.mutans diinkubasi selama 4 jam fase adhesi, 12 jam fase akumulasi aktif dan 24 jam fase maturasi pada suhu 37 C. Ketiga model biofilm dipapar NSF dengan konsentrasi Ag 0,4 F- 2,26, Ag 0,9 F- 2,26, Ag 1,4 F- 2,26, Ag 1,9 F- 2,26 selama 1 jam. Persentase viabilitas dinilai dengan menggunakan MTT assay. Hasil: Tidak ada perbedaan bermakna p>0,05 antara viabilitas biofilm pada fase adhesi, fase akumulasi aktif, ataupun fase maturasi. Kesimpulan: NSF mampu menurunkan viabilitas biofilm S.mutans dalam berbagai fase pembentukan.

---

Background: Nano Silver Fluoride NSF has antibacterial effect against Streptococcus mutans that cause dental caries.

Objective: To analyze the effect of NSF on the viability of S.mutans in various phases of biofilm formation.

Methods: S.mutans biofilm was incubated for 4 hours adherence phase, 12 hours active accumulation phase and 24 hours maturation phase at 37 C then exposed by NSF at concentration Ag 0,4 F 2,26, Ag 0,9 F 2,26, Ag 1,4 F 2,26, Ag 1,9 F 2,26 for 1 hour. The percentage of viability was tested with MTT assay.

Result: Biofilm viability of S.mutans in various phases showed no significant difference p 0,05.

Conclusion: NSF can reduce the viability of S.mutans in various phases of biofilm formation."

"Latar Belakang: Silver Diamine Fluoride SDF merupakan agen antibakteri yang mempunyai efek samping yaitu timbulnya noda kehitaman pada permukaan gigi. Propolis Fluoride PPF dikenal sebagai agen antibakteri yang dapat menggantikan SDF tanpa memiliki efek samping yang sama. Tujuan: Menganalisis pengaruh PPF dalam menghambat pembentukan biofilm S. mutans dan S. gordonii serta dibandingkan dengan SDF, dengan menggunakan uji MTT. Metode: Suspensi bakteri S. mutans dan S. gordonii dalam media BHI yang diperkaya sukrosa 0,2 dipaparkan PPF. Kemudian diinkubasi pada 4 waktu berbeda yaitu selama 4 jam, 12 jam, 24 jam, dan 48 jam. Presentase inhibisi dianalisis menggunakan Uji MTT. Hasil: Tidak ada perbedaan bermakna p>0,05 antara inhibisi S. mutans dan S. gordonii dalam berbagai waktu. Kesimpulan: PPF memiliki potensi yang sama dengan SDF dalam menghambat pembentukan biofilm S. mutans dan S. gordonii.

---

Background: Silver Diamine Fluoride SDF is an antibacterial agent that has a side effect like black stain appearance on the surface of the teeth. Propolis Fluoride PPF is an antibacterial agent which can replace SDF without having the same side effect.

Purpose: To analyze the effect of PPF in inhibiting biofilm formation of S. mutans dan S. gordonii in comprasion to SDF, by using MTT assay.

Methods: Bacterial suspension of S. mutans and S. gordonii was put inside the BHI medium that enriched with 0,2 sucrose and exposed to PPF, then incubated at 4 different times for 4 hours, 12 hours, 24 hours, and 48 hours. The percentage of inhibition was tested with MTT assay.

Result: There was no significant difference p 0,05 between inhibition of S. mutans and S. gordonii in various time.

Conclusion: PPF has the same potention as SDF in inhibiting biofilm formation of S. mutans and S. gordonii."

"Latar Belakang: Perkembangan karies gigi berkaitan dengan bakteri Streptococcus mutans atau Streptococcus gordonii. Propolis dilaporkan sebagai agen antibakteri karena mengandung flavonoid berupa apigenin dan tt-farnesol yang dapat menghambat aktivitas enzim glukosiltransferase dan mempengaruhi integritas membran bakteri. Tujuan: Menganalisis potensi hambat pasta gigi mengandung ekstrak propolis UI terhadap pembentukan biofilm Streptococcus mutans atau Streptococcus gordonii. Metode: Biofilm Streptococcus mutans atau Streptococcus gordonii yang telah dipaparkan pasta gigi ekstrak propolis UI dengan  konsentrasi 25mg/10ml, 50mg/10ml, dan 100mg/10ml kemudian diinkubasi selama 4 jam (fase adhesi), 12 jam (fase akumulasi aktif) dan 24 jam (fase maturasi) pada suhu 37ºC. Persentase inhibisi dinilai dengan menggunakan MTT assay. Hasil: Persentase potensi hambat biofilm Streptococcus mutans tertinggi setelah inkubasi 12 jam dan Streptococcus gordonii setelah inkubasi 4 jam dengan konsentrasi 100mg/10 ml.  Kesimpulan: Efek paparan pasta gigi mengandung ekstrak propolis UI dalam menghambat pembentukan biofilm Streptococcus mutans atau Streptococcus gordonii dipengaruhi oleh konsentrasi dan durasi paparan pasta gigi UI.

---

Background: The development of dental caries has been found to be associated with Streptococcus mutans and Streptococcus gordonii. Propolis has been reported as a potent antimicrobial material because containing flavonoids such as apigenin and tt-farnesol that inhibit glucosyltransferase enzyme activity and membrane integrity.

Objective: To analyze the effect of toothpaste containing propolis wax UI in inhibit Streptococcus mutans or Streptococcus gordonii biofilm formation.

Methods: Streptococcus mutans and Streptococcus gordonii biofilm that has been exposed by propolis UI toothpaste at concentration 25mg/10ml, 50mg/10ml, dan 100mg/10ml was incubated for 4 hours (adherence phase), 12 hours (active accumulation phase) and 24 hours (maturation phase) at 37ºC. The percentage of inhibition was tested with MTT assay.

Result: Inhibition percentage of Streptococcus mutans the highest is on active accumulation phase and Streptococcus gordonii biofilm is on adherence phase at concentraton 100mg/10ml.

Conclusion: Propolis UI toothpaste effect on inhibiting biofilm formation of Streptococcus mutans or Streptococcus gordonii depend on concentration and duration of time."

"Latar belakang : S. mutans merupakan patogen utama penyebab karies. NSF diketahui memiliki sifat antibakterial. Tujuan : Menganalisis pengaruh NSF dalam menghambat virulensi dan pembentukkan biofilm S. mutans. Metode : Suspensi bakteri S. mutans dalam media BHI yang diperkaya sukrosa 0.2 dipaparkan NSF diinkubasi selama 20 jam. Persen inhibisi biofilm dinilai menggunakan uji crystal violet. Hasil : Nilai KHM NSF adalah 2.66 dan nilai KBM 4.16 . NSF mampu menghambat pembentukkan biofilm S. mutans. Kesimpulan : NSF mampu menghambat virulensi dan pembentukkan biofilm S. mutans.

---

Background: S. mutans are the primary pathogens that cause caries. NSF known to have antimicrobial properties.

Aim: To analyze the effect of NSF in inhibiting virulence and biofilm formation of S. mutans.

Methods: Bacterial suspension of S. mutans in BHI medium enriched 0.2 sucrose exposed with NSF incubated for 20 hours. Percent inhibition of biofilm was assessed using crystal violet test.

Result: NSF MIC value is 2.66 and MBC value is 4.16 . NSF is able to inhibit biofilm formation of S. mutans.

Conclusion: NSF is able to inhibit virulence and biofilm formation of S.mutans. "

"Latar Belakang: Pasta gigi merupakan bahan semi-aqueous yang digunakan untuk menjaga kebersihan gigi dan mulut. Pada umumnya, zat aktif yang dikandung dalam pasta gigi terbuat dari bahan sintetik. Namun, bahan tersebut dapat menimbulkan efek samping sehingga dibutuhkan bahan dengan efek samping yang lebih sedikit. Propolis merupakan suatu substansi resin yang memiliki sifat antibakterial dan aman terhadap tubuh sehingga dapat digunakan sebagai pengganti bahan sintetik yang dapat meminimalkan efek samping yang terjadi. Tujuan Penelitian: Menganalisis pengaruh paparan pasta gigi ekstrak propolis dan pasta gigi non-propolis terhadap pertumbuhan biofilm S. mutans ATCC 25175. Metode Penelitian: Pembentukan biofilm dilakukan dengan kultur S. mutans ATCC 25175 ke dalam well yang telah berisi pelikel. Pasta gigi ekstrak propolis dan non-propolis dimasukkan ke dalam well, serta diinkubasi selama 3 jam dan 18 jam. Uji kristal violet dan uji OpenCFU dilakukan untuk mengevaluasi total biomassa. Hasil Penelitian: Pada inkubasi 3 jam, tidak terlihat adanya penurunan biomassa baik pada pasta gigi ekstrak propolis maupun non-propolis. Namun, penurunan biomassa terlihat pada kedua jenis pasta gigi setelah inkubasi 18 jam. Kesimpulan: Paparan pasta gigi ekstrak propolis dalam durasi yang lama mampu menurunkan biomassa S. mutans ATCC 25175 dan menghambat pembentukan biofilm.

---

Background: Toothpaste was a semi-aqueous material that is used to maintain dental and oral hygiene. Commonly, the active ingredients contained in toothpastes were made from synthetic ingredients. However, these ingredients may causes several adverse effect, therefore a biocompatible ingredient is required to minimize the adverse effect. Propolis is a resin substance that has antibacterial properties and non-toxic to the body. Thus, propolis can be used as an active substance in toothpaste to minimize the adverse effects caused by synthetic toothpaste.

Aim: To analyze the effect of propolis extract and nonpropolis toothpaste exposure toward S. mutans biofilm ATCC 25175 growth.

Method: S. mutans ATCC 25175 culture was added to the well that already contained pellicle to make biofilm. Both toothpaste was added into the well and incubated for 3 h and 18 h. Crystal violet and OpenCFU method was used to measure total biomass of S. mutans ATCC 25175 biofilm.

Result: After 3 hours incubation, there was no reduction in biomass in both toothpaste. However, biomass reduction was seen in both toothpaste after incubation of 18 hours.

Conclusion: Long duration of exposure to propolis extract toothpaste may reduce biomass of S. mutans ATCC 25175 and inhibit biofilm formation."

"Latar Belakang : Karies gigi merupakan penyakit gigi dan mulut yang sering ditemukan di Indonesia. Diperlukan upaya alternatif pencegahan, yang dalam penelitian ini dilakukan dengan menggabungkan dua bahan aktif CPP-ACP dan lilin propolis dalam satu sediaan permen karet bebas gula dengan lima konsentrasi yang berbeda (0% Prop, 0% CPP-ACP ; 0% Prop + CPP-ACP ; 2% Prop + CPPACP ; 4% Prop + CPP-ACP ; dan 6% Prop + CPP-ACP).Tujuan : Menganalisis kadar pelepasan ion kalsium dan fosfat oleh CPP-ACP untuk mendukung remineralisasi dan keefektifan lilin propolis dalam menekan pembentukan massa biofilm S.mutans pada subjek bebas karies serta melihat apakah kedua bahan aktif ini efektif jika digabungkan dalam satu sediaan permen karet bebas gula.Metode : 25 sampel saliva bebas karies sebelum dan sesudah simulasi pengunyahan lima konsentrasi permen karet in vitro dilakukan uji pelepasan ion kalsium dan fosfat serta uji biofilm. Pelepasan ion kalsium dideteksi menggunakan AAS, ion fosfat menggunakan Spektrofotometri UV-VIS, dan uji biofilm menggunakan uji crystal violet 96-well plate ELISA dan dibaca menggunakan microplate reader.Hasil : Permen karet CPP-ACP-Propolis dengan konsentrasi 0% Prop + CPP-ACP menunjukkan kadar pelepasan ion kalsium (p≤0,05) dan fosfat (p>0,05) tertinggi dan signifikan dalam menekan pembentukan massa biofilm S.mutans (p≤0,05).Simpulan : Terjadi peningkatan kadar ion kalsium dan fosfat pada saliva bebas karies, serta, penurunan massa biofilm S.mutans setelah pengunyahan permen karet CPP-ACP-Propolis.

---

Background: Dental caries is an oral disease commonly found in Indonesia. Alternative prevention are needed, which in this research is going to be combining two active components which are CPP-ACP and propolis wax into one substance of sugar-free chewing gum with five different concentrations (0% Prop, 0% CPPACP ; 0% Prop + CPP-ACP ; 2% Prop + CPP-ACP ; 4% Prop + CPP-ACP ; and 6% Prop + CPP-ACP).Objectives: To analyze the amount of calcium and phosphate ion released by CPP-ACP to support the remineralization and to analyze the effectiveness of propolis wax in suppressing the mass formation of Streptococcus mutans biofilm in caries-free subjects and also observing if these two active components are effective when combined into one substance of sugarfree chewing gum.Methods: 25 samples of caries-free saliva before and after the mastication simulation (five concentrations of chewing gum) in vitro, observed the release of calcium and phosphate ion along with a biofilm assay. The release of calcium ion is detected using AAS, while phosphate ion using the Spectrophotometry UV-VIS, and the biofilm assay using the crystal violet 96-well plate ELISA and evaluated with a microplate reader.Result: Chewing gum with a concentration of 0% Prop + CPP-ACP has shown the highest release level of calcium (p<0,05) and phosphate ion (p>0,05) and is significant in suppressing the mass formation of S.mutans biofilm (p<0,05).Conclusion: Increased calcium and phosphate ion level in caries-free saliva and decreased of S.mutans biofilm mass after mastication simulation of CPP-ACP-Propolis chewing gum are detected."

"Latar Belakang : Ekstrak kismis telah dikenal sejak dahulu dalam menghambat pertumbuhan bakteri patogen, karena mengandung oleanolic acid yang telah terbukti dapat menghambat pertumbuhan bakteri rongga mulut.Tujuan: Penelitian ini bertujuan untuk membuktikan efek antimikroba infusum Kismis terhadap Streptococcus mutans.Metode: Infusum Kismis dibuat dengan proses pemanasan 100oCselama 15 menit pada 50 gr kismis dalam 500ml air (konsentyrasi 10%), kemudian diopanaskan lagi sehingga larutan tersisa 50ml (konsentrasi 100%). Untuk penelitian ini dibuat infusum 80%, 60%, 40%, 30%, dan 15% sesuai dengan prosedur yang benar. Efek antimikroba masing2 infusum kismis diperiksa dengan metode dilusi sehingga diperoleh nilai KHM dan KBM serta metode difusi sehingga diperoleh nilai Zona Hambatan terhadap 6 koloni streptococcus mutans.Hasil: Efek infusum Kismis terhadap Streptococcus mutans adalah sebagai berikut : Pada koloni 1 : zona hambatan 1,00 mm; KHM 30% /ml ,KBM 60% /ml ; koloni 2 : zona hambatan 1,50 mm; KHM 30% /ml ,KBM 60% /ml; koloni 3 : zona hambatan 1,00 mm; KHM 30% /ml ,KBM 60% /ml; koloni 4 : zona hambatan 0,50 mm; KHM 30% /ml ,KBM 60% /ml; koloni 5 : zona hambatan 1,00 mm; KHM 30% /ml ,KBM 60% /ml; koloni 6 : zona hambatan 1,00 mm; KHM 30% /ml ,KBM 60% /ml;Kesimpulan: Secara in vitro, infusum kismis dengan konsentrasi 30% bersifat bakteriostatik, sedangkan pada konsentrasi 60% bersifat bakterisid dengan rata-rata Zona hambatan 1,0625 mm.

---

Background : Seedless Raisins has been known that it can inhibit the growth of pathogen bactery, because it contains of oleanolic acid that can inhibit the growth of oral pathogen.Objectives: The aim of the study is to determine the sensitivity of Infusum Raisins on mutans streptococci.Methods: Infusum is the product of the process of steeping Raisins for extraction of its medicinal principle. The effect of infusum Raisins was examined in vitro on the inhibit the bacterial growth by determining the inhibition zone (agar diffusion method), minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). The microorganisms tested were composed 6 colony of Streptococcus mutans wild strain that taken from Oral Biology Laboratory of Faculty of Dentistry University of Indonesia, labeled as Streptococcus mutans1, Streptococcus mutans2, Streptococcus mutans3, Streptococcus mutans4,Streptococcus mutans5, Streptococcus mutans6. Data obtained was done in a descriptive method.Results: showed that Raisins?s Infusum had effect on all of mutans of Streptococcus mutans 1 (inhibition zone 1.00 mm; MIC 30% /ml ,MBC 60% /ml); Streptococcus mutans 2 (inhibition zone 1.50 mm; MIC 30%/ml ,MBC 60%/ml); Streptococcus mutans 3 (inhibition zone 1.00 mm; MIC 30%/ml ,MBC 60%/ml); Streptococcus mutans 4 (inhibition zone 0.50 mm; MIC 30%/ml ,MBC 60%/ml); Streptococcus mutans 5 (inhibition zone 1.00 mm; MIC 30%/ml ,MBC 60%/ml), Streptococcus mutans6 (inhibition zone 1.00 mm; MIC 30/ml ,MBC 60%/ml).Conclusion: We concluded that Raisins's Infusum has anti microbial activity against 6 colony of Streptococcus mutans in oral cavity, in vitro. Hence it may have potential anti-cariesproperty."

"Latar Belakang : Jambu air Semarang (Syzygium samarangenase) atau jambu cincalo telah terbukti dapat menghambat pertumbuhan bakteri patogen, karena mengandung senyawa Tannin dan Oleanolic acid.Tujuan: Penelitian ini untuk membuktikan daya antimikroba infusum Jambu air Semarang terhadap Streptococcus mutans.Metode: Infusum Jambu air Semarang dibuat dengan proses pemanasan 100o C selama 15 menit terhadap 50 gram jambu air semarang dalam 500 ml air, kemudian disaring untuk mendapatkan 500 ml larutan (konsentrasi 10%), dipanaskan lagi sehingga larutan tersisa 50 ml (konsentrasi 100%), untuk penelitian ini dibuat infusum 80%, 60%, 40%, 30%, 20%, dan 15% sesuai prosedur yang benar. Efek antimikroba masing-masing konsentrasi infusum diperiksa dengan metode difusi serial dilusi sehingga diperoleh nilai KHM dan KBM serta metode difusi sehingga diperoleh nilai zona hambatan terhadap 6 koloni S.mutans.Hasil: Terhadap ke-6 koloni S.mutans diperoleh hasil sebagai berikut: KHM : 80%/ml dan KBM tidak diketahui serta rata-rata zona hambatan 1,533 mm.Kesimpulan: Secara in vitro, Infusum Jambu air Semarang dengan konsentrasi 80% berkhasiat menghambat pertumbuhan bakteri S.mutans(efek bakteriostatik).

---

Background : Wax apple (Syzygium samarangenase) has been known to prevent the growth of pathogen bacteria since anciety because it is contain fenol (tannin) and oleanolic acid which had been proved to prevent the growth of bacteria.Objectives: This research are for determine the antimicroba activity of Wax apple?s infusum on Streptococcus mutans.Methods: Wax apple?s infusum was made by the process of steeping seedless 50 gram Wax apple in 500 ml water, to see it?s medicinal properties after getting 100% concentration of solution. After that we made 80%, 60%, 40%, 30%, 20%, and 15% infusum. The antimicrobial activity of wax apple?s infusum was examined by dilution method to get the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC), and diffusion method to get the inhibition zone to 6 colony of S.mutans. Data obtained from this research in a descriptive method.Results: Effect of Wax apple?s infusum on Streptococcus mutans are : Streptococcus mutans type 1 inhibition zone 1,5 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutans type 2 inhibition zone 1,5 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutans type 3 inhibition zone 1,4 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutans type 4 inhibition zone 1,6 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutans type 5 inhibition zone 1,7 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutan type 6 inhibition zone 1,5 mm; MIC 80% /ml ,MBC unknown;Conclusion: We conclude that Wax apple?s Infusum has anti microbial activity against Mutans Streptococci, in vitro. Hence it may have potential anticariesproperty."

"S.mutans dikatakan sebagai salah satu penyebab utama karies. Bakteri ini dinyatakan sebagai bakteri pertama yang dapat melekat dan berkoloni pada permukaan gigi dan menyebabkan plak terbentuk secara terus menerus, dan terjadinya penurunan pH plak. Probiotik adalah suatu mikroorganisme hidup yang apabila dipergunakan dalam jumlah yang cukup, memberikan manfaat kesehatan bagi host. Berdasarkan berbagai penelitian, berbagai produk probiotik dapat mempengaruhi bakteri-bakteri penyebab karies gigi, terutama S.mutans. Penelitian ini dilakukan untuk mengetahui perbedaan jumlah koloni S.mutans dalam plak anak sebelum dan sesudah kumur minuman probiotik. Pengambilan sampel plak dilakukan terhadap 13 subyek dan dilakukan pertama kali yaitu sebelum memulai kumur minuman probiotik. Setelah itu subyek diinstruksikan untuk kumur minuman probiotik selama 7 hari dan pada saat hari ke 3 dan ke 7 kumur minuman probiotik sampel plak diambil kembali. Hasil penelitian memperlihatkan penurunan jumlah koloni S.mutans dari sebelum kumur minuman probiotik, kemudian pada hari ke 3 kumur, hingga setelah kumur minuman probiotik selama 7 hari. Hasil perhitungan statistik menunjukkan bahwa kumur minuman probiotik selama 3 dan 7 hari dapat menurunkan jumlah koloni S.mutans dalam plak gigi anak secara bermakna dibanding dengan sebelum kumur (p = 0,001).

---

S.mutans is said as one of the major etiology of caries. This bactery is said to be the first bactery that sticked and colonized on the tooth surface and caused the continuity of plaque formation, also the decrease of plaque?s pH. Probiotic is living microorganisms that, if used in adequate amount, will give health benefits to the host. Based on previous researches, various products of probiotic can influence caries etiology bacterias, especially S.mutans. The aim of this study is to know the differences of S.mutans colonization total amount before and after rinsing with probiotic drink. The plaque samples were first taken from 13 subjects before starting the probiotic oral rinse. After that subjects were instructed to rinse with probiotic drink for 7 days, and then in the 3rd and 7th days of rinsing, the plaque samples were taken again. The study showed that after 7 days rinsing with probiotic drink, the total amount of S.mutans colonization was found decreasing on the 3rd day and continued to the 7th day. Statistic count showed that rinsing with probiotic drinks for 3 and 7 days can make a significant difference on the amount of S.mutans colonization than before rinsing (p = 0,001)."